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Bioencapsulation of Streptomyces sp. with Sodium Alginate for Control Fusarium sp. in Shallot Plants Widyananda, Kanaya Syafina; Purnawati, Arika; Wiyatiningsih, Sri
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.67882

Abstract

This study investigated the effects of Streptomyces sp. encapsulated in sodium alginate beads on the growth and health of shallot (Allium cepa L.) plants. Different sodium alginate concentrations (2%, 2.5%, and 3%) and application timings (7 days before transplanting and at transplanting) were tested. The mass and diameter of the beads showed slight decreases over storage time, indicating some shrinkage but maintaining structural integrity to protect the bacteria. Encapsulation efficiency was highest at 3% sodium alginate, which also correlated    with    increased    bead     stability.     Viability     tests     revealed that Streptomyces maintained high colony-forming units (CFU) across the treatments, confirming the protective effect of the encapsulation. Application of beads significantly reduced Fusarium wilt disease intensity and improved bulb weight. Treatments with higher sodium alginate concentrations and earlier application times showed the most pronounced benefits. These findings demonstrate that beads using optimal sodium alginate concentration and timing enhance microbial viability and biological control efficacy, contributing to healthier and more productive shallot cultivation.
Biodegradation Potential of Streptomyces sp. Against propineb-Based 70% Fungicide Under In Vitro Conditions Sari, Nadya Naurah; Mujoko, Tri; Wiyatiningsih, Sri
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.66871

Abstract

The intensive use of propineb-based fungicides in agriculture can negatively affect the environment due to toxic and persistent residues in soil. An environmentally friendly approach to reduce these impacts is bioremediation using microorganisms such as Streptomyces sp. This study aimed to examine the tolerance and biodegradation potential of Streptomyces sp. toward 70% propineb fungicide under in vitro conditions. The research was conducted at the Plant Health 1 Laboratory, Universitas Pembangunan Nasional “Veteran” East Java, from June to August 2025. The experiments were carried out in two stages: (1) a tolerance assay on Glucose Nutrient Agar containing propineb at 0; 0,50; 1,00; 1,50; and 2,00 g/L, and (2) a degradation assay using a dual-culture method with Colletotrichum sp. as a bioindicator. Observation variables included Total Plate Count (TPC) of Streptomyces sp., colony diameter of Colletotrichum sp., and the relative inhibition level (THR). Results showed that Streptomyces sp. was able to grow at all fungicide concentrations. The highest population was found in P0 (0 g/L) with 1,42×1013 CFU/mL with growth decreasing as propineb concentration increased, reaching the lowest level of 3,22×10¹⁰ CFU/mL in P4 (2.00 g/L). In the degradation assay, Colletotrichum sp. colony diameter decreased from 3,38 cm in P0 (0 g/L)  to 2,24 cm in P4 (2,00 g/L). Meanwhile, THR values increased consistently with fungicide concentration, rising from 3,17% in P0 (0 g/L)  to 35,48% in P4 (2,00 g/L).
Effectiveness of Fobio Biopesticide Concentration and Soil Sterilization Interval for Controlling Stem Rot Disease by Fusarium oxysporum in Vanilla planifolia Laily, Nurul; Wiyatiningsih, Sri; Purnawati, Arika
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8381

Abstract

Background: Stem rot disease caused by Fusarium oxysporum is one of the major diseases affecting vanilla plants (Vanilla planifolia A.), which can cause losses of up to 100%. Current management practices rely heavily on synthetic pesticides and often lack precision, raising environmental concerns and need for sustainable disease management strategies. This study aimed to determine the effectiveness of Fobio biopesticide concentration and soil sterilization intervals as control methods in suppressing the development of vanilla stem rot disease. Methodology: The study was conducted in vivo in Wonosalam Village, Jombang Regency using a factorial completely randomized design (CRD) with factors of Fobio biopesticide concentration (5 mL/L, 10 mL/L, and 15 mL/L) and application interval (1-week and 2-week intervals). The data were analyzed statistically using the Analysis of Covariance (ANCOVA). The parameters observed included disease intensity, infection rate, and biopesticide effectiveness. Findings: The results showed that the initial disease intensity had a very significant effect on the final intensity. The treatment with an application interval of once a week reduced disease intensity to 23.89%, lower than the interval of 2-weeks (26.67%) and the control (46.66%). A concentration of 15 ml/L reduced disease intensity to 24.94%, with the highest effectiveness also obtained in the 15 ml/L treatment at 1-week intervals (50.29%). The interaction between interval and concentration was also not significant, indicating that the effect of concentration did not change depending on treatment interval. The biopesticide Fobio was found to be sufficiently effective in suppressing the development of vanilla stem rot, and applications with more frequent intervals showed the highest efficacy. Contribution: This study highlights the importance of integrating biopesticide concentration  and application interval while adjusting for initial disease intensity in vanilla stem rot management.
ISOLASI, IDENTIFIKASI MORFOMETRIK, DAN POTENSI NEMATODA ENTOMOPATOGEN Heterorhabditis americana DARI LAHAN TEBU DI KEDIRI, INDONESIA SEBAGAI KANDIDAT BIOPESTISIDA RAMAH LINGKUNGAN: ISOLATION, MORPHOMETRIC IDENTIFICATION, AND POTENTIAL OF THE ENTOMOPATHOGENIC NEMATODE Heterorhabditis americana FROM SUGARCANE FIELDS IN KEDIRI, INDONESIA AS AN ENVIRONMENTALLY FRIENDLY BIOPESTICIDE CANDIDATE Simanjuntak, William Fernando; Kusuma, Ramadhani Mahendra; Wiyatiningsih, Sri
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 14 No. 1 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2026.014.1.4

Abstract

Nematoda entomopatogen (NEP) adalah organisme mikroskopis berbentuk mirip cacing yang memiliki potensi besar sebagai agens pengendali hayati. Kediri merupakan salah satu daerah penghasil tebu utama di Indonesia. Nematoda Heterorhabditis mampu membunuh uret (Lepidiota stigma) sebagai hama utama tebu, sehingga menunjukkan potensi yang besar bagi Heterorhabditis sp. sebagai musuh alami hama tanaman tebu. Penelitian ini bertujuan untuk mengeksplorasi keberadaan Heterorhabditis sp. di lahan tebu Kediri melalui pengambilan sampel tanah dan identifikasi morfometrik. Eksplorasi Heterorhabditis diawali dengan pengambilan sampel tanah pada tanaman tebu sehat kemudian dilakukan baiting NEP. Nematoda entomopatogen yang diperoleh diisolasi menggunakan metode white trap dan difiksasi sebelum diidentifikasi. Hasil penelitian menunjukkan bahwa pada tanah lahan tebu di Kediri terdapat nematoda Heterorhabditis sp. Nematoda Heterorhabditis sp. yang ditemukan memiliki panjang tubuh 467,6 µm, Lebar tubuh 26,9 µm, panjang bulbus basal 13,7 µm, dan panjang ekor 36,2 µm. Nematoda entomopatogen yang diperoleh berasal dari genus Heterorhabditis dengan dugaan spesies Heterorhabditis americana. Spesies ini berpotensi untuk dikembangkan dalam berbagai formulasi biopestisida guna mendukung pengembangan Pengelolaan Hama Terpadu (PHT) di Indonesia.
Application of Biopriming Using Secondary Metabolites of Beauveria bassiana in Reducing the Intensity of Fusarium Wilt Disease in Chili Plants Nabila Naylofar; Sri Wiyatiningsih; Tri Mujoko; Vikayanti Vikayanti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.8385

Abstract

Background: Fusarium wilt, caused by Fusarium sp., is one of the main obstacles to chili pepper (Capsicum frutescens L.) production. Disease control using chemical pesticides and fungicides is considered effective in agricultural fields, but excessive use can result in environmental contamination and health risks, including cancer. This makes the application of biological control an effective strategy in overcoming Fusarium wilt disease in chili plants, especially in increasing plant productivity. This study aims to test the effectiveness of biopriming using Beauveria bassiana secondary metabolites in suppressing Fusarium wilt infection in chili seeds in vivo. Methodology: This study used a Completely Randomized Design (CRD). Secondary metabolites were applied to chili seeds at four concentration levels (15%, 25%, 35%, and 45%) as well as positive and negative controls. The treated seeds were planted in a medium inoculated with pathogens and observed for nine weeks. Disease intensity was recorded every seven days and analyzed using ANOVA followed by Tukey's Honestly Significant Difference (HSD) test at the 5% level. Findings:  The results of the variance test 49–63 days after sowing (DAS) showed significant differences between treatments; a further HSD test was conducted at a 5% level. The application of Beauveria bassiana secondary metabolites as a biopriming agent on chili seeds is considered to be ineffective. Contributions: This study provides the first in vivo evidence on the application of Beauveria bassiana secondary metabolites as a seed biopriming agent to suppress Fusarium wilt in chili plants, thereby advancing preventive biocontrol strategies and contributing to the development of environmentally sustainable plant disease management
Co-Authors Agustin, Indah Sari Dwi Alfia Rofika Sari Arika Purnawati Armadianty, Fany Risma Boleng, Maya Stolastika Deviyanti, Nur Qomariyah Dewi Anggraini Diasi, Ananta Rizki Didik Utomo Pribadi Dinda Rizka Rahayu Dita Megasari Dwi Afrian Dwi Afrian Dwi Suci Rahayu Eben, Philipus Wahyu Baskoro Eko Setiyo Wawan Elvira, Nolla Dwi Endang Triwahyu Prasetya Endang Triwahyu Prasetyawati Ersyaf, Ihza Muhammad F. Deru Dewanti Fajri Rizky Saptono Faradila Amir Farisa Fitri Wijayanti Fitriya Pebriana Gusdino Hasyidan Gusdino Hasyidan Harijani, Wiwik Sri Haykal, Arkan Hendra Wahyudi Ariyono Hendra Wahyudi Ariyono Herry Nirwanto Hery Nirwanto, Hery Ika Nur Fitriana Indah Anita Sari Izza, Salsa Bila Ari Nur Kurniawan, Muhammad Izza Laila, Nita Nur Laily, Nurul Larrisa, Damasa Ines Lia Iswindari Mukaromah Maroeto ., Maroeto Maroeto Maroeto Moch Nur Yudha Moch. Arifin Mohamad Hipti Nabila Alysia Multazam Nabila Naylofar Nauval Zakqy Nia Rulinggar Putri M Pangesti Nugrahani Penta Suryaminarsih Penta SURYAMINARSIH Pratama Wirya Atmaja Pribadi, Didik Utomo Ramadhani Mahendra Kusuma Rani, Sasiska Ratna Arifiani Sukmaningrum Retno Sulistiyowati Riko Setya Wijaya Robithotul Ummah Rosyiidah Sholihin, Alyaa Arwaa Santoso, Wahyu Sarah Hikmah Marieska Sarah Hikmah Marieska Sari, Nadya Naurah Sentoso, Wahyu Septikahady, Hani Shafa, Yola Salsabila Shavira Dwi Livita Reza Sigit Dwi Nugroho Simanjuntak, William Fernando Sisko Budianto Siti Fatimatus Syahrok Surya Ari Widya Suryandika, Fadila Suwandi Syahrok, Siti Fatimatus Tri Mujoko Tri MUJOKO Tri Wahyu Prasetyawati, Endang Vikayanti Vikayanti Wahyu Santoso Wahyu Santoso Wahyu Santoso Wanti Mindari Widyananda, Kanaya Syafina Wijaya, Riko Setya Wiludjeng Widajati Wiludjeng Widayati Wiludjeng Widiyati Windriyanti, Wiwin Wiwik Sri Harijani Wiwik Sri Harijani Yenny WURYANDARI