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PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN Takdir Saili; I Ketut Mudite Adnyana; Ronny Rachman Noor; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Several methods i.e. cooling, freezing, and freeze-drying have been widely used to preserve spermatozoa with various degree of success. Freeze-drying appears to provide a method to preserve spermatozoa in a dry state without requiring liquid nitrogen for storing frozen spermatozoa. Freeze-drying procedures can have a detrimental effect on plasma membrane and acrosomal cap of the spermatozoa. In this experiment study, the viability and subcellular changes of freeze-dried ram spermatozoa were evaluated using staining method and scanning electron microscopy. The results revealed that all freeze-dried spermatozoa were dead following evaluation using eosin staining and Hoechst-propidium iodide staining methods. Morover, plasma membrane and acrosomal cap of freeze-dried ram spermatozoa was disrupted observed using scanning electron microscope.

Karakteristik Morfologi, Perbedaan Jenis Kelamin, dan Pendugaan Umur Burung Weris (Gallirallus philippensis) di Minahasa, Sulawesi Utara (MORPHOLOGICAL CHARACTERISTICS, SEX DIFFERENCES, AND AGE ESTIMATION OF WERIS (GALLIRALLUS PHILIPPENSIS) FROM MINAHASA Lucia Johana Lambey; Ronny Rachman Noor; Wasmen Manalu; Dedy Duryadi
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This study aim to observeb Gallirallus philippensis similarities between different sites in Minahasa,to detect sexual dimorphism, and to estimate the bird age based on morphological characteristics. A totalof 35 birds were collected from four locations i.e. Tondano, Papontolan, Ranoyapo, and Wusa in MinahasaNorth Sulawesi. The result showed that there were no differences in the length of beak, wing, and shankbetween birds from the different locations. Moreover, based on the Principle Component Analysis (PCA)the bird’s shank and tail’s length were the main component. Based on cluster analysis, the birds from fourlocations had a close similarities. The male bird’s body weight, beak length, and beak width were largercomponent to the female’s. The bird’s age could be best predicted by their iris colour, beak colour, feather colourof the neck and the growth of the wing feathers, respectively.

Kurva Pertumbuhan Sapi Friesian Holstein dari Lahir Sampai Siap Kawin Berdasarkan Tingkat Kelahiran (HOLSTEIN GROWTH CURVE OF NEW BORN CALF UNTIL FIRST MATING BASED ON BIRTH RATE) Lia Budimulyati Salman; Cece Sumantri; Ronny Rachman Noor; Asep Saefuddin; Chalid Talib
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The growth curve is a reflection of the ability of an individual or population to actualize themselves aswell as the size will be the development of the parts of the body until it reaches the maximum size (adult)on the existing environmental conditions. The main goal of this study is to determine the growth curvemodel of Friesian Holstein cattle from birth until ready to mate based on birth rates with Logistic model,Gompertz, and von Bertalanffy. The data was used in this study is the result of the weighing of dairy cowsbody weight of female Friesian Holstein as many as 335 head number collected by PT Taurus Dairy FarmSukabumi from 2001to 2011, which have complete data from birth until first mating with a birth rate thatis different from the birth of the one to five. The data used in the analysis of growth curves using three nonlineargrowth curve model i.e. Logistic models, Gompertz, and Von Bertalanffy. The results obtainedindicate that the growth curve model shave different levels of accuracy, depending on the environment andage or duration of observation, the longer observed to mature weight (A), the age of puberty, and pubertygreater weight. The Conclusion of this study is a model of Logistic close to field conditions. Morover thehigher the birth rate will be heavier birth weight and adult weight. These three non-linear mathematicalmodel used in this study has a high degree of accuracy.

PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN Takdir Saili; I Ketut Mudite Adnyana; Ronny Rachman Noor; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Several methods i.e. cooling, freezing, and freeze-drying have been widely used to preserve spermatozoa with various degree of success. Freeze-drying appears to provide a method to preserve spermatozoa in a dry state without requiring liquid nitrogen for storing frozen spermatozoa. Freeze-drying procedures can have a detrimental effect on plasma membrane and acrosomal cap of the spermatozoa. In this experiment study, the viability and subcellular changes of freeze-dried ram spermatozoa were evaluated using staining method and scanning electron microscopy. The results revealed that all freeze-dried spermatozoa were dead following evaluation using eosin staining and Hoechst-propidium iodide staining methods. Morover, plasma membrane and acrosomal cap of freeze-dried ram spermatozoa was disrupted observed using scanning electron microscope.

Keragaman Gen Heat Shock Protein 70 pada Ayam Kampung, Ayam Arab, dan Ayam Ras (POLYMORPHISMS OF THE HEAT SHOCK PROTEIN 70 GENE IN KAMPONG, ARABIC, AND COMMERCIAL CHICKENS) Mohamad Hasil Tamzil; Ronny Rachman Noor; Wasmen Manalu; Cece Sumantri
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of the research was to identify the polymorphisms of HSP-70 gene of kampong, Arabic, andcommercial chickens. The study used whole blood samples taken from the branchial vein at the age of 12weeks from a group of chickens that were kept since DOC. Kampong chickens DOC were obtained byhatching the eggs that were collected from lowlands, medium and high altitude areas in Lombok island,while the Arabic chickens DOC were obtained from farmer. DOC of commercial chickens were purchased atthe poultry shop. The genotyping results using PCR-SSCP analysis showed that kampong and arabicchickens were polimorphic, while commercial chickens were classified as monomorphic. Kampong chickenshad seven genotypes of HSP-70, namely AA, AB, AC, CC, AD, DD, and BC. The AD genotypes had thehighest frequency, whereas in arabic chickens had six genotypes, namely AA, AB, AC, CC, AD and BC,which AC genotype had the highest frequencies. However, the commercial chickens contain only onegenotype which was DD. There were three polymorphic sites found in amplificated area, namely AÆGmutation at site 617, AÆG mutation at site 628, GÆC mutation at site 646, CÆT mutation at site 661,AÆG mutation at site 699, GÆC mutation at site 754, and AÆG mutation at site 792. Mutations at sites628, 646 and 661 were considered as silent mutation. HSP-70 gene in kampung and arabic chickenpopulation in this study was in equilibrium. The value of Ho, He, and PIC of kampung and arabic chickenswere not different.

Performance of the third generation striped catfish, Pangasianodon hypophthalmus Sauvage, 1878 as results of the selection for bodyweight character in Freshwater Aquaculture Fisheries Center, Sungai Gelam, Jambi Irwan Irwan; Dinar Tri Soelistyowati; Odang Carman; Ronny Rachman Noor
Jurnal Iktiologi Indonesia Vol 19 No 3 (2019): October 2019
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v19i3.469

Selective breeding of striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) has been conducted at BPBAT Sungai Gelam to produce a growth line with mass selection method. Until 2018, the selective breeding program has produced three generations. Therefore, it was necessary to evaluate the performance of the third generation growth line (G3Ps) at the reproductive, seed and grow-out phase compared to the second generation the base population (G2Ds). A total of 10 pairs of broodstock (G3Ps and G2Ds) were spawned then the seeds were raised for 120 days in the media with regular water exchange (first treatment: T1) and without water exchange (second treatment: T2). The results showed that the number of eggs per gram of G3Ps (1600±124 eggs g-1) was significantly different from G2Ds (1490±101 eggs g-1). The bodyweight of G3Ps seeds at aged 40 days larger than G2Ds with selection response is 32.25%, while survival and feed efficiency were not significantly different. At the grow-out phase, between treat-ments were not significantly different and there was no interaction between lines and treatments for all the characters measured (p>0.05). The bodyweight of G3Ps was larger than the G2Ds with response selection 18.41% in T1 and 42.6% in T2. The control used was the base population so that the selection response obtained was an accumulation of three generations. Thus the selection response per generation was 6.14% in T1 and 14.20% in T2 measured at 162 days from hatching. It can be concluded that there is an improvement in the character of bodyweight for the third generation of growth line (G3Ps) as results of the selection at BPBAT Sungai Gelam both in good (T1) and bad environment (T2). Abstrak Seleksi ikan patin siam (Pangasianodon hypophthalmus Sauvage, 1878) telah dilakukan di BPBAT Sungai Gelam untuk meningkatkan pertumbuhan dengan metode seleksi individu. Sampai tahun 2018, seleksi tersebut telah meng-hasilkan tiga generasi. Penelitian ini bertujuan untuk mengevaluasi performa generasi ketiga galur pertumbuhan (G3Ps) pada tahap reproduksi, pertumbuhan benih, dan ukuran konsumsi dibandingkan dengan populasi dasar gene-rasi kedua (G2Ds). Sebanyak 10 pasang induk G3Ps dan G2Ds dipijahkan kemudian benih yang dihasilkan dibesarkan selama 120 hari pada media dengan pergantian air secara berkala (perlakuan pertama: T1) dan tanpa pergantian air (perlakuan kedua: T2). Hasil penelitian menunjukkan bahwa jumlah telur per gram adalah 1600±124 butir pada G3Ps, berbeda nyata dengan G2Ds yaitu 1490±101 butir. Benih G3Ps umur 40 hari memiliki bobot tubuh lebih besar diban-dingkan G2Ds dengan respons seleksi sebesar 32,25%, sedangkan sintasan dan efisiensi pakan tidak berbeda nyata. Pada tahap pembesaran ukuran konsumsi, antar perlakuan tidak berbeda nyata dan tidak ada interaksi antargalur dan perlakuan untuk karakter bobot tubuh, panjang baku, sintasan dan efisiensi pakan (p>0,05). Karakter bobot tubuh G3Ps lebih besar dibandingkan dengan G2Ds dengan respons seleksi total untuk tiga generasi sebesar 18,41% pada T1 dan 42,6% pada T2. Dengan demikian respons seleksi per generasi sebesar 6,14% pada T1 dan 14,20% pada T2 yang diukur pada umur 162 hari dari menetas. Disimpulkan bahwa terjadi perbaikan pada karakter bobot tubuh untuk galur pertumbuhan generasi ketiga (G3Ps) hasil program seleksi di BPBAT Sungai Gelam baik pada lingkungan baik (T1) maupun lingkungan buruk (T2).

KELENTURAN FENOTIPIK UDANG GALAH (Macrobrachium rosenbergrr) STRAIN MUSI, BARITO , Glffiacro, DAN PERSILANGAN NYA PADA LINGKUNGAN BERSALINITAS Wartono Hadie; Komar Sumantadinata; Ronny Rachman Noor; Subandriyo Subandriyo; Odang Carman; Lies Emmawati Hadie
Jurnal Penelitian Perikanan Indonesia Vol 10, No 5 (2004): (Vol. 10 No. 5 2004)
Publisher : Pusat Riset Perikanan, BRSDM KP.

Penefitian kelenturan fenotipik udang galah (Macrobrachium rosenbergir) dilakukan pada salinitas 0%, 10%, dan '15%. Tiga strain yaitu Barito (BB), Musi(MM), dan GlMacro (GG) digunakanuntuk membentuk dan mengevaluasi populasi dasar tersebut pada salinitas yang berbeda. Perkawinan secara fullsib dan dialelle crosses dilakukan di dalam dan di antara strain. Larva hasil dari persilangan tersebut dipelihara pada bak kerucut dengan menggunakan sistem air jernih pada salinitas 12% - 15%. Pasca larva yang dihasilkan dari masing-masing persilangan dibesarkan di tambak pada tiga salinitas media yang berbeda selama lima bulan.

ANALISIS KEMAMPUAN DAYA GABUNG GEN PADA GENOTIPE UDANG GALAH UNTUK MENDUKUNG PROGRAM SELEKSI DAN HIBRIDISASI Wartono Hadie; Subandriyo Subandriyo; Lies Emmawati Hadie; Ronny Rachman Noor
Jurnal Penelitian Perikanan Indonesia Vol 11, No 5 (2005): (Vol. 11 No. 5 2005)
Publisher : Pusat Riset Perikanan, BRSDM KP.

Penelitian tentang daya gabung gen (conbining ability), dilakukan dengan mengunakan tiga strain udang galah yakni Musi, Barito, dan GlMacro. Perkawinan dilakukan secara fullsib di dalam dan di antara strain serta menghasilkan sembilan kombinasi genotipe.

Identifikasi Keragaman SNP Gen MSTN Pada Sapi Limousin Wenny Ladhunka Nur Aliyya; Jakaria Jakaria; Ronny Rachman Noor
Wahana Peternakan Vol. 6 No. 2 (2022): Wahana Peternakan
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v6i2.596

Abstract Limousin cattle (Bos Taurus) are beef cattle that have a large, long, body shape, with have advantages of fast body growth, high fertility and easy reproducibility. Myostatin gene is a family of TGF-β which plays a role in muscle growth and meat quality. This study aims to identify diversity of the Single Nucleotide Polymorphism (SNPs) Myostatine gene in the coding region in limousine cattle. Blood samples were taken from 15 limousine cattle from BPTU-HPT Padang Mangatas, West Sumatra Province. SNPs identification is finish by amplifying the coding region using the sequencing method. This study revealed 12 SNPs spread over 2 SNPs in exon 1 (c.400 G>A, c.415 C>A), 8 SNPs in exon 2 (c.2411 C>T, c.2477 G>A, c. .2489 G>A, c.2503 G>A, c.2521 G>A, c.2577 C>T, c.2609 G>A, c.2636 G>A), and in exon 3 find 2 SNPs (c .5107 C>A, c.5113 T>C) are polymorphic with allele frequencies in general being in Hardy-Weinberg equilibrium except for SNP c.2411 C>T which is not in balance. SNPs identified in the MSTN gene of limousine cattle are expected can fungtion as candidate genetic markers, especially for the perfection of beef cattle in Indonesia. Key words: Limousin Cattle, MSTN, SNP

Diversity of SNP c.795A>G PLAG1 Gene and its Association to Birth Weight of Bali Cattle Adefia Fahira; Ronny Rachman Noor; Jakaria Jakaria
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 3 (2022): September 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i3.3045

PLAG1 gene is one of those that regulate growth and body size. This study aimedto look at the PLAG1 gene polymorphism and its relationship to birth weight in Bali cattle using PCR-RFLP. The total sample used was 104 samples consisting of 66 Bali cattle from BPTU-HPT Denpasar and 38 Bali cattle from BPT-HMT Serading, each of which had birth weight data. PLAG1 gene polymorphism was analyzed using PCR-RFLP and the Sac1 restriction enzyme. The genotype and allele frequencies, heterozygosity, and Hardy-Weinberg equilibrium were all examined using Popgen32. General Linear Model was used to analyze the association of SNP 795AG PLAG1 gene with birth weight in Bali cattle. Amplification of the PLAG1 gene resulted in 776 bp fragments and two alleles. The PLAG1 gene had three genotypes: AA (562 bp and 182 bp), AG (562 bp, 182 bp, and 104 bp), and GG (562 bp, 182 bp, and 104 bp). Based on the results, the PLAG1 gene in Bali cattle was polymorphic. The alleles frequency of Bali cattle wasin Hardy-Weinberg equilibrium. The SNP c.795AG PLAG1 gene genotype were associated with birth weight in Bali cattle. The A allele is a determinant of high birth weight in Bali cattle where the AG genotype has the highest birth weight.

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