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Application of Biopriming Using Secondary Metabolites of Beauveria bassiana in Reducing the Intensity of Fusarium Wilt Disease in Chili Plants Nabila Naylofar; Sri Wiyatiningsih; Tri Mujoko; Vikayanti Vikayanti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.8385

Abstract

Background: Fusarium wilt, caused by Fusarium sp., is one of the main obstacles to chili pepper (Capsicum frutescens L.) production. Disease control using chemical pesticides and fungicides is considered effective in agricultural fields, but excessive use can result in environmental contamination and health risks, including cancer. This makes the application of biological control an effective strategy in overcoming Fusarium wilt disease in chili plants, especially in increasing plant productivity. This study aims to test the effectiveness of biopriming using Beauveria bassiana secondary metabolites in suppressing Fusarium wilt infection in chili seeds in vivo. Methodology: This study used a Completely Randomized Design (CRD). Secondary metabolites were applied to chili seeds at four concentration levels (15%, 25%, 35%, and 45%) as well as positive and negative controls. The treated seeds were planted in a medium inoculated with pathogens and observed for nine weeks. Disease intensity was recorded every seven days and analyzed using ANOVA followed by Tukey's Honestly Significant Difference (HSD) test at the 5% level. Findings:  The results of the variance test 49–63 days after sowing (DAS) showed significant differences between treatments; a further HSD test was conducted at a 5% level. The application of Beauveria bassiana secondary metabolites as a biopriming agent on chili seeds is considered to be ineffective. Contributions: This study provides the first in vivo evidence on the application of Beauveria bassiana secondary metabolites as a seed biopriming agent to suppress Fusarium wilt in chili plants, thereby advancing preventive biocontrol strategies and contributing to the development of environmentally sustainable plant disease management
Effectiveness of Fobio Biopesticide Concentration Against Stem Rot Disease in Vanilla Plants Winadya, Archamiyanis Putri; Wiyatiningsih, Sri; Mujoko, Tri
CROPSAVER Vol 9, No 1 (2026)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v9i1.67869

Abstract

Fusarium oxysporum is a soil-borne pathogen fungus that causes vanilla stem rot, which reduces vanilla productivity. This study aimed to determine the effectiveness of various concentrations of the biopesticide Fobio  in suppressing vanilla stem rot caused by Fusarium oxysporum. This study was conducted in vivo in Tukum Hamlet, Wonosalam District, Jombang Regency, East Java. This study used a Completely Randomized Design (CRD) with four treatments and five replications consisiting of : 5 ml/L (FA), 7.5 ml/L (FB), 10 ml/L (FC), and Control (K). The results showed that 7.5 ml/L was the best concentration, reducing disease intensity to 12.80%, with a biopesticide effectiveness of 51%.
EFFECTIVENESS TEST OF THE APPLICATION OF THE BIOPESTICIDE FOBIO IN CONTROL OF BROWN SPOT DISEASE (CURVULARIA SP.) ON RICE PLANTS (ORYZA SATIVA L.) IN RICE FIELDS IN PUTON VILLAGE, JOMBANG REGENCY, EAST JAVA Ustman Buchori; Sri Wiyatiningsih; Tri Mujoko
Multidiciplinary Output Research For Actual and International Issue (MORFAI) Vol. 6 No. 4 (2026): Multidiciplinary Output Research For Actual and International Issue
Publisher : RADJA PUBLIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.21912308

Abstract

Brown spot (Curvularia sp.) is a plant disease that poses a serious threat to cultivated crops such as rice. This study aims to test the effectiveness of the biopesticide FOBIO in suppressing the intensity of this disease and supporting rice plant growth. The study was conducted in Puton Village, Jombang, using a factorial randomized block design with a combination of FOBIO concentrations (0, 5, and 10 ml/L) and seed soaking times (10, 20, and 30 minutes). The results showed that FOBIO treatment, especially at a concentration of 10 ml/L for 30 minutes, effectively reduced disease intensity and increased plant growth rates. FOBIO has the potential to be an environmentally friendly alternative solution in an integrated pest control system that can be used in the long term.
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Damasa Ines Larrisa; Pangesti Nugrahani; Sri Wiyatiningsih
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Damasa Ines Larrisa; Pangesti Nugrahani; Sri Wiyatiningsih
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
Co-Authors Agustin, Indah Sari Dwi Alfia Rofika Sari Arika Purnawati Armadianty, Fany Risma Boleng, Maya Stolastika Damasa Ines Larrisa Deviyanti, Nur Qomariyah Dewanti, Felicitas Deru Diasi, Ananta Rizki Dinda Rizka Rahayu Dita Megasari Dwi Afrian Dwi Afrian Dwi Suci Rahayu Dykha Maretha Setyawan Eben, Philipus Wahyu Baskoro Eko Setiyo Wawan Elvira, Nolla Dwi Endang Tri Wahyu Prasetyawati Endang Triwahyu Prasetya Ersyaf, Ihza Muhammad F. Deru Dewanti Fajri Rizky Saptono Faradila Amir Farisa Fitri Wijayanti Fitriya Pebriana Gusdino Hasyidan Gusdino Hasyidan Harijani, Wiwik Sri Hendra Wahyudi Ariyono Hendra Wahyudi Ariyono Herry Nirwanto Hery Nirwanto Ika Nur Fitriana Indah Anita Sari Krisna Bayu Prasetiyo Laila, Nita Nur Lia Iswindari Mukaromah Maroeto ., Maroeto Maroeto Maroeto Moch Nur Yudha Moch. Arifin Mohamad Hipti Mutiara Alyzza Bilqis Nabila Alysia Multazam Nabila Naylofar Nauval Zakqy Nia Rulinggar Putri M Pangesti Nugrahani Penta SURYAMINARSIH Penta Suryaminarsih Prasetyawati, Endang Triwahyu Pratama Wirya Atmaja Pribadi, Didik Utomo Rafi Dwi Nugraha RAMADHANI MAHENDRA KUSUMA Rani, Sasiska Ratna Arifiani Sukmaningrum Retno Sulistiyowati Riko Setya Wijaya Robithotul Ummah Rosyiidah Sholihin, Alyaa Arwaa Santoso, Wahyu Sarah Hikmah Marieska Sarah Hikmah Marieska Sari, Nadya Naurah Sentoso, Wahyu Septikahady, Hani Shafa, Yola Salsabila Shavira Dwi Livita Reza Sigit Dwi Nugroho Simanjuntak, William Fernando Sisko Budianto Siti Fatimatus Syahrok Surya Ari Widya Suryandika, Fadila Suwandi Tri Mujoko Tri MUJOKO Tri Wahyu Prasetyawati, Endang Ustman Buchori Vikayanti Vikayanti Wahyu Santoso Wahyu Santoso Wahyu Santoso Wanti Mindari Widyananda, Kanaya Syafina Wijaya, Riko Setya Wiludjeng Widajati Wiludjeng Widayati Wiludjeng Widiyati Winadya, Archamiyanis Putri Windriyanti, Wiwin Wiwik Sri Harijani Wiwik Sri Harijani Yenny WURYANDARI