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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pengajaran MIPA Jurnal Pendidikan Indonesia Formica Education Online Journal of Mathematical and Fundamental Sciences Jurnal Pendidikan Biologi Indonesia Jurnal Penelitian dan Pembelajaran IPA Science and Technology Indonesia Jurnal Penelitian Pendidikan IPA (JPPIPA) Pedagonal : Jurnal Ilmiah Pendidikan Bioscientist : Jurnal Ilmiah Biologi These proceedings represent the work of researchers participating in The International Conference on Elementary Education (ICEE) which is being hosted by the Elementary Education Study Programme School of Postgraduate Studies, Universitas Pendidikan Proceeding Biology Education Conference Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Applied Environmental Science Asian Journal of Toxicology, Environmental, and Occupational Health Jurnal Bisnis Kehutanan dan Lingkungan Jurnal Inovasi Pangan dan Gizi Jurnal Pendidikan MIPA Sasambo: Jurnal Abdimas (Journal of Community Service)
Didik Priyandoko
Universitas Pendidikan Indonesia
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : HAYATI Journal of Biosciences

 1 
Molecular Phylogenetic Screening of Withania somnifera Relative From Indonesia Based on Internal Transcribed Spacer Region Topik Hidayati; Didik Priyandoko; Putri Yunitha Wardiny; Dina Karina Islami
HAYATI Journal of Biosciences Vol. 23 No. 2 (2016): April 2016
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.581 KB) | DOI: 10.4308/hjb.23.2.92

Abstract

Withania somnifera (family Solanaceae), known commonly as Ashwaganda, is one of the important medicinal plants, and recent studies reported that Withanone, one of the chemical components in this plant, has ability to kill cancer cell. Because of endemic state of this plant to South Asia, exploring plant species under the same family which grow well in Indonesia has been of interest. The purpose of this study was to screen the Indonesian plant which has strong phylogenetic relationship with Ashwaganda. Thus, phylogenetic analysis using DNA sequences of internal transcribed spacer (ITS) region was conducted. Thus, 19 species of Solanaceae and two species of Convolvulaceae as outgroup were examined. Five ITS regions of Ashwaganda retrieved from GenBank were included in the phylogenetic analysis. Parsimony analysis showed that Indonesia Solanaceae comprises seven groups which is consistent with the global Solanaceae relationship as previously reported. Furthermore, our study revealed that two species, Physalis angulata and Physalis peruviana, are relative to W. somnifera. Morphologically, they share characters of flower and fruit. This result indicated that these two species are potential to have similar chemical properties as Ashwaganda, thus we can have new variants of Withanone originated from Indonesia with similar effect.
Successful Primer Picking and Pooling for the Design of Multiplex PCR Primers Specific to Pork, Beef, Chicken, and Rat DNA Kusumawaty, Diah; Faridah, Nurul; Fibriani, Azzania; Priyandoko, Didik; Dzikrina, Hanina; Puspitasari, Diah; Tallei, Trina Ekawati; Aryani, Any
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.678-686

Abstract

DNA markers and Multiplex-PCR have emerged as methods for species detection in processed meat products. The primary objective of this study is to design multiplex primer sequences for pork, rat, beef, and chicken, generating distinguishable amplicons through agarose gel electrophoresis for halal detection in processed meat products. Primer design involved utilizing mitochondrial genomic data and the NCBI-Primer BLAST site to obtain specific pork and beef primer sequences. In silico simulations, including single and multiplex-PCR, were conducted using Primer Pooler. In vitro validation encompassed Single-PCR and Multiplex-PCR annealing temperature optimization, using samples of chicken, beef, pork, and rat as well as processed meat products like meatballs, sausages, and nuggets. In vitro validation demonstrated that the halal marker gene's multiplex primer efficiently amplified the target sequence, specifically at the optimal annealing temperature of 58°C. Amplicons from beef (1,217 bp), pork (860 bp), rat (622 bp), and chicken (272 bp) primers could be distinguished on a 1.5% agarose gel. The study's results can aid in cost-effective and rapid halal testing and authentication of processed meat products, offering advantages over PCR with a single primer.
Co-Authors Adi Rahmat Agustin, Dhini Ahmad Faried Alexandro, Garry Alyasa, Rahadian Rasyid Anisa Noor Aulia Annaba, Aziz Any Aryani Azis, Rizal Azmi, Haibah Azzania Fibriani Bangun, Adityana Arta Dadang Machmudin Deocaris, Custer C. Diah - Kusumawaty Diah Puspitasari Dina Karina Islami Dzikrina, Hanina Eka Yudha Rahman Eliya Mei Sisri Faidah, Siti Tahany Rifa Fauziyah, Lathifah Zahra Febblina Daryanes Fitriani Nurpratiwi Susanto Fransisca Sudargo Gina Angelina Hadiprasetyo, Dhanar Septyawan Handayanie, Yunni Hanna Sari Widya Kusuma Harry Murti Hernawati Hernawati Hodijah, Siti Romlah Noer Janitra, Fikrah Jeffrey ., Jeffrey Juansah, Rahadian Deden Kartika Kaul, Sunil C. Kusdianti Kusdianti Kusdianti Leonita, Lastri Mamat Arohman Mrs Sariwulan Diana Muzakki, Naufal Ahmad Nainggolan, Ita Margaretha Nashi Widodo Naufal Ahmad Muzakki Naufal Ahmad Muzakki Naufal Ahmad Muzakki Nia Kania Nia Nurdiani Norine, Jennifer Nurul Faridah, Nurul Nuryani Rustaman Putri Yunitha Wardiny Qlintang, Sandy Rahmat, Adi RAHMAT, DENI Rahmi, Annisa Salsyabila Rizal Rizal S. Saefudin Sa'diatul Fuadiyah Sabrina, Adilah Hafizha Nur Saefudin Saefudin Sajidah, Salmaa Nabiilah Santoso, Fadhila Zahra Sari, Prima Mutia Sholihah, Ika Adhani Sholihah, Uswatun Siti Sriyati Suhara, Nur Fajriyah Surtikanti Hertien Koosbandiah, Surtikanti Sylva Sagita Teresa Liliana Wargasetia Topik Hidayat Triharsiwi, Dwi Nur TRINA EKAWATI TALLEI Try Kurniawan Vinna Kurniawati Sugiaman, Vinna Kurniawati Wadhwa, Renu Wahyu Surakusumah Wahyu Widowati WAHYU WIDOWATI Wahyudin, Wahyudin Wawan Setiawan Wawan Setiawan Yayan Sanjaya Yunita, Shella Yusuf Hilmi Adisendjaja Zahira, Nurul Zahra Zahra, Cheryl Dwi Nur