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All Journal Jurnal Veteriner Journal Of Vocational Health Studies Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Darmabakti Cendekia: Journal of Community Service and Engagements Journal of Applied Veterinary Science and Technology
Diyantoro Diyantoro, Diyantoro
Department Of Health, Faculty Of Vocational Studies, Universitas Airlangga, Surabaya-Indonesia
Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Novel Surface Marker for the Prospective Mesenchymal Stem Cell Characterization from Rabbit Visceral Adipose Tissue Kuncorojakti, Suryo; Yudaniayanti, Ira Sari; Susanti, Lina; Susilawati, Helen; Aswin, Ahmad; Diyantoro, Diyantoro; Rodprasert, Watchareewan
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.1-9

Abstract

Rabbit as a laboratory animals play an important role in bridging basic research to clinical application. The exploration of rabbit mesenchymal stem cell (MSC) is still facing an obstacle regarding the standardization of characterization of MSC. This study is aimed to explore the novel candidate of rabbit MSC surface marker as an effort to establish the goal standardization of rabbit MSC. Three 2-month-old male White New Zealand rabbits weighing 2 kg were used as visceral rabbit adipose derived mesenchymal stem cell (Rab-ADMSC) donors. The cells were characterized according to their morphology characteristic, clonogenic and three-lineage differentiation capacity. Flow cytometry was used to evaluate the MSC surface markers of Rab-ADMSC against CD81, CD29, CD34 and CD45. The results of this study revealed that the Rab-ADMSC posses characteristic of MSC according to their morphology and differentiation capacity. It can be concluded that CD81 surface marker can be proposed as a stable alternative candidate marker for rabbit MSCs. This data is supported by other phenotypic characteristics of MSCs, both in morphology and in the ability of visceral Rab-ADMSCs to differentiate into adipogenic, osteogenic, and chondrogenic lineages.
Identifikasi Kandungan Komponen Babi pada Daging Curah dan Produk Olahan Daging Menggunakan Metode ELISA Sandwich di Balai Besar Veteriner Wates Meilinia, Shafina Meilinia; Achmad, Agung Budianto; Diyantoro, Diyantoro; Chrismanto, Dony
VITEK : Bidang Kedokteran Hewan Vol. 11 No. 2 (2021): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v11i2.78

Abstract

Background: Indonesians have relatively high dietary requirements. Meat and processed items are frequently counterfeited in today's world. Pork is a low-cost protein provider that is cheap and readily available. As a result, some traders began substituting the whole meat and processed products with pork. In protecting the community, BBVet Wates conducted ELISA Sandwich testing. Purpose: To find out the addition of pork content in meat and processed products and the effectiveness of ELISA for species tests. Method: ELISA type sandwich. Result: In 2019, there were 45 positive samples and 113 negative samples, while in 2020, there were 23 positive samples and 59 negative samples. Conclusion: In 2020, there will be a decrease in cases of pork addition by 0.45% from 2019. The Elisa method species test has high accuracy and sensitivity. Keywords: Species Test; ELISA; Meat and Processed Products.
Co-Authors A'la, Rofiqul Agung Budianto Achmad, Agung Budianto Agus Widodo Agus Widodo Aswin, Ahmad Bambang Sektiari Lukiswanto Boedi Setiawan Brillian Inawulan Syamsiar Chrismanto, Dony Dalila Fadhila Hidayat Delaiah, Diena Dwi Wahyu Indriati Eko Sugeng Pribadi Fadilatus Sukma Ika Noviarmi Fedik Abdul Rantam Gandul Atik Yuliani Helen Susilowati, Helen I wayan Teguh Wibawan Indriati, Dwi W. Ira Sari Yudaniayanti Ira Sari Yudaniayati Kuncoro Puguh Santoso Kuncorojakti, Suryo LINA SUSANTI Lucia Tri Suwanti, Lucia Tri Maulana, Firdausy Kurnia Meilinia, Shafina Meilinia Myrna Adianti Ni Nyoman Purwani Nur Septia Handayani Prasetya, Tofan Agung Eka Rahmahani, Jola Rizka Oktarianti Ainun Jariah Rodprasert, Watchareewan Septriana, Maya Sofyan, Miyayu Soneta Sundari, Aliyah Siti Susilawati, Helen Syamsiar, Syamsiar Widyaningrum, Lilik N. Wijaya, Andi Yasmin Wulandari, Shelly Yeni Dhamayanti Yulianna Puspitasari Yusril Nur Mahendra