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Surahmaida, Surahmaida
Akademi Farmasi Surabaya
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Journal : Biospecies

 1 
Uji Daya Hambat Ekstrak Metanol Auricularia nigricans terhadap Candida spp.: Inhibitory Test of Methanol Extract of Auricularia nigricans Against Candida spp. Indrawati, Afina; Pertiwi, Amelya Dyah; Ayuningtyias, Audry Ridho; Subroto, Hillda Widianingtyas; Azizah, Mufidhatul Nur; Handayani, Tutik; Surahmaida, Surahmaida; Lestari, Kinanti Ayu Puji; Yuliarni, Floreta Fiska
Biospecies Vol. 16 No. 2 (2023): Juli 2023
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v16i2.27451

Abstract

Candida was a fungus that can cause candidiasis infection. Candidiasis could occur of the skin, nails, mucous membranes and the gastrointestinal. The study aims to examine whether Auricularia nigricans methanol extract has the ability to inhibit the growth of Candida albicans, C. glabrata, and C. parapsilosis. Soxhletation was used as the extraction method, and the inhibitory test method involved diffusion paper disk and wells methods. The concentration variations used were 0.2 g/ml, 0.25 g/ml and 0.3 g/ml with 10% DMSO as a negative control. The research results obtained were that no inhibition zones were formed at concentrations of 0.2 g/ml, 0.25 g/ml and 0.3 g/ml so that they were included in the inactive category. Keywords: Auricularia nigricans, Candida, soxhlet method, methanol extract, paper disc and wells method.   Abstrak Candida merupakan jamur golongan khamir yang dapat menyebabkan infeksi kandidiasis. Kandidiasis tersebut dapat terjadi pada kulit, kuku, membran mukosa dan saluran cerna. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak metanol Auricularia nigricans dalam menghambat pertumbuhan C. albicans, C. glabrata dan C. parapsilosis. Metode ekstraksi yang digunakan adalah sokletasi selama 10 jam dan metode uji daya hambat antifunginya secara difusi cakram dan sumuran. Variasi konsentrasi yang digunakan adalah 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml dengan DMSO 10% sebagai kontrol negatif. Hasil penelitian yang diperoleh adalah tidak terbentuknya zona hambat pada konsentrasi 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml sehingga termasuk dalam kategori tidak aktif. Kata kunci: Auricularia nigricans, Candida, metode sokletasi, ekstrak metanol, metode difusi cakram dan sumuran.
Brine Shrimp Lethality Test (BSLT) of 96% Ethanol Extract of Ande-ande Lumut (Selaginella doederleineii) Leaves Difa Prasticha, Yunita; Surahmaida
Biospecies Vol. 17 No. 2 (2024): Juli 2024
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v17i2.35582

Abstract

Selaginella doederleineii is a fern plant that is efficacious for treating coughs, pneumonia and broken bones. Leaves are the primary medicinal parts. However, there has been no research regarding the toxicity of Selaginella doederleineii leaves. This study aims to evaluate potential toxicity of 96% ethanol extract of Selaginella doederleineii leaves against shrimp larvae (Artemia salina Leach) using the Brine Shrimp Lethality Test (BSLT) method. The toxicity value is shown by the LC50 value. This research method includes the extraction stage, larvae hatching stage, stock solutions and concentration dilutions of the extract preparation, toxicity test and LC50 value calculation. The toxicity test using 10 Artemia salina shrimp larvae were exposed to each test concentration (0 ppm, 1 ppm, 2 ppm, 3 ppm, 4 ppm, 5 ppm) in triplicate. Larval mortality was assessed after 24 hours to calculate mortality percentage and LC50. The results showed that the LC50 value for the 96% ethanol extract of Selaginella doederleineii was 12.0221 ppm, indicating very toxic. It can be concluded that Selaginella doederleineii leaves exhibits significat toxicity to brine shrimp and potential to be developed as a raw material for anti-cancer drugs. 
UJI ANGKA LEMPENG TOTAL DAN ANGKA KAPANG KHAMIR PADA Pleurotus ostreatus DAN Ganoderma lucidum Putri, Amanda Nabila; Setiawati, Asti Anwar; Masruuroh, Dina; Absani, Afriyanti Rifqi; Surahmaida, Surahmaida; Lestari, Kinanti Ayu Puji; Yuliarni, Floreta Fiska
Biospecies Vol. 18 No. 1 (2025): Januari 2025
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v18i1.36424

Abstract

Mushrooms are a source of antioxidants. Mushrooms can be processed into various products. Both raw materials and finished products from mushrooms must comply with established standards. The aim of this research was to determine the total plate count and total fungal count in Ganoderma lucidum and Pleurotus ostreatus samples. The method used is the Total Plate Count (TPC) and Total Fungal Count (TFC). The TPC value in G. lucidum was 6.4 x 106 CFU/g and TFC was 3.9 x 105 CFU/g. This value exceeds the BPOM maximum standard for tea products or herbal brews. The TPC value in P. ostreatus was 1.2 x 105 CFU/g and TFC was 4.9 x 105 CFU/g. The TPC value for P. ostreatus is less than the maximum standard set by BPOM, while the TFC value exceeds the maximum standard for powder or mixture of soup and broth. The conclusion of this research is that the TPC and TFC in the G. lucidum and P. ostreatus samples exceed the standards set by BPOM No. 13 of 2019 except for the TPC in the P. ostreatus sample.
Co-Authors ., Syukrianto Absani, Afriyanti Rifqi Aisa, Iis Andi Shifa Nur Aynun Anisa Rizki Amalia Arien Puspa Retno Aristyawan, Andhika Dwi Ayuningtyias, Audry Ridho Azizah, Mufidhatul Nur Bogar, Aurelia Nabila Salsa Putri Cicik Herlina Yulianti Cicik Herlina Yulianti Cicik Herlina Yulianti Dewi, Novicalia Citra Difa Prasticha, Yunita Febriana, Eryka Ayu Firdausyiah, Ina Floreta Fiska Yuliarni Galuh Gondo Kusumo Handayani, Elia Handrianto, Prasetyo Indrawati, Afina Junairiah Junairiah Kaseh, Chandra Cladio Kinanti Ayu Puji Lestari Lilis Sulistyorini Lisanti, Istiqomah Dwi Wahyu M.A. Hanny Ferry Fernanda Masruuroh, Dina Mercyska Suryandari Meyke Herina Syafitri Mochammad Sulton Aziz Irawan Ni'matuzahroh Ni'matuzahroh Nisrina Alifah Khairunnisa Pertiwi, Amelya Dyah Putri, Amanda Nabila Rachmah, Artifa Rachmawati, Amelinda Rahmadhany, Wulan Desy Rani, Agustin Widia Rio Alta Rio Novendra Rizka Surya Ningrum Rizky Darmawan, Rizky Sa’diyah, Lailatus Sandi Al Afif Fathoroni Putra Sarwoko Mangkoedihardjo Setiawati, Asti Anwar Siti Nurhaliza Sofiana, Dewi Amelia Sri Nurhatika Sri Nurhatika Subroto, Hillda Widianingtyas Suryadari, Mercyska Syukrianto Syukrianto Tri Puji Lestari Sudarwati Tutik Handayani, Tutik Ulfa, Ninik Mas Umar Umar Umar, Umarudin Umarudin Umarudin, Umarudin Wulansari, Silvi ayu Yosephine Sri Wulan Manuhara Yuliarni, Floreta Fiska Zahra, Zulia