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Journal : Proceedings of the International Conference on Applied Science and Health

EFFECT OF TREE MARIGOLD (Tithonia diversifolia (HEMSL.) A. GRAY) LEAVES INFUSION AND ETHANOL EXTRACT ON BLOOD GLUCOSE LEVEL AND LIVER HISTOPATHOLOGY OF STREPTOZOTOCIN-INDUCED MALE WISTAR RATS Maulidiany, Rachma Ayu; Suhaeni, Eni; Sulistiyana, Catur Setiya; Sari, Ariestya Indah Permata
Proceedings of the International Conference on Applied Science and Health No 3 (2018)
Publisher : Proceedings of the International Conference on Applied Science and Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.222 KB)

Abstract

Background: Common Indonesians often use alternative therapy for Diabetes Mellitus (DM) due to its high prevalence. Tree marigold (Tithonia difersivolia (Hemsl.) A. Gray) leaves show its positive effect in decreasing blood glucose level. Thus, it is important to analyze the negative effect of consuming the proposing alternative including its toxicity level to vital organs. This study aims to observe the blood glucose level and liver histopathology in streptozotocin-induced diabetic male rats treated by infusion and ethanol extract of the tree marigold leaves. Methods: Pretest and posttest with control group design experiment was conducted to analyze effect on blood glucose level and posttest only with control group design was for determining liver histopathology. Twenty-four male white rats (Wistar strain) were randomly divided into 4 groups: normal control group (KN), positive control group (K+) treated with 0.45mg/kg of glibenclamid, treatment group 1 (P1) treated by 100 mg/kg of tree marigold leaves ethanol extract, and treatment group 2 (P2) treated by 1373 mg/kg of tree marigold leaves infusion. The rats at the groups of K+, P1, and P2 were induced by streptozotocin to reach hyperglycemic state. Comparison of blood glucose level effect between infusion and ethanol extract was analyzed by One-Way ANOVA continued by post-hoc test, while comparison of liver histopathology effect was determined by Kruskal-Wallis continued by Mann-Whitney test. Results: Significant differences of blood glucose level decrease were found in all groups. Group P2 significantly decreased blood glucose level more than P1 (p=0.000) as much as 124.97 mg/dl and 101.46 mg/dl respectively. Significant differences of liver histopathology were obtained between KN vs K(+), KN vs P2, K(+) vs P2, and P1 vs P2. Group P2 showed more severe of liver histopathology changes than P1 (p=0.025). Conclusion: Tree marigold leaves extract is more effective in lowering blood glucose level and has less toxic effect to liver histopathology than its infusion. 
CALCULATING COLONY OF Candida spp. AMONG CHILDREN WITH INTELLECTUAL DISABILITY IN CIREBON Oktaviani, Dhini; Amanah, Amanah; Suhaeni, Eni; Primanagara, Risnandya
Proceedings of the International Conference on Applied Science and Health No 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.164 KB)

Abstract

Background: Children and adults with Intellectual Disability and Autism Spectrum Disorders (ASD) have decreased intestinal permeability and enzyme activity, causing gastrointestinal (GI) problems that are more frequent and more severe than in children from the general population. The immune system which is part of the intestinal barrier in children with Intellectual Disability (ID) experiences various disorders and becomes more susceptible to fungal invasion, such as Candida spp. This present study aims to calculate the colony of Candida spp. in the digestive tract of Children with Intellectual Disability (ID) in the city of Cirebon.Method: This is an observational study with cross-sectional design using purposive sampling. The study was conducted in November 2018-February 2019. The sample (ID) group consists of 31 individuals from Special Needs School and 60 individuals from National elementary school (non-ID) in the city of Cirebon. The inclusion criteria were children ages 6-12 years and the exclusion criteria were children under antifungal treatment and children under cytotoxic or immunosuppressive drugs.  The fecal samples from the ID group and non-ID were examined at the Laboratory of the Faculty of Medicine, Universitas Swadaya Gunung Jati Cirebon, Indonesia using Digital Colony counter. Collected data were stored and analyzed using computer aided Statistical program. Mann-Whitney analysis of variance techniques were used to test the hypotheses. Results: The results showed that there are significant differences in the colonies of Candida spp. between the ID group and non-ID group. Median colony of Candida spp. in the ID group is 39.00 with mean colony of 126.90 while the median colony of non-ID group is 3.00 with mean colony of 40.93 (p<0.001).Conclusion: There are significant differences between the Candida spp. colonies in the ID group with the non-ID.
EFFECT OF PHYSICAL ACTIVITY AND FAST FOOD CONSUMPTION FREQUENCY ON OVERWEIGHT LEVEL AMONG HIGH SCHOOL ADOLESCENT Harlianti, Lita; Pratiwi, Witri; Nauphar, Donny; Suhaeni, Eni
Proceedings of the International Conference on Applied Science and Health No 3 (2018)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.214 KB)

Abstract

Background: Obesity was believed to be one of the risk factors of degenerative diseases, such as diabetes mellitus, hypertension, and coronary heart disease. Obesity occurred gradually that started with overweight. Risk factors for overweight are lack of physical activity, and consumption of high caloric fatty food which may cause accumulation of fats inside the body. This research aims to examine the effect of physical activity and fast food consumption frequency on overweight level among high school adolescent in Cirebon. Methods: It was a cross-sectional study conducted among 262 high school students aged 15-17 years using stratified random sampling. Results: The result based on Spearman correlation test showed negative correlation between physical activity and overweight level with p= 0,001 (p< 0,05) and correlation coefficient of -0,627. There was positive correlation between consumption fast food frequency with overweight with p=0,037 (p=0,05) and correlation coefficient of 0,423. Conclusions: Physical activity and fast food consumption frequency have effects on overweight level in high school adolescent in Cirebon. 
EFFECT OF TREE MARIGOLD (Tithonia diversifolia (HEMSL.) A. GRAY) LEAVES INFUSION AND ETHANOL EXTRACT ON BLOOD GLUCOSE LEVEL AND LIVER HISTOPATHOLOGY OF STREPTOZOTOCIN-INDUCED MALE WISTAR RATS Rachma Ayu Maulidiany; Eni Suhaeni; Catur Setiya Sulistiyana; Ariestya Indah Permata Sari
Proceedings of the International Conference on Applied Science and Health No. 3 (2018)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Common Indonesians often use alternative therapy for Diabetes Mellitus (DM) due to its high prevalence. Tree marigold (Tithonia difersivolia (Hemsl.) A. Gray) leaves show its positive effect in decreasing blood glucose level. Thus, it is important to analyze the negative effect of consuming the proposing alternative including its toxicity level to vital organs. This study aims to observe the blood glucose level and liver histopathology in streptozotocin-induced diabetic male rats treated by infusion and ethanol extract of the tree marigold leaves. Methods: Pretest and posttest with control group design experiment was conducted to analyze effect on blood glucose level and posttest only with control group design was for determining liver histopathology. Twenty-four male white rats (Wistar strain) were randomly divided into 4 groups: normal control group (KN), positive control group (K+) treated with 0.45mg/kg of glibenclamid, treatment group 1 (P1) treated by 100 mg/kg of tree marigold leaves ethanol extract, and treatment group 2 (P2) treated by 1373 mg/kg of tree marigold leaves infusion. The rats at the groups of K+, P1, and P2 were induced by streptozotocin to reach hyperglycemic state. Comparison of blood glucose level effect between infusion and ethanol extract was analyzed by One-Way ANOVA continued by post-hoc test, while comparison of liver histopathology effect was determined by Kruskal-Wallis continued by Mann-Whitney test. Results: Significant differences of blood glucose level decrease were found in all groups. Group P2 significantly decreased blood glucose level more than P1 (p=0.000) as much as 124.97 mg/dl and 101.46 mg/dl respectively. Significant differences of liver histopathology were obtained between KN vs K(+), KN vs P2, K(+) vs P2, and P1 vs P2. Group P2 showed more severe of liver histopathology changes than P1 (p=0.025). Conclusion: Tree marigold leaves extract is more effective in lowering blood glucose level and has less toxic effect to liver histopathology than its infusion. 
EFFECT OF PHYSICAL ACTIVITY AND FAST FOOD CONSUMPTION FREQUENCY ON OVERWEIGHT LEVEL AMONG HIGH SCHOOL ADOLESCENT Lita Harlianti; Witri Pratiwi; Donny Nauphar; Eni Suhaeni
Proceedings of the International Conference on Applied Science and Health No. 3 (2018)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Obesity was believed to be one of the risk factors of degenerative diseases, such as diabetes mellitus, hypertension, and coronary heart disease. Obesity occurred gradually that started with overweight. Risk factors for overweight are lack of physical activity, and consumption of high caloric fatty food which may cause accumulation of fats inside the body. This research aims to examine the effect of physical activity and fast food consumption frequency on overweight level among high school adolescent in Cirebon. Methods: It was a cross-sectional study conducted among 262 high school students aged 15-17 years using stratified random sampling. Results: The result based on Spearman correlation test showed negative correlation between physical activity and overweight level with p= 0,001 (p< 0,05) and correlation coefficient of -0,627. There was positive correlation between consumption fast food frequency with overweight with p=0,037 (p=0,05) and correlation coefficient of 0,423. Conclusions: Physical activity and fast food consumption frequency have effects on overweight level in high school adolescent in Cirebon. 
CALCULATING COLONY OF Candida spp. AMONG CHILDREN WITH INTELLECTUAL DISABILITY IN CIREBON Dhini Oktaviani; Amanah Amanah; Eni Suhaeni; Risnandya Primanagara
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Children and adults with Intellectual Disability and Autism Spectrum Disorders (ASD) have decreased intestinal permeability and enzyme activity, causing gastrointestinal (GI) problems that are more frequent and more severe than in children from the general population. The immune system which is part of the intestinal barrier in children with Intellectual Disability (ID) experiences various disorders and becomes more susceptible to fungal invasion, such as Candida spp. This present study aims to calculate the colony of Candida spp. in the digestive tract of Children with Intellectual Disability (ID) in the city of Cirebon.Method: This is an observational study with cross-sectional design using purposive sampling. The study was conducted in November 2018-February 2019. The sample (ID) group consists of 31 individuals from Special Needs School and 60 individuals from National elementary school (non-ID) in the city of Cirebon. The inclusion criteria were children ages 6-12 years and the exclusion criteria were children under antifungal treatment and children under cytotoxic or immunosuppressive drugs.  The fecal samples from the ID group and non-ID were examined at the Laboratory of the Faculty of Medicine, Universitas Swadaya Gunung Jati Cirebon, Indonesia using Digital Colony counter. Collected data were stored and analyzed using computer aided Statistical program. Mann-Whitney analysis of variance techniques were used to test the hypotheses. Results: The results showed that there are significant differences in the colonies of Candida spp. between the ID group and non-ID group. Median colony of Candida spp. in the ID group is 39.00 with mean colony of 126.90 while the median colony of non-ID group is 3.00 with mean colony of 40.93 (p<0.001).Conclusion: There are significant differences between the Candida spp. colonies in the ID group with the non-ID.
CALCULATING COLONY OF Candida spp. AMONG CHILDREN WITH INTELLECTUAL DISABILITY IN CIREBON Dhini Oktaviani; Amanah Amanah; Eni Suhaeni; Risnandya Primanagara
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Children and adults with Intellectual Disability and Autism Spectrum Disorders (ASD) have decreased intestinal permeability and enzyme activity, causing gastrointestinal (GI) problems that are more frequent and more severe than in children from the general population. The immune system which is part of the intestinal barrier in children with Intellectual Disability (ID) experiences various disorders and becomes more susceptible to fungal invasion, such as Candida spp. This present studyaims to calculate the colony of Candida spp. in the digestive tract of Children with Intellectual Disability (ID) in the city of Cirebon. Method: This is an observational study with cross-sectional design using purposive sampling. The study was conducted in November 2018-February 2019. The sample (ID) group consists of 31 individuals from Special Needs School and 60 individuals from National elementary school (non-ID) in the city of Cirebon. The inclusion criteria were children ages 6-12 years and the exclusion criteria were children under antifungal treatment and children under cytotoxic or immunosuppressive drugs. The fecalsamples from the ID group and non-ID were examined at the Laboratory of the Faculty of Medicine, Universitas Swadaya Gunung Jati Cirebon, Indonesia using Digital Colony counter. Collected data were stored and analyzed using computer aided Statistical program. Mann-Whitney analysis of variance techniques were used to test the hypotheses. Results: The results showed that there are significant differences in the colonies of Candida spp. between the ID group and non-ID group. Median colony of Candida spp. in the ID group is 39.00 with mean colony of 126.90 while the median colony of non-ID group is 3.00 with mean colony of 40.93(p<0.001). Conclusion: There are significant differences between the Candida spp. colonies in the ID group with the non-ID. Keywords: Intellectual Disability (ID), Candida spp., Gastrointestinal
EFFECT OF TREE MARIGOLD (Tithonia diversifolia (HEMSL.) A. GRAY) LEAVES INFUSION AND ETHANOL EXTRACT ON BLOOD GLUCOSE LEVEL AND LIVER HISTOPATHOLOGY OF STREPTOZOTOCIN-INDUCED MALE WISTAR RATS Rachma Ayu Maulidiany; Eni Suhaeni; Catur Setiya Sulistiyana; Ariestya Indah Permata Sari
Proceedings of the International Conference on Applied Science and Health No. 3 (2018)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Common Indonesians often use alternative therapy for Diabetes Mellitus (DM) due to its high prevalence. Tree marigold (Tithonia difersivolia (Hemsl.) A. Gray) leaves show its positive effect in decreasing blood glucose level. Thus, it is important to analyze the negative effect of consuming the proposing alternative including its toxicity level to vital organs. This study aims to observe the blood glucose level and liver histopathology in streptozotocin-induced diabetic male rats treated by infusion and ethanol extract of the tree marigold leaves. Methods: Pretest and posttest with control group design experiment was conducted to analyze effect on blood glucose level and posttest only with control group design was for determining liver histopathology. Twenty-four male white rats (Wistar strain) were randomly divided into 4 groups: normal control group (KN), positive control group (K+) treated with 0.45mg/kg of glibenclamid,treatment group 1 (P1) treated by 100 mg/kg of tree marigold leaves ethanol extract, and treatment group 2 (P2) treated by 1373 mg/kg of tree marigold leaves infusion. The rats at the groups of K+, P1, and P2 were induced by streptozotocin to reach hyperglycemic state. Comparison of blood glucose level effect between infusion and ethanol extract was analyzed by One-Way ANOVA continued by post-hoc test, while comparison of liver histopathology effect was determined by Kruskal-Wallis continued byMann-Whitney test. Results: Significant differences of blood glucose level decrease were found in all groups. Group P2 significantly decreased blood glucose level more than P1 (p=0.000) as much as 124.97 mg/dl and 101.46 mg/dl respectively. Significant differences of liver histopathology were obtained between KN vs K(+), KN vs P2, K(+) vs P2, and P1 vs P2. Group P2 showed more severe of liver histopathology changes than P1 (p=0.025). Conclusion: Tree marigold leaves extract is more effective in lowering blood glucose level and has less toxic effect to liver histopathology than its infusion.
EFFECT OF PHYSICAL ACTIVITY AND FAST FOOD CONSUMPTION FREQUENCY ON OVERWEIGHT LEVEL AMONG HIGH SCHOOL ADOLESCENT Lita Harlianti; Witri Pratiwi; Donny Nauphar; Eni Suhaeni
Proceedings of the International Conference on Applied Science and Health No. 3 (2018)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Obesity was believed to be one of the risk factors of degenerative diseases, such as diabetes mellitus, hypertension, and coronary heart disease. Obesity occurred gradually that started with overweight. Risk factors for overweight are lack of physical activity, and consumption of high caloric fatty food which may cause accumulation of fats inside the body. This research aims to examine the effect of physical activity and fast food consumption frequency on overweight level among high school adolescent in Cirebon. Methods: It was a cross-sectional study conducted among 262 high school students aged 15-17 years using stratified random sampling. Results: The result based on Spearman correlation test showed negative correlation between physical activity and overweight level with p= 0,001 (p< 0,05) and correlation coefficient of -0,627. There was positive correlation between consumption fast food frequency with overweight with p=0,037 (p=0,05) and correlation coefficient of 0,423. Conclusions: Physical activity and fast food consumption frequency have effects on overweight level in high school adolescent in Cirebon.