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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bimbingan dan Konseling Jurnal Biologi Universitas Andalas Psikis : Jurnal Psikologi Islami Bioeksperimen: Jurnal Penelitian Biologi BioCONCETTA Jurnal Counsellia JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Biologi Tropis Jurnal Hilirisasi IPTEKS (JHI) JURNAL ILMU PENDIDIKAN AHLUSSUNNAH Jurnal Pengabdian kepada Masyarakat Nusantara Andalasian International Journal of Agricultural and Natural Sciences
Nofrita Nofrita
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Public Health Social Sciences Veterinary Other

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DNA Barcoding and eDNA Metabarcoding for Identification Species: A Case Study (West Sumatra): DNA barcoding and eDNA metabarcoding Roesma, Dewi Imelda; Tjong, Djong Hon; Syaifullah, Syaifullah; Nofrita, Nofrita; Janra, Muhammad Nazri; Aidil, Dyta Rabbani; Prawira, Furqan Dwiki Lintang; Salis, Viola Mutiara
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

The biodiversity of freshwater fish is important to study because there is data and information that remain undiscovered. The waters of Sumatra, especially West Sumatra, are areas with high freshwater fish diversity but have limited information. Providing information and genetic data has become one of the important things to conduct. DNA barcoding and eDNA metabarcoding have become molecular methods for identifying species and providing information about the presence of species in a region. A study using DNA barcoding and eDNA metabarcoding was conducted on freshwater fish in several locations in West Sumatra. Isolation and amplification of DNA were performed directly on individual samples and sequenced using conventional methods (Sanger sequencing) to generate DNA barcodes. Water samples were collected (2 liters) at each location using a sterile bottle. The water samples were filtrated, isolated, and amplified using universal primer and sequenced with next-generation sequencing techniques. The study successfully collected 25 species belonging to 14 genera, 2 families, and 1 order. A total of 134 sequences from West Sumatra with a length of 648-670 bp were analyzed. All DNA barcodes were submitted to the BOLD System and GenBank, NCBI. The mean Kimura two-parameter model (K2P) genetic distances within species, genera, families, and orders were 0.7%, 8.3%, 15.8%, and 21.3%, respectively. The eDNA metabarcoding technique has successfully detected three native fish species in the waters of West Sumatra (Barbonymus schwanefeldii, Mystacoleucus padangensis, and Rasbora jacobsoni). The availability of fish DNA barcodes in reference databases is crucial for the success of identification using eDNA metabarcoding. Combining identification using conventional methods and eDNA metabarcoding can provide more reliable results and become a reference for future freshwater monitoring.
Co-Authors . Mansyurdin A.A. Ketut Agung Cahyawan W Abd. Rasyid Syamsuri Afrizal Sano Ahmad Yazid Aidil, Dyta Rabbani Aini, Wardahtul Alfaiz Alfaiz Asful, Ferdhinal Ashrifurrahman, Ashrifurrahman Asmaiwaty Arief Aszareta, Muhammad Andoni Aziz, M. Abdul Bayu Marsha. Ds Bintari, Andhani N. Bintari, Andhini Nur Bramadi Arya BUSTANUL ARIFIN DAFIT SATRIA Dapit Pernalis Deden Tri Sang Putra Destaria Sudirman Nurhaty, Destaria Sudirman Dewi Imelda Roesma Djong Hon Tjong Dosi Juliawati EFATRICIA NIATI Efrizal Efrizal Eka Sefputri Elmayyeti Elmayyeti Elvi Susanti Eti Yerizel Fadillah Fadillah Fadillah Fadjar Goembira Faiz, Alfaiz Fani Karmila Fitra, Ramadani Fitri Yumilda Fitria Kasih Frenadin Adegustara Gustia Manasari Hamdi Ibrahim, Muhammad Hamida Hamida Hasneli, Hasneli Hengki Yandri Henny Herwina Idris, Muhamma Ilham Samudra, Muhammad Indra Ibrahim Irvan Apandi Ismi Auldra Efendi IYAS IYAS Izmiarti Izmiarti, Izmiarti Jabang Nurdin Janra, Muhammad Nazri Lasma Farida Luzi Ratnasari M. Haris Maliza, Rita Marchellino Irwan, Reziq Marisa Nanda Marwisni Hasan Melda Krisna Dasvita Mellyarti Mellyarti Mellyarti Syarif Mhd Nur Allatif Muhammad Afif Muhammad Idris Muhammad Idris Muhammad Nazri Janra Murisal Murisal muryani, nova Musda Rukmana Melka Nasution, Risda Afifi Nazri Janra, Muhammad Nefri Yanra Niki Yulita Herman Nola Karmila Sari Noliza Noliza Novia Susana Novita Ratna Sari Olly Norita Tetra Pratama Elisa, Tasya Putri Pratama, Raihan Anugrah Prawira, Furqan Dwiki Lintang Puji Yani Pratama Putra Santoso Putri Pratama Elisa, Tasya Putri, Eggy Triana Putri, Syntia Mai r maliza, rita Randi Saputra Reski Puspita Sari Ridho, Muhammad Syifa'ur Rila Rahma Mulyani Rini Septiani Yunas Robby Jannatan Ryan Hidayat Rafiola Salis, Viola Mutiara Saniyyah, Jauharah Sari Murni Sari, Azmatul Khairiyah Savitri, Vivi Silva Lestari Sinurat, Arthauly Yopita Sulis Setiawati Sumartin, Harry Syaifullah Syaifullah Taufik Hidayatulloh Taufiq Ihsan Tofrizal Tofrizal, Alimuddin Wilson Novarino Winda Rahayu Yasir Muharram Fauzi Yelvi Monasari Yulinar Yulinar Yuzarion, Yuzarion Zaini Zaini