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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Science and Technology Indonesia Majalah Kesehatan FKUB Indonesian Journal of Kidney and Hypertension JIMEK : Jurnal Ilmiah Mahasiswa Ekonomi Heart Science Journal CRJIM (Clinical and Research Journal in Internal Medicine) Jurnal Jeumpa Narra J Jurnal Klinik dan Riset Kesehatan Jurnal Penyakit Dalam Indonesia Jurnal Kesehatan Deka in Medicine
Nur Samsu
Brawijaya University, Dr. Saiful Anwar Hospital, Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Role of Antibody Anti-AGE in the Expression of Nephrin and RAGE on Primary Glomerulus Cell Exposed with AGE Salam, Rudy; Lyrawati, Diana; Samsu, Nur
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.03

Abstract

Upregulation of nephrin expression occurs at the early stage of nephropathy and decrease in the period 6 months on hyperglycaemic condition. Nephrin is associated with the initial stage of the loss of the permeability barrier in diabetic nephropathy. Interaction AGE-RAGE increases angiotensin II on Renin Angiotensin-Aldosterone System (RAAS) and activation of protein kinase c (PKC) which induce alterations in nephrin mRNA expression. Alterations of nephrin expression induces transformation of slit membrane structure and the permeability changes at the glomerular filtration barrier. Anti-AGE vaccination once may cause the changes of nephrin and RAGE expression and can prevent progression of diabetic nephropathy. This study used primary glomerulus cell culture obtained from renal of Wistar mice aged 3 months, weighting 200-300 grams that consist of negative control group that exposed to BSA 100 µg/ml, positive control group that exposed to AGE-BSA 100 µg/ml, treatment group 1 that exposed to polyclonal anti-AGE 5 µg/ml and AGE-BSA 100 µg/ml and treatment group 2 that exposed to monoclonal antibody anti-CML 5 µg/ml and AGE-BSA 100 µg/ml. Paired t-test with a 0.05 level of confidence results showed that there were significant differences in level of RAGE expression among experimental groups with control groups. Administration of polyclonal antibody decreased RAGE expression among negative control (p=0.188). but not in positive control (p=0.000). In contrast to monoclonal anti-AGE antibody, RAGE expression did not differ significantly compared to negative control but significant than positive control. Administration of monoclonal anti-AGE antibody inhibited increasing of nephrin expression compared to negative and positive control (p=0.73; 0.125). In conclusion, this study suggested that administration of polyclonal and monoclonal anti-AGE antibody could inhibit increasing of RAGE and nephrin expression in glomerulus primary culture that exposed to AGE which is expected to prevent the progression of diabetic nephropaty.
Advanced Glycation End Products (AGEs) Antibody Protects Against AGEs-induced Apoptosis and NF-ĸB p65 Subunit Overexpression in Rat Glomerular Culture Adianingsih, Oktavia Rahayu; Lyrawati, Diana; Samsu, Nur
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.08

Abstract

Advanced glycation end products (AGEs) have been thought to be a major cause of diabetic nephropathy (DN). The mechanisms underlying the involvement of AGEs antibody in diabetic nephropathy are not fully understood. The present study was designed to investigate the protective effect of AGEs antibody on AGEs-induced glomerular damage. Isolated glomeruli were pre-incubated either with 10 µg/mL polyclonal anti-AGEs antibody (AGE-pAb) or monoclonal anti-Nɜ -carboxymethyl-lysine antibody (CML-mAb) as a model of AGEs antibody to block interaction of AGEs with receptor for AGEs (RAGE) and incubated afterwards either with 100 µg/mL bovine serum albumin (BSA) or AGE-modified bovine serum albumin (AGE-BSA) for 48 h. Annexin V/nephrin doublestaining was performed to determine apoptosis. Using immunofluorescence, we found that administration of AGE-BSA not only significantly increased glomerular cells apoptosis and nuclear factor kappa B (NF-ĸB) p65 expression, but also reduced expression of nephrin, an important structural and signal molecule of podocytes slit diaphragm. Blocking the interaction of AGE-RAGE with AGEs antibody significantly protected glomerular cells from AGEs-induced apoptosis and NF-ĸB p65 overexpression. We found that AGE-pAb conferred superior protective effect compared with CmL-mAb for the same reduction in apoptosis and NF-ĸB p65 expression. In sharp contrast, CmL-mAb led to preserve expression of podocytes nephrin better than AGE-pAb. These results demonstrate that the antibody against AGEs may be beneficial for preventing the glomerular damage in DN.
Co-Authors ACHMAD RIFAI Achmad Rudijanto Akhmad Sabarudin Albaar, Thoha M. Arie Srihardyastutie asihanti rosita ferdiana Aulanni’am Aulya, Justika Usmadhani Baridwan, Fakhri Camoya Gersom Dela, Jamaludin A. Dewi, Ratih TK. Diana Lyrawati Didi Candradikusuma Dwinata, Michael Fachrureza, Mochamad Fahmi, Ahmad Luthfi Fajar, Jonny Karunia Farahdina, Farahdina Fatimatuz Zahroh, Fatimatuz Fiddaroini, Saidun Fredo Tamara, Fredo Friatna, Yudha Gandung Satriyono, Gandung Hani Susanti, Hani Heri Sutanto Huda, Fariz N. Ida Wahyuni Indiastari, Dewi Jack Roebijoso Jonny K. Fajar Kosasih, Angelia Kusuma, Yeziga Enggar Matondang, Asri Rizqi Mutia, Tari Ni Luh Tantri Niniek Budiarti B Novitasari, Adinda Dian Nurike S Mudjari, Nurike S Oktavia Rahayu Adianingsih Pratama, Satya Putra, Riyantono Putri, Irawati E. Rifa'i, Achmad Rizaliansyah, Ferdian Roja’i, Abdul Munir Rosandy, Kharisma Ogit Rudy Salam, Rudy Salsabila, Isadora Evani Saraswati, Dara Sari, Fitria R. Susanto, Agung Suseno, Aryo Sutrisno, Muhammad A. Tidel, Caesar Tupamahu, Alvira R. Waafi, Affa Kiysa Wicaksono, Ramadi S. Winoto, Eden Winoto, Eden Suryoiman Wursito, Wursito Zuhri, Mohammad harisudin