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Journal : JURNAL BIOLOGI PAPUA

Aktivitas Enzim Amilase Isolat Bakteri Amilolitik dari Tepung Sagu Basah dan Lingkungan Tempat Penyediaannya Secara Tradisional di Jayapura Suprapto Surapto; Tri Gunaedi; Basa T. Rumahorbo
Jurnal Biologi Papua Vol 6, No 2 (2014)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.415 KB) | DOI: 10.31957/jbp.457

Abstract

The study about the activity of the enzyme amylase from amylolytic bacterial isolates from wet sagoo starch and  its traditional provision environment had been done in Jayapura. The purposes of this study were to determine the activity of amylase enzyme and to identify the bacteria isolated from wet sagoo starch and its processing environment in Jayapura district. The method used was an experimental laboratorium in which isolation of amylolytic bacteria was performed by using nutrient agar medium with 1% soluble starch on spreed pour plate method. The enzyme activity was detected with 0.2% iodine in 2% potassium iodide which were able to form a clear zone. The protein content of the crude enzyme extract was determined by the Bradford method using bovine serum albumin (BSA). Amylase enzyme activity was determined by the formula: DUN/ml = [(R0-R1)/R0] [dilution factor] DUN/ml (dextrinizing units per ml). The results showed that there were 15 isolates amylolytic bacteria. Four (4) bacterial isolates have amylolytic power of more than 30 mm. The amilase activity of amylolytic bacterial of all  isolates were quite high: which were 35 577, 18 903,  32 106 and 46 600 U/mg for SU4, SU13, SU23 and SU40 respectively. The identification of isolates indicated that the three isolates are members of the Bacillus cereus ATCC 14 579 types with a similarity value of 71.70% to 81.10%, and one isolate is Bacillus subtilis ATCC 6501 members with a similarity value of 94.30%. Keywords: Amylolytic bacteria, amylase activity, characterization, sago flour.
Status Mutu Kualitas Udara Ambien Pada Beberapa Kota Besar di Papua Menggunakan ArcGIS Indah D. Setyowati; Auldry F. Walukow; Basa T. Rumahorbo; Johnson Siallagan; Novita Medyati
Jurnal Biologi Papua Vol 15, No 1 (2023)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jbp.2701

Abstract

The increase in population in recent years has affected the utilization of facilities and infrastructure, including the use of vehicles. For the past 10 years, the population in Papua has increased by around 4.13% per year, resulting in the need for daily mobility to increase. The high mobility demand in Papua could affect the increase in pollution, thus affecting the air quality. Exposure to pollutants in ambient air and environmental noise in the long term will have an impact on human health. Therefore, in this study, we analyze the current conditions of air pollution parameters in several major cities in Papua, such as Jayapura, Merauke, Sentani, and Timika. The method used is a survey. Environmental parameters measured were air content of SO2, NO3, PM 10, PM 2.5, HC, O3, CO, dust, Pb, noise, and The Air Pollutant Standard Index (ISPU) value. Observational data obtained were analyzed and displayed using the ArcGIS program. The parameters for determining the location of sampling points are densely packed highway areas, industrial areas, residential areas, and offices. The results of the study indicated that the ambient air quality in all study sites is below the required quality standard. While the results of environmental noise that exceed the highest quality standards located in specifics area on each study site are as follows, Merauke at PLTD Merauke I PT. PLN Persero with 81.24 dB(A), Sentani at Sentani City Square Roads with 70.2 dB (A), Mimika at Perumahan Pemda SP-2 with 65.30 dB(A), and Sentani at Kantor Bupati Jayapura with 64, 54 dB (A). However, the ISPU value for pollutant substances is still in the range of 0-50, which means that the air quality category is very good to carry any outdoor activities, and does not have any negative impact on humans, animals, or plants.Key words: air quality; noice; ISPU; Papua.
Co-Authors . Ngatinah, . . Risakotta, Tesalonika K A.A. Ketut Agung Cahyawan W Abrauw, Rudolf Agamawan, Lalu P. I. Alfred A Antoh Alianto , Apriani Herni Rophi Auldry Fransje Walukouw B Fitriya, Winda Ade Baigo Hamuna Baigo Hamuna Baigo Hamuna Battu, Yudistira S. BENEDICTA ENDAWATI TITIRLOLOBY Betinia Susanti Runggamusi Boli, Elisabert Bre Chandra I. Lumban Gaol Daniel Z.K. Wambrauw Diana M. Abulais Edoward Krisson Raunsay Efray Wanimbo ELVI RAPILUS Ervina Indrayani Felycitae Ekalaya Appa Friyuanita Lubis Hamzah, Hardi Hardi Hamzah Hehanussa, Kedswin Gerson Henderina J. Keiluhu Hisya, Muhammad Husain Husain Husain Husain Indah D. Setyowati Indrayani, Elvina Janpiter Manalu Jesajas, David R. John Dominggus Kalor John Dominggus Kalor, John Dominggus Johnson Siallagan JOHNSON SIALLAGAN Kalvin Paiki Karisoh, Gabriela O. Korinus Rejauw Kristoper A. A. Manalu Kuddi, Bobi F. Lainsamputty, Judith Lani Kawengian Linda Hetty Yanne Fatahan Lisiard Dimara Lolita Tuhumena Maklon Warpur Manalu, Janviter Manalu, Yanviter Mandey, Vera Kostansie Min Adii NGATINAH NGATINAH Nicea Roona Paranoan Novita Medyati Nur Fadilah Bakri NURCAHYA NURCAHYA Octolia Togibasa Paranoan, Nicea Roona Paulangan, Yunus P. Payapo, Muhammad Zia Ulhaq Pirhel, Pirhel Pisi Bethania Titalessy Popi Ida Laila Ayer Puji Utama, M. Wahyu Ramadei, Yohana Rejauw, Korinus Retno Hadisutjipto Rosye H.R. Tanjung Rumbiak, Kristhopolus K. Salhuteru, Stefani T Sara Umbekna Saramuke, Dina Mathilda Sialagan, Johnson Snahan, Amida Laurita Suprapto Surapto Tomasila, Leopold A. Tri Gunaedi Tri Gunaedi Tuhumena, Lolita Constantia Umbekna , Sara Umbekna, Sara Virman Virman Walukouw, Auldry F Wambrauw, Daniel Z.K. Way, Jufentus Ronaldo Yohana Adriana Korwam, Yohana Adriana Yulindra M. Numberi