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Journal : Journal of Bio-molecule Research And Engineering

Phylogenetic analysis of DENV-1 isolated in Surabaya, Indonesia Rahmafitria, Fistara Lesti; Mahfudhah, Dzikra Nasyaya; Sucipto, Teguh Hari; Herdyastuti, Nuniek; Anggarani, Mirwa Adiprahara; Fauziyah, Shifa; Damayanti, Mamik
Journal of Bio-Molecule Research and Engineering Vol 1 No 2 (2022)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v1i2.41463

Abstract

Dengue virus (DENV1-4) belongs to the Flaviviridae family, which is transmitted by the Aedes mosquito vector and is the main cause of dengue fever and dengue hemorrhagic fever. Since one of the DENV serotypes, DENV1, has become an endemic known to be circulating worldwide, including in Indonesia, it becomes necessary to carry out molecular epidemiological research using phylogenetic analysis with two methods, neighbor-joining (NJ) and UPGMA. This study aims to analyze the DENV-1 relationship and obtain information regarding the differences between those methods, including the level of accuracy. This study used one DENV-1 sequence isolated in Surabaya, aligned with similar sequences on the GenBank. The results showed two comparisons. First, in the NJ method, the DENV-1 sequence samples in Surabaya with branch length 0,000 were similar to the DENV-1 in Malaysia, and Singapore, with branch lengths 0,000; 0,002;. which belong to Genotype 1. The UPGMA method resulted in the DENV-1 sequence in Surabaya with branch length 0,000 were similar to the DENV-1 in Malaysia with with branch length 0,000, which belong to Genotype 1. Second, their level of accuracy, which is in the NJ method, the construction of phylogenetic trees is based on periodic evolutionary times. In contrast, UPGMA assumes that each sequence is found at the same evolutionary time, which makes this method less accurate than the NJ method. We can conclude that the construction and analysis of the phylogenetic tree of the DENV1 sequence isolated in Surabaya have higher similarity and accuracy using the NJ method.
Elimination of Dengue Virus with Antiviral Compound and Appropriate Technology Farihah, Neni Isna; Wijayanti, Alvia Rachma; Sucipto, Teguh Hari; Putri, Deva Permata; Ihsan, Anaqi Syaddad; Fauziyah, Shifa; Saputri, Ratih Dewi; Damayanti, Mamik
Journal of Bio-Molecule Research and Engineering Vol 2 No 1 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i1.44803

Abstract

Infection with the dengue virus by the Aedes aegypti mosquito vector is in the form of dengue hemorrhagic fever (DHF), which can cause a decrease in platelets and even death. The parasitic drug niclosamide, which is effective against dengue virus serotype 2 (DENV-2) is used to prevent further dengue virus infection. Many tests were carried out using inhibitors such as doxorubicin (SA-17), glycoside inhibitors in the form of deocynojirimycin (DNJ) and castanospermine (CSP), carbohydrate-binding agents (CBA), and the use of heparan sulfate aimed at inhibition of the adsorption process and replication process, as well as improper protein folding to prevent the conformation of virus merger. The elimination process can also be carried out using antiviral compounds found in the leaves of Psidium guajava and Carica papaya, which have inhibitory activities of 92.6% and 89.5%, respectively; propyl gallate, with a percent inhibition of dengue virus envelope protein serotype 2 of 53-9.85%; isobutyl gallate, with CC50 values of 167.19 g/mL and an inhibitory value (IC50) of 4.45; Cissampelos Pariera Linn methanol extract, with progressive inhibition as the Cipa extract concentration increased with an IC50 value of 6.1μg/ml Preventive methods are also carried out in several ways, namely by utilizing hydrophobic liquid in the form of silicone oil (low-viscosity polydimethylsiloxane, or L-PDMS), the use of eave tubes in home tubes inserted with insecticides, and utilizing ultrasound with a frequency of 100 kHz and 90 dB to repel mosquitoes carrying dengue virus vectors.
Co-Authors Adiana Mutamsari Witaningrum Adisti, Najeelina Putri Akbar, Agam Fauzie Aksono HP., Eduardus Bimo Amalia, Nurul Afifah Antari, Ratna Devi Apriliawan, Vian Dito Aquaresta, Febriana Arroikhaanunnisa, Dini Asri Nurdiana, Asri Bambang Setiabudi Budi Utomo DAMAYANTI, MAMIK Endyka Erye Frety Fachryandini, Nofita Fardzanela Suwarto Farihah, Neni Isna Frida Kistiani Hanifah, Adiba Hasna Hapsari, Nafisah Nurul Hariyono Hariyono Harlinda, Nadya Meta Hartono H, Hartono Hartono Hartono Heriqbaldi, Ayurveda Zaynabila Hery Suliantoro Husna, Arifka Farah Ihsan, Anaqi Syaddad Karuniawan, Muh.Fauzi Alfani Khalisha, Mujahidah Kurniawan, Muhammad Ridho Hafid Kusumawati, Nema Oryza Leo Yosdimyati Romli M. Agung Wibowo M. Agung Wibowo Mahfudhah, Dzikra Nasyaya Mirwa Adiprahara Anggarani Moh Nur Sholeh Moh. Nur Sholeh Muthu, Pandaram Naw, Sin War Nugroho, Salsabila Bestari Nuniek Herdyastuti Nur Syamsiatul Fajar, Nur Syamsiatul Nurjihad C, Fajar Paramarta, Made Bayu Angga Praba, Venansya Maulina Putri, Deva Permata Putri, Melvanda Gisela Putri, Natasya Ariesta Selyardi Rahmafitria, Fistara Lesti Ramadhani, Putri Karisma Ramadhani, Zulfa Nurfitri Raniasari, Nisrina Nabila Ratih Widiastuti Razan, Rafi Alfian Sa'adi, Ashon Saputri, Ratih Dewi Sentosa, Khalid Al Rasyid Setiabudi Bambang Siahaan, Paulus Parholong Sin War Naw Sri Pantja Madyawati, Sri Pantja Sudaryani, Sudaryani Suwarto, Fardzanela Tan, Chin Xuan Teguh Hari Sucipto, Teguh Hari Tessari, Chelssi Gloria Undayani Cita Sari Widya, Alicia Margaretta Widyasari, Sheny Wijayanti, Alvia Rachma Yaqub, Muhammad Ardhiansyah Sofyan