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The Potential of Bacillus sp. and FOBIO Biopesticide with Mustard Greens (Brassica rapa L. var. parachinensis) as Bioremediation Agents for Cadmium Dykha Maretha Setyawan; Endang Tri Wahyu Prasetyawati; Sri Wiyatiningsih
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 15 No. 1 (2026): February 2026
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v15i1.1-9

Abstract

Cadmium (Cd) contamination in agricultural land due to excessive use of fertilizers and pesticides has detrimental effects on soil quality, plant growth, and human health. Bioremediation offers a simple and environmentally friendly approach to address this issue. This study aimed to evaluate the potential of Bacillus sp., FOBIO biopesticide, and green mustard (Brassica rapa L. var. parachinensis) as bioremediation agents for Cd-contaminated soil. The experiment was conducted using a Completely Randomized Design (CRD) with seven treatments and four replications. Observed parameters included Cd concentration in the soil before and after remediation, as well as the growth and physiological responses of mustard plants. The application of FOBIO biopesticide without plants resulted in the highest Cd reduction (4.57 mg/kg), while the combination of Bacillus sp. and mustard plants reduced Cd by 4.11 mg/kg, and mustard plants alone reduced Cd by 2.40 mg/kg, outperforming the control. FOBIO biopesticide application significantly enhanced leaf number, plant height, and fresh weight compared to other treatments. These findings indicate that Bacillus sp., FOBIO biopesticide, and green mustard have strong potential as bioremediation agents for Cd-contaminated soils.
Increasing the Growth and Yield of Okra (Abelmoschus esculentus) Plants with the Use of PGPR ( Plant Growth Promoting Rhizobacteria ) and Gibberellin Krisna Bayu Prasetiyo; Didik Utomo Pribadi; Felicitas Deru Dewanti; Sri Wiyatiningsih
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 8 No. 1 (2026): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 8 No. 1 January 2026
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v8i1.4844

Abstract

Okra (Abelmoschus esculentus) is a horticultural crop rich in minerals, antioxidants, polyphenols, and flavonoids. One of the limiting factors for okra production is inadequate nutrition. The use of Plant Growth-Promoting Rhizobacteria (PGPR) and gibberellins is a promising approach to enhance okra production. This study aimed to determine the optimal concentrations of PGPR and gibberellin combinations for improving the growth and yield of okra. The experiment was conducted from March to June 2025 at the Experimental Field of the Faculty of Agriculture, Veteran National Development University, East Java. A factorial experiment was designed using a Randomized Block Design (RBD) with two factors: PGPR concentration at four levels (0 ml/l, 10 ml/l, 15 ml/l, and 20 ml/l) and gibberellin concentration at four levels (0 ppm, 100 ppm, 150 ppm, and 200 ppm), resulting in 16 treatment combinations, each replicated three times. The interaction between PGPR and gibberellin treatments significantly affected plant height and the number of flowers. The 20 ml/l PGPR treatment had the most significant positive effect on the number of leaves between 28 and 49 days after planting and on fruit weight during the 1st, 3rd, and 5th harvests. The 200 ppm gibberellin treatment significantly accelerated flower emergence, with an average of 35.39 days to flower emergence. The combination of 20 ml/l PGPR and 200 ppm gibberellin was the most effective treatment for enhancing okra plant growth and yield.
The Effect of Shelf Life and the Effectiveness of Biopesticide Fobio in Controlling Stem Rot Disease in Vanilla Plants (Vanilla planifolia Andrews) Mutiara Alyzza Bilqis; Sri Wiyatiningsih; Tri Mujoko
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 8 No. 1 (2026): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 8 No. 1 January 2026
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v8i1.5017

Abstract

Stem rot disease caused by Fusarium oxysporum poses a significant challenge to vanilla (Vanilla planifoliaAndrews) cultivation. The use of microbial-based biopesticides, such as FOBIO, provides an environmentallyfriendly alternative to reduce reliance on chemical fungicides. This study aimed to evaluate the effects of FOBIObiopesticide storage age and concentration on disease intensity and vanilla plant growth (Vanilla planifoliaAndrews). The research was conducted from March to June 2025 in Dusun Tukum using a factorial completelyrandomized design with two factors: FOBIO storage age (6 months and 1 year) and concentrations (5, 7.5, and 10mL/L of water), with three replications totaling 105 plants. Data were analyzed using Analysis of Covariance(ANCOVA), followed by the Least Significant Difference (LSD) test at a 5% significance level. The resultsdemonstrated that the FOBIO application significantly reduced disease intensity compared to the control (p <0.05). The most effective treatment was FOBIO stored for 1 year at a concentration of 7.5 mL/L of water (FP23B),resulting in a final disease intensity of 6.365 compared to 38.255 in the control. FOBIO also increased leaf numberand stimulated plant growth during the vegetative phase, including accelerating flower development. The FOBIObiopesticide is an environmentally friendly alternative for controlling stem rot in vanilla and serves as a biologicalcontrol agent with potential to support sustainable agriculture.
Application of Biopriming Using Secondary Metabolites of Beauveria bassiana in Reducing the Intensity of Fusarium Wilt Disease in Chili Plants Nabila Naylofar; Sri Wiyatiningsih; Tri Mujoko; Vikayanti Vikayanti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.8385

Abstract

Background: Fusarium wilt, caused by Fusarium sp., is one of the main obstacles to chili pepper (Capsicum frutescens L.) production. Disease control using chemical pesticides and fungicides is considered effective in agricultural fields, but excessive use can result in environmental contamination and health risks, including cancer. This makes the application of biological control an effective strategy in overcoming Fusarium wilt disease in chili plants, especially in increasing plant productivity. This study aims to test the effectiveness of biopriming using Beauveria bassiana secondary metabolites in suppressing Fusarium wilt infection in chili seeds in vivo. Methodology: This study used a Completely Randomized Design (CRD). Secondary metabolites were applied to chili seeds at four concentration levels (15%, 25%, 35%, and 45%) as well as positive and negative controls. The treated seeds were planted in a medium inoculated with pathogens and observed for nine weeks. Disease intensity was recorded every seven days and analyzed using ANOVA followed by Tukey's Honestly Significant Difference (HSD) test at the 5% level. Findings:  The results of the variance test 49–63 days after sowing (DAS) showed significant differences between treatments; a further HSD test was conducted at a 5% level. The application of Beauveria bassiana secondary metabolites as a biopriming agent on chili seeds is considered to be ineffective. Contributions: This study provides the first in vivo evidence on the application of Beauveria bassiana secondary metabolites as a seed biopriming agent to suppress Fusarium wilt in chili plants, thereby advancing preventive biocontrol strategies and contributing to the development of environmentally sustainable plant disease management
Effectiveness of Fobio Biopesticide Concentration Against Stem Rot Disease in Vanilla Plants Winadya, Archamiyanis Putri; Wiyatiningsih, Sri; Mujoko, Tri
CROPSAVER Vol 9, No 1 (2026)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v9i1.67869

Abstract

Fusarium oxysporum is a soil-borne pathogen fungus that causes vanilla stem rot, which reduces vanilla productivity. This study aimed to determine the effectiveness of various concentrations of the biopesticide Fobio  in suppressing vanilla stem rot caused by Fusarium oxysporum. This study was conducted in vivo in Tukum Hamlet, Wonosalam District, Jombang Regency, East Java. This study used a Completely Randomized Design (CRD) with four treatments and five replications consisiting of : 5 ml/L (FA), 7.5 ml/L (FB), 10 ml/L (FC), and Control (K). The results showed that 7.5 ml/L was the best concentration, reducing disease intensity to 12.80%, with a biopesticide effectiveness of 51%.
EFFECTIVENESS TEST OF THE APPLICATION OF THE BIOPESTICIDE FOBIO IN CONTROL OF BROWN SPOT DISEASE (CURVULARIA SP.) ON RICE PLANTS (ORYZA SATIVA L.) IN RICE FIELDS IN PUTON VILLAGE, JOMBANG REGENCY, EAST JAVA Ustman Buchori; Sri Wiyatiningsih; Tri Mujoko
Multidiciplinary Output Research For Actual and International Issue (MORFAI) Vol. 6 No. 4 (2026): Multidiciplinary Output Research For Actual and International Issue
Publisher : RADJA PUBLIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Brown spot (Curvularia sp.) is a plant disease that poses a serious threat to cultivated crops such as rice. This study aims to test the effectiveness of the biopesticide FOBIO in suppressing the intensity of this disease and supporting rice plant growth. The study was conducted in Puton Village, Jombang, using a factorial randomized block design with a combination of FOBIO concentrations (0, 5, and 10 ml/L) and seed soaking times (10, 20, and 30 minutes). The results showed that FOBIO treatment, especially at a concentration of 10 ml/L for 30 minutes, effectively reduced disease intensity and increased plant growth rates. FOBIO has the potential to be an environmentally friendly alternative solution in an integrated pest control system that can be used in the long term.
Co-Authors Agustin, Indah Sari Dwi Alfia Rofika Sari Arika Purnawati Armadianty, Fany Risma Boleng, Maya Stolastika Deviyanti, Nur Qomariyah Dewanti, Felicitas Deru Diasi, Ananta Rizki Didik Utomo Pribadi Dinda Rizka Rahayu Dita Megasari Dwi Afrian Dwi Afrian Dwi Suci Rahayu Dykha Maretha Setyawan Eben, Philipus Wahyu Baskoro Eko Setiyo Wawan Elvira, Nolla Dwi Endang Tri Wahyu Prasetyawati Endang Triwahyu Prasetya Endang Triwahyu Prasetyawati Ersyaf, Ihza Muhammad F. Deru Dewanti Fajri Rizky Saptono Faradila Amir Farisa Fitri Wijayanti Fitriya Pebriana Gusdino Hasyidan Gusdino Hasyidan Harijani, Wiwik Sri Haykal, Arkan Hendra Wahyudi Ariyono Hendra Wahyudi Ariyono Herry Nirwanto Hery Nirwanto Ika Nur Fitriana Indah Anita Sari Krisna Bayu Prasetiyo Laila, Nita Nur Larrisa, Damasa Ines Lia Iswindari Mukaromah Maroeto ., Maroeto Maroeto Maroeto Moch Nur Yudha Moch. Arifin Mohamad Hipti Mutiara Alyzza Bilqis Nabila Alysia Multazam Nabila Naylofar Nauval Zakqy Nia Rulinggar Putri M Pangesti Nugrahani Penta SURYAMINARSIH Penta Suryaminarsih Pratama Wirya Atmaja Pribadi, Didik Utomo Rafi Dwi Nugraha Ramadhani Mahendra Kusuma Rani, Sasiska Ratna Arifiani Sukmaningrum Retno Sulistiyowati Riko Setya Wijaya Robithotul Ummah Rosyiidah Sholihin, Alyaa Arwaa Santoso, Wahyu Sarah Hikmah Marieska Sarah Hikmah Marieska Sari, Nadya Naurah Sentoso, Wahyu Septikahady, Hani Shafa, Yola Salsabila Shavira Dwi Livita Reza Sigit Dwi Nugroho Simanjuntak, William Fernando Sisko Budianto Siti Fatimatus Syahrok Surya Ari Widya Suryandika, Fadila Suwandi Tri Mujoko Tri MUJOKO Tri Wahyu Prasetyawati, Endang Ustman Buchori Vikayanti Vikayanti Wahyu Santoso Wahyu Santoso Wahyu Santoso Wanti Mindari Widyananda, Kanaya Syafina Wijaya, Riko Setya Wiludjeng Widajati Wiludjeng Widayati Wiludjeng Widiyati Winadya, Archamiyanis Putri Windriyanti, Wiwin Wiwik Sri Harijani Wiwik Sri Harijani Yenny WURYANDARI