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All Journal Journal of Oceanography BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Edukasi dan Penelitian Informatika (JEPIN) Padjadjaran Journal of Dentistry Jurnal Meteorologi Klimatologi dan Geofisika JURNAL PENDIDIKAN TAMBUSAI Unes Law Review YUME : Journal of Management Aptisi Transactions on Technopreneurship (ATT) TIN: TERAPAN INFORMATIKA NUSANTARA Jurnal Penelitian Pendidikan, Psikologi Dan Kesehatan (J-P3K) Journal of Economics and Business UBS Jurnal Disrupsi Bisnis Jurnal Pengabdian Kepada Masyarakat Jurnal Ekonomi Trisakti Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi E-Jurnal Akuntansi TSM Jurnal Ilmiah MEA (Manajemen, Ekonomi, dan Akuntansi) Jurnal Komunikasi, Sains dan Teknologi (JKST) Hexatech: Jurnal Ilmiah Teknik Jurnal Indonesia Sosial Teknologi The International Journal of Medical Science and Health Research The Indonesian Journal of General Medicine Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)
Stephanie Stephanie
Universitas Tanjungpura
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Veterinary Other

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Introduction of the Serine Green Fluorescent Protein (sGFP) Gene into Pyricularia grisea Race dc4 Isolated from Digitaria ciliaris using Agrobacterium tumefaciens-mediated Genetic Transformation Stephanie, Stephanie; Widyastuti, Utut; Wiyono, Suryo
BIOTROPIA Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.1.329

Abstract

Blast disease (caused by Pyricularia grisea) has long been known as a serious problem for upland rice, and recently, it has also begun infecting lowland rice. However, the mechanism enabling this range expansion is still unknown. One possible method to study this shift is by inserting a marker gene into P. grisea so that its spread can be monitored. The sGFP (Synthetic Green Fluorescent Protein) gene has been widely used as a reporter to track gene expression and cellular processes in fungi. In this study, the sGFP gene was integrated into the genome of P. grisea isolate DC4 from Digitaria ciliaris. The sGFP plasmid was first introduced into Agrobacterium tumefaciens using the triparental mating method (TPM). Genetic transformation was then carried out by co-cultivating spores of P. grisea DC4 with A. tumefaciens strain LBA4404 harboring the pCAMBIA-sGFP construct. Transformants were selected on medium containing 300 µg/mL hygromycin. Successful integration of the sGFP gene into the fungal genome was confirmed using PCR with sGFP-specific primers, an sGFP–Nos terminator primer pair, and a β-tubulin primer pair as an internal control. Fluorescent microscopy using blue light excitation confirmed sGFP expression in the P. grisea DC4 transformant.
A Comprehensive Bibliometric Study of Digital Leadership Influence on Technopreneurial Success Gunawan, Ahmad; Hasyim, Wachid; Putih, Muhadar; Wirjawan, Tri Wahyu; Gopar, Ida Abdul; Stephanie, Stephanie
Aptisi Transactions On Technopreneurship (ATT) Vol 7 No 2 (2025): July
Publisher : Pandawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34306/att.v7i2.498

Abstract

This study investigates the role of digital leadership in fostering technopreneurship in Indonesia, a rapidly evolving sector shaped by technological advancements and digital transformation. As technological innovation and data-driven decision-making are central to the success of technopreneurs, understanding the role of leadership in this context is critical for sustainable growth. The primary aim of this research is to explore how digital leadership can enhance technopreneurship in Indonesia by promoting collaboration, innovation, and operational efficiency. The method a literature review and bibliometric analysis are employed to identify key trends and research patterns in digital leadership and its impact on the technopreneurship ecosystem. By analyzing data from various sources, the study provides an in-depth examination of the current state of the field. This research contributes a unique perspective by combining bibliometric analysis with traditional literature review, providing a comprehensive, data-driven approach to understanding how digital leadership drives technological innovation. It also emphasizes the challenges technopreneurs face, such as the digital skills gap and regulatory barriers, areas often overlooked in previous studies. While previous research has explored the general impact of digital leadership on business transformation, this study fills the gap by specifically focusing on Indonesia technopreneurship ecosystem and its unique challenges. The findings confirm that digital leadership plays a pivotal role in overcoming obstacles like unequal access to technology and driving innovation in technopreneurship. The study recommends strategies such as enhancing digital skills, investing in infrastructure, and fostering more flexible regulations to support the growth of technopreneurship in Indonesia.
TPACK Based Differentiated Problem Posing to Enhance Entrepreneurial Digital Problem Solving Derlina, Derlina; Zuhra, Siti; Mihardi, Satria; Lubis, Rajo Hasim; Purba, John Tampil; Stephanie, Stephanie
Aptisi Transactions On Technopreneurship (ATT) Vol 7 No 3 (2025): November
Publisher : Pandawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34306/att.v7i3.609

Abstract

This study aims to determine the effect of TPACK-based differentiated learning on high school students' problem-solving abilities and motivation. The study, conducted at Private Senior High School PAB 8 Saentis Sei Tuan, used a quasi-experiment method with two classes: the experimental class, which used the differentiated problem solving model based on TPACK, and the control class, which used the conventional school learning model without differentiation. The results of this study indicate that the average post-test score for student problem-solving abilities in the experimental class was higher than in the control class. Statistical testing using two-way ANOVA showed that the problem-solving skills of students taught with the TPACK-based differentiated learning model demonstrated a significant influence between the learning model and motivation on students' problem-solving skills, as well as an interaction between the TPACK based problem posing learning model and motivation in terms of problem-solving skills. These findings have practical implications for educators, providing evidence that the TPACK-based differentiated problem-posing learning model is more effective in improving students' problem solving abilities than the conventional learning model, with learning motivation also playing an essential role in enhancing student problem-solving skills.
Co-Authors Ade Maulana Alifia, Marini Arafah, Nadiya Nur Astuti, Dessy Ayu Aulia Oktaviani Ayu Trisna Hayati, Ayu Trisna Barus, Okky Putra Cantika, Chindy Caroline, Gabriella Dinna Cuandra, Fendy Davidsen Cuaca Delicia, Freja Derlina . Emi Sukarti, Emi Endang Sukartini, Endang Febriyani, Yundha Geovedi, Ferdinand Gunawan, Ahmad Hasana, Elinda Nurul Hendra Hendra Henry Kurnia Setiawan Herry Sujaini I Putu Gede Dharma Eka, I Putu Gede Dharma Eka Ida Abdul Gopar Jacelyin, Jacelyin Jayadi, Devina Jimawan, Owen Nixon Jovin Kendrico Kahar, Neysa Marcella Kamilatunnaimah, Mutiara Khaw, Brian Kurnia, Angelica Nathania Livera, Silva Luki, Marko Martin, Kevin Muhammad Rizky Pribadi Namira Ufrida Rahmi Natalia Wijaya, Natalia Wijaya Nazmi, Hendra Owen Felix Pramita, Nina Prapanca, Cherin Purba, John Tampil Putih, Muhadar Putri, Herviana Haruko Tadia Rajo Hasim Lubis Rianda Elvinawanty Riza Rahardiawan Rosita Darianty Satria Mihardi Shevia, Shevia Siagian, Mauli Sihombing, Marya A.Y. Sri Tiatri Stefanny, Stefanny Sugeng Widada Surbakti, Anton Diary Steward Suryo Wiyono Syamantha, Arbana Tan, Cristina Tanujaya, Priscillia Paramitha Tunaimah, Mutiara Kamila UTUT WIDYASTUTI Vanesia Roselin Vinanda, Vinanda Virgiansyah, Muhammad Rifqi Wachid Hasyim, Wachid Werner R Murhadi Winar Joko Alexander Wirjawan, Tri Wahyu Zai, Immanuel Ziven Louis Zubaidi, Umar Issa Zuhra, Siti