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Journal : Jurnal Agroekoteknologi

ISOLASI BERBAGAI MIKROBA PADA RHIZOSFIR TANAMAN JAGUNG ., Nurmayulis; Setiawati, Mieke Rochimi; Utama, Putra; ., Susiyanti
Jurnal Agroekoteknologi Vol 1, No 2 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB) | DOI: 10.33512/j.agrtek.v1i2.596

Abstract

ABSTRACT Biodiversity of potential  soil microbes has been regarded as biological fertilizer of Zea mays in marginal land. A few microbe increased the rate of growth  of plant throught absorbtion slow released N and P in soil. The isolation, identification and population of soil microbes from rizosphere at Walantaka (Banten province) had been done in the Soil Biology Laboratories and the greenhouse, Pajajaran University- Bandung, during Mei to Agustus 2009. The soil was collected randomly from sites in Walantaka. To increase N and P using trapping and selection medium from from rizosphere at Walantaka (centre of Zea mays production), province of Banten.  The Exploration of potential microbe Isolates of Azotobacter, Azospirillum, microbes dissolver phosphate (MPF) dan Mycorrhyzae microbes were identified and were founded in soil samples. The result showed, on okon selection medium  can be found 4 Azospirillum, on Ashby medium  lived 4 Azotobacter,  and Pikovskaya medium got 2 MPF and Mycorrhyzae microbes.Key words: marginal land, Azospirillum, Azotobacter, MPF, Mycorrhyzae, Zea mays
PENGARUH NaCl DAN AMELIORAN ORGANIK Azolla pinnata TERHADAP N-TOTAL, pH TANAH, SERTA BOBOT KERING TANAMAN PADI (Oryza sativa L.) PADA INCEPTISOLS JATINANGOR Khoiriyyah, Luluatul; Setiawati, Mieke Rochimi; Suriadikusumah, Abraham
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB)

Abstract

The high content of NaCl in soil may lead to nutrient imbalance and inhibit the Nitrogen uptake by plants. Azolla pinnata can be used as an organic ameliorant to help plant roots in absorbing nutrients and enhancing the number of available N for plants. The aims of this research were to ascertain whether Azolla pinnata as organic ameliorant gave the significant difference on the enhancement of N content of soil, pH of soil, and dry weight of rice plant on various salinity level of soil and also to find out if Azolla pinnata can suppress salinity stress at certain salinity. The experiment was conducted at the Greenhouse of Faculty of Agriculture Universitas Padjadjaran, Jatinangor and also at the Laboratory of Soil Fertility and Plant Nutrion, Departement of Soil Science and Land Resources on November 2016 until March 2017. The experimental design used was randomized block design with 8 treatments and 4 replications. The treatments consist of without the organic ameliorant Azolla pinnata, and with the application of Azolla pinnata on salinity level 0, 2, 4, 6 mmhos cm-1. The results showed that the application of NaCl and Azolla pinnata gave the significant difference on soil pH, and dry weight of plant. The application of Azolla pinnata is not able yet to suppress the rice symptom of salinity stress at 2, 4, and 6 mmhos cm-1 on the enhancement of dry weight of plant. The application of Azolla at salinity 6 mmhos cm-1 was equivalent with salinity 2 mmhos cm-1 in producing dry weight of plant.
APLIKASI PUPUK HAYATI BAKTERI ENDOFITIK TERHADAP POPULASI BAKTERI ENDOFITIK, SERAPAN N DAN BOBOT KERING TANAMAN PADI (Oryza Sativa L.) PADA TANAH SALIN Utami, Deswita; Setiawati, Mieke Rochimi
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB)

Abstract

Productivity of rice crops (Oryza sativa) which grown in saline soils can improve due to application endophytic bacteria biofertilizer. Endophytic bacteria lives in plant tissues have an able to fix nitrogen from the air that for supply N to plant tissue more optimal. The research aims was to find the effects of endophytic bacteria biofertilizer on enhance bacterial populations, nitrogen uptake, and dry weight of rice grown in saline soil. This research was conducted from March 2016 to February 2017 in Soil Biology Laboratory and in Laboratory of Soil Chemistry and Fertility, Soil Science Department and Land Resources, Faculty of Agriculture, Universitas Padjadjaran and Experiment Field of Ciparanje, Faculty of Agriculture, Universitas Padjadjaran with 752 meters above the sea level. This research used randomized block design experiment which consist of eight combinations: A (salinity of 0 dS m-1), B (salinity of 0 dS m-1 + biofertilizer), C (salinity of 2 dS m-1), D (salinity of 2 dS m-1 + biofertilizer), E (salinity of 4 dS m-1), F (salinity of 4 dS m-1 + biofertilizer), G (salinity of 6 dS m-1), H (salinity of 6 dS m-1 + biofertilizer). The result showed that at salinity 2 dS m-1 rice plant still survive growing on salinity stress. Salinity treatment of 2 dS m-1 + endophytic biofertilizer have enhance affect to bacterial population, N uptake and dry weight of rice compared to salinity of 4 and 6 dS m-1
PENGARUH PUPUK ORGANIK (Azolla pinnata) TERHADAP C-ORGANIK TANAH, SERAPAN N DAN BOBOT KERING TANAMAN PADI (Oryza sativa L.) PADA TANAH DENGAN TINGKAT SALINITAS TINGGI Arafah, Mayang Sunduz; Setiawati, Mieke Rochimi; Nurbaity, Anne
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB)

Abstract

Utilizing organic fertilizer Azolla pinnata is needed especially for rice commodity which planted in salin soil in coast area for increasing the efficiency of inorganic fertilizer by adding organic matters. This research aimed to get the best combination of utilizing organic fertilizer Azolla pinnata to increase total organic carbon, nitrogen uptake, and dry weight of rice in saline soil. This research used Randomized Complete Block Design experiment which consisted of eight combinations of treatment. Those are treatment of salinity 0, 2, 4 and 6 mmhos cm-1 + without utilizing organic fertilizer Azolla pinnata and treatment of salinity 0, 2, 4, and 6 mmhos cm-1 + utilizing organic fertilizer Azolla pinnata. The result showed that utilizing organic fertilizer Azolla pinnata can reduce the negative effect from salinity which proven by increased N uptake of rice in treatment of salinity 6 mmhos cm-1.
PENGARUH BAKTERI ENDOFITIK DAN Azolla pinnata TERHADAP POPULASI BAKTERI ENDOFITIK, KANDUNGAN N, DAN BOBOT KERING P PADI (Oryza sativa L.) PADA TANAH BERSALINITAS Melani, Melani; Saribun, Daud Siliwangi; Setiawati, Mieke Rochimi
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB)

Abstract

Nitrogen deficiency is the main problem of rice growth in salt stress condition. Endophytic bacteria has an ability to enhance the number of available N in plant tissue by fixing N from the atmosphere, meanwhile Azolla pinnata has high content of N that can increase the available N in soil. The aim of this research was to know the limit of salinity stress tolerance in rice with application of endophytic bacteria and A. pinnata on enhancing the population of endophytic bacteria, N content, and dry weight of rice on soil with salinity. The research was conducted on March 2016 until March 2017 at the Green House of Ciparanje Universitas Padjadjaran. Samples were analized at the Laboratory of Soil Biology and Laboratory of Soil Fertility and Plant Nutrion, Faculty of Agriculture, Universitas Padjadjaran. The design experiment used was single factor randomized block of 8 treatments and 4 repetitions. The treatments consist of with and without the application of endophytic bacteria and A. pinnata on soil with salinity of 0, 2, 4, and 6 mmhos cm-1. The results showed that the application of endophytic bacteria and A. pinnata gave the significant difference on the population of endophytic bacteria, N content, and dry weight of rice up to 4 mmhos cm-1.
APLIKASI PUPUK HAYATI BAKTERI ENDOFITIK TERHADAP POPULASI BAKTERI ENDOFITIK, SERAPAN N DAN BOBOT KERING TANAMAN PADI (Oryza Sativa L.) PADA TANAH SALIN Utami, Deswita; Setiawati, Mieke Rochimi
Jurnal Agroekoteknologi Vol 9, No 2 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v9i2.5106

Abstract

Productivity of rice crops (Oryza sativa) which grown in saline soils can improve due to application endophytic bacteria biofertilizer. Endophytic bacteria lives in plant tissues have an able to fix nitrogen from the air that for supply N to plant tissue more optimal. The research was aimed to know the effects of endophytic bacteria biofertilizer on enhance bacterial populations, nitrogen uptake, and dry weight of rice grown in saline soil. This research was conducted from March 2016 until February 2017 in Soil Biology Laboratory and in Laboratory of Soil Chemistry and Fertility, Soil Science Department and Land Resources, Faculty of Agriculture, Universitas Padjadjaran and Experiment Field of Ciparanje, Faculty of Agriculture, Universitas Padjadjaran with 752 meters above the sea level. This research used Randomized Completely Block Design which consisted of eight combinations: A (salinity of 0 dS m-1), B (salinity of 0 dS m-1 + biofertilizer), C (salinity of 2 dS m-1), D (salinity of 2 dS m-1 + biofertilizer), E (salinity of 4 dS m-1), F (salinity of 4 dS m-1 + biofertilizer), G (salinity of 6 dS m-1), H (salinity of 6 dS m-1 + biofertilizer). The result showed that at salinity 2 dS m-1 rice plant still survive growing on salinity stress. Salinity treatment of 2 dS m-1 + endophytic biofertilizer have enhance affect to bacterial population, N uptake and dry weight of rice compared to salinity of 4 and 6 dS m-1.Keywords: Endophytic bacteria, Rice (Oryza sativa L.), Saline soils
PENGARUH NaCl DAN AMELIORAN ORGANIK Azolla pinnata TERHADAP N-TOTAL, pH TANAH, SERTA BOBOT KERING TANAMAN PADI (Oryza sativa L.) PADA INCEPTISOLS JATINANGOR Khoiriyyah, Luluatul; Setiawati, Mieke Rochimi; Suriadikusumah, Abraham
Jurnal Agroekoteknologi Vol 9, No 2 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v9i2.5109

Abstract

The high content of NaCl in soil may lead to nutrient imbalance and inhibit the Nitrogen uptake by plants. Azolla pinnata can be used as an organic ameliorant to help plant roots in absorbing nutrients and enhancing the number of available N for plants. The aim of this research was to ascertain whether Azolla pinnata as organic ameliorant gave the significant difference on the enhancement of N content of soil, pH of soil, and dry weight of rice plant on various salinity level of soil and also to find out if Azolla pinnata could suppress salinity stress at certain salinity. The experiment was conducted at the Greenhouse of Faculty of Agriculture Universitas Padjadjaran, Jatinangor and also at the Laboratory of Soil Fertility and Plant Nutrion, Departement of Soil Science and Land Resources on November 2016 until March 2017. The experimental design used was Randomized Completely Block Design with 8 treatments and 4 replications. The treatment was consisted of without the organic ameliorant Azolla pinnata, and with the application of Azolla pinnata on salinity level 0, 2, 4, 6 mmhos cm-1. The results showed that the application of NaCl and Azolla pinnata gave the significant difference on soil pH, and dry weight of plant. The application of Azolla pinnata is not able yet to suppress the rice symptom of salinity stress at 2, 4, and 6 mmhos cm-1 on the enhancement of dry weight of plant. The application of Azolla at salinity 6 mmhos cm-1 was equivalent with salinity 2 mmhos cm-1 in producing dry weight of plant.Keywords: NaCl, Organic ameliorant Azolla pinnata, Rice, Salinity
RESPON TANAMAN CABAI MERAH (Capsicum annum L.) YANG DIAPLIKASIKAN BAKTERI ENDOFITIK DIAZOTROF DAN PUPUK N TERHADAP POPULASI ENDOFITIK DIAZOTROF, KONSENTRASI N, DAN BOBOT KERING TANAMAN PADA INCEPTISOLS JATINANGOR Mieke Rochimi Setiawati; Pujawati Suryatmana; Emma Trinurani Sofyan
Jurnal Agroekoteknologi Vol 10, No 2 (2018)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v10i2.5819

Abstract

The aim of the research was to determine the effect of nitrogen-fixing endophytic bacteria and N fertilizer on population of nitrogen-fixing (diazotroph) endophytic bacteria, N concentration and dry matter of red chili plant (Capsicum annuum L.) on Inceptisols from Jatinangor. The experiment conducted design was Randomized Block Design in factorial pattern consisted of two factor and three replication. The first factor was diazotroph endophytic bacteriaconsisted two levels: without used diazotroph endophytic bacteria, and used diazotroph endophytic bacteria. The second factor was dosage of N anorganic fertilizer consisted four levels 0, 50, 100 and 150 kg N ha-1. The results showed that there is interaction found between diazotroph endophytic bacteria and N inorganic fertilizer on population of diazotroph endhophytic bacteria  but there were not interaction effect on N concentration and dry matter of red chili plant. Independenly, treatment giving diazotroph endophytic bacteria and N anorganic fertilizer showed not significant effect on N concentration of plant. While the treatment of diazotroph endophytic bacteria and 150 kg N ha-1 inorganic fertilizer were independenly each could significantly increased dry matter of red chili plant on Inceptisols from Jatinangor.           Keywords: Diazotroph endophytic bacteria, N fertilizer, Red chili plant
KARAKTERISTIK PERTUMBUHAN DAN WAKTU GENERASI ISOLAT Azotobacter sp. DAN BAKTERI ENDOFITIK ASAL EKOSISTEM LAHAN SAWAH Mieke Rochimi Setiawati; Pujawati Suryatmana; Diyan Herdiyantoro; Zahra Ilmiyati
Jurnal Agroekoteknologi Vol 6, No 1 (2014)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.49 KB) | DOI: 10.33512/j.agrtek.v6i1.222

Abstract

Azotobacter sp. and N2-fixation endophytic bacteria are microbes that play a role in fixation the N2 from the free air and helps supply of N to plants and can make efficient use of N derived from inorganic fertilizer. N2-fixation microbes utilization in rice fields can reduce the cost of production of rice plants. By studying the characteristics of the two types of beneficial bacteria that is expected to be engineered to enhance the role of these bacteria contribute in supplying N to rice crops. The method used in this study is exploratory and observe the characteristics of growth and generation time of bacteria for 72 hours. The results of this study indicate that the bacterial isolates of Azotobacter sp-1 has the highest population in 48 hours with a total population of 1.58 x107 cfu ml-1 , whereas bacteria Azotobacter sp-2 has the highest population in 60 hours with a total population of 3.12 x106 cfu ml-1 . Both N2-fixation endophytic bacteria populations reached the highest peak in the 60th hour with a total population of N2-fixation endophytic bacteria-1 at 1.12 x 108 cfu ml-1 of N2-fixation endophytic bacteria-2 of 9.4 x 107 cfu ml-1 of both isolates endophytic bacteria showed a similar growth pattern. Azotobacter sp. isolates from rhizosfir of the rice plant have a difference generation time, while the two isolates of N2-fixation endophytic bacteria from tissue of rice plants having the same generation time. Isolates of Azotobacter sp.-1 had the fastest of generation time, capable of generating new cells every 158.66 minutes or every 2.64 hours.
Uji Viabilitas Isolat Bakteri Penambat Nitrogen Halotoleran pada Komposisi Bahan Pembawa yang Berbeda Nida Uli Al-Azmiya; Fiqriah Hanum Khumairah; Mieke Rochimi Setiawati; Tualar Simarmata
Jurnal Agroekoteknologi Vol 13, No 1 (2021)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v13i1.12165

Abstract

The process of microbial inoculation into plant biomass which will be decomposed needs a suitable carrier material to facilitate the application of the microorganisms to be used. Halophilic bacteria are a type of microorganism that can survive high salt levels by maintaining an osmotic balance. The bacterial consortium is a collection of bacteria that work together to form a community, to produce a significant product. The experiment aims to determine the effect of carrier composition on inoculant viability. The experiment was carried out at the laboratory CV. Bintang Asri Arthauly Bandung, West Java, Indonesia. The study used a non factorial randomized design with three replications. The treatment design to be tested in this experiment, among others: c1: Peat 50% + Compost 50% + Nutrition 0%, c2: Peat 50% + Compost 45% + Nutrition 5%, c3: Peat 50% + Compost 40% + Nutrition 10%, c4: Peat 50% + Compost 25 % + Biochar 25% + Nutrition 0%, c5: Peat 50% + Compost 22.5% + Biochar 22.5% + Nutrition 5%, c6: Peat 50% + Compost 20% + Biochar 20% + Nutrition 10%, c7: Peat 50% + Compost 20% + Biochar 20% + Dolomite 5% + Guano 5% + Nutrition 0%, c8: Peat 50% + Compost 17.5% + Biochar 17.5% + Dolomite 5% + Guano 5 % + Nutrition 5%, c9: Peat 50% + Compost 15% + Biochar 15% + Dolomite 5% + Guano 5% + Nutrition 10%. The results of the second stage experiment showed that the c8 composition had the best viability in all carrier compositions.
Co-Authors . Purwanto Abdul Hasyim Sodiq Abraham Suriadikusumah Adawiyah, Aliya Z Ade Setiawan Adhitiya Rana Adinata, Kustiwa Adinata, Kustiwa Aditya, Fasa Afrilandha, Nandha Agung Karuniawan Aisyah, Ayu Siti Alia Halimatusy Alin Kusumah Dewi Alyani Shabrina Amalia Chusnul Anas Ramdhani Anggi Jingga Anne - Nuraini ANNE NURBAITY Anne Nurbaity Anni Yuniarti Apong Sandrawati Arafah, Mayang Sunduz Ari Abdulah Safari Asmiran, Priyanka Azwari, Fachruddin Benang Purwanto Benny Joy Benny Joy Benny Joy Betty N. Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Chotimah, Andina Damayani, Maya Daud Siliwangi Saribun Dedeh H Arief Dedeh H. Arief Dedi Widayat Dewi, Yeni Wispa Diana Nafitri Cahayaningrum Diky Indra Wibawa Dirga Sapta Sara Diyah Sri Utami Diyan Herdiyantoro Diyan Herdiyantoro DIYAN HERDIYANTORO Diyan Herdiyantoro Djuwansah, Muhamad Rachman DWI ANDREAS SANTOSA DWI NINGSIH SUSILOWATI DWI NINGSIH SUSILOWATI Eka Safitri Eko Pranoto Eko Pranoto Eko Pranoto Ellin Harlia Emma Trinurani Sofyan Eulis Tanti Marlina Evi Entang Fatimah Evi Entang Fatimah Fani Fauziah Fani Fauziah, Fani Fatantia Husnaeni Fera Siti Meilani Fiky Yulianto Wicaksono Fitriatin, Betty N Gordon Pius Marihot Halimah, Ummu ‘Azizah Hari R Harlyadi Haryo Probo Kusumo Herdiantoro, Diyan Herlianti, Anissa Mutiara Hersanti - Hindersah, Raginawanti I. Syarifain, Roby Iin Handayani Ilyas, Ichsan Imas Komalasari Indrawati - Indrawati Iskariman, Ahlan Azman Isna Niar Rahmatul Azizah Jajang Sauman Hamdani Kaffah, Ruhnayati Kamluddin, Nadia Nuraniya Khoiriyyah, Luluatul Khumairah, Fiqriah Hanum Kmaluddin, Nadia Nuraniya Kustiwa Adinata Kustiwa Adinata Latifah, Tipah Leoni Silvia Lia Nur Linda Lubis, Ary Satria Maharani, Nadhira Saniya Mahdi Argawan Putra Mahfud Arifin Manurung, Dahlia Florencia Maulana, Nurzen Maya Damayani Maya Damayanti Mayang Sunduz Arafah Melani . Melani Melani Muhamad Khais Prayoga Muhamad Khais Prayoga Nabila Syifa Ariani Nadia Nuraniya Kamaluddin Nadia Nuraniya Kamluddin Nanda Aditya Setyawan Neni Rostini Neni Rostini Neni Rostini Nenny Nurlaeny Nida Uli Al-Azmiya Nizar Ulfah Nugraha, Gita Bina Nurmayulis Nurullita Fitri Qurnia Pirda Nurhopipah Prayoga, Muhamad Khais Prayoga, Muhamad Khais Priyanka Asmiran Pujawati Suryatmana Putra Utama Putri Sri Judiani Purba Rachelita, Nadia Rahma Tia Harahap Reginawanti Hindersah Reginawati Hindersah Restu Wulansari Restu Wulansari, Restu Rhazista Noviardi Richard A. Gunawan Ridha Hudaya Ridha Hudaya Rija Sudirja Risanti, Rara Rahmatika Salsabilla, Camilla Shabrina Rahma Fauzia Shinta Nurrizqi Indrayani Silke Stoeber Silmi Rahadiana Putri Stoeber, Silke Stoeber, Silke Sumadi Sumadi Suryatma, Pujawati Susiyanti, Susiyanti Syifa Nabila Kurnia Tessa Novianty Putri Asova Tien Turmuktini Tualar Simarmata Tualar Simarmata Tualar Simarmata Tualar Simarmata Tualar Simarmata Tualar Simarmata Tualar Slmarmata Ummu ‘Azizah Halimah Utami, Deswita Uum Umiyati Wati, Dyah Aditya Yanti Ningtyas, Dewi Nurma Yati Rachmiati Yati Rachmiati, Yati Yori Tridendra Yudith Silfani Yuli Astuti Hidayati Yuliati Machfud Yuyun Sumarni Zaenal Mutaqin Zahra Ilmiyati