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Ekstrak Etanol Kulit Buah Naga Menurunkan Indikasi Neoplasia Mammae Tikus Putih Berdasarkan Histopatologi dan Inhibitor Siklooksigenase-2 Muhammad Thohawi Elziyad Purnama; Ragil Angga Prastiya; Faisal Fikri; Amung Logam Saputro; Bodhi Agustono
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.173 KB) | DOI: 10.19087/jveteriner.2018.19.1.23

Abstract

Cancer caused uncontrolled cell proliferation and triggered by changes on cell information that regulate growth function. Each cell has different potential so many cancer prevalence such as endometrial cancer, lymph node cancer, lung cancer, skin and mammary gland cancer. The aims of this study were to assess the potential of dragon fruit skin ethanolic extract on white rats (Rattus norvegicus) exposed 7,12- Dimethylbenz(á)antrasena (DMBA) on mammary gland based on histopathological features and cyclooxygenase-2 (Cox-2) intensity. This study were used 20 of rats randomly divided into five group and each groups consisted of four rats, i.e: K+ weren’t treated DMBA and extract; K- were treated with DMBA; P1 were treated with DMBA and extract 10 mg/kg BW; P2 were treated with DMBA and extract 15 mg/kg BW; P3 were treated with DMBA and extract 20 mg/kg BW. The DMBA was given by intra dermal injection during twice a week for five weeks and the extracts with gastric tube everyday till 14 days. The data was analyzed by Anova test and continued with Duncan test. The result showed that the histopathological features were decrease significantly on P3. The variables of Cox-2 intensity were decrease significantly on P1, P2 and P3. Conclusion of this study was ethanol extract of dragon fruit skin can decrease neoplastic indication of mammary gland on white rats (R. norvegicus) based on histopathological features and Cox-2 inhibitors.
Effect of Cirripedia sp. Flour As Subtitutional Feed Source of Protein Against Dried Disease and Organic Material Rabbit Rain Type Rex Bodhi Agustono; Widya Paramita Lokapirnasari; Mohammad Anam Al Arif; Ragil Angga Prastiya; Faisal Fikri
Bantara Journal of Animal Science Vol 2, No 1 (2020)
Publisher : Program Studi Peternakan, Fakultas Pertanian, Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/bjas.v2i1.636

Abstract

The aim of this research was to determine the effect of Cirripedia sp. flour as feed substitution of protein source in feed on the digestibility of dry matter and organic matter in 100 male rex broilers. The research was experimental by using a completely randomized design with four treatments and five replications. Substitution of Cirripedia sp. flour respectively in feed were 0%, 2.5%, 5%, 7.5% given for four weeks. Data collection was carried out in the fourth week (for seven days) which included consumption data, manure weight and analysis of treatment feed and manure analysis. The data were analyzed using Analysis of Variance and the difference between treatments was determined by the Duncan test. The results showed that there was no significant difference (p>0.05) and that there was no significant difference (p>0.05) on the digestibility of rex broilers. This shows that the substitution of Cirripedia sp. flour in feed can replace animal protein sources in fish meal in complete feed rabbits.Key words : Cirripedia sp, digestibility, feed subtitution, flour, rabbit.
Molecular Identification of Pathogenic Leptospirosis with Zoonotic Potential in Urban Rats (Rattus Rattus) in Banyuwangi District Maya Nurwartanti Yunita; Bodhi Agustono; Muhammad Thohawi Elziyad Purnama
Bantara Journal of Animal Science Vol 2, No 1 (2020)
Publisher : Program Studi Peternakan, Fakultas Pertanian, Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/bjas.v2i1.639

Abstract

Leptospirosis is a zoonotic disease that occurs globally. Leptospires are transmitted through the urine of infected mice, through injured skin or mucosal invasion. Infection can occur through direct contact or through contact with contaminated water or soil. This qualitative research uses a combination of epidemiological studies with a case study approach. Rat urine metabolism samples will be tested by testing the base pair chain through the Polymerase Chain Reaction method for leptospirosis. Electrophoresis results showed 5% percent of positive samples with 330 bp. The conclusion of this study, the molecular identification of leptospirosis from rat urine using the Polymerase Chain Reaction technique are found positive results so prevention is needed to control the distribution of pathogenic leptospirosis bacteria. Urban rats (Rattus rattus) in leptospirosis transmission are considered as the most important leptospira reservoir, because high population densities of this species can cause an increase in the spread of leptospirosis. Keywords: Banyuwangi, Leptospirosis, Urban rats, Zoonosis
Gambaran Histologi Lebar Vili Jejunum Kelinci Jenis Rex dengan Subtitusi Bahan Penyusun Tepung Ikan dengan Tepung Teritip (Cirripedia sp.) Maya Nurwartanti Yunita; Bodhi Agustono; Amung Logam Saputro
Jurnal Nutrisi Ternak Tropis Vol 3, No 1 (2020): JNT | Jurnal Nutrisi Ternak Tropis Maret 2020
Publisher : Jurnal Nutrisi Ternak Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2020.003.01.6

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tepung teritip (Cirripedia sp.) Sebagai pakan pengganti dari tepung ikan pada pakan kelinci, seperti yang diamati dari lebar jejunum villi. Kelinci sebanyak 100 ekor ditempatkan pada kandang individu dengan pakan standart yang sesuai kebutuhan mereka secara ad libitum. Persentase feed substitusi dengan tepung teritip dengan empat perlakuan dan lima replikasi dalam pakan sebesar 0%, 2,5%, 5%, 7,5% pada masing-masing kelompok. Perlakuan dilakukan selama 4 minggu, data dikumpulkan setelah pengorbanan akhir perlakuan. Data akhir penelitian ini dianalisis menggunakan analisis variance dengan angka tingkat signifikansi 0,05. Hasil analisis data menunjukkan bahwa tidak ada signifikansi (p> 0,05) pada lebar vili jejunum. Penggunaan tepung teritip sebagai pengganti pakan untuk tepung ikan pada kelinci. Pakan lengkap berpengaruh signifikan terhadap lebar vili jejunum.
Pengaruh Subtitusi Tepung Ikan Dengan Tepung Bernacle Terhadap Berat Organ Pencernaan dan Lemak Abdominal Kelinci Pedaging Jantan (Orytolagus cuniculus) Bodhi Agustono; Maya Nurwartanti Yunita; Sunaryo Hadi Warsito; Widya Paramita Lokapirnasari; Mohammad Anam Al Arif; Ragil Angga Prastiya; Amung Logam Saputro
Jurnal Nutrisi Ternak Tropis Vol 3, No 1 (2020): JNT | Jurnal Nutrisi Ternak Tropis Maret 2020
Publisher : Jurnal Nutrisi Ternak Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2020.003.01.8

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tepung teritip (Cirripedia sp.) Sebagai pengganti pakan dari tepung ikan pada pakan kelinci, seperti yang diamati dari lemak abdominal dan berat organ pencernaan kelinci jantan rex. Kelinci sebanyak 100 ekor ditempatkan pada kandang individu dengan pakan standart yang sesuai kebutuhan mereka secara ad libitum. Persentase feed substitusi dengan tepung teritip dengan empat perlakuan dan lima replikasi dalam pakan P0 (TI 15% dan TT 0%), P1 (TI 12,5% dan TT 2,5%), P2 (TI 10% dan TT 5%), P3 (TI 7,5% dan TT 7,5%). Perlakuan dilakukan selama 4 minggu, data dikumpulkan setelah pengorbanan akhir perlakuan. Usus kecil kelinci dikumpulkan dan menimbang organ pencernaan termasuk lambung, usus kecil dan usus besar. Hasil penelitian dianalisis menggunakan analisis varians dengan tingkat signifikansi 0,05. Berdasarkan hasil analisis menunjukkan bahwa tidak ada perbedaan yang signifikan (p> 0,05) pada lemak perut. Hasil penelitian menunjukkan tidak ada perbedaan yang signifikan (p> 0,05) dalam berat organ pencernaan (lambung, usus kecil, dan usus besar). Tepung teritip dapat digunakan sebagai pakan pengganti tepung ikan dalam pakan lengkap untuk kelinci jenis rex hingga 7,5%.
The Effect of Buriti Oil (Mauritia flexuosa L.) as Feed Additive on the Organic and Inorganic Materials Digestibility on Heat Stress Exposed Broilers Burhanudin; Sunaryo Hadi Warsito; Bodhi Agustono; Nusdianto Triakoso; Ragil Angga Prastiya; Faisal Fikri
Jurnal Agro Veteriner Vol. 7 No. 2 (2024): Journal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i2.57618

Abstract

Heat stress due to high environmental conditions can affect the digestibility of organic and inorganic broilers. Buriti oil (Mauritia flexuosa L.) contains antioxidants that can suppress Reactive Oxygen Species (ROS) thereby reducing the negative effects of heat stress. This study aims to determine the effect of adding buriti oil to the ration on the organic and inorganic matter of digestibility in broiler exposed to heat stress. This research was experimental and the method used is the completely randomized design method (CRD). The treatment be carried out on 20 broiler aged 22-35 days who are kept in individual cages. Broiler in the treatment group were given heat stress induction using gasolec up to a temperature 35 ± 1o°C for 4 hours every day. Broiler were randomly selected in 4 treatment groups divided into K- (temperature 27 ± 2°C + complete feed), K+ (exposure to temperature 35 ± 1°C + complete feed), P1 (exposure to temperature 35 ± 1°C + complete feed + 2% buriti oil total feed gram /head /day) and P2 (exposure to temperature 35 ± 1°C + complete feed + 4% buriti oil total feed gram /head /day).
First report of Ophidascaris spp. (Class: Nematode) Infection in Wild-Caught Javanese Keelback Water Snake (Fowlea melanzostus) in Banyuwangi District Yudhana, Aditya; Salsabila Putri, Elma; Angga Prastiya, Ragil; Nurwartanti Yunita, Maya; Agustono, Bodhi; Ayu Wibawati, Prima
Journal of Parasite Science Vol. 8 No. 1 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i1.54578

Abstract

The javanese keelback water snake (Fowlea melanzostus) is a semi-aquatic reptile that often found in Indonesia and endemic to Java island. Water tiger snakes are usually kept as exotic pets. Wild caught javanese keelback water snakes have risks of spreading several disease agents that can be zoonotic, which is nematodiasis caused by Ophidascaris spp. This study aims to determine the prevalence level of Ophidascaris spp. infections in javanese keelback water snakes from Banyuwangi district. This study used a descriptive method with accidental sampling. The total sample in this study was 33 wild-caught javanese keelback water snakes. The identification method used is snake samples that have been collected are then euthanized and necropsied to check for nematode infections in the subcutan, muscular, and visceral part. The result showed that 28 samples were positive for nematode infection and 5 samples were negative with a prevalence rate of 84.84%. The nematodes that have been identified come from the genus Ophidascaris spp. Therefore, further research is needed to determine the incidence of nematode transmission in other wild caught snakes in Indonesia.
Identifikasi Limbah Pertanian dan Perkebunan Sebagai Bahan Pakan Inkonvensional Di Banyuwangi Agustono, Bodhi; Lamid, Mirni; Ma'ruf, Anwar
Jurnal Medik Veteriner Vol. 1 No. 1 (2017): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.369 KB) | DOI: 10.20473/jmv.vol1.iss1.2017.12-22

Abstract

Penelitian ini bertujuan untuk mengumpulkan data bahan pakan inkonvensional dari limbah pertanian dan perkebunan di Kabupaten Banyuwangi. Pakan merupakan aspek terpenting dalam pengelolaan peternakan. Ketersediaan pakan baik di musim hujan maupun musim kemarau harus tetap ada. Penelitian ini menggunakan metode purposive sampling sehingga sampel diambil di Kecamatan Kalibaru, Genteng, Purwoharjo, Songgon, Banyuwangi, Licin, Glagah dan Wongsorejo. Sampel bahan selanjutnya dianalisis proksimat untuk mengetahui kadar serat kasar (SK), protein kasar (PK), lemak kasar (LK), bahan ekstrak tanpa nitrogen (BETN) dan kadar abu. Hasil menunjukkan bahwa limbah jerami padi, jerami kedelai, nanas, rumput gajah, alang-alang, rumput lapang, kaliandra, turi dan lamtoro memiliki dominasi kandungan gizi yang bervariasi. Simpulan penelitian bahwa bahan limbah dapat diformulasikan sebagai pakan ternak inkonvensional.
Motilitas dan Viabilitas Spermatozoa Sapi Rambon di Desa Kemiren Banyuwangi Prastika, Zeni; Susilowati, Suherni; Agustono, Bodhi; Safitri, Erma; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 1 No. 2 (2018): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.103 KB) | DOI: 10.20473/jmv.vol1.iss2.2018.38-42

Abstract

Sapi Rambon merupakan salah satu sapi lokal Indonesia. Tahun 2017 populasi sapi Rambon mengalami penurunan. Salah satu upaya pelestarian sapi lokal adalah melalui peningkatan populasi sapi dan inseminasi buatan. Faktor penentu keberhasilan perkawinan salah satunya adalah libido tinggi dan kualitas semen. Motilitas spermatozoa dan viabilitas spermatozoa adalah salah satu indikator fertilitas spermatozoa dan kualitas semen. Penelitain ini adalah penelitian deskriptif. Penelitian ini bertujuan untuk mengetahui motilitas dan viabilitas semen segar sapi Rambon di Desa Kemiren Kecamatan Glagah Kabupaten Banyuwangi. Semen pada penelitian ini di tampung menggunakan vagina buatan dari 6 ekor sapi Rambon jantan. Pemeriksaan motilitas spermatozoa dan viabilitas spermatozoa dilakukan di klinik Linawati Banyuwangi. Hasil pada penelitian ini disajikan dalam bentuk tabel. Parameter penelitian ini adalah motilitas dan viabilitas spermatozoa sapi Rambon. Hasil pemeriksaan motilitas spermatozoa sapi Rambon 83,3±4,1% dan viabilitas spermatozoa sapi Rambon 86,7±2,3%. Kesimpulan dari penelitian menunjukan bahwa motilitas dan viabilitas spermatozoa sapi Rambon baik dan memenuhi syarat semen beku untuk inseminasi buatan.
Pengaruh Substitusi Tepung Buah Hala (Pandanus tectorius) dalam Pelet Terhadap Konsumsi Bahan Kering dan Bobot Kelinci Pedaging Saraswati, Adyayutti Wijang; Al Arif, Mohammad Anam; Wibawati, Prima Ayu; Damayanti, Ratna; Agustono, Bodhi
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.515 KB) | DOI: 10.20473/jmv.vol1.iss3.2018.57-61

Abstract

Tujuan pada penelitian ini adalah untuk mengetahui efek substitusi tepung buah hala dalam pelet pada konsumsi bahan kering dan pertambahan bobot badan harian pada kelinci jantan ras Flemish giant. Metode penelitian eksperimental ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 pengulangan. Penelitian ini menggunakan 20 ekor kelinci ras Flemish giant jantan berumur 1,5-2 bulan dengan bobot rata-rata 750 gram. Kelompok perlakuan pakan adalah P0 (100% pakan komersil), P1 (85% pakan komersil dengan 15% tepung buah hala), P2 (75% pakan komersil dengan 25% tepung buah hala), P3 (60% pakan komersil dengan 40% tepung buah hala) dan diberikan sebanyak 150 gram/hari untuk setiap kelompok. Data yang didapat dianalisa menggunakan ANOVA dan dilanjutkan dengan uji Duncan multiple test. Hasil menunjukkan perbedaan yang signifikan (p<0,05) pada konsumsi bahan kering, namun tidak pada pertambahan bobot badan harian kelinci (p>0,05). Rata-rata konsumsi bahan kering/hari pada kelompok P0 hingga P3 adalah 121,088±0,57 gram; 120,12±2,11 gram; 119,98±0,87 gram; 118,19±0,67 gram. Rata-rata pertambahan bobot badan harian pada P0 hingga P3 adalah 84±0,79 gram; 83,1±0,89 gram; 78,6±2,84 gram; 74,5±2,09 gram. Pada penelitian ini menunjukkan bahwa kandungan tepung buah hala sebanyak 25% pada pakan tidak terdapat perbedaan pada konsumsi bahan kering dan pertambahan bobot kelinci bila dibandingkan dengan pelet komersil.