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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pengajaran MIPA Jurnal Pendidikan Indonesia Formica Education Online Journal of Mathematical and Fundamental Sciences Jurnal Pendidikan Biologi Indonesia Jurnal Penelitian dan Pembelajaran IPA Science and Technology Indonesia Jurnal Penelitian Pendidikan IPA (JPPIPA) Pedagonal : Jurnal Ilmiah Pendidikan Bioscientist : Jurnal Ilmiah Biologi These proceedings represent the work of researchers participating in The International Conference on Elementary Education (ICEE) which is being hosted by the Elementary Education Study Programme School of Postgraduate Studies, Universitas Pendidikan Proceeding Biology Education Conference Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Applied Environmental Science Asian Journal of Toxicology, Environmental, and Occupational Health Jurnal Bisnis Kehutanan dan Lingkungan Jurnal Inovasi Pangan dan Gizi Jurnal Pendidikan MIPA Sasambo: Jurnal Abdimas (Journal of Community Service)
Didik Priyandoko
Universitas Pendidikan Indonesia
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : Science and Technology Indonesia

 1 
The Potential of Ethanol Extract of Pasak Bumi Roots (Eurycoma longifolia Jack) as an Anti-Prostate Cancer In Vitro Against PC-3 Cells Kania, Nia; Rahman, Eka Yudha; Priyandoko, Didik; Sabrina, Adilah Hafizha Nur; Widowati, Wahyu; Azis, Rizal; Annaba, Aziz; Hadiprasetyo, Dhanar Septyawan; Alexandro, Garry
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.452-466

Abstract

The prevalence of prostate cancer cases in men is expected to continue increase. In 2040, it is estimated that there will be 2.293.818 new cases and a 1.05% increase in the death rate due to prostate cancer. Eurycoma longifolia Jack roots extract (ELE) has potential as an alternative treatment. This study aims to analyze ELE potential as an anti-prostate cancer agent through in silico assay and in vitro assays on the prostate cancer cell line (PC-3). ELE compounds were docked against Casp-3, Casp-8, HAX-1, p27, and PTEN. In vitro assays on PC-3 cells were used, namely cell viability (WST-8), ROS levels; cell cycle; and cell apoptosis (flow cytometry), PC-3 cell senescence (μ-Galactosidase staining), Casp-3; Casp-8; HAX-1; p27; and PTEN gene expression (qRT-PCR). All proteins target were successfully docked with ELE compounds and presented binding interactions. ELE is known to reduce viability, intracellular ROS levels, live cells, necrosis, and reduce HAX-1 gene expression, and inhibit the cell cycle G0/G1 phase. ELE can also increase inhibition, senescence, late and early apoptosis, and Casp-3, Casp-8, p27, and PTEN gene expression. ELE 100 μg/mL is the most effective concentration. ELE has potential as an anti-prostate cancer agent through apoptosis, cell cycle, and antioxidant pathways
Potential of Secretome Hydrogel for Wound Healing in LPS- and Scratch-Induced BJ Cells as an Inflammation Model Widowati, Wahyu; Rahmat, Deni; Faried, Ahmad; Nainggolan, Ita Margaretha; Priyandoko, Didik; Wargasetia, Teresa Liliana; Sugiaman, Vinna Kurniawati; Triharsiwi, Dwi Nur; Qlintang, Sandy; Murti, Harry; Azis, Rizal; Jeffrey, Jeffrey
Science and Technology Indonesia Vol. 10 No. 4 (2025): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.4.1242-1254

Abstract

Wound healing often requires specialized interventions to accelerate recovery due to prolonged inflammation and limited regenerative factors. Umbilical Cord Mesenchymal Stem Cells secretome (UCMSCs) comprises various cytokines and growth factors that can promote wound healing. This study aims to analyze the potential of a secretome-based hydrogel as a wound-healing agent using BJ fibroblast cells induced by lipopolysaccharide (LPS) and scratch injury as an inflammation model. The secretome hydrogel was formulated using Carbopol, Hydroxypropyl Methylcellulose (HPMC), Hydroxyethyl Cellulose (HEC), and secretome. Cytotoxicity was conducted using the WST-8 assay, while cell migration was evaluated through a scratch assay. Tumor Necrosis Factor-???? (TNF-????), Nuclear Factor kappa-B (NF-????B), and Interleukin-8 (IL-8) gene expression were analyzed via qRT-PCR. Additionally, malondialdehyde (MDA) levels were measured for oxidative stress assessment, whereas Connective Tissue Growth Factor (CTGF) and Transforming Growth Factor-????1 (TGF-????1) levels were quantified using ELISA and colorimetric assays. The secretome hydrogel exhibited no cytotoxic effects on BJ fibroblast cells and significantly enhanced cell migration. Moreover, it reduced the TNF-????, IL-8, and NF-????B expression, indicating anti-inflammatory activity. The hydrogel also decreased MDA levels while increasing TGF-β1 and CTGF expression, suggesting antioxidant properties and enhanced tissue regeneration in the inflammatory model. The secretome-based hydrogel presents a promising therapeutic approach for promoting chronic wound healing by modulating inflammation, reducing oxidative stress, enhancing tissue regeneration, and stimulating fibroblast migration.
Co-Authors Adi Rahmat Agustin, Dhini Ahmad Faried Alexandro, Garry Alyasa, Rahadian Rasyid Anisa Noor Aulia Annaba, Aziz Any Aryani Azis, Rizal Azmi, Haibah Azzania Fibriani Bangun, Adityana Arta Dadang Machmudin Deocaris, Custer C. Diah - Kusumawaty Diah Puspitasari Dina Karina Islami Dzikrina, Hanina Eka Yudha Rahman Eliya Mei Sisri Faidah, Siti Tahany Rifa Fauziyah, Lathifah Zahra Febblina Daryanes Fitriani Nurpratiwi Susanto Fransisca Sudargo Gina Angelina Hadiprasetyo, Dhanar Septyawan Handayanie, Yunni Hanna Sari Widya Kusuma Harry Murti Hernawati Hernawati Hodijah, Siti Romlah Noer Janitra, Fikrah Jeffrey ., Jeffrey Juansah, Rahadian Deden Kartika Kaul, Sunil C. Kusdianti Kusdianti Kusdianti Leonita, Lastri Mamat Arohman Mrs Sariwulan Diana Muzakki, Naufal Ahmad Nainggolan, Ita Margaretha Nashi Widodo Naufal Ahmad Muzakki Naufal Ahmad Muzakki Naufal Ahmad Muzakki Nia Kania Nia Nurdiani Norine, Jennifer Nurul Faridah, Nurul Nuryani Rustaman Putri Yunitha Wardiny Qlintang, Sandy Rahmat, Adi RAHMAT, DENI Rahmi, Annisa Salsyabila Rizal Rizal S. Saefudin Sa'diatul Fuadiyah Sabrina, Adilah Hafizha Nur Saefudin Saefudin Sajidah, Salmaa Nabiilah Santoso, Fadhila Zahra Sari, Prima Mutia Sholihah, Ika Adhani Sholihah, Uswatun Siti Sriyati Suhara, Nur Fajriyah Surtikanti Hertien Koosbandiah, Surtikanti Sylva Sagita Teresa Liliana Wargasetia Topik Hidayat Triharsiwi, Dwi Nur TRINA EKAWATI TALLEI Try Kurniawan Vinna Kurniawati Sugiaman, Vinna Kurniawati Wadhwa, Renu Wahyu Surakusumah Wahyu Widowati WAHYU WIDOWATI Wahyudin, Wahyudin Wawan Setiawan Wawan Setiawan Yayan Sanjaya Yunita, Shella Yusuf Hilmi Adisendjaja Zahira, Nurul Zahra Zahra, Cheryl Dwi Nur