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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Veteriner Jurnal Biologi Udayana Biotika: Jurnal Ilmiah Biologi Biotropika JURNAL ISTEK Pro-Life Kumawula: Jurnal Pengabdian Kepada Masyarakat KAIBON ABHINAYA : JURNAL PENGABDIAN MASYARAKAT Indonesian Journal of Limnology Farmers: Journal of Community Services
Nining Ratningsih
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Social Sciences Veterinary Other

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Identifikasi Ikan Hasil Tangkapan Nelayan di Pantai Timur Pananjung Pangandaran Kartiawati Alipin; Nining Ratningsih; Retnaningtyas Siska Dianty
Biotropika: Journal of Tropical Biology Vol 9, No 2 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2021.009.02.05

Abstract

Kawasan Pantai Pangandaran merupakan kawasan wisata dan penangkapan ikan bagi nelayan. Hasil tangkapan nelayan terdiri dari berbagai spesies ikan. Untuk mengetahui hasil tangkapan nelayan tersebut perlu dilakukan identifikasi spesies ikan. Tujuan penelitian ini adalah untuk mengidentifikasi spesies ikan berdasarkan struktur morfologis dan morfometrik. Metode penelitian yang dilakukan yaitu pengamatan secara morfologis dan morfometrik menggunakan sampel ikan masing-masing tiga ekor jenis ikan yang mewakili ikan terbanyak pada hasil tangkapan nelayan. Berdasarkan hasil identifikasi jenis ikan yang paling banyak dari hasil tangkapan nelayan didapat spesies ikan laut Kembung (Rastrelliger brachysoma) yang memiliki ekor berwarna kuning; ikan Talang (Scomberoides tol) yang memiliki corak keperakkan di sepanjang tubuh; dan ikan Kakapasan (Geres punctatus) yang memiliki bentuk mulut superior yang khas. Berdasarkan pengukuran morfometrik terdapat spesies ikan yang memiliki ukuran paling besar yaitu ikan laut Kembung (R. brachysoma) dengan panjang total 25,1 cm dan berat rata-rata 215 g. Kesimpulan penelitian menunjukkan bahwa hasil tangkapan nelayan di Pantai Timur Pananjung Pangandaran berdasarkan hasil identifikasi terdapat tiga jenis ikan yang paling dominan untuk itu perlu dilakukan pemeliharaan kawasan agar memungkinkan berbagai jenis ikan dapat berkembang biak.
UJI SENSITIVITAS BAKTERI STAPHYLOCOCCUS AUREUS, STREPTOCOCCUS PYOGENES DAN PSEUDOMONAS AERUGINOSA TERHADAP AIR REBUSAN CACING TANAH LUMBRICUS RUBELLUS DAN PHERETIMA ASIATICA DAN ANTIBIOTIK SECARA IN VITRO Ida Indrawati; Nining Ratningsih; Sadiah Djajasupena
JURNAL ISTEK Vol 7, No 2 (2013): ISTEK
Publisher : JURNAL ISTEK

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Telah dilakukan penelitian mengenai uji sensititvitas bakteri S.aureus, S. pyogenes dan P.aeruginosa terhadap antibiotik uji dan air rebusan cacing tanah secara in vitro. Hasil penelititan dengan diameter daerah hambat sebagai parameter dan dilakukan secara eksperimental dengan rancangan acak lengkap pola faktorial ini menunjukan bahwa bakteri S.aureus lebih sensitif terhadap kloramfenikol dan gentamicin, S.pyogenes lebih sensititif terhadap basitracin dan P. aeruginosa resisten terhadap keempat antibiotik uji. Selanjutnya, bakteri S. aureus lebih sensitif terhadap air rebusan cacing tanah P.asiatica daripada air rebusan L.rubellus. Sementara S. pyogenes dan P. aeruginosa lebih sensititf terhadap air rebusan L.rubellus daripada air rebusan P.asiatica. Konsentrasi air rebusan cacing tanah yang memberikan hambatan yang efektif terhadap S.aureus adalah konsentrasi 80 %, S.pyogenes dimulai dari konsentrasi 20 % dan P.aeruginosa dimulai dari konsentrasi 2,5 %. Bakteri S.aureus dan S.pyogenes lebih sensitif terhadap antibiotik sementara P. aeruginosa lebih sensitif terhadap air rebusan cacing tanah.
Perilaku Kawin Tikus (Rattus norvegicus) Jantan Diabetes Yang Diberi Ekstrak Etanol Kulit Buah Jengkol (Archidendron pauciflorum) Desak Made Malini; Emay Maulani; Sri Wulandari; Nining Ratningsih
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p17

Abstract

Diabetes mellitus (DM) may lead to disruption of normal sexual function in males including abnormalities of orgasmic/ejaculatory function and desire/libido. Simplisia of dried Jengkol fruit peel has been used by people to treat diabetes. The aim of this study was to examine the effectiveness of ethanol extract of jengkol fruit peel (A. pauciflorum) (EEJFP) in improving the sexual behavior of male rats (R. norvegicus) diabetic models. Induction of diabetes was carried out intravenously by using streptozotocin dose 60 mg/ kg BW in the test animals except for the negative control group.The treatments are negative control (Carboxy Methyl Cellulose (CMC) 0.05%), positive control (CMC 0.05%), P1 (EEJFP 385 mg/kg BW), P2 (EEJFP 770 mg/kg BW) and comparison (glibenclamide dose 10 mg/kg BW) for 54 days. The parameters observed are the number of introduction and climbing of male rats to female rats. Data obtained from each parameter were analyzed by analysis of variance (ANAVA) test with a 95% confidence level and Duncan's test. The results of this study indicate that the number of introductions and climbing in the P1 and P2 test animal groups are significantly different from the positive control treatment and close to the value in the negative control treatment group. It can be concluded that the ethanol extract of jengkol fruit peel (A. pauciflorum) affects the sexual behavior of male rats (R. norvegicus) diabetes models by increasing introduction and climbing. Keyword: diabetic, sexual behavior, Archidendron Pauciflorum, rats
Ekstrak Etanol Kulit Buah Jengkol Menurunkan Kadar Glukosa dan Meningkatkan Hormon Insulin Tikus Diabetes Yang Diinduksi Streptozotocin Desak Made Malini; Madihah Madihah; Dita Aprila Khoirunnisa; Indriani Sasmita; Nining Ratningsih; Kartiawati Alipin; Wawan Hermawan
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.755 KB) | DOI: 10.19087/jveteriner.2019.20.1.65

Abstract

Diabetes Mellitus (DM) is a disease with metabolism disorder signed by increasing of glucose blood (hyperglicemia), caused by disorder of insulin secretion and or increasing of insulin resistance. Therapy of jengkol’s fruit peel is an alternative treatment for DM. This study aims to determine the effect of ethanol extract of jengkol’s fruit peel to decrease glucose and increase insulin level of Wistar female rats which induced by streptozotocin (STZ). This research used the experimental method of Completely Randomized Design (RAL). Rat were first induced by single intravenous injection of STZ except Negative Control. Furthermore, rats were divided into six groups, namely Negative Control (NC), Positive Control (PC), Reference Group (RG) glibenclamide 10 mg/kg BW, and ethanol extract of jengkol’s fruit peel (EEOJFP) treatment at dose of 385 (P1), 770 (P2), and 1540 (P3) mg/kg BW with four replications each. Treatment was administered orally for 14 days. Data were analyzed by analysis of variance (ANAVA) with 95% confidence level and Duncan Multiple Range Test. The result showed that treatment of EEOJFP with dose of 385, 770 and 1540 mg/ kgBW decreased blood glucose 28.6%;65.4% and 59.1% successively, increased insulin as 54%; 230% and 206%. There was a protective effect of EEOJEF to level of blood glucose and insulin secretion of rat-STZ induced. The conclusion from this research is Ethanol extract of Jengkol’s fruit peel at dose 770 mg/kg BW is an effective dose in decreasing the level of glucose and increasing the level of insulin hormone in STZinduced diabetic rats.
Struktur morfologis dan histologis ginjal tikus model diabet setelah diberi ekstrak etanol kulit buah jengkol (Archidendron pauciflorum) Desak Made Malini; Nurullia Fitriani; Adnin Laila; Nining Ratningsih; Tia Setiawati
Jurnal Biologi Udayana Vol 25 No 2 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i02.p13

Abstract

In a long term, diabetes mellitus (DM) leads to nephropathy due to glomerular hyperfiltration. One of the plant used as a diabetic drug by the community in Karangwangi Village, Cianjur Regency, West Java is the fruit peel of jengkol. Therefore, this study aims to determine the effect of the ethanolic extract of Jengkol fruit peel (EEJFP) toward the morphological and histological structure on the kidney of the diabetic rat model. The method adopted was the Randomized Design (CRD) with 6 treatments namely NC (Carboxyl Methyl Cellulose (CMC) 0.5%), PC (CMC 0.5%), Pb (Glibenclamide 5 mg/kg BW), P1, P2, and P3 (EEJFP 385; 770; and 1,540 mg/kg BW) with 4 replications for 14 consecutive days. Furthermore, the induction of diabetes with streptozotocin dose of 60 mg/Kg BW was performed intravenously in experimental animals except for the NC group. The parameters observed include relative weight, morphological, and histological structure of kidney which include glomerular diameter, Bowman space distance, and percentage of proximal tubular cell necrosis. The non-parametric and parametric data were tested by Kruskal Wallis and ANOVA test as well as Duncan's follow-up test, respectively. The results showed that there was no significant difference in the morphological structure of the kidney between treatment groups. Furthermore, the relative weights of kidney in the PC, Pb, P1, and P3 groups were larger and significantly different compared to NC and P2 also, the histological structure showed that the glomerular diameter (65.43 ± 0.7 m), Bowman space distance (4.19 ± 1.7 µm), and the percentage of proximal tubular cell necrosis (24.6 ± 5.5%) at P2 were not significantly different from NC. Based on this results, it was concluded that EEJFP has no effect on the kidney’s morphological structure, however, it decreases its relative weight and repair the kidney’s histological damage of the diabetic rat model with the optimum dose of 770 mg/kg BW.
POTENSI REGENERASI SEL SERTOLI DAN SEL LEYDIG TIKUS (Rattus norvegicus) MODEL DIABETES PASCA PEMBERIAN EKSTRAK ETANOL KULIT BUAH JENGKOL (Archidendron pauciflorum) Desak Made Malini; Nining Ratningsih; Nurullia Fitriani; Dwi Rahmi
Jurnal Pro-Life Vol. 7 No. 2 (2020): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Diabetes mellitus (DM) is a metabolic disease that causes disruption of spermatogenesis due to decreased numbers of Sertoli cells and Leydig cells. The aim of this study was to determine the potency of ethanol extract of Jengkol fruit peel (JFPEE) on increasing the regeneration of Leydig cells and Sertoli cells in diabetic rat models. This type of research is experimental research using a completely randomized design (CRD)with 5 treatments and 5 replications. Treatment was given orally for 54 consecutive days consisting of negative control (NC), positive control (PC), comparison (Glibenclamide dose 10 mg/kg BW), P1 and P2 (JFPEE dose 385 and 770 mg/kg BW). Diabetic induction was performed with streptozotocin dose 65 mg/kg BW in male Wistar rat except for NC group. The parameters observed were the number of Sertoli cells and Leydig cells in 25 seminiferous tubules.The results of histological structured showed that the highest number of Sertoly cells and Leydig cells were obtained in group P2 (4.40±0.55; 9.80±0.84) and it was not significantly different from the NC group (5.00±1.41; 12.20±2.77). It can be concluded that 770 mg/kg BW was the effective dose of JFPEE that can increase the regeneration of Sertoli cells and Leydig cells in diabetic rat models.Keywords: Jengkol Fruit Peel Ethanol Extract, Leydig Cells, Regeneration, Sertoli Cells.
ANALISIS PERTUMBUHAN DAN KANDUNGAN TOTAL FLAVONOID KULTUR KALUS KRISAN (Chrysanthemum morifolium Ramat) DENGAN PEMBERIAN ASAM 2,4-DIKLOROFENOKSIASETAT (2,4-D) DAN AIR KELAPA Tia Setiawati; Anggita Levi Astuti; Mohamad Nurzaman; Nining Ratningsih
Jurnal Pro-Life Vol. 8 No. 1 (2021): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Chrysanthemum morifolium contains flavonoids which have antioxidant properties and was useful in treating various diseases. The production of flavonoids from plants can be done in vitro through callus culture. Auxin 2,4-D growth regulators and organic compounds such as coconut water added to the media can affect the success of callus formation and secondary metabolite synthesis. The purpose of this study was to obtain the best treatment of 2,4-D concentration and coconut water that can increase the growth and total flavonoid levels of C. morifolium callus cultures. The method used in this research was the experimental method completely randomized design (CRD) with 8 treatments, namely 1 ppm 2,4-D + 10% coconut water (CW); 2 ppm 2,4-D + 10% CW; 3 ppm 2,4-D + 10% CW ; 4 ppm 2,4-D + 10% CW; 1 ppm 2,4-D; 2 ppm 2,4-D; 3 ppm 2,4-D; 4 ppm 2,4-D in 4 replication. The results showed that the treatment of 4 ppm 2,4-D and 10% CW was the best treatment combination, resulting in an average callus size (1.4 cm), fresh weight (0.19 grams), dry weight (0.16 gram) and total flavonoid content (1.873 mgQE/g)
Pemberian Asam Humat untuk Meningkatkan Pertumbuhan dan Kandungan Total Flavonoid Sawi Hijau (Brassica juncea L.) pada Perbedaan Kadar Salinitas Tia Setiawati; Tentani Buhti Amadea; Mohamad Nurzaman; Nining Ratningsih
Jurnal Pro-Life Vol. 8 No. 3 (2021): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Salt affects plant growth due to increased soil osmotic pressure and plant nutrients disturbance. Most plants are susceptible to high salinity which can cause physiological and biochemical interference. This research was conducted to obtain a possible salinity level for mustard greens (Brassica juncea L.) to grow well and the best humic acid dosage to reduce salinity damages and to gain high total flavonoid content. This research used Randomized Block Design method with two factors and four replications. The factors are salinity levels (control, 50, 75, and 100 mM) and humic acid dosage (control, 2, 4, and 8 g/kg). In the parameters which had interactions between the two treatments, the highest results were obtained with a combination of 100 mM salinity and 12 g humic acid, such as plant height (33.35 cm), leaf area (379.66 cm2), fresh weight (22.41 g) and dry weight (1.54 g). The highest results on leaf number (8.5 leaves) and total flavonoid content (0.074 mgQE/g sample) were obtained with 100 mM salinity. Humic acid dose of 12 g gave the highest result on leaf number (8.69 leaves) and total flavonoid content (0.095 mgQE/g sample).
Empowering the Rural Communities through Education on the Use of Chrysanthemums as Medicinal Plants and Herbal Tea Tia Setiawati; Annisa Annisa; Mohamad Nurzaman; Rusdi Hasan; Nining Ratningsih
KAIBON ABHINAYA : JURNAL PENGABDIAN MASYARAKAT Vol. 5 No. 1 (2023)
Publisher : Universitas Serang Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30656/ka.v5i1.5347

Abstract

Chrysanthemum is widely known as ornamental plants, but chrysanthemums can also be used as medicinal plants and can be processed into healthy beverages known as chrysanthemum tea. The purpose of the PPM activity is to educate the community, especially PKK women who are participants in this activity about the role of chrysanthemum plants in the prevention and treatment of various diseases such as fever, hypertension, osteoporosis, eye healthy and making chrysanthemum tea as a healthy beverage. PPM activities was carried out through outreach activities with presentations and discussions (question and answer), demonstrations of making chrysanthemum tea, and distributing questionnaires to determine participants understanding before and after the implementation of PPM activities. The results of the questionnaire showed that participants knowledge about the use of chrysanthemum as medicinal ingredients increased after participating in outreach activities, as well as the interest in chrysanthemum tea cultivation and entrepreneurship.
Efek Kombinasi Ekstrak Temulawak dan Belimbing Wuluh terhadap Perbaikan Histologis Ginjal Tikus yang Terpapar Herbisida Paraquat Kartiawati Alipin; Nining Ratningsih; Salsabila Nurjihan
BIOTIKA Jurnal Ilmiah Biologi Vol 20, No 2 (2022): BIOTIKA DESEMBER 2022
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v20i2.41713

Abstract

Paraquat merupakan herbisida yang digunakan pada pertanian. Akumulasi paraquat pada tanaman sayur jika dikonsumsi dapat bersifat racun dan menyebabkan gangguan pada organ ginjal seperti kerusakan pada glomerulus berupa edema spatium Bowman, serta pada tubulus berupa infiltrasi sel radang dan pembengkakan sel epitel tubulus. Tujuan penelitian ini yaitu untuk mengetahui pengaruh pemberian kombinasi ekstrak temulawak dan belimbing wuluh (TBW) terhadap perbaikan histologis ginjal tikus yang terpapar paraquat. Metode penelitian secara eksperimental menggunakan Rancangan Acak Lengkap dengan lima perlakuan dan lima ulangan. Induksi paraquat dilakukan pada seluruh perlakuan kecuali KN. Susunan perlakuan yang terdiri dari KN (tween 80 1%), KP (paraquat 25 mg/KgBB), P1 (kombinasi TBW 770 mg/KgBB), P2 (kombinasi TBW 385 mg/KgBB), P3 (kombinasi TBW 192,5 mg/KgBB). Parameter yang diamati yaitu histologis ginjal meliputi diameter glomerulus, lebar ruang Bowman, dan persentase nekrosis pada tubulus proksimal. Data dianalisis secara statistik menggunakan uji ANOVA pada taraf kepercayaan 95% kemudian dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan terdapat peningkatan diameter glomerulus, penurunan lebar ruang Bowman serta penurunan persentase nekrosis sel tubulus proksimal pada perlakuan P1-P3 dengan dosis optimum yang dapat memperbaiki struktur histologis ginjal yaitu 385 mg/KgBB. Kesimpulan penelitian ini yaitu kombinasi ekstrak temulawak dan belimbing wuluh berpengaruh memperbaiki struktur histologis ginjal tikus yang terpapar paraquat.
Co-Authors Ade Pandi Adnin Laila Alyssa Meutia Fadiyah Andry Dian Alyssa Anggita Levi Astuti Annisa Anindyta Larasati Annisa Annisa Annisa Annisa Azalea Putri Dea Rahman Khairunnisa Desak Made Malini Dini Tasuruni Dita Aprila Khoirunnisa Dwi Rahmi Dwiki Danang Kuncoro Dyah Retnosari Emay Maulani Erri Noviar Megantara Ida Indrawati Indri Wulandari Indriani Sasmita Kabul Fadilah Kartiawati Alipin Keukeu Kaniawati Rosada Madihah Madihah Mia Miranti Mia Miranti Miranti Ariyani Mohamad Nurzaman Nadia Istiqomah Nia Daniati Hidajat Nurullia Fitriani Retnaningtyas Siska Dianty Rini Hafzari Rusdi Hasan Sadiah Djajasupena Salsabila Nurjihan Sri Rejeki Rahayuningsih SRI WULANDARI sunardi sunardi Supartini Syarif Supartini Syarif Suryana Suryana Sya Sya Shanida Syifa Yolanda Tatang Suharmana Erawan Teguh Husodo Tentani Buhti Amadea Tia Setiawati Titin Supriatun Tresna Wardhana Wawan Hermawan Yetty Yusri Gani Yolani Syaputri Yoyo Marten