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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis AQUATIC SCIENCE & MANAGEMENT JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Perikanan dan Kelautan The Journal of Experimental Life Sciences (JELS) Abdimas Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Journal of Food and Life Sciences Journal of Innovation and Applied Technology Jurnal Pembangunan dan Alam Lestari JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) EnviroScienteae Jurnal Biologi Tropis Jurnal Riset Akuakultur Samakia: Jurnal Ilmu Perikanan Musamus Fisheries and Marine Journal Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Environmental Pollution Journal Jurnal Ilmiah Sains Jurnal Kedokteran Hewan Journal of Fisheries & Marine Jurnal Perikanan dan Kelautan
Yenny Risjani
Department Of Aquatic Resources Management, Faculty Of Fisheries And Marine Science, Brawijaya University, Indonesia
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Journal : JOURNAL OF COASTAL DEVELOPMENT

 1 
PURIFICATION AND CHARACTERIZATION OF Aeromonas media KLU 11.16 CHITOSANASE ISOLATED FROM SHRIMP WASTE Ekowati Chasanah; Gintung Patantis; Dewi Seswita Zilda; Mahrus Ali; Yenny Risjani
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 1 (2011): Volume 15, Number 1, Year 2011
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Our previous study found that KLU 11.16, isolated from shrimp waste secreted chitinolytic enzymes. The crude enzyme was interesting since their chitooligosccharide was able to inhibit some pathogenic bacteria. In this study we report a purification and characterization of the chitosanase enzyme produced and the identification of the KLU 11.16. Purification of the enzyme was done two steps by ion exchange chromatography followed by gel filtration. Two out of 4 peaks from Gel Filtration step, i.e. fraction 16 and 33 were capable of hydrolyzing 100% deacetylated chitosan, indicating that both fractions contained chitosanase enzyme. The enzyme from fraction 16 had approximate molecular weight of 98.3 kDa. The enzyme worked optimally at temperature of 300C, and pH 6. Addition of Ca2+, Fe2+, K+, Na+ ions in the form of Cl2 salt and detergent Triton X-100 increased the enzyme activity, while Co2+, Mn2+ and Zn2+ ions in the same concentration decreased the enzyme acitivity. Addition of EDTA and SDS significantly decreased the enzyme activity. Molecular based identification revealed that KLU 11.16 was 99% similar to Aeromonas media.
Co-Authors A, Aulanniam Ach Khumaidi Adhimas Haryo Priyambodo Agoes Soegianto Agung Pramana Warih Mahendra Agung Pramana Warih Mahendra Aisyah, Jumrotul Aji Sutrisno Alfarisi, Muhammad Asnin Alfi Hermawati Alfi Hermawati Waskita Sari Alin Asyabil Andi Parenrengi Andi Parenrengi Andrzej Witkowski Anggraini, Rifka Rimbi Anisa Millah Taqiyyah Anisa Millah Taqiyyah Apriliyanti, Fisma Josara Arning W Ekawati Arsad, Sulastri Asep Awaludin Prihanto Asus Maizar Suryanto H Azis Husen, Azis Bagyo Yanuwiadi Defri Yona Dewi Cholifah Dewi Seswita Zilda Diana Arfiati Dr.Ir. Yunianta, DEA Dwi Rosalina Dwi Setijawati Ekowati Chasanah Elya Putri Pane Emmanuel Manangkalangi Endang Dewi Masithah Endang Yuli Herawati Ertikasari, Nuning Faiza Tawati Gesang Maulana Dwi Katmoko Gilang Drajat Maulana Gilang Drajat Maulana Gintung Patantis Happy Nursyam Iqbal, Muhammad Nurdzat Karimah Karimah Kurnia Rahmawati, Kurnia Kurnianingsih, Asmiyati Lawrence M. Liao Luky Sembel Maftuch Maftuch Maftuch Maftuch Mahrus Ali Mivida Febriani Moch Sasmito Djati Moh. Awaludin Adam Moh. Awaludin Adam Moh. Wildanul Jannah Mohammad Fadjar Mohammad Mahmudi Muhamad Firdaus Muhammad Dailami, Muhammad Muhammad Firdaus Muhammad Mahmudi Muhammad Mahmudi, Muhammad Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Triaji Muliawati Handayani Nanik Retno Wibowo Nunik Cokrowati Nunik Cokrowati Nunik Cokrowati Nur Aliya Nabila Zsalzsabil Nurul Mutmainnah Oktiyas Muzaky Luthfi Pane, Elya Putri Paricahya, Akhsan Fikrillah Patresna, Laelatul Nisa Philipus Musyeri Putra, Renanda Baghaz D.S. Rahardjo, Seto Sugianto Prabowo Rahmi Nurdiani Ramli Ramli Rifka Rimbi Anggraini Savira, Riza Seomarno Soemarno Siti Nur Tahirah Sri Andayani Sri Andayani Sri Andayani Sri Redjeki Hesti Mulyaningrum Sri Redjeki Hesti Mulyaningrum Sulastri Arsad Supriatin, Febriyani Eka TIWI NURJANNATI UTAMI Tongku N. Siregar, Tongku Wahidah Inayati Wibowo, Nanik Retno Wulan Cahya, Eka Yahya Yahya Yanuhar, Uun Yulianus Linggi Yuni Kilawati