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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Indonesian Journal of Electronics and Instrumentation Systems Jurnal Ilmu Dasar Microbiology Indonesia BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Global Medical and Health Communication UNEJ e-Proceeding Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI Warta Pengabdian Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Jurnal Riset Biologi dan Aplikasinya Jurnal Bioteknologi & Biosains Indonesia (JBBI)

Kartika Senjarini

Biology Department, Faculty Of Mathematic And Natural Sciences, University Of Jember

Author-ID : 375743

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Dentistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Nursing Physics Public Health Veterinary

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Analisis Hasil Elektroforesis DNA dengan Image Processing Menggunakan Metode Gaussian Filter Khairul Anam; Widya Cahyadi; Ihsanul Azmi; Kartika Senjarini; Rike Oktarianti
IJEIS (Indonesian Journal of Electronics and Instrumentation Systems) Vol 11, No 1 (2021): April
Publisher : IndoCEISS in colaboration with Universitas Gadjah Mada, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijeis.58268

DNA gel electrophoresis plays an important role in the development of science. However, the process of manually analyzing DNA size is still relatively difficult, time-consuming, and often results an error. This study proposed electrophoresis process using image processing with Gaussian Filter method. Gaussian Filter is used to improve the quality of the image which makes the image clearer. The method was applied using python programming and then embedded into Raspberry pi 3 module. This modul processed images taken by Raspberry pi V1 camera. To realize these taken images, tracking mouse was used. All the images which had been processed were displayed on LCD touchscreen 5 inch. The result shows that the study using Gaussian Filter indicates good performance. This is proved by the lowest error percentage of 0,20% . In addition, compared to previous studies, the largest error percentage is still relatively smaller at 12.41%.

Imunogenic Protein of Salivary Gland from Anopheles sundaicus Yunita Armiyanti; Moh Mirza Nuryady; Sugeng Setyo Utomo; Teguh Wahju Sardjono; Loeki Enggar Fitri; Kartika Senjarini
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Malaria is still a major problem for developing countries, including Indonesia. One approach to overcome this disease is prevention by vaccination. However, there is still no effective malaria vaccine that is applicable. The ideal malaria vaccine is a combination vaccine that can prevent the pre-erythrocytic cycle, the erythrocytic cycle and transmission process. Salivary vector-based vaccine has the potential to be developed as a malaria vaccine because it can prevent transmission process and also decrease the morbidity of the disease. Saliva from Anopheles contains vasomodulator and immunomodulatory components, that are required in the blood feeding process, but in the same time it could enhance the transmission of the malaria parasite. If the component in the salivary vector can increase pathogen infection, then vaccinating the host with its anti-substances can control the transmission of pathogens (Transmision Blocking Vaccine). Anopheles sundaicus is an important vector of malaria in coastal areas of Java, Bali, Sumatra, Kalimantan and West Nusa Tenggara islands. Repeated exposures of Salivary Gland Extract (SGE) from this vector have been proven to be able to decrease parasitemic rates in mouse model for malaria in our study. The objective of this research is to determine and localize the immunogenic protein from SGE of An. sundaicus as the first step for the characterization of its immunomodulatory component. Mosquito salivary gland protein profile of An.sundaicus was determine by SDS-PAGE. Determination of salivary glands immunogenic proteins was conducted by Western Blotting with IgG from people living from endemic area as primary antibody. Out of 15 bands appeared in SDS PAGE ranging from 24 kD to 138 kD, only two protein bands with molecular weights of 68 and 37 kDa were the most immunogenic. Those immunogenic proteins were consistent recognized by pooled serum of people as well as by individual response. Keywords: malaria, saliva, vector, immunogenic protein, vaccine

Potential Use of Mosquito’s Salivary Components as Novel Target for The Development of Transmission Blocking Vaccine (TBV) KARTIKA SENJARINI
Microbiology Indonesia Vol. 7 No. 4 (2013): November 2013
Publisher : Indonesian Society for microbiology

Mosquito-borne diseases are rampant in most tropical regions of the world, especially rural, forested, and coastal areas such as Indonesia. Despite long-standing chemotherapeutic intercession and vector control programs, mosquito-borne diseases exact a heavy burden on human health in Indonesia. Two major public health problems transmitted by mosquito in Indonesia are malaria and dengue haemorrhagic fever (DHF), causing millions of clinical episodes occurring annually. Malaria is now recognized as a serious re-emerging threat to public health. DHF cases were first observed in 1968; since then, the incidence has been constantly increasing and the disease is now one of the principal causes of child lethality. It has been widely observed that saliva of mosquito that transmits the diseases contains several factors that could enhance pathogen infection. Therefore, it should be possible to control pathogen transmission by vaccinating the host against the molecule(s) in saliva that potentiate the infection. However, specific component as a potential target for TBV in mosquito vectors of malaria & dengue, i.e. Anopheles and Aedes aegypti, has not been identified so far. This paper wanted to elaborate the potential role of salivary component from mosquitoes, particularly from Indonesian vectors as molecular target for developing TBV against two major Mosquito borne-diseases in Indonesia i.e. malaria and DHF.

Isolation of Genes Encoding Arthropod Odorant Binding Proteins (OBP), D7 from Salivary Gland Vectors of Malaria: Anopheles sundaicus Nuryatmaja Gora Pawana; Kahar Muzakhar; Kartika Senjarini
Jurnal ILMU DASAR Vol 16 No 1 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v16i1.621

The isolation of Arthropod Odorant Binding, D7 protein, encoding genes from Anopheles sundaicus and An. maculatus mosquitos as the malaria vectors in Indonesia is necessary to recognize their characteristic. The isolated genes can be used to develop the Transmission Blocking Vaccine (TBV). This research aims to characterize the D7 protein encoding genes from An. sundaicus and An. maculatus through the synthesis of complementary DNA (cDNA) of D7 protein by using D7 protein primer that has been used for the other species of Anopheles. The mosquitos were taken from Dusun Parasputih, Bangsring, Wongsorejo, Banyuwangi, Jawa Timur. Isolation of the salivary gland was done by performing microdisection method and the isolation of the total RNA was done by performing High Pure RNA Isolation Kit (Roche-Germany). Synthesis of cDNA D7 encoding gene and its amplification were performed by using Maxime RT-PCR Premix Kit (iNtRon Biotechnology). The result of the total RNA and RT-PCR were run in agarose gel and visualized under the UV transiluminator. Based on the visualization, we found that the salivary gland total RNA of female An. sundaicus was 500-750 base pair (bp). The RT-PCR visualization showed a band sized below 100 bp and it was concluded not to be the size of the D7 protein encoding gene. An incompatibility of D7 primer from An. gambiae with cDNA template from An. sundaicus was suspected to be the reason of the gene isolation failure.Keywords: gene isolation, D7 protein, salivary gland, Anopheles sundaicus

Are Bacteria The Main Producers of Hydrolytic Enzymes in Aquatic Environment Kartika Senjarini; Ulf Karsten; Rhena Schumann
Jurnal ILMU DASAR Vol 9 No 1 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.046 KB)

Bacteria play an important role in the decomposition of organic matter, which is a key process in aquatic microbial food webs as well as its application in water bioremediation processes. DOM (Dissolved Organic Matter) is the major fraction of organic matter in most aquatic environments. Most of DOM is present as high molecular weight compounds that cannot be taken up by bacteria directly. Therefore, they must be hydrolysed enzymatically to be transported across microbial cell membranes. Molecular fluorosensors have been used as artificial substrates to study hydrolytic enzymes in situ. The present study wants to investigate the following hypothesis i.e. bacteria are the main producers of hydrolytic enzyme in aquatic systems. There were not any significant correlations between bacteria and the investigated hydrolases (esterase, peptidase, and β-glucosidase) in the meso- to eutrophic aquatic systems near Rostock city – North East Germany, although a wide range of bacterial abundances were covered. Enzyme saturations were not detected in all samples which may be explained by a high KM indicating a low affinity of enzymes (1) and or the contribution of many enzymes with different kinetics to the respective substrate degradation (2). There is also increasing evidence that bacteria may not be the sole or dominant source of esterases, peptidases and β-glucosidases in aquatic ecosystems. Invertebrate, fungi and other eukaryotes (diatoms, protozoa etc.) must be considered as possible and even important producers of hydrolytic enzymes. Several other factors may influence the correlations of bacteria to hydrolytic enzyme activities, i.e. variations in the species composition (1), a wide ranged variability of hydrolytic activities influenced directly by other substrates (low enzyme affinity), element availability (N and P), temperature and other abiotic factors (2), the existence and persistence of enzymes caused by other (passive) processes (e.g. cell lysis) (3).

Profile of Interleukin-4 and Interferon-γ of Balb/c Mice after Salivary Gland Extract of Anopheles sundaicus s.l. Vaccination and Infected by Plasmodium berghei-ANKA Ali Machrus; Adrial Adrial; Yunita Armiyanti; Hidayat Teguh Wiyono; Kartika Senjarini
Jurnal ILMU DASAR Vol 15 No 2 (2014)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Malaria infection is initiated when sporozoites are inoculated into a vertebrate host via the salivary glands of an Anopheles mosquito. During Anopheles bite, the salivary glands release components that include vasomodulator and immunomodulators. The salivary components of vectors have important role in transmission of pathogen. Therefore, if these components were injected repeatedly into a vertebrate host, it can stimulate host immune system and inhibit the transmission of the pathogen into the host. This could be observed the increasing level of IFN-γ and decreasing level of IL-4 in mice model of malaria after vaccination with salivary gland ekstract (SGE) from An sundaicus s.l. It has also been proven that this mechanisms was related with pathogen of malaria. This was supported by the reduction of parasitemie rate in those mice model after infection by P. Berghei. Keywords: An sundaicus s.l., IFN-γ, IL-4,immunomodulators, salivary gland , TBV

Deteksi Aktivitas Fibrinolitik Isolat Bakteri WU 021055* Asal Perairan Pantai Papuma Jember Menggunakan Zimografi Evi Umayah Ulfa; Esti Utarti; Izzay Afkarina; Sattya Arimurti; Kartika Senjarini
Global Medical & Health Communication (GMHC) Vol 5, No 2 (2017)
Publisher : Universitas Islam Bandung

Bakteri merupakan sumber penting berbagai enzim termasuk enzim fibrinolitik. Enzim ini diperlukan untuk mendegradasi bekuan darah pada orang yang mengalami penyakit trombosis. Isolat bakteri WU 021055* asal Pantai Papuma Jember terbukti menghasilkan enzim fibrinolitik ekstraseluler. Penelitian ini bertujuan mengetahui ukuran protein yang memiliki aktivitas fibrinolitik dan mengidentifikasi karakteristik morfologi isolat WU bakteri WU 021055*. Penelitian ini dilakukan di Laboratorium Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Jember pada April–Agustus 2014. Aktivitas fibrinolitik presipitat protein (PP) ditentukan menggunakan metode fibrin plate agar dan zimografi fibrin. Ekstrak protein kasar (EPK) dipanen pada jam ke-12 dan dipresipitasi menggunakan amonium sulfat 80%. Hasil uji aktivitas fibrinolitik menggunakan fibrin plate agar menunjukkan presipitat memiliki aktivitas fibrinolitik lebih besar dibanding dengan EPK. Dari hasil karakterisasi PP menggunakan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) diperoleh 11 pita protein dengan ukuran 12–41 kDa. Berdasar atas hasil zimografi fibrin, pita protein dengan berat molekul 24 kDa yang memberikan aktivitas fibrinolitik. Protein dengan ukuran 24 kDa ini mampu mendegradasi substrat fibrin. Simpulan, isolat bakteri WU 021055* mengandung berbagai protein ekstraseluler, memiliki bentuk koloni bulat berwarna putih dan termasuk bakteri gram prositif berbentuk batang.DETECTION OF FIBRINOLYTIC ACTIVITY OF WU 021055* BACTERIAL ISOLATE FROM PAPUMA BEACH COASTAL JEMBER USING ZYMOGRAPHYBacteria were important resources for various enzymes including fibrinolytic enzymes. This enzyme is capable of degrading fibrin clot in patient with thrombotic diseases. Bacterial isolate of WU 021055* from Papuma Beach Coastal Jember could secrete extracellular fibrinolytic enzymes. The objective of this reasearch was to determine the molecular weight of protein responsible for fibrinolytic activity and to identify morphologycal characterization of bacterial isolate of WU 021055*. This study was conducted at Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Universitas Jember in April–August 2014. Fibrinolytic activity of precipitate protein (PP) was determined by using fibrin plate agar and fibrin zymography. Crude protein extract (CPE) was harvested at 12 hours and precipitated by 80% ammonium sulphates. The result of fibrinolityc activity determination showed that fibrinolytic activity of PP was higher than CPE. Protein characterization of PP by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) obtained 11 different protein bands corresponds to value 12–42 kDa. Based on fibrin zymography, the 24 kDa protein might contribute to fibrinolytic activity due to degraded fibrin substrates. In conclusion, bacterial isolate of WU 021055* contained extracellular fibrin protein was white colony and gram positives bacilli able to degraded.

The Development Strategies on Batik Creative Industry as an Enthralling Sector for Cultural Tourism in Bondowoso Regency, East Java Syubbanul Wathon; Kartika Senjarini; Rike Oktarianti
Warta Pengabdian Vol 13 No 2 (2019): Warta Pengabdian
Publisher : LP2M Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/wrtp.v13i2.10237

Bondowoso Regency has great potential to evolve into the centers of the batik creative industry. But, there are still no batik products that show the uniqueness of Bondowoso Regency. Several community groups in Suling Wetan Village, Cermee Subdistrict, Bondowoso Regency, have been running a business as batik artisans since 2015. The community groups have had the basic skill to produce batik. Unfortunately, they still don't give any impact on the growth of the cultural tourism destinations in Bondowoso. The old batik designs do not attract buyers, has limited color combinations, and only rely on synthetic batik dyes. Through the partnership service program that have been implemented, some of the Batik community business can develop their products. This partnership service program is done by several activities, such as socialization; workshops on batik design and the use of natural dyes; training on natural materials and batik coloring; procurement of gawangan machines, pendulum and accessories; training and mentoring techniques for making interesting batik motifs and coloring using natural dyes; and the dialogue to develop the creative batik industry. The results of this program are developing not only the Suling Wetan Village but also make the Batik community business in Cermee Subdistrict, independently growth as a creative batik industry. This improvement is also captivating the cultural tourism in Bondowoso Regency.

MORPHOLOGICAL CHARACTERISTIC DIFFERENCE BETWEEN MOSQUITOES VECTOR FOR MALARIA AND DENGUE FEVER Syubbanul - Wathon; Kartika Senjarini; Rike Oktarianti; Ratis Nour Sholichah; Ahmad Tosin
BIOEDUKASI Vol 18 No 2 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i2.18890

Malaria and Dengue Haemorrhagic Fever (DHF) are two major tropical diseases in East Java. The pathogens of these diseases are transmitted to human hosts via haematophagy by vector mosquitoes. Anopheles spp. specifically transmits Plasmodium parasites which cause Malaria, while Aedes spp. transmits Dengue viruses that cause DHF. Bangsring, Banyuwangi is one of the endemic areas of Malaria, while Jember is one of endemic areas of DHF. Two species of Malaria vectors i.e Anopheles vagus and Anopheles sundaicus have been found from several samplings which were conducted in Bangsring region. This mosquitoes have been previously identified as a major vector for malaria. Aedes aegypti and Aedes albopictus, which were major vectors for DHF, have been easily found in Jember during our sampling for any season of the year. This research wanted to differentiate the morphological characteristic of those vectors for malaria and Dengue. Adult mosquitoes can be distinguished from one another by characterizing their morphological features. Palpi, wings, and legs characteristics are commonly used as determination keys in Anopheles sp., while thoraxes and legs characteristics are used as identification keys in Aedes sp. Morphological identification is considered as the basic necessity in understanding and determining bionomic of mosquito vector. This is very important in developing effective and efficient mosquito vector control strategies, which is also an important step to prevent the death risks associated with Malaria and DHF cases.

THE IMMUNOGLOBULIN (IgG) ANALYSIS OF IMPLANTATION MICE (Mus musculus L.) POST OVARIECTOMY AFTER TREATMENT OF BLACK SOYBEAN FLOUR EXTRACT (Glycine soja) Yenny Febriana Ramadhan Abdi; Mahriani Mahriani; Kartika Senjarini; Rike Oktarianti
BIOEDUKASI Vol 19 No 1 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i1.21099

Black soybean is one type of plant that has estrogenic activity, it can act as a source of natural exogenous estrogen. Decreased estrogen hormone in the body due to ovariectomy and menopause, it affect on immune response (IgG). The purpose of this study was to determine the effect of black soybean extract on immune response (IgG) of implantation mice post ovariectomy. Twenty eight mice were grouped into 4 groups, group 1 (negative control), group 2 (positive control with the administration estradiol concentration 50 ppm), group 3 and 4 were treatment group with the administration of black soybean flour extract doses of 0.31 g/ml and 0.63 g/ml. The data were analyzed using One Way Anova and Duncan Multiple Range Test (DMRT) (p<0,05). The treatment with administration of black soybean flour extract for 10 days in implantation mice post ovariectomy showed that a dose of 0.31 g/ml and dose of 0.63 g/ml had an effect on increasing significantly the immune response (IgG), i.e 0,12987and 0,12996 respectively.

Co-Authors Adrial Adrial Agustin, Dita Paramytha Ahmad Tosin Ainiyah, Durotun Ajeng Maharani Sri Pananjung Ajeng Maharani Sri Pananjung, Ajeng Maharani Ali Machrus Anak Agung Gede Sugianthara Anam, Khairul Ardyah, Naura Paramitha Cindy Ari Satia Nugraha Arif Setiawan Azkiyah, Siti Fat'hiyatul Bagus Setiawan Berlian Permata Dewi Erlambang Budayatin, Budayatin Cizein Tri Cahyanti Devi Astikaningrum Devi Astikaningrum Dewi Masruroh Didik SULISTYANTO DINA FITRIYAH Dita Paramytha Agustin Esti Utarti Evi Umayah Ulfa Febriyantiningsih, Dwi Esti Fitria Mutiah Fitria Muti’ah Hidayat Teguh Wiyono Husnul Hotimah, Husnul Ihsanul Azmi Intan Fitri Indrasari Izzay Afkarina Kahar Muzakhar Khasanah, Rochmatul Nuryu Labes, Antje Lailly Nur Uswatul Hasanah Lelono, Asmoro Loeki Enggar Fitri Mahriani Mahriani MAsruroh, Binti Maulana Jauharil Habib Miatin Alvin Septianasari Mochtar Gunawan Wibisono Moh Mirza Nuryady Moh Mirza Nuryady, Moh Mirza Muhammad Khalid Abdullah Mutiah, Fitria Nadya Rismana Fitriani Naura Paramitha Cindy Ardyah Naura Paramitha Cindy Ardyah Nuril Azizah Nuryatmaja Gora Pawana Purwatiningsih Purwatiningsih Ratis Nour Sholichah Rehmann, Holger Renam Putra Arifianto Rhena Schumann Riana Agatha Listiani Rike Oktarianti Sattya Arimurti Septiawan, Mahful Silvya Fitri Nur Azizah Sri Mumpuni Wahyu Widajati, Sri Mumpuni Wahyu Sugeng Setyo Utomo Sugeng Setyo Utomo, Sugeng Setyo Sutoyo Sutoyo Syubbanul Wathon, Syubbanul Syubbanul Waton Syubhanul Wathon Teguh Wahju Sardjono Teguh Wahju Sardjono Tri Yudani MR Ulf Karsten Utami, Diah Ayu Wathon, Syubanul Widya Cahyadi Yasir Mubarok Yenny Febriana Ramadhan Abdi Yunita Armiyanti

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