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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Indonesian Journal of Electronics and Instrumentation Systems Jurnal Ilmu Dasar Microbiology Indonesia BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Journal of Agromedicine and Medical Sciences Global Medical and Health Communication UNEJ e-Proceeding Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI Warta Pengabdian Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Jurnal Riset Biologi dan Aplikasinya Life Science and Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi

Kartika Senjarini

Biology Department, Faculty Of Mathematic And Natural Sciences, University Of Jember

Author-ID : 375743

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Dentistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Nursing Physics Public Health Veterinary

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PURIFICATION OF 31 AND 67 kDa PROTEIN FRACTION FROM SALIVARY GLAND OF Aedes Albopictus (SKUSE) (DIPTERA: CULLICIDAE) Syubbanul Wathon; Rike Oktarianti; Nuril Azizah; Yasir Mubarok; Riana Agatha Listiani; Kartika Senjarini
BIOEDUKASI Vol 19 No 1 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i1.18892

Aedes albopictus mosquito is a potential vector for Dengue Haemorrhagic Fever (DHF) which transmits Dengue virus during blood feeding. The success of the blood feeding process is aided by the biological activity of proteins in the salivary glands of Aedes albopictus. There are 30 types of proteins from the salivary glands of Aedes albopictus which are carried along blood feeding process. Proteins in the salivary glands act as vasodilator and immunomodulator. Previous studies have identified two immunogenic proteins from the salivary glands of Aedes albopictus with molecular weight of 31 and 67 kDa. Further research on the biological function of these proteins requires its purified protein to better specify the target to developing a dengue vaccine. The objective of this study was to obtain 31 and 67 kDa purified proteins by implementation of electroelution and dialysis purification. The 31 and 67 kDa protein was successfully purified by this method. This has been confirmed by a single band visualization after SDS-PAGE analysis

PLATELET AGGREGATION IN VITRO ANALYSIS OF 67 kDa IMMUNOGENIC PROTEIN FRACTION FROM Aedes albopictus SALIVARY GLAND (SKUSE) (DIPTERA: CULICIDAE) Rike Oktarianti; Syubbanul - Wathon; Intan Fitri Indrasari; Nadya Rismana Fitriani; Kartika Senjarini
BIOEDUKASI Vol 18 No 2 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i2.18891

Cardiovascular disease is caused by impaired heart and blood vessel function. Coronary heart disease includes acute coronary syndrome due to narrowing of the coronary arteries. Aspirin is an anti-platelet drug which is commonly used for primary and secondary prevention of coronary heart disease. The effectiveness of aspirin has limitations because 10-20% of patients who use aspirin continue to experience vascular blockage. The haematophagus arthropods salivary glands contain apyrase which can inhibit platelet aggregation and thus a potential candidate for anti-platelet drug discovery. Our studies from salivary gland protein extract of Aedes albopictus found a 67 kDa protein fraction which has a similar molecular weight range from the previously identified apyrase of other mosquitoes vectors. The purpose of this study was to determine the apyrase potential capacity of 67 kDa protein fraction from salivary gland of Ae. albopictus. The present study employed inhibition percentage of platelet aggregation method in determining its apyrase activity. Aspirin was used as a positive control with 2 different concentrations of 0.1 mg/mL and 2 mg/mL. PBS 1mM pH 7.4 was used as a negative control treatment, while negative control without treatment only involved the addition of PRP and ADP. The inhibition percentage activity from the 67 kDa sample ranged from 3-37% whereas the total protein extract comprised of only 1%. The positive control of aspirin was 0.1 mg/mL and 2 mg/mL, resulting in inhibition percentage of 5% and 17%, respectively. The data showed that the inhibition percentage of platelet aggregation from protein 67 kDa is generally higher than those of total salivary gland protein extract as well as positive control. This indicated that the 67 kDa protein has a potential apyrase activity.

KARAKTERISASI BERBASIS MARKA MOLEKULER ITS2 TERHADAP SUB-SPESIES KOMPLEKS Anopheles vagus vagus DAN Anopheles vagus limosus Kartika Senjarini; Lailly Nur Uswatul Hasanah; Miatin Alvin Septianasari; Muhammad Khalid Abdullah; Rike Oktarianti; Syubbanul Wathon
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

The presence of intraspecies variations of An. vagus later categorized as the subspecies of An. vagus vagus and An. vagus limosus, could be an obstacle to the identification process, which is an important step for malaria vector’s competence characterization. Based on morphological identification, those subspecies could be distinguished by the presences of pale scales in prehumeral and pale bands in proboscis. The objective of this research was to compare subspecies complexes of An. vagus morphologically and molecularly using Internal Transcribed Spacer 2 (ITS2). Anopheles samples were collected from Bangsring, Banyuwangi. Their phylogenetic tree was constructed by using NJ method based on their ITS2 sequences. BLAST result showed that An. vagus vagus and An. vagus limosus were similar to An. vagus FJ654649.1 from East Java Indonesia and East Timor based on its 99% homology and their molecular distance. The Neighbour Joining (NJ) tree grouped those subspecies in one clade with a boostrap value of 82%. This subspeciation might be due to the different rates of evolution. ITS2 sequences of An. vagus vagus and An. vagus limosus were submitted to GenBank with the accession number of MW314227.1 and MW319822.1, respectively. Kemunculan variasi intraspesies An. vagus yang kemudian dikategorikan sebagai subspesies An. vagus vagus dan An. vagus limosus menjadi kendala dalam proses identifikasi yang merupakan langkah penting dalam menentukan kompetensi vektor malaria. Berdasarkan karakter morfologi, subspesies tersebut dibedakan dengan adanya sisik pucat pada bagian prehumeral dan pita pucat pada probosis. Penelitian ini bertujuan untuk membandingkan subspesies An. vagus secara morfologis dan molekuler menggunakan Internal Transcribed Spacer 2 (ITS2). Nyamuk Anopheles didapatkan dari Bangsring, Banyuwangi. Konstruksi pohon filogeni dilakukan berdasarkan sekuen ITS2 yang dianalisis menggunakan metode NJ. Hasil BLAST menunjukkan, ITS2 An. vagus vagus dan An. vagus limosus memiliki tingkat homologi 99% dan jarak evolusi molekuler terendah dengan An. vagus FJ654649.1 dari Jawa Timur Indonesia dan Timor Timur. Pohon NJ mengelompokkan subspesies tersebut dalam satu klade dengan nilai boostrap 82%. Hal ini dapat terjadi karena perbedaan kecepatan evolusi yang memungkinkan terjadinya subspesiasi. Urutan basa ITS2 dari An. vagus vagus dan An. vagus limosus telah didaftarkan ke GenBank dengan nomor aksesi MW314227.1 dan MW319822.1.

USING sma-its2 PRIMER FOR ITS2 (Internal Transcribed Spacer-2)-BASED MOLECULAR CHARACTERIZATION OF Anopheles minimus FROM KULONPROGO, YOGYAKARTA - INDONESIA Syubbanul Wathon; Berlian Permata Dewi Erlambang; Naura Paramitha Cindy Ardyah; Devi Astikaningrum; Rike Oktarianti; Kartika Senjarini
BIOEDUKASI Vol 20 No 1 (2022)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v20i1.31911

Malaria is a public health threat caused by the Plasmodium infection transmitted by the Anopheles mosquito. Malaria vector control is highly dependent on the ability to determine mosquito species' vectorial and bionomic capacity. Species identification based on morphological characteristics as well as DNA-barcoding approaches is a very important step in determining vectorial capacity. Our research group has redesigned a new ITS2 primer, namely, sma-its2, which is specifically able to identify the Anopheles (An.) mosquito vector but cannot identify other mosquito vectors. This study wanted to test this primer's specificity further for identification of other Anopheles mosquitoes. We used An. minimus collected from Kulonprogo, Yogyakarta – Indonesia. The methods used in this research are as follows: landing collection, morphological identification, isolation of genomic DNA, PCR (Polymerase Chain Reaction), PCR product purification, sequencing, and data analysis. An. vagus from Bangsring, Banyuwangi – Indonesia, which had previously been identified using the same primary, was used as a positive control. The results of the morphological analysis showed that both species were in accordance with the vector identification key used in this study. The molecular analysis showed that the sma-its2 primer could amplify the ITS2 sequence of An. vagus and An. minimus, producing 650 – 700 bp. However, further analysis of the ITS2 sequences of both species, resulted in the same species, namely An. vagus, with a different accession number in GenBank. This showed that the sma-its2 primar can be used to identify An. vagus but cannot be used to identify An. minimus. Analysis of the primer position in the ITS2 sequences showed the presence of 3 nucleotides in the forward sma-its2 primer that was not recognized by the An. minimus sequences and thus, hinder the successful identification of these species.

In silico study to predicting Apyrase-ADP binding affinity from salivary gland of Aedes aegypti in inhibition of platelet aggregation by molecular docking Rike Oktarianti; Silvya Fitri Nur Azizah; Ari Satia Nugraha; Syubbanul Wathon; Kartika Senjarini
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 2 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i2.33227

ANOPHELES MICROBIOTA IN MALARIA VECTOR AND THE EFFECT ON PARASITE TRANSMISSION Dita Paramytha Agustin; Berlian Permata Dewi Erlambang; Cizein Tri Cahyanti; Syubbanul Waton; Rike Oktarianti; Kartika Senjarini
BIOEDUKASI Vol 19 No 2 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i2.25165

Malaria is a vector-transmitted disease with a high number of cases. Plasmodium parasites are transmitted from the body of the Anopheles mosquito to the host through several mosquito organs, including the salivary glands and the midgut. The salivary glands and the midgut, apart from being a breeding ground for parasites, are known from several studies that various types of microbiota inhabit these two organs. The group of bacteria is the most widely known to be associated with Anopheles mosquitoes. The bacteria found were dominated by Gram-negative bacteria, with Pseudomonas being the most common, followed by Aeromonas, Asaia, Comamonas, Elizabethkingia, Enterobacter, Klebsiella, Pantoea, and Serratia. The group of Gram-positive bacteria was represented by the genera Bacillus, Streptococcus, Lactobacillus, and Staphylococcus. Each species of Anopheles has a different composition of associated bacteria. Anopheles-associated bacteria currently receive much attention because of their role in fighting Plasmodium infection. The ability of malaria vector-associated bacteria to inhibit and fight Plasmodium infection is divided into three mechanisms. Bacteria can triger an immune response through the Immune-deficiency (IMD) pathway, which causes active anti-Plasmodium and the production of antimicrobial peptides, increasing ROS synthesis and microbiota, which trigger hemocyte differentiation to protect against Plasmodium. This function related to inhibiting Plasmodium development needs further research so that later it can become an option for vector-based malaria control without damaging the sustainability of the environment.

In silico exploration of the potential barcode DNA in Anopheles sp., a malarian vector from Indonesia Syubbanul Wathon; Devi Astikaningrum; Naura Paramitha Cindy Ardyah; Rike Oktarianti; Kartika Senjarini
Jurnal Biolokus Vol 6, No 1 (2023): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v6i1.1749

Malaria is an infectious disease caused by Plasmodium and transmitted to humans through the Anopheles mosquito vector. The large diversity of Anopheles in Indonesia, which consists of similar complex species, makes it difficult to identify species based on morphological characteristics. Therefore, it is necessary to have another approach to identifying species based on molecular characteristics using DNA molecular markers. This approach becomes important as a basis for vector-based malaria control efforts. This research aims to explore potential DNA barcodes based on in silico studies as a reference for DNA barcoding analysis of Anopheles species in Indonesia. The data sequences of CO1 and ITS2 from Anopheles sp. were collected on the NCBI database. The sequence alignment was performed using CLUSTALX2. The construction of a phylogenetic tree was carried out using MEGA XI. Data analysis was performed by observing the construction of phylogenetic trees, bootstrap scores, and genetic distance. The data sequence in NCBI shows that 10 species of the genus Anopheles as malaria vectors have been studied in Indonesia. Sequence alignment of the 10 species based on CO1 and ITS2 markers showed that ITS2 markers had higher genetic variation than CO1. Therefore, the ITS2 sequence has the potential to be a DNA barcode for the molecular identification of Anopheles.

Relative Frequency (Composition) of Anopheles sp. Mosquitoes as Malaria Vectors in Kulon Progo District Special Region of Yogyakarta Wathon, Syubbanul; Senjarini, Kartika; Masruroh, Binti; Oktarianti, Rike; Rehmann, Holger
Jurnal Riset Biologi dan Aplikasinya Vol. 6 No. 1 (2024)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v6n1.p20-33

Malaria is caused by Plasmodium infection transmitted Anopheles sp. One of the obstacles in malaria control is the variation of Anopheles sp. Species which have various characteristics and behaviour, so it’s very important to understand the species of Anopheles sp. with aim to develop the disease control program. This study was conducted in Kulon Progo Regency, Yogyakarta, one of the malaria endemic areas in Indonesia. Data collection of Anopheles sp. species diversity was carried out through landing collection inside and outside people’s homes at 18.00 - 06.00 WIB. Observations of abiotics factors in the habitat of Anopheles sp. larvae was carried out around the landing collection location. The result of research conducted in the Samigaluh, Kalibawang, and Pengasih Sub-Districts showed that the most common species found was Anopeheles vagus. The dominance of Anopheles vagus occurred in the Kalibawang and Pengasih dictricts, while in the Samigaluh Sub-Districts it became the second most among other species. Measurement of abiotic factors in the habitat of Anopheles sp. larvae through the parameters of temperature, air humidity, pH DO of water shows that the location where the larvae were found can support larval breeding and growth. That can be a factor in the large population of Anopheles sp., especially Anopheles vagus, which is a known vector of malarias based on research and its vectorial capacity.

Identification of Immmunogenic Salivary Proteins of Anopheles vagus based on Mass Spectrometry Analysis Febriyantiningsih, Dwi Esti; Senjarini, Kartika; Oktarianti, Rike
Jurnal ILMU DASAR Vol 18 No 2 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Malaria has been prevalent for a long time in tropical developing regions causing great morbidity and mortality. Among the malaria vectors, Anopheles vagus has been known as secondary malaria vector in East Java. Salivary glands of mosquitoes perform various functions for survival of the vectors and also conducive for blood feeding, harbouring of malaria parasites, and eventual parasite transmission. The salivary gland proteomes of An. vagus have not been carried out yet. The aim of our study was to identify and characterize the immunogenic proteins of salivary glands proteins of An. vagus. A proteomic approach combining one-dimensional electrophoresis (1DE) followed by western blot analysis using human sera from healthy people living in an endemic area (Kendal); liquid chromatography mass spectrometry (LC-MS/MS) and bioinformatic analysis was adopted to provide the first direct insight into identification and characterization of salivary proteins of An. vagus. Identification of immunogenic proteins using western blot analysis has revealed three immunogenic bands which had molecular weights of 69, 75 and 232 kDa. Among those proteins analysed by LC-MS/MS, there were alpha,1-4 glucan phosphorylase, putative myosin class I heavy chain which have the highest number of total spectrum count peptide. Other proteins like vitellogenin and heat shock protein 82 (Hsp82) were also identified. The majority of proteins were scrutinized marked for their role in metabolism, cytoskeleton protein and stress response. Keywords: Anopheles vagus, salivary gland, immunogenic, proteomics

Morphological Description of Drosophila melanogaster Wild Type (Diptera:Drosophilidae), Sepia and Plum Strain Hotimah, Husnul; Purwatiningsih, Purwatiningsih; Senjarini, Kartika
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Drosophila melanogaster is one of the insects which have a very important role in the development of the genetic science. Drosophila melanogaster have many mutation, recently there are many mutant such as sepia and plum strain. Morgan et al have found 85 strain mutan of Drosophila melanogaster. The purpose of these research was to characterize of morphological from the head, thorax, and abdomen. The result show that the morphological of Drosophilla melanogaster wild type, sepia and plum mutant have many similarities. The difference of them are the eyes color, Drosophila melanogaster wild type has red eye, sepia strain has a dark brown eye and plum has a dark purple eye.

Co-Authors Adrial Adrial Agustin, Dita Paramytha Ahmad Tosin Ainiyah, Durotun Ajeng Maharani Sri Pananjung Ajeng Maharani Sri Pananjung, Ajeng Maharani Ali Machrus Anak Agung Gede Sugianthara Anam, Khairul Ardyah, Naura Paramitha Cindy Ari Satia Nugraha Arif Setiawan Azkiyah, Siti Fat'hiyatul Bagus Setiawan Berlian Permata Dewi Erlambang Budayatin, Budayatin Cizein Tri Cahyanti Devi Astikaningrum Devi Astikaningrum Dewi Masruroh Didik SULISTYANTO DINA FITRIYAH Dita Paramytha Agustin Esti Utarti Evi Umayah Ulfa Febriyantiningsih, Dwi Esti Fifteen Aprila Fajrin Finasrullah, Aufar Fitria Mutiah Fitria Muti’ah Hidayat Teguh Wiyono Husnul Hotimah, Husnul Iflaha, Adriana Ihsanul Azmi Intan Fitri Indrasari Izzay Afkarina Kahar Muzakhar Khasanah, Rochmatul Nuryu Labes, Antje Laili, Alvina Nur Lailly Nur Uswatul Hasanah Lelono, Asmoro Loeki Enggar Fitri Mahriani Mahriani MAsruroh, Binti Maulana Jauharil Habib Miatin Alvin Septianasari Mochtar Gunawan Wibisono Moh Mirza Nuryady Moh Mirza Nuryady, Moh Mirza Muhammad Khalid Abdullah Muhammadiy, Noer Sidqi Mutiah, Fitria My, Truong Nhat Nadya Rismana Fitriani Naura Paramitha Cindy Ardyah Naura Paramitha Cindy Ardyah Nuril Azizah Nuryatmaja Gora Pawana Purwatiningsih Purwatiningsih Rahmawati, Itsna Ratis Nour Sholichah Rehmann, Holger Renam Putra Arifianto Rhena Schumann Riana Agatha Listiani Rike Oktarianti Saraswati, Dyah Rahmadhani Sattya Arimurti Septiawan, Mahful Silvya Fitri Nur Azizah Song, Le Huu Sri Mumpuni Wahyu Widajati, Sri Mumpuni Wahyu Sugeng Setyo Utomo Sugeng Setyo Utomo, Sugeng Setyo Sutoyo Sutoyo Syubbanul Wathon, Syubbanul Syubbanul Waton Syubhanul Wathon Teguh Wahju Sardjono Teguh Wahju Sardjono Tri Yudani MR Ulf Karsten Utami, Diah Ayu Velavan, Thirumalaisamy P. Wathon, Syubanul Wathon, Syubbanul - Widya Cahyadi Widyadana, Kaysha Rahmadita Yasir Mubarok Yenny Febriana Ramadhan Abdi Yunita Armiyanti Zahniar, Zahniar

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