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Journal : Journal of Chemical Learning Innovation

Analytical Method Optimization of H₂O₂ Derivatization Reaction Time for HPLC Analysis under Tropical Laboratory Conditions Dinalia; Sosidi, Husain; Ridhay, Ahmad; Hannan, Hannan; Khairuddin, Khairuddin; Satrimafitrah, Pasjan; Syamsuddin, Syamsuddin
Journal of Chemical Learning Innovation Vol. 3 No. 1 (2026): June
Publisher : Cahaya Ilmu Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37251/jocli.v3i1.3086

Abstract

Purpose of the study: This study aims to evaluate the suitability of the commonly reported 2-hour derivatization time for hydrogen peroxide (H₂O₂) determination using HPLC-PDA by assessing the formation behavior of triphenylphosphine oxide (TPPO) under tropical laboratory conditions. Methodology: The study employed an HPLC–PDA system (Shimadzu LC-20AD) with a reversed-phase C18 column (Waters X-Bridge, 4.6 × 150 mm, 5 µm). Derivatization of H₂O₂ with triphenylphosphine (Sigma-Aldrich) was performed using acetonitrile (Merck). The time-dependent kinetics of TPPO formation were evaluated based on chromatographic peak area monitoring. Data were processed using integrated instrument software. Main Findings: The TPPO peak area increased progressively with reaction time and approached a plateau at approximately 60 min, indicating near-complete derivatization under the studied conditions. No substantial increase in analytical response was observed at longer reaction times. Repeatability evaluation at 60 min showed acceptable analytical precision with %RSD below 2%, demonstrating that a shorter derivatization time was adequate for routine analysis. Novelty/Originality of this study: This study provides a context-specific evaluation of derivatization time as part of analytical method optimization in HPLC systems under tropical laboratory conditions. The findings demonstrate the importance of verifying derivatization parameters under specific working environments to improve analytical efficiency and ensure reliable chromatographic performance.
Co-Authors Abd. Rahman Razak Abd. Rahman Razak Abd. Rahman Razak Abustiana, Rahma Lolita Agriyani Marcelinda, Agriyani Aini Auliana Amar Aini Hoiriyah, Aini Alfian Noor Asniawati Asniawati Baehaqi Betzy Eppang Bryan Agung Rundubelo Darwin Darwin Debi Christa Ondagau Deddy Firman Didit Purwanto Dinalia Dinalia, Dinalia Djumidar Dwi Juli Puspitasari Dwi Juli Pusptasari Erwin Abdul Rahim Erwin Erwin Fatiah Riskah Fitrianingsih Mozin Gilang Almilda Hannan, Hannan Hardi Ys Hardi Ys Husain Sosidi I Nyoman Adi Putra Ian van Altena Indriani Indriani Indriani Indriani Indriani Indriani Isabella de Nadra Ivitri Susana Jaya Hardi Jusman, Jusman Khairuddin Khairuddin - Khairuddin Khairuddin Kurniawati Kurniawati Lindawati, Eka Magfira Magfira Mappiratu Mappiratu Mappiratu Mappiratu Marsela, Anggi Mirzan, Muhamad Moh. Mirzan Moh. Mirzan Mohamad Afdal Musafira Musafira Musdalifah Musdalifah Naser, Inda Hastuti N Ni Ketut Sumarni Ni Luh Sumitriasih Nov Irmawati Inda Nunuk H. Soekamto Nur Faidah Nur Istiqomah Nur Madinah Rizal Nur'ain Nur'ain Nurakhirawati Nurakhirawati Nurakhirawati Nurhaen, Nurhaen Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhalisa, Sitti Nurmila Nurmila Nurul Bima Khasanah NURUL CHOLIFAH Oktavian Mangela Parwati Parwati Pasjan Satrimafitrah Patrisia Marcevin Rengku Pratiwi Listyana Sareu Prismawiryanti Prismawiryanti Rahma Wati Rahmadani, Indah Ashari Ripdayana Ripdayana Riski, Mutiara Zalfa Ruslan Ruslan Ruslan Sabarwati, Sitti Hadijah Siti Helmyati Sitti Nur Halizah SYAIFUL BAHRI Syaiful Bahri Syaiful Bahri Syamsuddin Syamsuddin Syamsuddin Syamsuddin Syamsuddin Tjodi Harlim Ulayya Kasio Vivi Sasmita Winarsii, Dessy