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All Journal Jurnal Ilmu Dasar Jurnal Ilmu Kedokteran (Journal of Medical Science) Majalah Kedokteran Bandung AL KAUNIYAH Journal of Agromedicine and Medical Sciences Biology, Medicine, & Natural Product Chemistry Elkawnie MNJ (Malang Neurology Journal) Medical Laboratory Technology Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Syntax Literate: Jurnal Ilmiah Indonesia JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jember Medical Journal (JMJ) International Journal of Health and Information System (IJHIS)
Sheilla Rachmania, Sheilla
Department Of Histology, Faculty Of Medicine, University Of Jember, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Journal : Medical Laboratory Technology Journal

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Differential Leukocyte Count Responses Post Injection of Duffy-binding-like Domain-2β of PfEMP1 Recombinant Protein in Wistar Rat Zahniar, Zahniar; Sulistyaningsih, Erma; Rachmania, Sheilla; Dewi, Rosita; Kusuma, Irawan Fajar
Medical Laboratory Technology Journal Vol. 11 No. 1 (2025): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i1.628

Abstract

Malaria due to Plasmodium falciparum causes a high mortality rate, and vaccination is a valuable approach to control it. One malaria vaccine candidate is Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1), specifically Duffy binding-like 2β (DBL2β) domain (DBL2β-PfEMP1), which has a vital role in severe malaria pathogenesis. The DBL2β-PfEMP1 recombinant protein is immunogenic. This study aimed to investigate the immune response of DBL2β-PfEMP1 protein by analyzing the differential leukocyte count. Twenty-three rats were randomly divided into control and five treatment groups. Rats were injected on days 0, 21, and 42 with a physiological solution of 0.9% NaCl, adjuvant, DBL2β-PfEMP1 protein, and each mixture of DBL2β-PfEMP1 protein with doses of 150, 300, and 450 µg/200gBW and adjuvant. Blood was collected on day 56 and prepared for differential leukocyte count examination with a visual microscopic examination by two expert observers. The results showed that DBL2β-PfEMP1 recombinant protein and adjuvant increased the eosinophils and neutrophils but decreased monocytes and lymphocytes and did not affect the basophils. Statistical analysis showed significant differences between groups for eosinophils (between control and DBL groups; Adj and DBL groups; DBL and other groups except DBL150+adj) and monocytes (between control and all doşe groups with adjuvant; DBL and all doşe groups with aduvant), but not for basophils, neutrophils, and lymphocytes. In conclusion, the serial injection of DBL2β-PfEMP1 recombinant protein showed different responses in each leukocyte cell type. Further analysis by time-series differential leukocyte count examination will be essential to determine the responses of each type of leukocyte to support the research on malaria vaccine development.
Co-Authors Aditha Satria Maulana Alami, Eqiel Navadz Akthar Ancah Caesarina Novi Marchianti Andriani, Made Prasanti Angga Mardro Raharjo, Angga Mardro Arsyzilma Hakiim Ayu Munawaroh Ayu Munawaroh Aziz, Ayu Munawaroh Bachtiar Hidayat, Aldy Bintang, Muhammad Farrel Ravidinata Masoga Caesarina, Ancah Cholis Abrori Dina Helianti Dwita Aryadina Rachmawati Erfan Efendi Erma Sulistyaningsih Erma Sulistyaningsih Erma Sulistyaningsih Gianevan, Nicholas Jirezra Hibatullah, Muhammad Naufal Ida Sri Surani Wiji Astuti Ida Srisurani Wiji Astuti Inke Kusumastuti Irawan Fajar Kusuma Irawan Fajar Kusuma Izza Amalia Putri Jauhar Firdaus Kholifaturrohmah, Sakinatus Sariroh Kholifaturrohmy, Muhammad Rizqi Lusi Padma Sulistianingsih Mata Luthfiyyah, Nur Atikah M. Ali Shodikin Muhammad Afiful Jauhani Muhammad Hasan Mukhammad Arif Hadi Khoiruddin Nizar Fiska Bayu Agustian Nofica, Cheila Nurmaida, Eny Putri, Elvia Rahmi Marga Rahma, Nafisah Hani Asyifah Rena Normasari Rini Riyanti Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi Rumastika, Nindya Shinta Sahrir Sillehu Sa’diyah, Nindya Audatus Sudarmanto, Yohanes Sulistyaningsih , Erma Sumadi Suryono Suryono Yunita Armiyanti Zahniar, Zahniar