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Journal : Jurnal Sains Teh dan Kina

Antioxidant activity of ten grades of Indonesia black tea (Camellia sinensis (L.) O. Kuntze) liquor Yayat Sudaryat; Mimin Kusmiyati; Citra Ratu Pelangi; A Rustamsyah; Dadan Rohdiana
Jurnal Penelitian Teh dan Kina Vol 18 No 2 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v18i2.70

Abstract

Tea is one kind of plant that is popular as a beverage. These plants contain antioxidant potential of flavonoids derived from polyphenol compounds. The research aimed to examine the antioxidant activity and to determine the con-tent of total phenols and total flavonoids from ten grades of Indonesian black tea liqour. Antioxidant activity was measured by the method of free radical scavenging by DPPH, total phenol content measured by Follin-Ciocalteu method, and total flavonoid content was measured by the method of AlCl3. The results of this research showed that The highest total phenol content contained in Dust III with 225,80 mgGAE/100 g and the lowest content is BTL with 111,26 mgGAE/100 g. The test results showed that BBL has the highest total flavonoid content with 0,151 mg/g, while the lowest is BTL flavonoid with 0,086 mg/g. Meanwhile the highest antioxidant activity of ten grade of Indonesian black tea infusion obtained by Dust I, with IC50 97,00 μg/ml. Meanwhile the lowest antioxidant activity with IC50 178,56 μg/ml was obtained by BTL.
Antioxidant activity, phenol total, and flavonoid total of green tea (Camellia sinensis (L.) O. Kuntze) from three West Java tea estate Mimin Kusmiyati; Yayat Sudaryat; Isti Agnia Lutfiah; Ardi Rustamsyah; Dadan Rohdiana
Jurnal Penelitian Teh dan Kina Vol 18 No 2 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v18i2.71

Abstract

Green tea has bioactive component, it is polyphenol which has strong antioxidant. As the largest component of polyphenol, flavonoid is very effective as an antioxidant. The purpose of this study was to determine antioxidant activity, phenol total and flavonoid total in green tea from three different estates, they were Cikajang Kab. Garut, Taraju Kab. Tasikmalaya, and Ciwidey Kab. Bandung. The results of this study show that seven samples have good antioxidant activity, but green tea which came from Cikajang (P-IRT No. 810320501698) has the highest IC50 grade it was 21,44 μg/ml. Beside that samples from Cikajang (P-IRT No. 810320501698) has highest total phenol content it was 334,68± SD 0,89/100 g samples and also has the highest total flavonoid it was 0,34 mg Kuersetin/g samples ± SD 0,21. On the contrary, green tea which has the lowest IC50 grade came from Taraju, the grade was 28,03 μg/ml, the samples has the lowest total phenol content it was 208,91± SD 4,47 mgGAE/100 g, and also has the lowest total flavonoid it was 0,207 mg Kuersetin/g samples ± SD 0,00.
Antibacteririal avtivities of Bacillus cereus and Shigella dysenteriae of white tea extract in several variation of solvents Asri Widyasanti; Andita Mega Priantiwi; Dadan Rohdiana
Jurnal Penelitian Teh dan Kina Vol 19 No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i1.81

Abstract

Tea is used in the food, cosmetic, and herbal drug industries. White tea is a kind of tea containing a relatively high polyphenol content compared to other kind of tea, and thus has the potential of being used as a natural antibacterial. The objective of this research was to study the antibacterial activity of of  white tea extract in several variation of  13 % (w/v) concentration of solvents to the activity of gram-positive bacteria (Bacillus cereus) and Gram-negative bacteria (Shigella dysenteriae). Laboratorium experimental method was used in this descriptive study. The antibacterial activity study was comprised of estimating inhibition area and total of bacterial growth. The results revealed that white tea extract inhibited bacterial growth, indicating that white tea extract from the n-hexane, ethyl acetate and 96% ethanol solvents could be developed as an antibacterial material. The diameter of the inhibition area to Bacillus cereus bacteria for n - hexane , ethyl acetate, and  ethanol 13 % (w/ v)  solvents were 0.6 mm, 10.5 mm and 1.75 mm, respectively; while for Shigella dysenteriae bacteria were 0,5 mm, 9,86 mm  and 10,6 mm, respectively. The total growth of Bacillus cereus bacteria for n-hexane, ethyl acetate, and ethanol 13 % (w/v) solvent were 1.3 x 107, 6.0 x 106, and  1.1 x 104 CFU / ml , and of Shigella dysenteriae bacteria were  1.0 x 107,  6.5 x 106 and 4.6 x 106 CFU / ml, respectively.
Optimization and characterization of green tea polyphenol extract from various solvents Shabri Shabri; Dadan Rohdiana
Jurnal Penelitian Teh dan Kina Vol 19 No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i1.82

Abstract

Tea has a high content of polyphenols with potent antioxidant activity and beneficial for health. Availability of polyphenols extract is still very limited. The aim of this study to optimation and characterization of green tea polyphenol extract base on some solvents. Parameters analyzed were polyphenol content and yield of some extraction process conditions. This result showed that extraction by the ratio of tea and solvent was 1 : 15 b/v, extracted by acetone 70% solution in temperature 600C for 15 minutes obtain polyphenol content of 53,30% b/b and total yield of 40,17% b/b.
Inhibitory activity of Escherichia coli by type of teas and its liquors Dadan Rohdiana; Dede Zainal Arief; Arista Budiman
Jurnal Penelitian Teh dan Kina Vol 16 No 1 (2013)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v16i1.86

Abstract

Study of analysis polyphenol content on tea, liquor, and its activity as antibacterial on Escherichia coli has been done conducted in Laboratory of Research, Department of Food Technology, Pasundan University, and Laboratory of Chemistry, Department of Chemistry, Faculty of Mathematic and Natural Science, Padjadjaran University. The result of this research showed that green tea sample has the highest polyphenol content, i.e. 23,18% followed orthodox and CTC (crushing tearing curling) black tea samples i.e. 14,23%  and 13,93% respectively. Activity of inhibitory on E. coli showed that ratio tea and water 2 : 50 w/v has the best inhibition i.e. 117,71 mm2.
(1,1-Diphenyl-2-Picrylhydrazyl) free radical scavenging activity of white tea base on temperature and period of brewing Dadan Rohdiana; Dede Zainal Arief; Mamay Somantri
Jurnal Penelitian Teh dan Kina Vol 16 No 1 (2013)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v16i1.87

Abstract

Research of 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) free radical scavenging activity has been done conducted on Laboratory of Quality Assesment, Research Institute for Tea and Cinchona, and Laboratory of Chemistry, Chemistry Department, Faculty of Matematic and Natural Science, Padjdjaran University. The result showed that among four white tea from different estates, total polyphenol content on Gambung White Tea has the highest. Total polyphenol content of Gambung white tea i.e 25,52%. Brewing process on 95oC for 9 minutes obtained the highest polyphenol content on tea infusion i.e. 6,01% and the lowest EC50 i.e. 35,41 µl/ml. This research showed that presence of positive correlation between total polyphenol and antioxidant activity base on EC50. The correlation value in this research i.e. 0,9066.
Analisis kandungan teaflavin dan tearubigin pada teh hitam yang diolah melalui metode pelayuan kimia bertahap Shabri Shabri; Dr. Dadan Rohdiana; Hilman Maulana; Sugeng Haryanto; Muhammad Iqbal Prawira-Atmaja
Jurnal Penelitian Teh dan Kina Vol 21 No 1 (2018): Jurnal Penelitian Teh dan Kina
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v21i1.142

Abstract

The instability of tea shoots supply from the plantation to the factory was often a problem. In the flush season where tea shoots are abundant, an overcapacity often leads to the uncontrollable withering process. This problem was the cause of the decreased quality of black tea. Theaflavin (TF) and Thearubigin (TR) were chemical substances that positively correlated to the black tea quality. This research conducted to characterize TF and TR quality in two-stages chemical withering. This research treatment consists of two treatments, namely treatment A (plus season), treatment B (minus season), and one control/routine production (treatment C). Treatment A and B used 25 kg of fresh tea shoots to be withered, the first stage was to wither the leaves until it reached 60%, 65%, and 70% of water content. The second stage was to blended those leaves at the first stage and withered it until it reached about 54-56%. For treatment A and B, the enzymatic oxidation (fermentation) time used 90 minutes. Treatment C used 25 kg of fresh tea shot that processed as normal routine production of black tea orthodox-rotor vane process, with enzymatic oxidation time 110 minutes. Every treatment was repeated 3 times. Treatment A produced TF of 0,97% d.b and TR of 15,39 d.b. (ratio 1:15,97). And treatment C produced TF of 0,92% d.b and TR of 13,69% d.b. (ratio 1:14,82). It was concluded that two-stage chemical withering was able to maintain the chemical and organoleptic qualities of black tea.
Karakteristik pelet kayu dari limbah pangkasan teh berdasarkan besaran partikel Sugeng Harianto; Muhammad Iqbal Prawira-Atmaja; Shabri Shabri; Hilman Maulana; Dr. Dadan Rohdiana; Achmad Imron Rosyadi
Jurnal Penelitian Teh dan Kina Vol 21 No 1 (2018): Jurnal Penelitian Teh dan Kina
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v21i1.143

Abstract

Biomass waste potential from tea pruning was high, however the utilization of the waste still low. Forming pellet from the waste was one of means for utilizing it to have value-added. Pelletization is a process of compressing the material to increase density value, calorific value, and to uniform the particle size. The method in tea pruning waste pelletization used because the waste is consists of dry twigs and leaves so it was easier in reducing the particle size as pellet raw material. This research purpose was to identify the wood pellet characteristics of various particle size from tea pruning waste, consisted of water content, ash content, and wood pellet density against calorific value produce. The material used in this research was tea pruning waste available all years in the experimental plantation of Reserach Institute for Tea and Cinchona Gambung that has been dried for three days. Pelletization process starts with reducing the tea pruning waste particle using Chopper machine to acquire dust particles. Afterward, those dust was separated into three treatments, which are, passed sieve number 7 mesh, 14 mesh, and 18 mesh. Calorific value produced from the wood pellet was the main factor from all pelletization process, the result show that the dust passed sieve number 18, which is the smallest particle size, has the highest calorific y value produced in the amount of 4.431 cal/g
Karakteristik nanopartikel ekstrak teh hijau dengan metode nano milling dan nano spray Shabri Shabri; Dr. Dadan Rohdiana; Hilman Maulana; Sugeng Haryanto; Muhammad Iqbal Prawira-Atmaja; Rachmat Mauludin; Muhammad Insanu; Asep W. Perdana
Jurnal Penelitian Teh dan Kina Vol 21 No 2 (2018): Jurnal Penelitian Teh dan Kina
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v21i2.146

Abstract

Nanoparticles for raw materials of phytopharmaca is a technology used to be able to increase the effectiveness and efficiency of phytopharmaca in its absorption as a therapeutic agent. This study useds steamed green tea polyphenol extract as raw material with two nanoparticle methods, first nano milling by Planetary Ball Milling (PBM) and the second using encapsulation method with Nano Spray Dryer (NSD). The characteristics of nanoparticles were then analyzed using Particle Size Analyzer (PSA) and X-Ray Difraction (XRD) to determine the size, size distribution, and type of crystals formed by the two methods. The results of PBM had not been able to meet the criteria for nanoparticles because they had a particle size that is >1 µm, whereas the NSD method produced 554.7 nm nanoparticles with an intensity of 86.9%. And the results of the XRD analysis showed that the two methods of making nanoparticles resulted in amorphous particles >50%.
Karakteritik sifat alir bubuk teh hijau yang diproses dengan metode penepung berbeda Mukhammad Iqbal Prawira-Atmaja; Sugeng Haryanto; Hilman Maulana; Shabri Shabri; Dr. Dadan Rohdiana
Jurnal Penelitian Teh dan Kina Vol 21 No 2 (2018): Jurnal Penelitian Teh dan Kina
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v21i2.147

Abstract

Recently, the public interest in green tea powder is increasing. Green tea powder is produced through the process stage: withering in hot steam, drying, and reducing size. The size reduction process on green tea powder is an important step because it will affect the characteristics of the powder flow properties. This study aims to determine the flow properties of green tea powder processed by different milling method. The milling method used are disc milling (DM) and stone milling (SM). Green tea powder was analyzed including moisture content, particle size distribution using dynamic laser scattering method, determined of bulk density (ρb), and tapped density (ρtap) to find out the Carr Index (CI) and Hausner Ratio (Hr). In addition, the lightness of color and shape and morphology of green tea powder were observed with SEM. The results showed that green tea powder produced from different milling methods have a Carr Index of 15 with “Good” flow properties. DM method produced darker green tea powder with L value (lightness) 49.91 and larger particle size (D50: 38.6 µm) compared with green tea powder produced by the SM method. Determination of flow properties of green tea powder can be applied for the utilization and development of food products and pharmaceutical products based on green tea powder.
Co-Authors A Rustamsyah A Rustamsyah, A Achmad Imron Rosyadi Adang Firmansyah Akhmad Endang Zainal Hasan Amalia Rahma Ananda, Marsha Radhia Andita Mega Priantiwi Andita Mega Priantiwi, Andita Mega Andri Agustav Wirabudi Angga Rusdinar Anik Setiawati Anik Setiawati, Anik Ardi Rustamsyah Ardi Rustamsyah Arista Budiman Arista Budiman, Arista Asep W. Perdana Asri Widyasanti Awaliah, Nisa Azmi, M. Haikal Beauty Azhary Bina Lohita Sari Budi Setiawan Cece Sumantri Citra Ratu Pelangi Citra Ratu Pelangi, Citra Ratu Clara Meliyanti Kusharto Darningsih, Sri Dede Zainal Arief, Dede Zainal DIMAS ANDRIANTO Dinda Nuraini Maulfida Dini Febrianty Dong Ho Lee Dwi Putra, Januar Hafiz Dwiyan Nugraha Dytha Andri Deswati, Dytha Andri Ekatama, Novriana Evy Damayanthi Faillah, Umi Lutfiatul Fani Fauziah Farddani, Chintya Listiarsi Fauzan, Fajar Akmal Fauzi, Ridho Hadi Riyadi Hanipah, Anida Hanny Hafiar Hasna Zakiyah Heddy Julistiono Heri Aryadi Hidayat, Taufik Septiyan Hijaya, Andi Hilman Maulana Hilman Maulana Hilman Maulana I Gusti Bagus Wiksuana Ida Maryam Ika D. Mayangsari Ikrawan, Yusep Isti Agnia Lutfiah Isti Agnia Lutfiah, Isti Agnia Komar Ruslan Wirasutisna Komar Ruslan Wirasutisna Kristin, Julia M Iqbal Prawira-Atmaja M. Iqbal Prawira-Atmaja Mamay Somantri Mamay Somantri, Mamay Marlinda Adham Maryam, Ida Mimin Kusmiyati, Mimin Miranti Putri Utami Muhamad Alvin Adriyan Muhamad Insanu Muhammad Insanu Muhammad Iqbal Prawira-Atmaja Mukhammad Iqbal Prawira-Atmaja Mukhammad Iqbal Prawira-Atmaja Murdijati Gardjito Novi Susanti Nuni Yunita Nuni Yunita, Nuni Nurlita, Selvi Nurzanah, Fitri Prawira-Atmaja, Mukhammad Iqbal Rachmat Mauludin Rahayu, Dien Puji Rendy Munadi Rimbawan , Rimbawan Rimbawan Rina Martini Rohayati Suprihatini Rosialani, Resa Rosyanne Kushargina Rosyanne Kushargina Sahara, Putri Siti Sari Agustin Shabri Shabri Shabri Shabri Shabri Shabri Shabri Shabri Shabri Shabri, Shabri Siti Hajar Siti Hajar Sri Anna Marliyati Sri Raharjo SUGENG HARIANTO Sugeng Harianto Sugeng Harianto Sugeng Haryanto SUHARYANTO SUHARYANTO Syumillah Saepudin Tri Halimah Tri Panji Yayat Sudaryat Yunita Al-Azzahra Yusianto Yusianto Yusianto Yusianto, Yusianto Zahra, Nur Adni Azkia Zanil Musa