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PENGARUH PEMASTEURAN TANAH TUNGGAL ATAU DIGABUNG AGENSIA HAYATI TERHADAP PENYAKIT BUSUK HATI DI PEMBIBITAN PISANG Haryono, Joko; Prihatiningsih, Nur; Wardhana, Rahman A.; Soesanto, Loekas
Agrin Vol 12, No 2 (2008): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2008.12.2.91

Abstract

Tujuan penelitian ini adalah mengetahui pengaruh pemasteuran medium digabung dengan agensia hayatidan agensia hayati yang paling efektif terhadap penyakit busuk hati. Penelitian dilakukan di PT NusantaraTropical fruit, Lampung Timur dengan rancangan Petak Terbagi dan diulang tiga kali. Petak utama adalahmedium dipasteur atau tidak. Anak-petak adalah control dengan air steril, Trichoderma harzianum isolat jahedan pisang, Pseudomonas fluorescens P60, dan Fusarium equiseti diisolasi dari akar pisang. Peubah yangdiamati adalah masa inkubasi, intensitas penyakit, populasi konidium Fusarium akhir, akar berpotensi terinfeksi,tinggi tanaman, jumlah daun, jumlah akar primer dan sekunder, panjang akar, dan berat akar. Hasil penelitianmenunjukkan bahwa perlakuan gabungan memberikan hasil positif khususnya menurunkan intensitas penyakitsampai 43,57%. Agensia hayati T. harzianum isolat pisang dan P. fluroescens P60 yang digabung denganpemasteuran medium dapat menekan intensitas penyakit masing-masing sebesar 63,08 dan 59,75%. Agensiahayati yang paling efektif adalah T. harzianum isolat pisang karena menekan kepadatan Fusarium, meningkatkantinggi tanaman, dan meningkatkan berat akar masing-masing sebesar 41,12, 39,00, dan 98,86%.Kata kunci: busuk hati, bibit pisang, pemasteuran, agensia hayati ABSTRACTThe objectives of this research were to know the effect of pasteurized media combined with biologicalagents and the most effective biological agent on heart rot disease. The research was carried out at PT NusantaraTropical Fruit, East Lampung designed by Split Plot Design and repeated three times. The main plot waspasteurized and unpasteurized media. The subplot was control with sterile water or fungicide, Trichodermaharzianum isolated from ginger or banana, Pseudomonas fluorescens P60, and Fusarium equiseti isolated frombanana root. Variables observed were incubation period, disease intensity, late Fusarium conidial population,potentially infected root, crop height, leave numbers, primary or secondary root numbers, root length, and rootweight. Result of the research indicated that the combination treatments gave positively result specially to reducedisease intensity of 43.57%. Biological agents of T. harzianum banana isolate and P. fluorescens P60 combinedwith the pasteurisation could suppress disease intensity of 63.08 and 59.57%, respectively. The most effectivebiocontrol agent was T. harzianum banana isolate because of suppressing Fusarium density, increasing plantheight, and increasing root weight as 41.12, 39.00, and 98.86%, respectively.Key words: heart rot, banana seedlings, pasteurization, biological agents 
ABILITY TEST OF SEVERAL ANTAGONISTS TO CONTROL POTATO BACTERIAL WILT IN THE FIELD Soesanto, Loekas; Manan, Abdul; Wachjadi, Muljo; Mugiastuti, Endang
AGRIVITA Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.227

Abstract

The research objective was to know ability of antagonistic microbes to control bacterial wilt on potato in the field. This research was carried out at Serang Village, Karangreja Subdistrict, Purbalingga Regency from June up to August 2012. The antagonist, originally isolated from potato field, was Bacillus sp. B2 and B4, and Pseudomonas sp. P19 and P20. Based on the research result, Pseudomonas P19 could control the disease on potato with delaying incubation period of 78.95%, suppressing disease intensity of 51.57%, decreasing final pathogenic population of 99.74%, and inducing plant resistance with increasing saponin, tannin, and glycoside content. However, the antagonist could not increase growth and yield of potato.  Keywords: antagonistic microbes, bacterial wilt, potato
Pendampingan Demplot Teknik Budidaya Bawang Merah pada Musim Hujan Secara Ramah Lingkungan Manan, Abdul; Soesanto, Loekas; Mugiastuti, Endang; Rahayuniati, Ruth Feti
Jurnal SOLMA Vol. 13 No. 1 (2024)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v13i1.13551

Abstract

Background: Shallot cultivation in the rainy season faces the constraints of more severe plant disease problems. Utilization of mixed biopestisda Trichoderma harzianum, Bacillus sp. B8 and Pseudomonas flourescens P8 as well as proper cultivation techniques have not been adopted by farmers. It is necessary to assist farmers in implementing the technology The objectives of the activity are: assisting farmers in implementing the shallot cultivation technology package appropriately, increasing the quantity and quality of crop production, and increasing farmers' income through increasing farm efficiency. Method: The farmers participating in the activity are members of the farmer group "Lestari" Tambak Sogra Village, Sumbang District, Banyumas Regency. Technology transfer method through demplot assistance. The biopesticide technology package and environmental manipulation are compared to the conventional package of synthetic chemical pesticides. Data on the growth and yield of technological plot crops compared to conventional plots. The large number of farmers who can independently apply the technology is observed.  Results: mentoring activities showed that farmer group members were able to independently implement technology packages. The use of biopesticides and proper cultivation techniques can produce growth and yield equivalent to the use of synthetic chemical pesticides. Conclusion: 80% partner farmers can apply technology packages, the use of biopesticides is able to substitute the use of synthetic chemical pesticides, technology packages are able to increase 5.9% of shallot bulbs and reduce 75% production costs.
Pendampingan Pembuatan Pestisida Ramah Lingkungan dan Aplikasinya di Training Center Desa Sumberjati, Kecamatan Jatirejo, Kabupaten Mojokerto Soesanto, Loekas; Sastyawan, Murti Wisnu Ragil
Alamtana: Jurnal Pengabdian Masyarakat UNW Mataram Vol 7 No 1 (2026): Edisi Mei 2026
Publisher : LPPM UNIVERSITAS NAHDLATUL WATHAN MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/jaltn.v7i1.2787

Abstract

Abstract: Permasalahan dalam budidaya tanaman pertanian terutama adalah adanya serangan hama dan penyakit tanaman. Petani selalu menggunakan pestisida kimia untuk mengendalikannya, namun, penggunaan yang tidak bijaksana dapat menyebabkan dampak negatif. Alternatif pengendalian yang aman dan ramah lingkungan diperlukan, salah satunya menggunakan pestisida organik berbasis metabolit sekunder mikroba antagonis. Pengetahuan akan pembuatan dan aplikasi pestisida organik masih belum dikenal oleh petani muda dari daerah tertinggal, sehingga perlu dilakukan pelatihan pembuatan pestisida organik dan cara aplikasinya. Pelatihan dilakukan selama satu hari dengan pendampingan selama tiga bulan, di training center Desa Sumberjati, Kecamatan Jatiroto, kabupaten Mojokerto, yang diikuti 12 petani muda dari Nusa Tenggara Timur, Pulau Nias, dan Kalimantan Barat. Pelatihan diberikan dalam bentuk pemberian materi terkait pesitida organik dan aplikasinya, pengenalan mikroba antagonis, pembuatan pestisida organik secara praktis, pembuatan alat infus sederhana, dan cara aplikasi pestisida organik ke tanaman buah. Hasil pelatihan menunjukkan adanya peningkatan pengetahuan dan ketrampilan peserta terkait materi yang diberikan. Pemahaman akan hama dan penyakit tanaman, dampak negatif pestisida kimia, peran penting pestisida organik, pembuatan pestisida organik secara mudah dan praktis, dan aplikasinya pada tanaman budidaya dapat diterima dan dipraktikkan. Pelatihan pembuatan alat infus sederhana dapat dipraktikkan dengan mudah. Pengetahuan dan ketrampilan peserta dapat sebagai bekal untuk dipraktikkan dan ditularkan ke petani lainnya ketika peserta Kembali ke daerah masing-masing.
Article Review: The Role of Cyrtorhinus lividipennis in Rice Planthoponus Control and Food Security Cahyani, Maulin Eka; Risna, Rafa Aida Ainur; Primadani, Aura Helga; Nugraha, Sultan; Soesanto, Loekas
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11884

Abstract

Rice (Oryza sativa L.) is the primary food source for the majority of the world’s population, particularly in Asia. Leafhoppers are among the primary organisms that damage farmers’ crops, especially rice. Improper control measures, particularly the use of insecticides, can lead to negative impacts such as pest resistance and resurgence; furthermore, the use of insecticides can reduce natural enemy populations. The use of natural predators such as Cyrtorhinus lividipennis serves as an alternative in biological control. This article aims to examine the role of C. lividipennis in controlling brown planthopper populations in rice crops and its impact on food security. The method used was a literature review analyzing various research findings related to brown planthopper population dynamics, predator density, predation rates, and their impact on rice productivity. The results of the study indicate that the presence of C. lividipennis can significantly suppress brown planthopper populations through predation, with predation rates of 50–80%. Predator density is negatively correlated with brown planthopper populations. The impact of this control is also evident in reduced crop damage and increased rice growth and productivity. Thus, C. lividipennis has the potential to serve as a biological control agent to support food security.  
INVENTARISASI JAMUR PATOGEN TULAR-BENIH PADA LIMA VARIETAS PADI Sobianti, Sifa; Soesanto, Loekas; Hadi, Suciati
Agro Bali : Agricultural Journal Vol 3, No 1 (2020)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (793.301 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui (1) beragam spesies jamur patogen tular-benih pada lima varietas padi, dan (2) persentase daya kecambah lima varietas padi. Penelitian dilaksanakan di Laboratorium Karantina Pertanian Kelas I Semarang, dari bulan Maret sampai dengan Mei 2019. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 5 ulangan, perlakuan terdiri atas Varietas Inpago Unsoed 1, Inpago Unsoed Parimas, IR64, Ciherang, dan Situ Bagendit. Isolasi dan identifikasi jamur patogen tular-benih dilakukan pada blotter test dan medium PDA, dengan pengamatan di bawah mikroskop stereo dan kompon, kemudian dibandingkan dengan pustaka. Variabel yang diamati yaitu morfologi koloni patogen, morfologi patogen, dan daya kecambah. Hasil penelitian menunjukkan bahwa (1) pada semua varietas benih padi terdapat jamur patogen tular-benih, tetapi tidak semua jamur tersebut dijumpai pada setiap varietas. Jenis jamur patogen tular-benih yang dijumpai, yaitu Alternaria padwickii Ganguly, Aspergillus flavus Link, Aspergillus niger van Tieghem, Curvularia lunata (Wakker) Boedjin, Curvularia pallescens Boedjin, Drechslera oryzae Breda de Haan, Fusarium semitectum Berk. & Rav. [W&R, G,B,J], Rhizoctonia solani J. G. Kuhn, Rhizopus oryzae Went & Prins. Geerl., dan Tilletia barclayana Bref., dan (2) uji daya kecambah menunjukkan bahwa masing-masing varietas benih padi memilki persentase daya kecambah benih yang berbeda. Daya kecambah benih berturut-turut pada varietas IR64, Ciherang, Inpago Unsoed 1, Inpago Unsoed Parimas, dan Situ Bagendit sebesar 85,75, 81,75, 80,25, 76,5, dan 70%.DOI: 10.37637/ab.v3i1.416
UJI EMPAT ISOLAT Trichoderma harzianum PADA PENGOMPOSAN KOTORAN SAPI DAN AYAM DAN PENGARUHNYA TERHADAP PERTUMBUHAN MENTIMUN IN PLANTA Oktafiyanto, Muhammad Firdaus; Soesanto, Loekas; Mugiastuti, Endang; Rahayuniati, Ruth Feti; Tamad, Tamad
Agro Bali : Agricultural Journal Vol 3, No 1 (2020)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.796 KB)

Abstract

Penelitian bertujuan: 1) mengetahui pengaruh empat isolat Trichoderma harzianum dalam pengomposan kotoran (ayam dan sapi), 2) interaksi T. harzianum dan kotoran terhadap pertumbuhan, dan 3) kompos terbaik untuk tanaman mentimun. Rancangan Acak Kelompok digunakan dengan 10 perlakuan dan 3 ulangan. Perlakuan terdiri atas kotoran ayam dan sapi, T. harzianum. T10, T213, T14, dan T16.. Variabel yang diamati pH, C/N rasio, suhu, warna kompos, kepadatan dan kegigasan konidium, komponen pertumbuhan, analis jaringan tanaman, dan serapan N tanaman. T. harzianum T10 dan T14 mampu menurunkan C/N rasio pada kotoran sapi. T. harzianum terbaik adalah T14 pada kotoran sapi yang memiliki nilai tertinggi pada panjang tanaman sebesar 76 cm, panjang akar 22 cm, dan berat basah tajuk 14,96 g. T. harzianum T10 pada kotoran sapi memiiki nilai tertinggi pada berat tanaman segar 17,86 g, bobot basah akar 3,04 g, dan jumlah daun 8,88 helai.DOI: 10.37637/ab.v3i1.424
Potential of Beauveria bassiana Controlling Nilaparvata lugens for Food Security: A Review Article Fathurohman, Fijar Muhamad; Parsa, Raisa Shakila; Lestari, Indah; Falihah, Farica; Soesanto, Loekas
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11885

Abstract

The Brown Planthopper (Nilaparvata lugens Stål) is a strategic pest threatening the stability of national rice production. Farmers' dependence on synthetic insecticides has triggered pest resistance and environmental degradation, making the utilization of biological agents such as the entomopathogenic fungus Beauveria bassiana an urgent alternative solution. This study aims to comprehensively analyze the potential, mechanisms, and effectiveness of B. bassiana in controlling N. lugens. The method used is a Systematic Literature Review (SLR) by examining experimental articles from accredited national journals and reputable international journals published between 2018 and 2025. Results of data synthesis indicate that B. bassiana has a very high level of pathogenicity (nymph mortality >78%), especially when using local isolates cultured on corn media. However, its effectiveness is highly influenced by abiotic factors, where conidial viability is optimal at 25°C and drops sharply at extreme temperatures (34°C). Recent findings prove that B. bassiana can colonize as an endophyte within rice tissues, suppressing planthopper fecundity while simultaneously inducing systemic plant resistance. Additionally, this fungus is proven compatible and synergistic when combined with sublethal insecticide doses. It is concluded that B. bassiana is effective as both a contact biopesticide and an internal protection agent within Integrated Pest Management.  
Potential of Various Rice-Washing Water as a Source of Manufacturing Secondary Metabolites of Trichoderma harzianum T10 to Control Cucumber Crown and Root Rot Soesanto, Loekas; Dapawole, Michelia Alba; Sastyawan, Murti Wisnu Ragil; Mugiastuti, Endang; Manan, Abdul; Istiqomah, Dina
Agrotechnology Research Journal Vol 8, No 2 (2024): Agrotechnology Research Jurnal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrotechresj.v8i2.92490

Abstract

Cucumber is one of the most widely consumed vegetable crops globally. Rice-washing water has not been previously explored as a potential source for producing secondary metabolites from antagonistic fungi. Phytophthora species frequently affect cucumber seedlings, leading to crown and root rot. This research aimed to assess the efficacy of washing water from various rice types on the conidial density of Trichoderma harzianum T10 and its impact on crown and root rot in cucumber seedlings and overall plant growth. We conducted four treatments with rice washing water in vitro under a completely randomized design, comprising six replicates. Five treatments were evaluated in planta under a randomized block design with five replicates. The observed variables included conidial density, incubation period, disease incidence, disease progression expressed as the Area Under the Disease Progress Curve (AUDPC), plant height, fresh weight, and root length. The results indicated that washing water from glutinous rice provided the optimal medium for T. harzianum T10, yielding a conidial density of 10.3 × 10-6 conidia mL-1, representing a 66.02% increase compared to washing water from white rice. The crude secondary metabolites produced by T. harzianum T10 in glutinous rice washing water significantly extended the incubation period and reduced disease incidence and AUDPC values by 40.34, 62.07, and 69.41%, respectively, compared to the control. Furthermore, the secondary metabolites from T. harzianum T10 in glutinous rice washing water enhanced plant height, fresh weight, and root length by 91.81, 92.42, and 95.21%, respectively, compared to the control.
Iron Contaminated Soils Remediation Using Secondary Metabolites of Trichoderma harzianum T10 and Its Effect on Spinach Growth Soesanto, Loekas; Sastyawan, Murti Wisnu Ragil; Mugiastuti, Endang; Tamad, Tamad
Agrotechnology Research Journal Vol 7, No 2 (2023): Agrotechnology Research Jurnal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrotechresj.v7i2.79946

Abstract

Iron (Fe) is an essential metal whose presence in excess can pollute the environment, cause toxic effects on plants, and degrade soil quality. Efforts have been made to overcome this by remediation using secondary metabolites of Trichoderma harzianum T10. This study aimed to determine the potency and appropriate concentration of T. harzianum T10 secondary metabolites as a remediator for Fe-contaminated soil and its effect on the growth and yield of spinach grown on remediated soil. The research was conducted at the Screen House, Soil Laboratory, and Plant Protection Laboratory, Faculty of Agriculture, Universitas Jenderal Soedirman for four months. A randomized block design was used with treatment consisting of control and secondary metabolites application of T. harzianum T10 concentrations of 25, 50, 75, and 100%, repeated five times. The secondary metabolites were applied in the afternoon by pouring it on the soil in polybags and letting it stand for 10 days in tightly closed conditions. The variables observed were Fe content in the soil, plant height, number of leaves, shoot fresh weight, fresh root weight, and root length of spinach plants. The results showed that the secondary metabolites of T. harzianum T10 have the potential to remediate iron-congested soil. The content of Fe in the soil is 823 ppm. The appropriate concentration of T. harzianum T10 secondary metabolites as a remediator for Fe-contaminated soil is 50%, which can reduce the content of Fe (iron) by 46% compared to controls. The application of T. harzianum T10 secondary metabolites has not affected the growth and yield of spinach, which was grown on remediated soil, although there is a tendency to be better.