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Utilization of Fobio and Streptomyces sp. in Improving the Growth of Shallots Sri Wiyatiningsih; Penta Suryaminarsih; Gusdino Hasyidan
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1507

Abstract

Shallots (Allium ascalonicum L.) is a spice vegetable commodity that is needed by the community as a cooking spice to increase the taste of eating pleasure and for traditional medicines that are efficacious for health. Control using chemicals is believed to be effective in overcoming pathogen attacks, but the continuous addition of chemical pesticides will have a negative impact on the environment and consumers. To deal with the negative impact of the use of these chemicals, namely by applying a sustainable farming system that was originally a conventional farming system and includes organic agriculture (Fobio biopesticide application) and biological (Application of Streptomyces sp. biological agents). The purpose of this study was to determine the effectiveness of the formulation of the biopesticide Fobio and the biological agent Streptomyces sp. to increase the growth of leeks and minimize the negative impact of using fungicides in Bulugunung Village, Plaosan District, Magetan Regency but still have the potential for maximum harvest success. The study was conducted from March to May 2021. This study used a Completely Randomized Design (CRD) consisting of 4 treatments with 6 replications. Each experimental unit consisted of 100 plants. Observation parameters consisted of leaf height and number of leaves of shallot plants. The results showed that the application of biopesticide Fobio and biological agents Streptomyces sp. did not significantly affect the height of the shallot plant and had a significant effect on the number of leaves of the shallot plant
Pathogenicity of Entomopathogenic Nematodes (NEP) Results of In Vitro Propagation Using Egg Yolk Media Against Mustard Caterpillar Plutella xylostella Mohamad Hipti; Wiludjeng Widajati; Sri Wiyatiningsih; Ramadhani Mahendra Kusuma
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1509

Abstract

Decreased mustard plant production is inseparable from the presence of plant pests and disease, one of which is Plutella xylostella. This pest can reduce the yield by 30-40%, even in some cases found to experience crop failure. Generally, in the community, controlling pests is using chemical pesticides. However, excessive use of chemical pesticides harms the environment and triggers health problems in humans, So, biological control is needed. One of them is an entomopathogenic nematode (NEP). Advantages Nematodes are environmentally friendly and are fast in crippling their host, but the multiplication of entomopathogenic nematodes encounters many obstacles, so research on these alternatives is needed. This research was carried out to determine the pathogenicity of entomopathogenic Nematodes cultured from the egg yolk media on Plutella xylostella larvae, then developed as an effective biocontrol. The pathogenicity test for Plutella xylostella larvae was performed using nine treatments placed in a factorial Completely Randomized Design (CRD) with each treatment repeated three times. The study indicate that the four bacterial isolates had the same ability to kill Plutella xylostella larvae during four days of observation, with the greatest percentage of 100 percent and the lowest percentage of 76.7 percent.
KEANEKARAGAMAN ARTHROPODA PADA TANAMAN BAWANG MERAH DI LAHAN YANG DIAPLIKASI FORMULASI BIOPESTISIDA DI KABUPATEN NGANJUK Hendra Wahyudi Ariyono; Sri Wiyatiningsih; Wiwin Windriyanti
Plumula : Berkala Ilmiah Agroteknologi Vol 10 No 1 (2022): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v10i1.73

Abstract

Shallots (Allium cepa L.) are one of the leading vegetable commodities that have been intensively cultivated by farmers for a long time. A large percentage of damage to plants can result in reduced tuber weight. Study of insect diversity is the first step in pest control and pest management in crops. The purpose of this study, namely, to determine the diversity index of pests and natural enemies in shallot plantations after the use of biopesticide and chemical pesticide formulations. The research was carried out from December 2020 to February 2021. The study was conducted in Sukorejo Village, Rejoso District, Nganjuk Regency with an altitude of 140 m above sea level where fobio (biopesticide formulation) and chemical insecticides were applied. The area of the research area is about 140 m², where there are 2,400 shallot plant populations. This study used a non-factorial randomized block design (RAK) method. There were 4 treatments which were repeated 6 times to obtain 24 experimental units. Each experimental unit consisted of 100 shallot plants with a spacing of 18 x 14 m and a barrier distance of 50 cm. The results showed. Based on observations, the total number of arthropods found in shallot plantations in 5 observations was 1391 individuals consisting of seven orders of insects and one order of polypods. The highest diversity index was in treatment B (semi organic) because the combination of NPK fertilizer and spraying fobio on plants could increase insect diversity.
Combination of FOBIO biopesticide and Brassica rapa L. as remediator of heavy metal Pb in soil Surya Ari Widya; Moch. Arifin; Sri Wiyatiningsih
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 5, No 1 (2022)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2022.005.01.6

Abstract

Marginal soil is infertile soil caused by various factors, one of which is the excessive accumulation of heavy metals. FOBIO microorganism-based biopesticide formula containing phosphate solubilizing bacteria is expected to be used to reduce excess heavy metals in the soil. Brasica rapa L. is a variety resistant to Pb heavy metal stress and absorbs more heavy metals into root tissue than leaf tissue with the help of microorganisms. This study aimed to determine the combination of Brassica rapa L. and FOBIO biopesticide in absorbing Pb in the soil, determine the growth of Brassica rapa L. at each Pb concentration, and determine the population of phosphate-solubilizing bacteria in the soil at each level of Pb stress after remediation. This study used variations in the concentration of heavy metals, particularly Pb. Parameters observed included plant growth, Pb concentration in the soil before and after remediation, and the population of phosphate-solubilizing bacteria. The results showed that combining FOBIO biopesticides and Brassica rapa L. could reduce Pb concentration in the soil. The growth of Brassica rapa L. plants was significantly different after treatment. The findings confirmed that increasing, the concentration of Pb led to a decrease in the population of phosphate-solubilizing bacteria.
Effectiveness of Lettuce Seed Encapsulation Containing Trichoderma Sp. in Control of Damping-off Disease Sarah Hikmah Marieska; Sri Wiyatiningsih; Herry Nirwanto
Journal of Applied Agricultural Science and Technology Vol. 6 No. 2 (2022): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.682 KB) | DOI: 10.55043/jaast.v6i2.66

Abstract

Utilization of the antagonist fungus Trichoderma sp. in suppressing damping-off disease caused by Rhizoctonia solani can be done in various ways. One of them is through coating the seeds or encapsulation. This study aims to determine the effectiveness of lettuce seeds encapsulation containing Trichoderma sp. in suppressing damping-off disease through storage time of up to 12 weeks. The results showed that the use of talc and kaolin as a material carrier in encapsulation with 1 week of storage had the highest germination rate of 96%, while the lowest germination was at 12 weeks of storage, which was only 0-5%. The use of talc carrier showed a low percentage of infected seedlings, starting from storage time of 0 to 8 weeks. At 8 weeks of storage, the provision of carrier material in the form of talc showed the lowest percentage of infected seedlings, which was 40% on the last day of observation and had the highest value of effectiveness in controlling Rhizoctonia solani damping-off disease, which was 60%. Thus, seed encapsulation using a talc carrier was the most effective in suppressing damping-off disease up to 8 weeks of storage
VIABILITAS TRICHODERMA SP. PADA ENKAPSULASI BENIH SELADA DALAM BEBERAPA MASA PENYIMPANAN Sarah Hikmah Marieska; Sri Wiyatiningsih; Herry Nirwanto
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 7, No 3 (2022): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v7i3.7012

Abstract

Trichoderma sp. diketahui memiliki spektrum pengendalian yang luas, serta memiliki sifat antagonistik yang kuat dalam menghambat pertumbuhan jamur patogen. Enkapsulasi benih merupakan teknik pelapisan benih yang dapat diterapkan untuk spora jamur dan sel bakteri. Penelitian ini dilakukan untuk mengetahui bahan pembawa yang paling baik dalam meningkatkan kemampuan antagonis jamur Trichoderma sp. melalui beberapa masa penyimpanan. Hasil pengujian in vitro berdasarkan pengukuran diameter koloni menunjukkan bahwa Trichoderma sp. pada semua perlakuan enkapsulasi yang diujikan rata-rata memiliki viabilitas yang tinggi. Berdasarkan pengamatan jumlah spora, terdapat pengaruh yang berbeda nyata pada tiap perlakuan. Kerapatan spora tertinggi terdapat pada perlakuan bahan pembawa biochar dengan penyimpanan 4 minggu dan 8 minggu. Sementara kerapatan spora terkecil terdapat pada perlakuan bahan pembawa kaolin dengan lama penyimpanan 12 dan bahan pembawa talc dengan lama penyimpana 0 minggu. Dengan demikian, penggunaan bahan pembawa biochar mampu menambah viabilitas Trichoderma sp. hingga waktu penyimpanan 8 minggu.
POTENSI Trichoderma spp. SEBAGAI ANTAGONIS PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) SECARA IN VITRO Alfia Rofika Sari; Endang Triwahyu Prasetyawati; Sri Wiyatiningsih
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 7, No 3 (2022): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v7i3.6986

Abstract

Penyakit layu bakteri disebabkan oleh bakteri Ralstonia solanacearum. Memiliki jangkauan tanaman inang yang luas termasuk famili Solanaceae. Kerugian yang ditimbulkan sangat besar hingga mencapai 100% pada tanaman budidaya. Pengendalian alternatif ramah lingkungan yaitu dengan menggunakan agensia hayati Trichoderma sp. Beberapa jenis Trichoderma sp. yang diketahui mampu mengendalikan R. solanacearum antara lain Trichoderma harzianum, Trichoderma asperellum, dan  Trichoderma koningii. Penelitian ini bertujuan untuk mengetahui potensi Trichoderma spp dalam mengendalian R. solanacearum diluar tanaman inang dengan lingkungan yang terkontrol. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu jamur antagonis. Percobaan yang dilakukan yaitu tanpa jamur antagonis sebagai kontrol, T. harzianum, T. asperellum, dan T. koningii dengan 5 kali pengulangan. Hasil penelitian menunjukkan bahwa dari ketiga jenis Trichoderma spp yang mampu menghambat paling besar yaitu pada perlakuan T. asperellum dengan rerata daya hambat mencapai 9,6 mm pada umur 24 jam dan 6,8 mm pada umur 48 jam.
The Utilisation of Botanical Pesticide as Pest Control in The Management of Organic Vegetable Farming Faradila Amir; Sri Wiyatiningsih
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3115

Abstract

Botanical pesticides are eco-friendly pesticides derived from plant extracts. Botanical pesticides can be used as pest control in organic vegetable farming. Some pests are known to attack organic vegetables, such as the large cabbage-heart caterpillar (Crocidolomia pavonana) and aphids (Myzus persicae) attack the cauliflower plant; grasshopper (Atractomorpha similis) and flea beetle (Phyllotreta nigripes) attack the green mustard plant, and the beetle (Epilachna borealis) attacks tomato plants. Some botanical pesticides used to control these pests are derived from soursop leaf extract, paitan leaf extract, and neem fruit extract. The results of the application of these pesticides can reduce the pest population; the percentage of pest population decline in C. pavonana is 30%; M. persicae s by 65.5%; A. similis by 66.6%; Ph. nigripes by 60%; and E. borealis as much as 66.6%.
Pengaruh Biopestisida Fobio dan Agens Hayati Trichoderma sp., terhadap Penyakit Layu Fusarium pada Bawang Merah Farisa; Dita Megasari; Sri Wiyatiningsih
Agriprima : Journal of Applied Agricultural Sciences Vol 7 No 1 (2023): MARCH
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v7i1.522

Abstract

Pengendalian penyakit tanaman bawang merah hingga saat ini masih mengandalkan fungisida kimia yang dapat mencemari lingkungan, sehingga perlu dilakukan pengendalian secara hayati dan ramah lingkungan. Alternatif yang dapat diterapkan adalah menggunakan Biopestisida Fobio dan agens hayati Trichoderma sp. Tujuan penelitian ini adalah untuk mengetahui respon aplikasi Biopestisida Fobio dan agens hayati Trichoderma sp. terhadap penyakit layu fusarium pada bawang merah. Penelitian dilaksanakan pada bulan Agustus-Oktober 2022 di Kecamatan Kedopok, Kota Probolinggo. Penelitian menggunakan Rancangan Petak Terbagi dengan dua faktor, yaitu konsentrasi Biopestisida Fobio terdiri dari 3 taraf yaitu petak utama adalah F0 (kontrol atau perlakuan pestisida kimia), F1 (Fobio 5 ml/liter), F2 (Fobio 7,5 ml/liter) dan anak petak yaitu konsentrasi Trichoderma sp. terdiri dari 3 taraf yaitu T0 (kontrol atau perlakuan pestisida kimia), T1 (Trichoderma sp. 10 ml/liter), T2 (Trichoderma sp. 20 ml/liter) sehingga terdapat 9 kombinasi perlakuan. Parameter pengamatan meliputi periode inkubasi, kejadian penyakit, berat basah umbi, dan berat kering umbi. Hasil penelitian dari semua kombinasi perlakuan tidak berbeda nyata. Hasil perlakuan aplikasi Biopestisida Fobio menunjukkan bahwa tanaman bawang merah berbeda nyata terhadap periode inkubasi pada perlakuan F2 dengan nilai rata-rata tertinggi yaitu 20 hst. Kejadian penyakit terendah terdapat pada perlakuan F2 pada umur 28 dan 42 hst. Perolehan berat basah paling tinggi diperoleh pada perlakuan F2T0 yaitu 2,128 kg. Perolehan berat kering tertinggi yaitu pada perlakuan F0T2.
Response of Microorganism Suspension and Various Kinds of Organic Fertilizers to the Development of Pathogen Fusarium sp. on Shallots Ratna Arifiani Sukmaningrum; Sri Wiyatiningsih; Endang Triwahyu Prasetya; Retno Sulistiyowati
Jurnal Agrinika: Jurnal Agroteknologi dan Agribisnis Vol 7 No 1 (2023): MARCH
Publisher : Kadiri University - Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/agrinika.v7i1.4137

Abstract

Shallot (Allium ascalonicum L) is one of the horticultural plants that acts as a natural antioxidant. Shallot's productivity in Indonesia is relatively low. It is influenced by various factors, one of which is due to the attack of a soil-borne pathogen, namely Fusarium oxysporum. Symptoms of Fusarium oxysporum pathogen attack are yellow or pale green leaves and more elongated growth. Severe attack leads plant to death. Balanced fertilization and biopesticides application can prevent Fusarium wilt. This study aims to reduce and control Fusarium wilt disease. The research method used was the Split Plot Design method which consisted of two factors with the main plot, namely the application of suspension of microorganisms (S) which consists of two levels, namely fungicide application (S0) and Fobio application and the sub-plot, namely the application of various organic fertilizers (M) which consists of three levels, namely control (chemical fertilizer) (M0), chicken manure (M1), and Piensbio organic fertilizer (M2). Observation data for each treatment will be analyzed using analysis of variance (ANOVA) followed by the Duncan test (alpha 5%) if there were differences in each treatment. The results of this study, the main plot treatment, fungicide application (S0), showed an incubation period of 29 days and an average intensity of disease attacks of 4.2%. The treatment of subplots, a type of organic fertilizer (M), is unable to suppress the intensity of the attack and slow down the incubation period of the Fusarium sp on shallot crops. There was no interaction between the treatment of the main plots and the subplots on all the observation parameters.
Co-Authors Agustin, Indah Sari Dwi Alfia Rofika Sari Arika Purnawati Armadianty, Fany Risma Boleng, Maya Stolastika Deviyanti, Nur Qomariyah Dewi Anggraini Diasi, Ananta Rizki Didik Utomo Pribadi Dinda Rizka Rahayu Dita Megasari Dwi Afrian Dwi Afrian Dwi Suci Rahayu Eben, Philipus Wahyu Baskoro Eko Setiyo Wawan Elvira, Nolla Dwi Endang Triwahyu Prasetya Endang Triwahyu Prasetyawati Ersyaf, Ihza Muhammad F. Deru Dewanti Fajri Rizky Saptono Faradila Amir Farisa Fitri Wijayanti Fitriya Pebriana Gusdino Hasyidan Gusdino Hasyidan Harijani, Wiwik Sri Haykal, Arkan Hendra Wahyudi Ariyono Hendra Wahyudi Ariyono Herry Nirwanto Hery Nirwanto, Hery Ika Nur Fitriana Indah Anita Sari Izza, Salsa Bila Ari Nur Kurniawan, Muhammad Izza Laila, Nita Nur Laily, Nurul Larrisa, Damasa Ines Lia Iswindari Mukaromah Maroeto ., Maroeto Maroeto Maroeto Moch Nur Yudha Moch. Arifin Mohamad Hipti Nabila Alysia Multazam Nabila Naylofar Nauval Zakqy Nia Rulinggar Putri M Pangesti Nugrahani Penta SURYAMINARSIH Penta Suryaminarsih Pratama Wirya Atmaja Pribadi, Didik Utomo Ramadhani Mahendra Kusuma Rani, Sasiska Ratna Arifiani Sukmaningrum Retno Sulistiyowati Riko Setya Wijaya Robithotul Ummah Rosyiidah Sholihin, Alyaa Arwaa Santoso, Wahyu Sarah Hikmah Marieska Sarah Hikmah Marieska Sari, Nadya Naurah Sentoso, Wahyu Septikahady, Hani Shafa, Yola Salsabila Shavira Dwi Livita Reza Sigit Dwi Nugroho Simanjuntak, William Fernando Sisko Budianto Siti Fatimatus Syahrok Surya Ari Widya Suryandika, Fadila Suwandi Syahrok, Siti Fatimatus Tri MUJOKO Tri Mujoko Tri Wahyu Prasetyawati, Endang Vikayanti Vikayanti Wahyu Santoso Wahyu Santoso Wahyu Santoso Wanti Mindari Widyananda, Kanaya Syafina Wijaya, Riko Setya Wiludjeng Widajati Wiludjeng Widayati Wiludjeng Widiyati Windriyanti, Wiwin Wiwik Sri Harijani Wiwik Sri Harijani Yenny WURYANDARI