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Microwave-Assisted Deep Eutectic Solvent Extraction of Lipids from Ulva sp.: Optimization and Fatty Acid Profiling Rizkiana, Meta Fitri; Lestari, Afrila Tutut Dwijati; Sholeha, Irdatus; Palupi, Bekti; Putri, Ditta Kharisma Yolanda; Rahmawati, Istiqomah; Amini, Helda Wika; Fachri, Boy Arief
Jurnal Pijar Mipa Vol. 20 No. 5 (2025)
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v20i5.9588

Abstract

Ulva sp. is a green alga commonly found in Indonesian waters. It contains approximately 60% carbohydrates, 10–47% protein, and 1–3% lipids. This study aimed to determine the optimum conditions for lipid extraction from Ulva sp. A pre-treatment step was carried out by adding a Deep Eutectic Solvent (DES) and distilled water. The DES used was a mixture of zinc chloride and urea in a 1:2 molar ratio. The sample was then mixed with a chloroform-methanol solvent (2:1 v/v) and subjected to Microwave-Assisted Extraction (MAE). The variables investigated in this study included microwave power (150, 300, and 450 W), extraction time (10, 15, and 20 min), and solvent volume (45, 60, and 75 mL). The organic phase obtained from the extraction process was separated and evaporated to determine the lipid yield. The highest yield, 15.8%, was achieved under conditions of 20 min extraction time, 75 mL solvent volume, and 300 W of power. GC-MS analysis of the highest-yield sample revealed the presence of fatty acids including palmitic acid, oleic acid, and palmitoleic acid. This optimized method supports future applications in biodiesel production and green extraction processes for algae-based bioresources.
Optimization of Rotation Speed, Disc Diameter, and Lighting Time in Batik Waste Treatment Using Rotary Algae Biofilm Reactor (RABR) with Ulva sp. Raharjo, Sonya Hakim; Palupi, Bekti; Syaifullah, Rangga Yudha; Yohanes; Sekaringgalih, Ratri; Hidayati, Nurul
Eksergi Vol 22 No 3 (2025)
Publisher : Prodi Teknik Kimia UPN "Veteran" Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/eksergi.v22i3.15084

Abstract

Batik is one of the cultural heritages in Indonesia that must be maintained and preserved. The batik industry process itself produces liquid waste that comes from the coloring processing, washing, wax removing, also rinsing. Batik waste, if not treated properly, can harm the environment. Various studies have shown that effluent treatment using the Rotary Algae Biofilm Reactor (RABR) method is promising. This research focuses on improving the RABR design and optimal conditions for treating batik wastewater, as well as utilizing the synergy between batik production and Ulva sp. The variables used in this research are the rotation speed of 20, 30, and 40 rpm, the lightning time for 0, 6, and 12 hours, and the disk diameter size of 9, 11, and 13 cm. The parameters that analyzed are BOD, COD, and pH levels. Waste treatment optimization in this research uses the RSM with a combination of Design Expert 13 software. Based on the results, the most optimal batik wastewater treatment variable is when the disk diameter is 10.306 mm, the rotation speed is 20 rpm, and the lightning time is 7.805 hours, yielding response values of 55.673 mg/L for BOD, 25.538 mg/L for COD, and 10.406 for pH.
Optimization of Xylitol Production from Tobacco Stem Using Ultrasound-Assisted Acid Hydrolysis Coupled with Fermentation Yukti Nurani; Bekti Palupi; Diana Madhuri; Diah Tri Wulandari; Ansori Ansori
INSOLOGI: Jurnal Sains dan Teknologi Vol. 4 No. 4 (2025): Agustus 2025
Publisher : Yayasan Literasi Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55123/insologi.v4i4.6084

Abstract

Tobacco (Nicotiana tabacum L.), as one of the main commodities in East Java Province, where only the leaves are utilized, while the stems become agricultural waste and are often burned, contributes to air pollution. Nonetheless, these tobacco stems possess potential for high-value chemical products, such as xylitol. This study investigated the optimum operating conditions for producing xylitol from tobacco stem waste. The process employed Ultrasound-Assisted Acid Hydrolysis followed by fermentation using Candida tropicalis. Hydrolysis was conducted in an ultrasonic bath that operates at 40 kHz and 50 watts, using 0.1 N H₂SO₄ as the solvent. The resulting hydrolysate was then fermented by adding Candida tropicalis (20 mL per 100 mL substrate) and incubating for 48 hours, 30 °C, 120 rpm shaker. Optimization was conducted utilizing Response Surface Methodology method to assess the interactions between operating variables (temperature, time, and volume of solvent during hydrolysis) and xylitol yield. The study investigated the optimum operating conditions to produce xylitol were 30 °C, 50 minutes, and 150 mL of solvent, yielding xylitol at a concentration of 5.6 g/L.
The Optimization of Chlorophyll Extraction from Ulva Sp. with Ultrasound-Assisted Liquid Biphasic Systems Method Susilowati; Humaidi, Fakhri Arkaan; Fachri, Boy; Palupi, Bekti; Rizkiana, Meta Fitri; Rahmawati, Istiqomah; Amini, Helda Wika
Indonesian Journal of Science and Education Vol. 8 No. 1 (2024): Indonesian Journal of Science and Education
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/ijose.v8i1.1085

Abstract

Ulva sp. is a type of green algae that is easily found in the shallow seas of Indonesia and contains various bioactive compounds. Chlorophyll is a bioactive compound that functions as a natural dye, free radical scavenger, and antioxidant in the body. Chlorophyll extraction with conventional methods requires a relatively long time and large amount of solvent. In this research, chlorophyll extraction from Ulva sp. with the Ultrasound-Assisted Liquid Biphasic System (UALBS) method. Ulva sp. as much as 2.5 grams mixed in acetone solvent, added K2HPO4 solution, and carried out the sonification process in a dark room. The extraction process performs at a parameter range of 5-15 minutes, ratio 0.05-0.1 g/mL, and 60-100 mesh particle size. The extracted filtrate was added with petroleum ether and distilled water to form a biphasic condition. The extracted chlorophyll was analyzed with chromatography and spectrophotometry method. Antioxidant activity was analyzed using the DPPH method. The optimum result of Ulva sp. chlorophyll extraction at an extraction time of 10 minutes, the ratio of solids to solvent was 0.1, the particle size of 60 mesh produced a yield of 1.88% with chlorophyll a 20.13664%, chlorophyll b 21.58672% and total chlorophyll 41.71012%, and the percentage inhibition 45.32%.
EKSTRAKSI PROTEIN DARI SPIRULINA SP DALAM PELARUT ETANOL TERBANTUKAN GELOMBANG ULTRASONIK Fachri, Boy Arief; Palupi, Bekti; Rahmawati, Istiqomah; Rizkiana, Meta Fitri; Amini, Helda Wika
Jurnal Penelitian Sains dan Teknologi Indonesia Vol 1 No 1 (2022): Jurnal Hasil Penelitian UNIVERSITAS JEMBER
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jpsti.v1i1.90

Abstract

Protein merupakan komponen nutrisi yang dibutuhkan oleh manusia untuk kesehatan tubuh. Salah satu sumber protein yang melimpah di Indonesia adalah alga Spirulina Sp. Ekstraksi protein alga, sulit dilakukan. Hal ini dikarenakan, protein tersimpan dalam sel-sel alga yang memiliki dinding yang sangat keras. Metode konvensional yang selama ini dilakukan membutuhkan waktu yang sangat lama, dan energi yang besar. Untuk mengatasi hal itu, sebuah metode alternatif yang diajukan dalam penelitian ini, adalah metode ekstraksi ultrasonik. Tujuan penelitian ini adalah untuk menentukan kondisi optimum ekstraksi protein dari Spirulina Sp dan mengetahui pengaruh parameter ekstraksi terhadap yield. Sprirulina Sp yang tekah dikeringkan dan dihaluskan diumpankan ke dalam ekstraktor yang berisi etanol. Gelombang ultrasonic, kemudian dialirkan ke dalam ekstraktor melalui probe. Setelah, eaktu ekstraksi tercapai, ekstrak dan residu dipisahkan dengan cara sentrifugasi dengan kecepatan 10000 rpm pada suhu kamar. Selanjutnya, yield protein ekstrak dihitung dengan analisa Lowry-Bradford. Pengaruh parameter proses seperti rasio bahan baku-pelarut (0:1-0.5) g/mL, ukuran partikel bahan baku (600-800) mesh dan waktu ekstraksi 15-45 menit) terhadap yield akan diamati dalam penelitian ini. Berdasarkan hasil penelitian, dapat disimpulkan bahwa yield tertinggi sebesar 80,87 % diperoleh pada kondisi rasio bahan baku-pelarut 0.2 g/mL, ukuran partikel bahan baku 700 mesh dan waktu ekstraksi 30 menit. Sementara itu, ukuran partikel merupakan parameter yang paling signifikan.
A Comparison of Unconventional Microwave and Ultrasound-Assisted Extraction Methods used for Flavonoids Rahmawati, Istiqomah; Azizi, Daffa Hafiziaulhaq; Wibowo, Jihan Nafila; Reza, Muhammad; Fachri, Boy Arief; Palupi, Bekti; Rizkiana, Meta Fitri; Amini, Helda Wika; Ramadana, Ifan; Setiawan, Felix Arie
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cocoa pods (Theobroma cacao L.) are a rich source of flavonoids, which are natural antioxidants known for their health benefits. This study investigated the use of microwave-assisted extraction (MAE) and ultrasound-assisted ex-traction (UAE) to extract the maximum flavonoids and antioxidants from cocoa pods. MAE and UAE are efficient and sustainable methods for extracting bioactive compounds like flavonoids and antioxidants from cocoa pods, offer-ing faster extraction, reduced solvent use, and better compound preservation compared to conventional methods. These technologies unlock the untapped potential of cocoa pods for applications in food, cosmetics, and pharmaceuti-cals. The effects of extraction time (2–10 min), microwave power (100–300 W), and the ratio of cocoa husk powder to solvent (0.02–0.06 g/mL) were evaluated for MAE. Meanwhile, for UAE, different temperatures (30–50 °C), times (10–30 min), and powers (16–48 W) were studied. The Design-Expert® software with Response Surface Methodology (RSM) and a Box-Behnken Design was utilized to analyze the effects of a combination of these parameters. The re-sults showed that extraction time, microwave power, and the ratio of cocoa husk powder–solvent significantly affected the total flavonoid yield and antioxidant capacity. The highest total flavonoid yield obtained by the MAE method (123.07 ppm) was at an extraction time of 10 min, cocoa husk–solvent ratio of 0.06 mg/mL, and microwave power of 300 W. The extract obtained by the MAE method showed an excellent antioxidant capacity of 43.49 mg/mL GAEAC, and a robust antioxidant activity indicated by an IC50 value of 42.19. Conversely, the highest total flavonoid yield of the UAE method (8.45 mg/mL GAEAC) was achieved at 30 min, 40 °C, and 40 W. The extract from the UAE method demonstrated a better antioxidant capacity (7.51 mg/mL GAEAC) and antioxidant activity (IC50 value of 23.46) than that from the MAE technique
Pemanfaatan Ulva sp. pada Sistem High Rate Algae Reactor (HRAR) untuk Pengolahan Limbah Cair Batik Palupi, Bekti; Megasari, Tri Elok Setya; Adelina, Saltsabita Nourin; Fachri, Boy Arief; Rahmawati, Istiqomah; Rizkiana, Meta Fitri; Amini, Helda Wika; Muharja, Maktum; Mumtazah, Zuhriah
Dinamika Kerajinan dan Batik: Majalah Ilmiah Vol. 41 No. 2 (2024): DINAMIKA KERAJINAN DAN BATIK : MAJALAH ILMIAH
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Industri Kerajinan dan Batik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22322/dkb.v41i2.8466

Abstract

Batik merupakan salah satu warisan budaya Indonesia. Dalam produksinya, batik menggunakan bahan pewarna, malam, dan zat-zat kimia lainnya. Penggunaan bahan tersebut menghasilkan limbah cair dengan kandungan kadar minyak dan lemak, COD, dan pH yang tinggi. Selain itu, limbah yang dibuang langsung ke lingkungan merupakan limbah yang beracun dan dapat merusak ekosistem. Metode High Rate Algae Reactor (HRAR) menggunakan Ulva sp. digunakan pada penelitian ini. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi tablet CO2, lama penyinaran, dan banyaknya suspensi Ulva sp. dalam pengolahan limbah. Ulva sp. sebanyak 5 – 15% dimasukkan ke dalam HRAR bersamaan dengan limbah cair batik, kemudian dilakukan penambahan tablet CO2 sebanyak 0,27 – 0,81 gram yang selanjutnya dilakukan penyinaran selama 6 – 24 jam. Optimasi pengolahan limbah dilakukan menggunakan metode RSM (Response Surface Methodology) dengan rancangan percobaan kombinasi perlakuan Central Composite Design (CCD) menggunakan software Design Expert 13. Berdasarkan hasil penelitian, variabel pengolahan limbah paling optimal berada pada saat konsentrasi suspensi Ulva sp. 5%, lama penyinaran 24 jam, dan massa tablet CO2 sebesar 0,27 gram. Respon yang diperoleh pada variabel tersebut yakni kadar COD 48,2 mg/L, kadar minyak dan lemak 0,432 mg/L, serta nilai pH 7,929.
Optimasi Ekstraksi Flavonoid dari Ulva lactuca dengan Kombinasi Cell Disruption dan Ultrasound-Assisted Hot Water Helda Wika Amini; Muhammad Rizky Kurniawan; Nida Ayu Salsabila; Boy Arief Fachri; Zuhriah Mumtazah; Meta Fitri Rizkiana; Istiqomah Rahmawati; Bekti Palupi; Yukti Nurani; Rekfa Wika Amini
ALCHEMY Jurnal Penelitian Kimia Vol 21, No 2 (2025): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.21.2.98141.284-295

Abstract

Ulva lactuca merupakan biota laut yang berpotensi sebagai sumber bahan aktif kosmetik karena kandungan bioaktifnya seperti flavonoid, alkaloid, saponin, terpenoid, dan tanin. Penelitian ini bertujuan mengekstraksi flavonoid serta menganalisis nilai Sun Protection Factor (SPF) U. lactuca menggunakan kombinasi metode cell disruption dan ultrasound-assisted hot water (UAHW). Tiga metode perlakuan cell disruption (tanpa perlakuan, osmotic shock, dan hidrotermal dengan autoklaf) dikombinasikan dengan variabel UAHW yaitu suhu ultrasonik (40, 60, 80 °C) dan waktu ultrasonik (5, 10, 15 menit). Ekstraksi dilakukan menggunakan pelarut etanol 96% pada serbuk U. lactuca berukuran 60 mesh. Optimasi dilakukan menggunakan Response Surface Methodology (RSM) dengan Box–Behnken Design (BBD). Hasil menunjukkan bahwa total flavonoid dan nilai SPF tertinggi (33,23 mg QE/g dan SPF 20,80) diperoleh tanpa perlakuan cell disruption, pada suhu 80 °C dan waktu ekstraksi 10 menit. Penelitian ini menegaskan bahwa metode UAHW efektif meningkatkan ekstraksi flavonoid dan SPF meskipun tanpa cell disruption. Selain itu, pendekatan RSM terbukti efisien dalam optimasi proses. Studi ini memberikan kontribusi pada pengembangan bahan aktif alami U. lactuca untuk aplikasi kosmetik, khususnya sebagai agen pelindung sinar UV.Optimization of Flavonoid Extraction from Ulva lactuca Using a Combination of Cell Disruption and Ultrasound-Assisted Hot Water. Ulva lactuca is a marine organism with significant potential as a source of active ingredients for cosmetic applications, owing to bioactive compounds such as flavonoids, alkaloids, saponins, terpenoids, and tannins. This study aimed to extract flavonoids and evaluate the Sun Protection Factor (SPF) of U. lactuca using a combination of cell disruption methods and ultrasound-assisted hot water (UAHW) extraction. Three cell disruption treatments (no treatment, osmotic shock, and hydrothermal using an autoclave) were combined with UAHW variables, including ultrasonic temperature (40, 60, 80 °C) and ultrasonic time (5, 10, 15 minutes). The extraction was conducted using 96% ethanol as a solvent on U. lactuca powder sieved to 60 mesh. Optimization was performed using Response Surface Methodology (RSM) with a Box–Behnken Design (BBD). The highest total flavonoid content (33.23 mg QE/g extract) and SPF value (20.80) were obtained without cell disruption, at 80°C for 10 minutes. The study confirms that UAHW effectively enhances flavonoid extraction and SPF, even without cell disruption. Furthermore, the RSM approach proved efficient for process optimization. This study contributes to developing natural active ingredients from U. lactuca for cosmetic applications, particularly as UV-protective agents.
Strategi Pengemasan dan Pemasaran Lilin Aromaterapi dan Sabun Cuci Piring dari Minyak Jelantah Anggraeni, Sella Violina; Naily Fatimatuz Zaroh; Amalia Ramadhan; Mochamad Ghaza Al Ghifari; Richa Febyola Surya Putri; Boy Arief Fachri; Bekti Palupi; Meta Fitri Rizkiana; Istiqomah Rahmawati
Dedikasi:Jurnal Pengabdian kepada Masyarakat Vol. 6 No. 1 (2025): Jurnal Dedikasi
Publisher : Universitas Jayabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31479/dedikasi.v6i1.423

Abstract

Desa Plalangan yang terletak di Kecamatan Kalisat, Kabupaten Jember, merupakan salah satu desa binaan Universitas Jember dengan potensi besar dalam pengembangan produk ramah lingkungan. Masyarakat desa ini sebelumnya telah dilatih untuk mengolah minyak jelantah menjadi sabun cuci piring dan lilin aromaterapi, namun aspek pengemasan dan pemasaran produk masih perlu ditingkatkan agar memiliki nilai jual lebih tinggi. Program pengabdian ini bertujuan untuk memberikan pelatihan strategi packaging dan marketing produk berbasis minyak jelantah agar masyarakat mampu mengembangkan usaha secara mandiri. Metode pelaksanaan meliputi pendalaman mutu produk, perancangan desain kemasan, pelatihan strategi pemasaran offline dan online, serta evaluasi tingkat pemahaman peserta. Hasil kegiatan menunjukkan peningkatan wawasan dan kreativitas warga dalam mendesain kemasan yang menarik dan fungsional, serta pemahaman mengenai cara promosi produk baik melalui penjualan langsung (face to face selling) maupun live selling di platform digital seperti TikTok. Program ini diharapkan dapat memperkuat daya saing produk lokal, meningkatkan pendapatan masyarakat, serta mendukung terwujudnya ekonomi sirkular berbasis lingkungan di Desa Plalangan.
Enhancing Biotechnology Literacy through Kombucha Tea Making Socialization at SMA Negeri 1 Giri Istiqomah Rahmawati; Bekti Palupi; Merymistika Yufrani Afred; Agus Wedi Pratama
Jurnal Inovasi Sains dan Teknologi Untuk Masyarakat Vol. 3 No. 2 (2025): November
Publisher : Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/instem.v3i2.4

Abstract

Biotechnology learning at the secondary school level is often dominated by theoretical approaches, potentially limiting students' understanding of real-life scientific applications. This community service activity aimed to implement the Tri Dharma of Higher Education through socialization and training on kombucha tea making at SMA Negeri 1 Giri, Banyuwangi. The primary objective of this program was to strengthen students' applied biotechnology literacy and foster an entrepreneurial spirit by introducing the business potential of fermented products. The implementation method employed a participatory approach involving 53 students as active participants on March 18, 2025. The activity stages included interactive material delivery regarding SCOBY (Symbiotic Culture of Bacteria and Yeast) microbiology and the health benefits of kombucha, followed by practical demonstrations covering the process from starter inoculation to fermentation. In addition to technical aspects, this activity specifically incorporated training on simple economic analysis, including calculations for raw material costs, production expenses, and the determination of competitive selling prices for a home-industry scale. The results indicated high participant enthusiasm, evidenced by active participation in discussion and quiz sessions. Students successfully gained a deep understanding of the biochemical reactions occurring during fermentation and the potential of kombucha as a functional beverage. Furthermore, the provided business insights transformed students' perspectives, highlighting the high economic value of biotechnology products. It is concluded that the integration of practical training and business insights effectively enhances students' scientific competence while supporting the implementation of the entrepreneurship theme within the Merdeka Curriculum.
Co-Authors Abdul Malik Karim Amrullah Adelina, Saltsabita Nourin Adrian, Febri Adriansyah, Muhamad Afred, Merymistika Yufrani Afwal, Diza Raudhatul Agus Wedi Pratama Aina Christalia Rinastiti Akbar, Muhammad Faqihuddin Akhsan, Maulana Yudha Al Fajri, Maktum Muharja Alifia Rahma Putri Neysella Amalia Ramadhan Amartya, Audi Nur Andikasari, Emi Anggraeni, Sella Violina Anggraini, Ranita Anggi Annisa, Yasmin ansori ansori Ardianti , Nanda Melani Ari Susanti Ari Susanti Ari Susanti Ariel Seanhan Haezer Asshofy , Muhammad Agil Atiqa Rahmawati Atiqa Rahmawati Atiqa Rahmawati AYU WULANDARI Aziz, Mohamad Naufal Nizaar Azizi, Daffa Hafiziaulhaq Badril Azhar Badril Azhar Bestari, Cherobina Bida Ayu Bima Suci, Syeikh Maulana Bimo Bayu Aji Boy Arief Fachri Boy Fachri Darojat, Zakiah Devara, Arighy Zahirah Faiqy Diah Tri Wulandari Diana Madhuri Dianavita Fatimah Dianita Ivana Permata Dihayat, Icha Shofia Dini Rahmawaty Ditta Kharisma Yolanda Ditta Kharisma Yolanda Putri Elisah, Nor Fachri, Boy Fakhri Arkaan Humaidi Fandora, Ardan Jauza Farwah, Zilma Aliyah Fatimah, Dianavita Felix Arie Setiawan Firdausi, Adnan Maulana Firmansyah, Alex Firmansyah, Arfian Alwi Firmansyah, Muhammad Sahrul Firsta Retnaningtyas Udroto Gregah Pangayoman Hartanto P Gregah Pangayoman Hartono Putro Habibatul Inayah Harti Arini Hakiki, Rizki Maulana Hanafi, Yogie Alfian Helda Wika Amini Helda Wika Amini Heni Pujiastuti Herdianto, Dimas Nur Hidayat, Debby Aurellia Humaidi, Fakhri Arkaan Indrayani, Lilin Intan Permatasari Istiqomah Rahmawati Istiqomah Rahmawati Kenji Sakai Kenji Sanomoto Khoirunnissa , Aulia Kurniawan, Muhammad Rizky Lailatul Krisna Abdullah Lestari, Afrila Tutut Dwijati Lukman Nulhakim Lumbantoruan, Sri Lestari Maharani, Regita Gustiayu Pramisti Mahfud Mahfud Mahfud Mahfud Maktum Muharja Maulida Septiyana Megasari, Tri Elok Setya Meta Fitri Rizkiana Mizanurafi’ Ghifarhadi Prasiefa Mochamad Ghaza Al Ghifari Mohammad Nor Muhammad Ilham Muhammad Reza Muhammad Reza Muhammad Rizky Kurniawan Muharja, Maktum Nadia Ayumna Fa’iqoh Naily Fatimatuz Zaroh Nanda Ayudiyah Andriani Nida Ayu Salsabila Nikita Meidi Nova Chintya Kurniawati Nova Chintya Kurniawati Nugraha, Briantara Agung Nugroho, Yeremia Prasetya Nur Karima Nur Karima Nurani, Yukti Nurjannah, Lailia Nurtsulutsiyah Nurtsulutsiyah Nurtsulutsiyah, Nurtsulutsiyah Nurul Hidayati Pradipta, Shima Nuril Pramudiska, Riavinola Viyoni Pratiwi, Widya Septaningtyas Pravitasiwi, Nanda Tricya Julia Prayuga, Devi Purimahua, Amanda Augre Maharani Putri, Salsabila Ananda Rachman, Darryl Akeyla Rafif, M. Attar Raharjo, Nadhya Cahya Dwy Rahman, Agus Ramadana, Ifan Ratnasonia, Samantha Githa Ratri Sekaringgalih Regita Gustiayu Pramisti Maharani Rekfa Wika Amini Relyando, Syahfa Adinda Reswara Musyafa Richa Febyola Surya Putri Rizalluddin, Muhammad Rizki Fitria Darmayanti Rizki Fitria Darmayanti Rossi Agnessi Pebriana Sakai, Kenji Salma, Isni Salma Salsabillah Salsabila , Nida Ayu Sanomoto, Kenji Saputri, Ocha Sa’roni, Achmad Septianti, Kiki Setiawan, Arinda Sabrina Shabrina, Nadhilah Shofiah , Laila Nur Sholeha, Irdatus Sonya Hakim Raharjo Subekhi, Jovenlee Gibran Susanti, Eka Puji Susilowati Susilowati Syaifullah, Rangga Yudha Tarigan, Eigiya Nina Tashiro, Yukihiro Tri Elok Setya Megasari Udroto, Firsta Retnaningtyas Ulfiani, Khoirun Nisya' Uzinuzulla, Yunita Virda Wardani, Salza Belila Kusuma Wardhani, Maharani Tri Wibowo, Jihan Nafila Widarti, Rr Diva Naila Putri Wiwik Pratiwi Yahya, Ahmad Daily Yohanes Yohanes Yohanes Yolanda Putri, Ditta Kharisma Yolanda, Ditta Kharisma Yukihiro Tashiro Yukti Nurani Yukti Nurani Zuhriah Mumtazah