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All Journal Jurnal Ilmu Pertanian Indonesia Buletin Peternakan Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Animal Production : Indonesian Journal of Animal Production Informatika Pertanian Jurnal Penelitian Perikanan Indonesia Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Wahana Peternakan Jurnal Agripet Animal Production
Ronny Rachman Noor
Departemen Ilmu Produksi Dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor, Kampus IPB Darmaga Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Environmental Science Veterinary Other

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Polymorphism of CSN2 Gene Exon 7 in Indonesian Dairy Goat Breeds Gisela Deviandini; Ronny Rachman Noor; Epi Taufik; Santiananda Arta Asmarasari
Buletin Peternakan Vol 47, No 3 (2023): BULETIN PETERNAKAN VOL. 47 (3) AUGUST 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i3.84098

Abstract

Milk production and composition are the main factors in the dairy industry. However, these traits are influenced by several factors, such as genetic factors. The CSN2 gene exon 7 is one of the genes that can affect the protein composition of goat milk. Several studies on the CSN2 gene exon seven have been conducted on Italian and Indian goat breeds, and their genetic variations have been found. Studies about CSN2 gene exon seven polymorphisms in Indonesia have been completed but found no variations. Therefore, this study aims to find genetic variations of CSN2 gene exon 7 using the Sanger Sequencing Method. Ninety-five blood samples of 66 Sapera, 15 Saanen, and 14 Etawa Grade goats were collected from the Livestock Research Center, Ciawi. Genetic diversity was calculated using PopGene32 programs. The results found two polymorphic SNPs in all three dairy goat breeds, namely g.8946C>T and g.8956G>A. Three genotypes found in SNP g.8946C>T are CC, CT, and TT, while a new SNP g.8956G>A found two genotypes (GG and GA). In conclusion, SNPs g.8946C>T and g.8956G>A are polymorphic. Additional research should be conducted to determine whether there is any association between these 2 SNPs and milk production and quality.
Identifikasi Keragaman SNP Gen MSTN Pada Sapi Limousin Wenny Ladhunka Nur Aliyya; Jakaria Jakaria; Ronny Rachman Noor
Wahana Peternakan Vol. 6 No. 2 (2022): Wahana Peternakan
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v6i2.596

Abstract

Abstract Limousin cattle (Bos Taurus) are beef cattle that have a large, long, body shape, with have advantages of fast body growth, high fertility and easy reproducibility. Myostatin gene is a family of TGF-β which plays a role in muscle growth and meat quality. This study aims to identify diversity of the Single Nucleotide Polymorphism (SNPs) Myostatine gene in the coding region in limousine cattle. Blood samples were taken from 15 limousine cattle from BPTU-HPT Padang Mangatas, West Sumatra Province. SNPs identification is finish by amplifying the coding region using the sequencing method. This study revealed 12 SNPs spread over 2 SNPs in exon 1 (c.400 G>A, c.415 C>A), 8 SNPs in exon 2 (c.2411 C>T, c.2477 G>A, c. .2489 G>A, c.2503 G>A, c.2521 G>A, c.2577 C>T, c.2609 G>A, c.2636 G>A), and in exon 3 find 2 SNPs (c .5107 C>A, c.5113 T>C) are polymorphic with allele frequencies in general being in Hardy-Weinberg equilibrium except for SNP c.2411 C>T which is not in balance. SNPs identified in the MSTN gene of limousine cattle are expected can fungtion as candidate genetic markers, especially for the perfection of beef cattle in Indonesia. Key words: Limousin Cattle, MSTN, SNP
Polymorphism and Expression of HSD17β13 Gene and Its Association with Lamb Quality of Indonesian Sheep Ratna Sholatia Harahap; Ronny Rachman Noor; Asep Gunawan
ANIMAL PRODUCTION Vol. 23 No. 1 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.1.88

Abstract

HSD17β13 (17beta 13-Hydroxysteroid dehydrogenases) are important enzymes in steroid metabolism. This study investigated the polymorphisms and expression of the HSD17β13 gene in lamb quality traits in Indonesian sheep. A total of 200 of rams of seven breeds administered in the study were Javanese fat-tailed (JFT), Javanese thin-tailed (JTT), Garut sheep (GS), Jonggol sheep (JS), Garut composite sheep (GCS), Compass agrinac sheep (CAS), Barbados cross sheep (BCS) aged 10-12 months and weighed 20-30 kg. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and MspI enzyme restriction were performed to identify the polymorphism of the HSD17β13 gene. The lamb quality traits were measured for physical quality, flavor, odor, and fatty acid composition. The results showed that the HSD17β13 gene was polymorphic and had three genotypes i.e., CC, CT, and TT. The highest genotype frequency was the CC when compared to the other genotypes. The allele frequency of HSD17β13 gene was not in Hardy Weinberg Equilibrium. The polymorphism of HSD17β13 gene was significantly (P<0.05) associated with the fatty acid composition and Saturated Fatty Acid (SFA), i.e., tridecanoic acid (C13:0), arachidic acid (C20:0), henecosanoic acid (C21:0), and tricosanoic acid (C23:0). The polymorphism was also significantly associated with the Monounsaturated Fatty Acid (MUFA) i.e. ginkgoleic acid (C17:1) and nervonoic acid (C24:1), and Polyunsaturated Fatty Acid (PUFA) i.e. eicosedienoic acid (C20:2). The level of expression of the HSD17β13 gene based on quantitative real time-PCR analysis results was not significantly different (P>0.05) among genotypes for lamb quality traits. It can be concluded that the polymorphism of HSD17β13 gene was associated with the fatty acid composition of lamb in Indonesian sheep.
Polimorfisme Protein Darah Itik Pegagan dengan Metode PAGE Meisji Liana Sari; Ronny Rachman Noor; Peni S. Hardjosworo; Chairun Nisa
Jurnal Agripet Vol 11, No 2 (2011): Volume 11, No. 2, Oktober 2011
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v11i2.376

Abstract

polymorphism of blood protein pegagan duck by PAGE methodABSTRACT. Pegagan duck is one of local duck species which comes from Ogan Ilir (OI) Regency, South Sumatera. This species is not popular, yet. But, it has spread out along Ogan river which comprises of three districts e.g Tanjung Raja, Inderalaya and Pemulutan. The ecosystem in this three districts is dominated by swampy area. As far, there is relatively limited information of Pegagan duck compared to another local duck. So, for the first step was done a reseach to identify genetic characteristic from blood protein analyses by electrophoresis method. It is expected that this information will become the bases in determining of the policy of Pegagan duck development. Polymorphism analyses of blood protein was done with 9 duck samples which taken randomly from 400 pegagan ducks. Protein locus observed were albumin (Alb), post albumin (Pa), transferrine (Tf), post transferrine-1 (Ptf-1), and post transferrine-2 (Ptf-2). Polymorphism analyses of blood protein of pegagan duck was characterized by allele A in locus Transferrine (TfA), Post transferrine-1 (Ptf-1A) and Post transferrine-2 (Ptf-2A) . Genetic diversity based on blood protein of Pegagan duck showed that gen frequency value in a range of 0,11 0,94 and high heterozygosis value that was 0,62 %.
Identification of AHSG gene polymorphism and association with flavor and odor compounds of Indonesian lamb meat Septiyawan, Ganda Adi; Listyarini, Kasita; Noor, Ronny Rachman; Roosita, Katrin; Gunawan, Asep
Buletin Peternakan Vol 49, No 1 (2025): BULETIN PETERNAKAN VOL. 49 (1) FEBRUARY 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i1.89123

Abstract

Flavor and odor are important factors for consumers to consider the quality of lamb meat. The AHSG Alpha2-Heremans- Schmid Glycoprotein gene is associated with a fatty acid content and is indicated as a gene controlling flavor and odor compounds. The research aims to identify the AHSG gene polymorphism and its association with flavor and odor compounds in Indonesian lamb meat. The sample used in this study was 105 ram samples consisting of 10 Javanese Fat-Tailed Sheep (JFTS), 80 Javanese Thin-Tailed Sheep (JTTS), and 15 Jonggol sheep (JS). The AHSG gene polymorphism was validated using the PCR-RFLP technique with the EagI enzyme EagI. The association of the AHSG gene SNP (g.198655287 G>A) with odor and flavor was analyzed using the T-tests. The AHSG gene polymorphism in Indonesian lamb was polymorphic with three genotypes, i.e., GG, GA, and AA, and in the Hardy-Weinberg equilibrium. The AHSG significance was associated (P<0.05) with skatole compounds (MOA, EOA, MNA, and MI). The AA genotype is recommended for a candidate marker of flavors and odors because it associated with low compound values. The AHSG gene, specifically the SNP g.198655287 G>A, can potentially serve as a genetic marker for lamb meat production by reducing flavor and odor.
Stearoyl-CoA Desaturase (SCD) Gene Diversity and Its Association with Fatty Acids in Bali Cattle Using PCR-RFLP Method Sarwi, Annisa; Noor, Ronny Rachman; Jakaria, Jakaria
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 1 (2025): April 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.01.1

Abstract

This study examines the Stearoyl-CoA desaturase (SCD) gene, which is essential for converting saturated fatty acids (SFA) into monounsaturated fatty acids (MUFA). The research aims to analyze the diversity of Single Nucleotide Polymorphism (SNP) g.10428C>T within the SCD gene using the PCR-RFLP method. The investigation involved 40 blood samples from male Bali cattle aged 1.5 to 3 years, sourced from the Banjarmasin Slaughterhouse (RPH) in South Kalimantan. The SNP g.10428C>T diversity in the SCD gene was analyzed using the PCR-RFLP technique with the HinfI restriction enzyme. Genotype frequencies, allele frequencies, heterozygosity values, and the Hardy-Weinberg equilibrium were calculated using Popgen32. The relationship between fatty acid composition and the genotype of the SCD gene, as well as the fatty acids in Bali beef, was analyzed using a t-test. SCD|HinfI was found to have homozygous CC (0.03) and TT (0.85) and heterozygous CT (0.13) genotypes with a C allele frequency of 0.09 and T of 0.91. SNP g.10428C>T in the SCD gene was not significantly associated with fatty acid composition.
Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali Cattle Kholijah; Mokhmad Fakhrul Ulum; Sri Darwati; Ronny Rachman Noor; Jakaria
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 1 (2024): April 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.01.05

Abstract

This study aimed to assess the presence of single nucleotide polymorphisms (SNPs) in the exon 1 region of the tyrosinase (TYR) gene in Bali cattle with abnormal coat color. This study analyzed 43 Bali cattle, 26 individuals with typical or standard coat color, and 17 individuals with albinism. The genetic diversity of the TYR gene was determined via direct sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. The sequence data of the TYR gene were scrutinized using BioEdit and MEGA10 software to detect single nucleotide polymorphisms (SNPs) related to different types of coat color features in Bali cattle. The genetic diversity information was derived from computations performed using PopGen 1.32 software. The results showed that exon 1 of the TYR gene was affected by the new SNP g.939A>G, which is polymorphic in Bali cattle. In conclusion, the SNP c.939A>G can be further analyzed for use as a candidate-assisted selection (MAS) for abnormal coat color in Bali cattle.
Detection of the TYR Gene as a Candidate Causal Mutation for Albinism in Taro Cattle Reza, Muhammad Aulia; Noor, Ronny Rachman; Jakaria, Jakaria; Margawati, Endang Tri
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 2 (2024): August 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.02.12

Abstract

Bali Cattle are known as cattle with a unique coat color pattern. At Taro village was found different coat colors of Bali cattle, namely Taro cattle perform white coat color. The TYR gene has a vital role in producing coat color. This research was aimed to determine mutations that caused of the white color in Taro cattle. Two methods of PCR and PCR-RFLP were applied in this study. PCR method followed by sequencing used 29 DNA samples consisted of 17 Taro cattle, 12 DNA samples of 3 breeds cattle as comparison while RFLP method applied 56 DNA samples (17 Taro; 31 wild Bali; 4 Simental, 4 PO/Ongole descendant). All DNA were collected from fresh blood, and extracted by using DNA Extraction Kit. Amplification of TYR (exon 5) gene was conducted using forward and reverse primers to get polymerase chain reaction products with lengths of 320 bp and sequenced with Sanger method (services). PCR-RFLP was conducted with enzyme restriction of AciI. Determination of TYR gene diversity was analyzed through sequencing data. TYR gene sequencing was analyzed by using Bioedit and Mega11 software to identify single nucleotide polymorphisms (SNPs). SNP genotyping was analyzed from PCR-RFLP. The results showed that the TYR gene (exon 5) of Taro cattle was found with 320 bp length. SNP was obtained at one position c.1467 T>C. This SNP has never been reported yet on the ensemble website. This finding suggests that a specific genetic marker for albinism in Bali cattle needs to be more explored
Co-Authors . Subandriyo Adefia Fahira Arief Boediono Asep Gunawan ASEP SAEFUDDIN Cece Sumantri Chairun Nisa’ Chalid Talib DEWI APRI ASTUTI Dinar Tri Soelistyowati E. Damayanthi E. Damayanthi E. I. Rianti E. I. Rianti, E. I. Eko Handiwirawan Eko Handiwirawan ENDANG TRI MARGAWATI Epi Taufik Gisela Deviandini Heni Rizqiati Heni Rizqiati I Ketut Mudite Adnyana Irwan Irwan Jakaria Jakaria Katrin Roosita Kholijah Komar Sumantadinata Lia Budimulyati Salman Lies Emmawati Hadie Listyarini, Kasita Lucia Johana Lambey M Agus Setiadi Mala Nurilmala Meisji Liana Sari Mohamad Hasil Tamzil Mokhmad Fakhrul Ulum Odang Carman Peni S. Hardjosworo Ratna Sholatia Harahap Reza, Muhammad Aulia Rini Herlina Mulyono Rudy Priyanto Santiananda Arta Asmarasari Sarwi, Annisa Septiyawan, Ganda Adi Sri Darwati Srihadi Agungpriyono Subandriyo . . Subandriyo Subandriyo Subandriyo Subandriyo Suhendro, Ikhsan TAKDIR SAILI Talib, Chalid TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Wartono Hadie Wartono Hadie Wasmen Manalu Wenny Ladhunka Nur Aliyya