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All Journal Jurnal Riset Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JPP Jurnal Kesehatan Poltekkes Palembang Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) COMMUNITY : Jurnal Pengabdian Kepada Masyarakat International Journal of Advanced Health Science and Technology JHeS (Journal of Health Studies) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Pengabdian Kesehatan Beguai Jejama Frontiers in Community Service and Empowerment Health Dynamics Jurnal Teknokes
Museyaroh, Museyaroh
Department Of Medical Laboratory Technology, Health Polytechnic Ministry Of Health Surabaya, Surabaya, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Health Dynamics

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The Relationship Between TSH and Indirect Bilirubin Levels in Neonates Suspected of Having Jaundice Alizah, Nur; Handayati, Anik; Museyaroh, Museyaroh; Suhariyadi, Suhariyadi
Health Dynamics Vol 1, No 10 (2024): October 2024
Publisher : Knowledge Dynamics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hd11006

Abstract

Background: Congenital hypothyroidism (CH) is a condition of thyroid hormone deficiency that occurs at birth. The TSH (thyroid-stimulating hormone) test is crucial for diagnosing hypothyroidism. CH is known to cause prolonged unconjugated hyperbilirubinemia. Therefore, this study aimed to analyze the relationship between TSH and indirect bilirubin levels in neonates suspected of having jaundice. Methods: This is a non-experimental, retrospective study conducted at Lombok Dua Dua Lontar Mother and Child Hospital in Surabaya. The study involved data collection on neonates aged 2–7 days suspected of jaundice, whose TSH and indirect bilirubin levels were measured between November 2022 to April 2024. Results: Among 100 neonates, 62% were aged 2-4 days, while 38% were aged 5-7 days. The majority were male (56%), with female comprising 44%. Of the 100 neonates, only 1 (1%) had borderline TSH levels, while 99% had normal TSH levels. Hyperbilirubinemia was observed in 94% of the neonates, while 6% had normal indirect bilirubin levels. Statistical analysis using the Spearman correlation showed no significant link between TSH and indirect bilirubin levels (p = 0.802). Conclusions: While this study did not find a clear connection between TSH and indirect bilirubin levels in neonates suspected of having jaundice, one case of borderline TSH was identified. This neonate required referral to pediatric endocrinology, as untreated congenital hypothyroidism can lead to mental retardation. Despite limited research linking TSH and bilirubin levels in jaundiced neonates, routine screening for congenital hypothyroidism using TSH testing should be reconsidered. Future studies could benefit from focusing on specific causes of neonatal jaundice to help narrow down research questions in this area.
Analysis of the Leukocyte Profile for Peripheral Blood Smear Stained with Diff-Count Based on Fixation Time Variation Museyaroh, Museyaroh; Nabilah, Musholli Himmatun; Endarini, Lully Hanni
Health Dynamics Vol 2, No 2 (2025): February 2025
Publisher : Knowledge Dynamics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hd20202

Abstract

Background: Peripheral blood smear examination (HDT) is a test to observe the morphology of blood cells microscopically. A peripheral blood smear is a simple method, and its examination is widely available in laboratories. The advantage of peripheral blood smear examination is its ability to assess various components of peripheral blood cells, such as cell morphology (erythrocytes, leukocytes, and platelets). One of the commonly used methods for peripheral blood smear examination is Giemsa staining. The process of Giemsa staining fixation aims to preserve the cell structure and its components before the staining is performed. The precise fixation time is key to obtaining optimal staining results, which allows for accurate observation of cell morphology. The aim of this study is to analyze the morphology of leukocyte profiles in peripheral blood smears stained with Giemsa based on variations in fixation time to determine the optimal time. Methods: This research was conducted in August-September 2024. The sample in this study was whole blood taken from the academic community of the Department of Medical Laboratory Technology and examined with a peripheral blood smear using Giemsa staining, and analyzed using descriptive statistical tests with the SPSS for Windows 22 software. Results: The research results showed that at a fixation time of 3 minutes, 60% were good and 40% were less good; at 5 minutes, 100% were good; at 10 minutes, 100% were good; at 15 minutes, 40% were good and 60% were less good; and at 20 minutes, 20% were good and 80% were less good. Conclusion: The best time variation in Giemsa staining using phosphate buffer is fixation for 5 minutes and 10 minutes, where all preparations (5 preparations) are 100% in the good category.
Co-Authors ., Pestariati Alizah, Nur Amalia Putri Khurota Ayyun Amania, Salsabila Anita Dwi Anggraini Aryati Aryati Aryati Aryati Briansky, Sabira Alvayogi Deddy Adam Diah Titik Mutiarawati Diah Titik Mutiarawati Dwi Kriharyani Edy Haryanto Edy Haryanto Endarini, Lully Hanni Era Fitria Yunita Ersanto, Novi Evi Yunita Nugrahini Evy Diah Woelansari Evy Dyah Woelansari Farah Adelia Qotrunnada Ginarsih, Yuni Handayati, Anik Haqqi, Faishol Hermiyanti, Pratiwi Hindaryani, Nurul Ida Ariyanti, Ida Ilma Ainun Nisa Ira Puspita Sari Ira Rahayu Tiyar Isnaini, Dian Istanto, Wisnu Juliana Christyaningsih Kasiati Kiaonarn Ongko Waluyo Kiaonarni O.W Kristiningsih, Elly Kuswanto, Muhammad Laili Rahmawati, Laili Lembunai Tat Alberta Liliek Soetjiatie Lully Hani Endarini Lully Hani Endarini Luthfi Rusyadi M. Zakarahman Makruf, Anang Mamik Mamik Mamik Mamik Masdiva Putri Hidayah Maulida Azmi Wimasni Inah Minarti Minarti Minarti Minarti Mujayanto Musholli Himmatun Nabilah Mutiarawati, Diah Titik Nabilah, Musholli Himmatun Novikasari, Caturizkyana Novita, Devy Nuning Marina Pengge Nur Hatijah Nur Kholifah, Siti Nurul Hindaryani Nurwening TW P, Teresia Retna Puspa Wardani Puspa Wardhani Puspitasari, Erlita Rahayu Sumaningsih Rahmania, Adelia Prisma Retno Puspitadewi, Teresia Retno Sasongkowati Retno Sasongkowati Retno Werdiningsih Rusmania, Liya Safrudin, Samsul Saktiyani, Fisari Sasongkowati, Retno Sri Sulami Endah Astuti Sri Utami Sugihani Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Sumasto, Hery Suyanto, Beny Teta Puji Rahayu Toni Watoyani Trisnania, Iid Dani Wisnu Istanto Yohanes Kambaru W