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<![CDATA[Perbandingan Antibakteri dari Ekstrak Etanol dan Fraksi Ekstrak Etanol Tanaman Ceguk (Quisqualis indica L.) Terhadap Staphylococcus aureus dan Escherichia coli ]]>
<![CDATA[Valeri, Sepvan]]>;
<![CDATA[Soegianto, Lisa]]>;
<![CDATA[Wijaya, Sumi]]>
<![CDATA[ Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 2 No. 2 (2015) ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia ]]>
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DOI: 10.33508/jfst.v2i2.722
<![CDATA[Tanaman Ceguk merupakan tanaman yang berasal dari Myanmar dan Malaysia. Tanaman ini memiliki beberapa efek farmakologi antara lain immunomodulator, antihiperlipidemia, antipiretik, antioksidan dan antibakteri. Aktivitas antibakteri sebelumnya telah diteliti pada bagian bunga. Pada penelitian ini ekstrak etanol dan fraksi (n-heksana, etil asetat dan air) dari daun tanaman ceguk diuji aktivitas antibakterinya terhadap Staphylococcus aureus dan Escherichia coli. Fraksi aktif terpilih ditentukan dengan bioautografi kontak. Pengujian aktivitas antibakteri meliputi KHM (Kadar Hambat Minimum) dan KBM (Kadar Bunuh Minimum) dilakukan dengan metode mikrodilusi dan trifenil tetrazolium klorida 1% sebagai indikator visual. Golongan senyawa fraksi aktif ditentukan dengan uji kualitatif KLT dengan menggunakan penampak noda. Ekstrak dan fraksi n-heksana tanaman ini menghambat pertumbuhan pada bakteri Gram positif yaitu Staphylococcus aureus dengan nilai KHM 500 ppm pada fraksi n-heksana dan 1000 ppm untuk ekstrak. Ekstrak dan fraksi n-heksana tanaman ini tidak mampu membunuh Staphylococcus aureus dan Escherichia coli. Berdasarkan uji KLT, golongan senyawa fraksi aktif pada fraksi n-heksana yang diduga memiliki potensi sebagai antibakteri adalah golongan triterpenoid dengan harga Rf 0,63. Kata kunci : antibakteri, ceguk, fraksi, kadar hambat minimum, kadar bunuh minimum.]]>
<![CDATA[Karakterisasi dan Uji Aktivitas Antibakteri dari Fungi Endofit Ranting Tanaman Manggis (Garcinia mangostana L.) terhadap Escherichia coli dan Staphylococcus aureus ]]>
<![CDATA[Widjaja, Kevin]]>;
<![CDATA[Soegianto, Lisa]]>;
<![CDATA[Ervina, Martha]]>
<![CDATA[ Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 6 No. 1 (2019) ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia ]]>
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DOI: 10.33508/jfst.v6i1.2007
<![CDATA[Resistance is commonly caused by improper usage of antibiotics, so the treatment of the infection always needs a new and more potent compounds. Endophytic microbes are microbes that all or part of its life stay in the tissues of plant, which established a mutually beneficial relationship between them. In this research, isolation of endophytic fungi was conducted by sticking the sterilized twigs of mangosteen to the media Malt Extract Agar obtained three pure endophytic fungi colonies. The antibacterial activity assay with direct inoculation method at media Plate Count Agar which was inoculated with bacteria and showed that the antibacterial activity against Escherichia coli ATCC 8739 was one isolate (ER2) with inhibition average ratio 1.42 and against Staphylococcus aureus ATCC 6538 was three isolates (ER1, ER2, and ER3) with inhibition average ratio 1.66; 1.37; and 1.89. The fungus were observed characteristics of the macroscopic, microscopic and biochemical assay. ER1 and ER3 isolates are suspected as a Penicillium genus with specific characteristics such as the conidiophore, fialid, metulae, and conidiospore. ER2 isolate was suspected as a Rhizoctonia genus because there are nearly right-angled hyphae and monilioid cells.]]>
<![CDATA[Uji Aktivitas Antimikroba Ekstrak Etanol Daun Cayratia trifolia terhadap Staphylococcus aureus dan Candida albicans ]]>
<![CDATA[Sari, Rizki Sari Endah]]>;
<![CDATA[Soegianto, Lisa]]>;
<![CDATA[Hermanu, Liliek S,]]>
<![CDATA[ Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 5 No. 1 (2018) ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia ]]>
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DOI: 10.33508/jfst.v5i1.2052
<![CDATA[Cayratia trifolia is a wild plant that is easily found in the lowlands, tropical and subtropical regions. Cayratia trifolia is known to be efficacious as an antibacterial, antifungal, antiprotozoa, hypoglycemia, anticancer and diuretic. This study aims to determine whether the ethanol extract of Cayratia trifolia leaves has antimicrobial activity against Candida albicans and Staphylococcus aureus using well diffusion method; to determine the value of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) using microdilution method; and to determine the secondary metabolite groups which have antimicrobial activity against Staphylococcus aureus and Candida albicans. In this study bioautography method was carried out to determine the secondary metabolite group that can inhibit microbial growth. Ethanol extract of Cayratia trifolia (Galing-galing) leaves has no antimicrobial activity against Candida albicans. The MIC value of the ethanol extract of Cayratia trifolia (Galing-galing) leaves against Staphylococcus aureus is at a concentration of 125 ppm, while the MBCvalue is at a concentration of 250 ppm. The group of compounds that have antibacterial activity against Staphylococcus aureus is polyphenol, meanwhile flavonoid suspected has activity against Candida albicans.]]>
<![CDATA[Studi pendahuluan: Korelasi Aktivitas Antikolesterol dengan Aktivitas Antioksidan Ekstrak Etanol Daun Salam (Syzygium polyanthum) ]]>
<![CDATA[Wijaya, Sumi]]>;
<![CDATA[Yonas, Stefani Maureen Kasih]]>;
<![CDATA[Hartanti, Lanny]]>;
<![CDATA[Setiawan, Henry K.]]>;
<![CDATA[Soegianto, Lisa]]>
<![CDATA[ Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 5 No. 2 (2018) ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia ]]>
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DOI: 10.33508/jfst.v5i2.2143
<![CDATA[Bay leaf leaves (Syzygium polyanthum) are known to have anti-cholesterol activity, with the mechanism inhibited HMG-CoA Reductase enzyme. The purpose of this study was to determine the correlation between anticholesterol activity of bay leaf ethanol extract obtained by percolation method and its antioxidant activity. The parameter used to measure anticolesterol activity is the IC50 value. Antioxidant activity was evaluated by DPPH and Frap methods. The results showed that the ethanolic extract of bay leaves had an IC50 value of 49.50 ± 0.70 ppm. Ethanol extract of bay leaf showed antioxidant activity with IC50 value of 888.0835 ppm and equality of values with 1 mM FeSO4 of 295 ppm. Based on the results of the analysis, the anticholesterol activity of Bay leaf ethanol extract in inhibiting the HMG-CoA Reductase enzyme did not have a linear correlation with its antioxidant activity.]]>
<![CDATA[Isolasi dan Karakterisasi Fungi Endofit dari Daun Tanaman Bintaro (Cerbera odollam) yang Berpotensi sebagai Antibakteri ]]>
<![CDATA[Wonowijaya, Suwandi]]>;
<![CDATA[Soegianto, Lisa]]>
<![CDATA[ Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 5 No. 2 (2018) ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia ]]>
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DOI: 10.33508/jfst.v5i2.2144
<![CDATA[The need of antibiotics is increasing, spurred to find compounds that have antimicrobial activity from natural ingredients. One way to obtain antimicrobial compounds is through endophytic fungi. This study aimed to isolate and characterize endophytic fungi obtained from the leaves of the plant Bintaro (Cerbera odollam) that potential as an antibacterial against Staphylococcus aureus and Escherichia coli. Leaf surface was sterilized by soaking in 70% alcohol for 2 minutes, 5.3% sodium hypochlorite for 5 minutes, 70% alcohol for 1 minute, rinsed with sterile distilled water and dried using sterile wipes. Bintaro leaf that have been sterilized was puton Potato Dextrose Agar (PDA) and then incubated at room temperature for 7-14 days. The growing fungi was inoculated in Potato Dextrose Yeast (PDY) for 7-14 days at room temperature based on the macroscopic difference. Endophytic fungi that have been purein macroscopic way was tested antibacterial activity on Staphylococcus aureus and Escherichia coli and continued to macroscopic and microscopic observation. Obtained three types of endophytic fungi were isolated from bintaro leaves (Cerbera odollam) with the code EB 1, EB 2 and EB 3 that suspected from genus Fusarium, Geotrichum and Aspergillus. The antibacterial activity test show that results of three endophytic fungi obtained has no antibacterial activity against Staphylococcus aureus and Escherichia coli.]]>
<![CDATA[Potensi Antibakteri dan Antibiofilm Ekstrak Etanol Bunga Bintaro (Cerbera odollam) terhadap Staphylococcus aureus ATCC 6538 ]]>
<![CDATA[Lestari, Dwi Rahma Suci]]>;
<![CDATA[Soegianto, Lisa]]>;
<![CDATA[Hermanu, Liliek S.]]>
<![CDATA[ Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 4 No. 1 (2017) ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia ]]>
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DOI: 10.33508/jfst.v4i1.2176
<![CDATA[Cerbera odollam was one of the plants that can inhibit the growth of bacteria that cause infectious diseases. The misuse of antibiotic in the treatment of infection frequent caused bacterial resistance. One of the forms of antibiotic resistance was the capability of microorganism forming biofilm. This study was conducted to determine the antibacterial and anti-biofilm activity of ethanolic extract from Cerbera odollam flower against Staphylococcus aureus. The ethanolic extract obtained by maceration method using 96% ethanol as a solvent. The ethanol extract of Suicide tree (10, 20 and 30%) were tested for antibacterial activity against Staphylococcus aureus using well diffusion method and anti-biofilm activity using microdilution method. Based on the results, ethanol extract of flowers Cerbera odollam inhibit the growth of Staphylococcus aureus with inhibition zone of 21.66±0.73 mm (10%), 25.66±1.50 mm (20%) and 27.10±1.06 mm (30%). The ethanol extract of Suicide tree flower inhibit biofilm formation 98.29% at the concentration of 3.75%. The bio autography results showed that alkaloid and tannin responsible as bioactive compounds from the ethanol extract Suicide tree flower.]]>
<![CDATA[Uji Potensi Antibakteri dan Antibiofilm Minyak Atsiri Umbi Teki (Cyperus rotundus L.) terhadap Staphylococcus aureus ATCC 6538 ]]>
<![CDATA[Uthari, Ni Made]]>;
<![CDATA[Soegianto, Lisa]]>;
<![CDATA[Hermanu, Liliek S.]]>
<![CDATA[ Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 4 No. 2 (2017) ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia ]]>
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DOI: 10.33508/jfst.v4i2.2183
<![CDATA[Infectious disease is a disease that is caused by an invasion of pathogenic microorganisms in the body. Biofilm is a group of microorganisms that are able to differentiate and proliferate in the complex way as well as being able to communicate between cells through a matrix of polysaccharides. In chronic infection, biofilm has an important role to maintain the bacteria which can cause resistance to antibiotics. Teki (Cyperus rotundus L.) is one of the plants that has many benefits for different treatment. The aim of this study was to determine the antibacterial activity and inhibition of biofilm activity of essential oil of Cyperus rotundus L. tubers against Staphylococcus aureus. Essential oil was extracted by distillation using Stahl with water as the solvent and the yield of essential oil obtained was 0.22%. Determination of antibacterial activity and antibiofilm activity were carried out by microdilution method using microplate 96 U-PVC bottom wells against Staphylococcus aureus which is equivalent to the solution standard of ½ Mc Farland I. The results of antibacterial activity tests showed that MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) values were 0.05% (v/v) and 12.5% (v/v). Antibiofilm activity test showed that the essential oil of Cyperus rotundus L. tubers is able to inhibit 89.01% of the biofilm formation of Staphylococcus aureus on the concentration of 0.09% (v/v).]]>
<![CDATA[Uji Aktivitas Antibakteri dan Antibiofilm Fraksi Biji Kelengkeng (Euphoria longan Lour. Steud.) terhadap Staphylococcus aureus ATCC 6538 ]]>
<![CDATA[Prasety, Oda Shantina]]>;
<![CDATA[Soegianto, Lisa]]>;
<![CDATA[Wijaya, Sumi]]>
<![CDATA[ Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 6 No. 2 (2019) ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia ]]>
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DOI: 10.33508/jfst.v6i2.2235
<![CDATA[Longan (Euphoria longan Lour. Steud.) has an antibacterial potential. Longan seed is one part of Longan that has antibacterial activity. This study was conducted to determine the antibacterial and antibiofilm activity of longan seed fraction (Euphoria longan Lour. Steud.) against Staphylococcus aureus ATCC 6538. In this study, the extract was obtained from maceration using 96% ethanol and it was fractionated by liquid-liquid solvent method using nhexane, ethyl acetate, and water. Each fraction prepared at concentration 100000 ppm, and then the antibacterial activity on Staphylococcus aureus was determined by well diffusion method, antibiofilm activity using microdilution method, and phytochemical screening to determine the metabolite compounds that have antibacterial and antibiofilm activity using thin layer chromatography. The results showed that the diameter of growth inhibition in ethyl acetate fraction with a concentration of 100000 ppm was 14.59 mm ± 0.01, n-hexane fraction at 7.15 mm ± 0.63 and water fraction of 12.52 mm ± 0.40. Antibiofilm test results showed that the percentage of growth inhibition of biofilm on the fraction of ethyl acetate with a concentration of 100000 ppm was 99.08%. Phytochemical screening using thin layer chromatography carried out on the fraction of ethyl acetate and showed that the fraction contains tannin/polyphenols, triterpenoid saponins and flavonoids.]]>
<![CDATA[Uji Aktivitas Antibakteri dan Antikuorum Sensing Fraksi dari Ekstrak Etanol Daun Salam (Eugenia polyantha Wight.) ]]>
<![CDATA[Ardani, Yonas Bianityo]]>;
<![CDATA[Soegianto, Lisa]]>;
<![CDATA[Wijaya, Sumi]]>
<![CDATA[ Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 1 No. 1 (2013) ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia ]]>
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DOI: 10.33508/jfst.v1i1.2343
<![CDATA[Eugenia polyantha is being used as empirical plant for antiseptic, anthelmintic, anesthetic, keratolytic and also has properties protein in bacterial cells. The antibacterial activity of extract of Eugenia polyantha has been proved in previous study, therefore this research was conducted to know the antibacterial and antiquorum sensing from fractions of ethanolic extract of Eugenia polyantha. The extraction was prepared by maceration and fraction was prepared by liquid-liquid fractionation method with n-hexane (non polar), ethyl acetate (semipolar) and water (polar). Bioautography method with thin layer chromatography and chloroform as mobile phase was conducted to determine the active fraction. The diameter of inhibition zone was measured or determined by diffusion method, while concentration of minimum inhibition (MIC) and bactericidal concentration (MBC) were determined using broth and agar dilution methods. Antiquorum sensing activity for P. aeruginosa was carried on, using pigment survey and motility method~ The antibacterial activities showed that ethyl acetate fraction 2596 gave diameter of growth inhibition zone greater than others, 1:1.48 mm against S. aureus and 9.03 mm against P. aeruginosa. The MIC of ethyl acetate fraction gave value of :1.56% against S. aureus and 12.5% against P. Aeruginosa, meanwhile the MBC was 3.1396 against S. aureus and more than 12.5% against P. aeruginosa. The result showed that ethyl acetate fraction has potential antiquorum sensing activity on concentration of 2.5 %. The active fraction of ethanolic extract of Eugenia polyantha which has antibacterial and antiquorum sensing activity was ethyl acetate fraction.]]>
<![CDATA[Skrining Senyawa Antibakteri dari Minyak Atsiri Rimpang Temu Kunci (Boesenbergia pandurata) terhadap Staphylococcus aureus dengan Metode Bioautografi Kontak ]]>
<![CDATA[Christiana, Indah]]>;
<![CDATA[Soegianto, Lisa]]>
<![CDATA[ Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 7 No. 1 (2020): Februari ]]>
Publisher : <![CDATA[Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia ]]>
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DOI: 10.33508/jfst.v7i1.2391
<![CDATA[Fingerroot are generally used as a remedy for rheumatism, gastroenteritis, mucositis, diuretic, malaria, bowel disorders, flatulence, skin diseases, diarrhea, mouth sores, and intestinal worms. Essential oils found in Fingerroot are generally used as antibacterial. The purpose of this study is to determine the antibacterial activity of Fingerroot essential oils against Staphylococcus aureus and to determine the antibacterial compound of Fingerroot essential oils (Boesenbergia pandurata) using contact biautography methods. Fingerroot essential oil was obtained by the Stahl distillation method. Determination of the class of efficacious compounds was carried out by contact bioautography method and anisaldehyde-sulfuric acid and vanillin sulfate were used as spray reagent. The Fingerroot essential oil yields 0.38% and monoterpenes was determined as an antibacterial agent against Staphylococcus aureus.]]>
