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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

SHAKING RED CAP-BLOOD COLLECTION TUBE WITHOUT ADDITIVE SUBSTANCES IS RECOMMENDED TO ACCELERATE THE BLOOD CLOTTING PROCESS Gilang Nugraha; Rohayati Rohayati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i1.913

Abstract

Many blood collection tube manufacturers do not recommend shaking the red tube. Shaking the red tube to avoid the intensity of the changed of blood that interacts with the glass surface of the tube will trigger the contact path in the coagulation cascade. Generally, the blood takes 30-60 minutes to clots at room temperature without shaking. The aim of this study was to determine the effectiveness of shaking the red-cap blood collection tube in producing serum volume. The method of this study was experimental As much as 5 mL of blood was taken and put into 3 tubes with a volume of 1mL each tube. The first and the second tube were shaken 8 times. The first tube was incubated for 10 minutes while the second tube was incubated for 25 minutes. Meanwhile, the third tube (as a control) was not shaken but was incubated for 40 minutes. The tube were centrifuged at 3000 g for 10 minutes. The serum volume was measured using micropipette and collected into Eppendorf tube. The results showed that there were a difference in the number of serums formed after tube shaking by time variation (P = 0.002), the results of the Post Hoc Test using Bonferroni test while showed that the second tube did not have a difference in serum volume with control (P = 0.751). It can be concluded that the red-cap blood collection tube, which was shaken 8 times and incubated for 25 minutes long could accelerate the coagulation process.
STUDY OF HEMOGLOBIN LEVELS ON HEMOLYSIS SAMPLE Noor Febryani; Ikke Nanda Amalia; Intan Dwi Anggraeni; Gilang Nugraha
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1311

Abstract

Hemolysis can significantly affect the reliability of test results and occur in the pre-analytical phase. The aim of this study is to reveals the correlation of hemoglobin levels on hemolysis sample. This experimental study was carried out using samples from thirty students of Medical Laboratory Technology study program of the Nahdlatul Ulama University of Surabaya. Blood samples were treated by hard shaken in 30 times in order to damage the middle part of the blood sample. Data on hemoglobin levels were collected and analyzed using the Pearson Correlation Test before and after treatment with significant value p < 0.05 indicating that there was a significant correlation. Hemoglobin concentrations were strongly positive for the hemolysis of the sample (p = 0.000). The conclusion is that sample hemolysis has a potency to be the confounding factor on the hemoglobin test.
Co-Authors Abdul Muhith Abraham Ahmad Ali Firdaus Ahmad Jazuly Nabil Akhmad Rizal Effendi Alfia, Shofa Alya Salsabila Andini, Ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Ardyarini Dyah Savitri Ariska Widya Arsya Tazkiya Asror, Anita Rizki Rahmawati Atika Nur Laila Aulia, Ilmiah Alvi Nisa Ayu Slatim Maifanda Ballihgoo, Hamiduumajid Ballihg Bermawi, Bastiana Brina Thursina Dibiasi Budiarti, Rina Devyana Dyah Wulandari Diyan Wahyu Kurniasari Dwi Handayani Dwi Lestari Pertiwi Dwijayanti, ira Edhi Rianto Edijanto, Soebagijo Poegoeh Edijanto, Subagijo Poegoeh Edjianto, Soebagijo Poegoeh Effendi, Akhmad Rizal Ersalina Nidianti Fakurazi, Sharida Fauziah, Luqiana Hakim, Muhammad Yusuf Ramadhani Hermawati, Septina Isro’ Hidayat, Muhammad Taufiq Hidayatih, Nurul Ikke Nanda Amalia Ilmiah Alvi Nisa Aulia Intan Dwi Anggraeni Ira Dwijayanti Janetra Yudhis Nata P Kurniasari, Diyan Wahyu Laila, Atika Nur Layla Agustina, Nurul Ivada Maghfuroh, Qonita Iklila Maritza, Phasa Shoofii Luthf Marselli Widya Lestari Maulana Bagas Syaifulloh Mirshall Abiedama Prayoga Moch. Sahri Mohammad Nasir Muhamad Damar Baskara Muhammad Afwan Romdloni Muhammad Nasir Mukarramah, Imraatul Murdiningsih, Eka Indah Nastasya Nunki Nathalya Dwi Kartika Sari Navis, Khiliah Nila Dwi Utami Noor Febryani Nugraha, Dayu Satria Nur Anita Ningsih Nur Masruroh Nur Masruroh Nur Masruroh, Nur Nur Muhamad Nuzulul Syufi Nur Zalsabila Isnaini Prayekti, Endah Putri, Dwi Yuniar Nandita Rachmayanti, Notrisia Rahayu Anggraini Rahayu, Mita Rahmawati, Lili Susan Rizka Wahyu Safitri Rizki Nurmalya Kardina Rohayati Rohayati Rosa Sastrawati Rosda Rodhiyana Rodhiyana Ruri Arinil Khoiro S. P. Edijanto Penulis Salimi, Syafrina Maulidyatus Salman Firmansyah Salsabila, Alya Salsabillah Karunia Sugiarto Saumia Khoirunnisa Syadzila Siegfried Syafier, Siegfried Siti Husnul Khotimah Siti Rosa Lina Sitti Fitria Sri Surya Suciati Subagijo Poegoeh Edijanto Suciati, Sri Surya Sumarsono, Thomas Syadzila, Saumia Khoirunnisa Syinta Nur Isnaini Thomas Sumarsono Titik Sulifah Umi Hanik Utami, Nila Dwi Widya Novita Sari Wieke Sriwulan Wijanarko, Robert Aditya Wulan, Wieke Sri Zalfa, Anisatuz