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All Journal Jurnal Ilmu Pertanian Indonesia Buletin Peternakan Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Animal Production : Indonesian Journal of Animal Production Informatika Pertanian Jurnal Penelitian Perikanan Indonesia Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Wahana Peternakan Jurnal Agripet Animal Production
Ronny Rachman Noor
Departemen Ilmu Produksi Dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor, Kampus IPB Darmaga Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Environmental Science Veterinary Other

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Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali Cattle Kholijah; Mokhmad Fakhrul Ulum; Sri Darwati; Ronny Rachman Noor; Jakaria
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 1 (2024): April 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.01.05

Abstract

This study aimed to assess the presence of single nucleotide polymorphisms (SNPs) in the exon 1 region of the tyrosinase (TYR) gene in Bali cattle with abnormal coat color. This study analyzed 43 Bali cattle, 26 individuals with typical or standard coat color, and 17 individuals with albinism. The genetic diversity of the TYR gene was determined via direct sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. The sequence data of the TYR gene were scrutinized using BioEdit and MEGA10 software to detect single nucleotide polymorphisms (SNPs) related to different types of coat color features in Bali cattle. The genetic diversity information was derived from computations performed using PopGen 1.32 software. The results showed that exon 1 of the TYR gene was affected by the new SNP g.939A>G, which is polymorphic in Bali cattle. In conclusion, the SNP c.939A>G can be further analyzed for use as a candidate-assisted selection (MAS) for abnormal coat color in Bali cattle.
Detection of the TYR Gene as a Candidate Causal Mutation for Albinism in Taro Cattle Reza, Muhammad Aulia; Noor, Ronny Rachman; Jakaria, Jakaria; Margawati, Endang Tri
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 2 (2024): August 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.02.12

Abstract

Bali Cattle are known as cattle with a unique coat color pattern. At Taro village was found different coat colors of Bali cattle, namely Taro cattle perform white coat color. The TYR gene has a vital role in producing coat color. This research was aimed to determine mutations that caused of the white color in Taro cattle. Two methods of PCR and PCR-RFLP were applied in this study. PCR method followed by sequencing used 29 DNA samples consisted of 17 Taro cattle, 12 DNA samples of 3 breeds cattle as comparison while RFLP method applied 56 DNA samples (17 Taro; 31 wild Bali; 4 Simental, 4 PO/Ongole descendant). All DNA were collected from fresh blood, and extracted by using DNA Extraction Kit. Amplification of TYR (exon 5) gene was conducted using forward and reverse primers to get polymerase chain reaction products with lengths of 320 bp and sequenced with Sanger method (services). PCR-RFLP was conducted with enzyme restriction of AciI. Determination of TYR gene diversity was analyzed through sequencing data. TYR gene sequencing was analyzed by using Bioedit and Mega11 software to identify single nucleotide polymorphisms (SNPs). SNP genotyping was analyzed from PCR-RFLP. The results showed that the TYR gene (exon 5) of Taro cattle was found with 320 bp length. SNP was obtained at one position c.1467 T>C. This SNP has never been reported yet on the ensemble website. This finding suggests that a specific genetic marker for albinism in Bali cattle needs to be more explored
Co-Authors . Subandriyo Adefia Fahira Arief Boediono Asep Gunawan ASEP SAEFUDDIN Cece Sumantri Chairun Nisa’ Chalid Talib DEWI APRI ASTUTI Dinar Tri Soelistyowati E. Damayanthi E. Damayanthi E. I. Rianti E. I. Rianti, E. I. Eko Handiwirawan Eko Handiwirawan ENDANG TRI MARGAWATI Epi Taufik Gisela Deviandini Heni Rizqiati Heni Rizqiati I Ketut Mudite Adnyana Irwan Irwan Jakaria Jakaria Katrin Roosita Kholijah Komar Sumantadinata Lia Budimulyati Salman Lies Emmawati Hadie Listyarini, Kasita Lucia Johana Lambey M Agus Setiadi Mala Nurilmala Meisji Liana Sari Mohamad Hasil Tamzil Mokhmad Fakhrul Ulum Odang Carman Peni S. Hardjosworo Ratna Sholatia Harahap Reza, Muhammad Aulia Rini Herlina Mulyono Rudy Priyanto Santiananda Arta Asmarasari Sarwi, Annisa Septiyawan, Ganda Adi Sri Darwati Srihadi Agungpriyono Subandriyo . . Subandriyo Subandriyo Subandriyo Subandriyo Suhendro, Ikhsan TAKDIR SAILI Talib, Chalid TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Wartono Hadie Wartono Hadie Wasmen Manalu Wenny Ladhunka Nur Aliyya