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HISTOLOGI HATI MENCIT (Mus musculus L.) YANG DIBERI EKSTRAK DAUN LAMTORO (Leucaena leucocephala) I Wayan Andi Yoga Kurniawan; Ngurah Intan Wiratmini; Ni Wayan Sudatri
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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Leucaena (Leucaena leucocephala) has been used as traditional medicine, such asdrugs to decrease the level of blood glucose, so it can be used as drug to indicatediabetes. The aim of this research is to study the histopathological change of mice’s livergiven leucaena leaf extract. This research used 24 male mice that were divided into 4 groupsby completely randomized design. Group P0 (control) were given 0,9 % NaCl, and groupsP1, P2, and P3 were given 0,5 g / kg bw, 1 g / kg bw, and 1,5 g / kg bw leucaena’s leafextract by oral administration respectively. The treatment were given daily for 30 days. All ofthe mice were necropsied at day 31, and the liver were taken to examine theirhistopathological change. Histopathological change examination were based on the presenthydropic degeneration, fatty degeneration, and apoptosis surround central vein. Result ofstatistically analised by ANOVA and Kruskal-Wallis method indicated no significancedifference among the control and treatments. There was no significance toxicity effect ofleucaena leaf extract of all treatment dosage on mice liver.Keywords: Leucaena leucocephala, liver histology, Mus musculus L.

PENGARUH PEMBERIAN EKSTRAK TAUGE DITAMBAH MADU TERHADAP KUALITAS SPERMATOZOA MENCIT JANTAN (Mus musculus L.) I Wayan Wena Diartha; Ni Wayan Sudatri; Iriani Setyawati
SIMBIOSIS Vol 4, No 1 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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Bean sprouts, vegetables that are often consumed, contain vitamin E, C, riboflavin, niacin, folate, and some minerals suchas selenium, manganese, zinc and iron. Honey contains monosaccharide i.e. fructose, sucrose, glucose, and maltose. Thisstudy aimed to investigate the effect of bean sprout extract added with honey to the spermatozoa quality of male mice and themost concentration that affected. This research used a completely randomized design (CRD). Twenty male mice divided intofour groups: control (aquadest), P1 (25 mg/g BW bean sprout extract + 0.07 ml honey), P2 (50 mg/g BW bean sprout extract+ 0.07 ml honey) and P3 (75 mg/g BW bean sprout extract + 0.07 ml honey). The extracts were given 0.3 ml/mice/day for 30days. The variables that are observed were spermatozoa motility, morphology, viability and also amount of spermatozoa. Datawere analyzed with one way ANOVA and followed by Duncan Multiple Range Test (DMRT). The results showed that beansprout extracts added with honey gave positive effects by increasing type A motility of spermatozoa but decreased type Dmotility. The treatments also increased the amount of spermatozoa significantly. However, 75 mg/g BW bean sprout extract +0.07 ml honey did not affect spermatozoa morphology and viability of male mice (Mus musculus L.). The optimal dose thataffected sperm quality in this research was 50 mg/g BW bean sprout extract + 0.07 ml honey.Keywords : honey, Mus musculus L, bean sprout extracts, the quality of spermatozoa

AKTIVITAS ANTIDIABETES EKSTRAK ETANOL DAUN BELIMBING BESI (Averhhoa carrambola) PADA MENCIT (Mus musculus) NI PUTU SRI PUSPITA WIDI YANTHI; Ni Putu Adriani Astiti; Ni Wayan Sudatri
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Senyawa antioksidan, flavonoid, dan saponin banyak terkandung dalam daun belimbing besi (Averrhoa carambola) sehingga tujuan dari penelitian ini untuk melihat pengaruh antidiabetes ekstrak daun belimbing besi pada mencit. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 6 perlakuan, yaitu P1 (aquadest tanpa aloksan) sebagai kontrol negatif, P2 (aquadest dengan aloksan) sebagai control positif, P3 metformin 500 mg/kgBB), P4 (aloksan + ekstrak daun belimbing besi 100 mg/kgBB) , P5 (aloksan + ekstrak daun belimbing besi 150 mg/kgBB), P6 (aloksan + ekstrak daun belimbing besi 200 mg/kgBB). Sebelumnya hewan coba dijadikan diabetes dengan menginduksi aloksan sebanyak 120 mg/kgBB secara intramuscular pada otot paha. Pembuatan ekstrak daun belimbing besi menggunakan metode maserasi. Setelah hewan diabetes, diberikan perlakuan ekstrak etanol daun belimbing besi sesuai rancangan percobaan, dimulai hari ke-7 sampai hari ke-21. Kadar glukosa darah diukur dengan glucometer setiap minggu. Hasil penelitian menunjukkan nilai penurunan kadar glukosa darah terbaik terjadi pada minggu pertama dan minggu kedua. Pada minggu pertama, perlakuan metformin mampu menurunkan glukosa darah mencit sebanyak 88.4%, sedangkan perlakuan ekstrak etanol daun belimbing besi dosis 200 mg sebanyak 60%. Pada minggu kedua, 65.8% untuk metformin, dan 63% untuk dosis ekstrak daun belimbing 200 mg/kg BB. Ekstrak etanol daun belimbing besi berpotensi sebagai obat antidiabetes. Kata Kunci: mencit, diabetes mellitus, glukosa darah, daun belimbing besi.

SPECIES OF CAPUNG (ORDER ODONATA) IN RICE PLANTS IN SEVERAL SAWAH SEKITAR DENPASAR, BALI Ni Made Suartini; Ni Wayan Sudatri
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Dragonfly is an insect which belongs to the Odonata order and consists of the suborder Anisoptera (dragonflies) and the suborder Zygoptera (damselflies). Its habitat is very wide including in the rice field. Dragonflies and damselflies are predatory insects, both in the form of nymphs and adults, so their role is very important in maintaining the balance of the ecosystem. The research was conducted to finding out the dragonfly species found in rice plantations in rice fields around the Denpasar area. Sampling was done by catching using insect nets on rice plantations, in the morning from 08.00 to 11.00 WITA, and in the afternoon from 15.00 to 17.00 WITA. Dragonfly samples obtained were then preserved to be identified by observing the morphological characters based on Hanum et al. (2013), and Sigit et al. (2013). Dragonfly species found in rice plantations areas in several rice fields around Denpasar are as many as 8 species which are included in 2 suborder, namely suborder Anisoptera (5 species) and suborder Zygoptera (3 species), Species of suborder Anisoptera (Potamarcha congener) only found in rice plantations in East Denpasar. Keywords: insects, dragonfly habitat, identification, morphological characters

Pemberian Ekstrak Etanol Pelepah Batang Pisang Ketip Dapat Meringankan Polycystic Ovary Syndrome pada Tikus Putih Model Kadek Mardika; Ni Wayan Sudatri; Sang Ketut Sudirga
Jurnal Veteriner Vol 23 No 2 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Polycystic ovary syndrome is a condition that affects the fertility of female reproductive organs. ketip banana pseudostem extract contains flavonoids, saponins, alkaloids, and vitamin C. It is known that ketip banana pseudostem extract can trigger cell proliferation and contain phytoestrogen compound. The purpose of this study was to determine the ability of ketip banana pseudostem extract on length of estrus cycle, histology structure and wight of reproductive organ, and estrogen hormone levels in white rats of PCOS model. White rats female Wistar strain aged 54 days used a total of 25 animals in this study. The treatment was given for 21 days by 1 ml/tail/day with the gavage method. Vaginal smear was began on 5th day of treatment every 12 hours/day for three estrus cycles (15 days). At the end of the study (21th day), the rats were dissected to take their ovaries and blood. The ovaries were weighed and then histological preparation with paraffin method and hematoxylin-Eosin staining. The parameters observed were length of estrus cycle, weight of ovary, number of ovary follicles, as well as estrogen hormone levels. The result of this study showed that ketip banana pseudostem extract can extend proestrus phases and estrus phases, increase estrogen hormone levels, increase corpus luteum number as significantly (p<0.05) on 150 mg/kgBW dosage.

Daya proteksi minyak atsiri bunga kenanga (Cananga odorata ) dalam sediaan lotion antinyamuk terhadap Aedes aegypti Putu Ayudina Asti Puspita; Ni Luh Arpiwi; Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 26 No 2 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i02.p12

Berbagai alternatif telah dilakukan untuk menghambat populasi nyamuk Aedes aegypti penyebab penyakit demam berdarah, diantaranya penggunaan lotion antinyamuk yang mengandung DEET. Untuk mengurangi resiko dan dampak negatif dari lotion antinyamuk yang mengandung DEET, maka salah satu upaya dilakukan pemanfaatan minyak atsiri kenanga (Cananga odorata) sebagai repellent atau antinyamuk alami. Penelitian ini bertujuan untuk mengetahui rendemen minyak atsiri bunga kenanga, untuk menganalisis konsentrasi minyak atsiri pada sediaan lotion yang memberikan daya proteksi tertinggi terhadap gigitan nyamuk Ae. aegypti, menganalisis tingkat kesukaan probandus terhadap formulasi lotion, dan untuk mengetahui ada tidaknya sensitivitas setelah memakai lotion yang mengandung minyak atsiri bunga kenanga. Minyak atsiri kenanga pada penelitian ini diperoleh dengan cara destilasi uap yang selanjutnya digunakan sebagai agen antinyamuk dalam sediaan lotion. Rata-rata rendemen minyak atsiri bunga kenanga dengan tiga kali ulangan diikuti dengan standar deviasi (SD) adalah 0,63% ± 1,38 b/b. Daya proteksi lotion antinyamuk tertinggi adalah lotion F3 dengan konsentrasi minyak atsiri 5%. Lotion F3 pada jam pertama memberikan daya proteksi sebesar 96,3%, jam kedua 92,3%, jam ketiga 87% dan menurun sampai jam keenam menjadi 75%. Formulasi yang paling disukai oleh probandus adalah lotion dengan kandungan minyak atsiri sebanyak 5% b/b. Setelah pemakaian lotion antinyamuk tidak ada kesan lengket dikulit dan tidak adanya gejala sensitivitas.

MORPHOMETRY OF EAGLE SPECIES (FAMILY ACCIPITRIDAE) AT BALI BIRD PARK Iriani Setyawati; L.P. Eswaryanti Kusuma Yuni; Retno Kawuri; Ni Made Suartini; Ni Wayan Sudatri; I Gede Agus Pradana Putra
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

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This study measured the morphometry of raptor species (Family Accipitridae) in Bali Bird Park (BBP) namely Nisaetus cirrhatus (Changeable Hawk Eagle), Elanus caeruleus (Black Winged Kite), Spilornis cheela (Crested Serpent Eagle) and Haliaeetus leucogaster (White Bellied Sea Eagle). Measurements were made with meter tape and caliper on birds that were in their resting period. Raptor at BBP generally rest for 6 months until experience molting before being retrained for 4-5 months, then the birds will be ready to perform bird show for the next 9-12 months. Our measurements obtained the largest bird was Haliaeetus leucogaster with a total body length of 75 cm, wings length 83.5-85 cm, tail length 26 cm, head size (8 cm length and 7 cm width), upper beak (8 cm length and 2.5 cm thick) and lower beak (3 cm length and 1 cm thick), lower limb length (femur 17 cm, tibia 9 cm, metatarsus 11 cm, middle toe 6.5 cm and grip 9 cm). The smallest bird was Elanus caeruleus with a total body length of 33 cm, wing length 39-45 cm, tail length 19 cm, head size (8 cm length, 5.5 cm width), upper beak (2.5 cm length and 0.5 cm thick) and lower beak (1 cm length and 0.3 cm thick), lower limb length (femur 8 cm, tibia 4 cm, metatarsus 5 cm, middle toe 3 cm and grip 5 cm) The morphometry of the species Nisaetus cirrhatus and Spilornis cheela were between the two other bird species.

MORPHOMETRY OF EAGLE SPECIES (FAMILY ACCIPITRIDAE) AT BALI BIRD PARK Iriani Setyawati; L.P. Eswaryanti Kusuma Yuni; Retno Kawuri; Ni Made Suartini; Ni Wayan Sudatri; I Gede Agus Pradana Putra
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v3i3.5733

This study measured the morphometry of raptor species (Family Accipitridae) in Bali Bird Park (BBP) namely Nisaetus cirrhatus (Changeable Hawk Eagle), Elanus caeruleus (Black Winged Kite), Spilornis cheela (Crested Serpent Eagle) and Haliaeetus leucogaster (White Bellied Sea Eagle). Measurements were made with meter tape and caliper on birds that were in their resting period. Raptor at BBP generally rest for 6 months until experience molting before being retrained for 4-5 months, then the birds will be ready to perform bird show for the next 9-12 months. Our measurements obtained the largest bird was Haliaeetus leucogaster with a total body length of 75 cm, wings length 83.5-85 cm, tail length 26 cm, head size (8 cm length and 7 cm width), upper beak (8 cm length and 2.5 cm thick) and lower beak (3 cm length and 1 cm thick), lower limb length (femur 17 cm, tibia 9 cm, metatarsus 11 cm, middle toe 6.5 cm and grip 9 cm). The smallest bird was Elanus caeruleus with a total body length of 33 cm, wing length 39-45 cm, tail length 19 cm, head size (8 cm length, 5.5 cm width), upper beak (2.5 cm length and 0.5 cm thick) and lower beak (1 cm length and 0.3 cm thick), lower limb length (femur 8 cm, tibia 4 cm, metatarsus 5 cm, middle toe 3 cm and grip 5 cm) The morphometry of the species Nisaetus cirrhatus and Spilornis cheela were between the two other bird species.

Kandungan vitamin C dan antioksidan lulur bunga telang (Clitoria ternatea L.) Yusni Khusnita Pangesti; Sang Ketut Sudirga; Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 27 No 2 (2023): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2023.v27.i02.p10

Kesehatan kulit merupakan salah satu hal yang berhubungan dengan kecantikan yang dapat dirawat dengan menggunakan kosmetik lulur. Lulur dapat dibuat dengan memanfaatkan bahan alami, salah satunya yang memiliki antioksidan almi seperti vitamin C. Tanaman yang berpotensi sebagai bahan lulur adalah kembang telang (Clitoria ternatea L.) karena memiliki kadar vitamin C dan nilai IC50 yang cukup tinggi. Tujuan penelitian ini adalah untuk mengetahui kandungan vitamin C dan antioksidan pada serbuk bunga telang dan sediaan lulur bunga telang, serta mengetahui persepsi responden terhadap lulur bunga telang. Rancangan penelitian yang digunakan untuk uji vitamin C dan antioksidan lulur adalah Rancangan Acak Lengkap (RAL) dengan 6 variasi lulur serta 3 pengulangan. Dilakukan juga uji organoleptik, dan uji hedonik. Uji vitamin C dilakukan dengan metode Titrasi dan uji antioksidan dengan menggunakan metode DPPH. Hasil penelitian dianalisis secara statistika menggunakan uji ANOVA dan apabila terdapat perbedaan nyata maka dilanjutkan dengan menggunakan Uji Duncan. Hasil analisis ditampilkan dalam bentuk tabel. Kandungan vitamin C pada serbuk bunga telang sebesar 1,41 mg/100g dan IC50 sebesar 118,25 ppm dengan kategori sedang. Kandungan vitamin C lulur bunga telang selaras dengan nilai aktivitas antioksidannya dengan hasil tertinggi terdapat pada lulur dengan kombinasi serbuk beras 75% dan serbuk bunga telang 25%, sedangkan terendah pada lulur serbuk beras 95% dan serbuk bunga telang 5%. Lulur yang paling disukai adalah lulur dengan kombinasi serbuk beras 75% dan serbuk bunga telang 25%.

Angka Lempeng Total (ALT), Coliform, Escherichia coli dan Staphylococcus aureus pada urutan babi di wilayah Kecamatan Banjarangkan, Bali Komang Nikastri Tussning Dewi; Ida Bagus Gede Darmayasa; Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 28 No 1 (2024): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2024.v28.i01.p12

Urutan atau sosis bali merupakan produk pangan tradisional yang banyak diminati dan dijual di provinsi Bali. Pengolahan, penanganan dan penyimpanan yang kurang tepat akan mengakibatkan kontaminasi mikroba penyebab keracunan makanan diantaranya Coliform, Escherichia coli, dan Staphylococcus aureus. Tujuan penelitian ini untuk mengetahui total cemaran bakteri Angka Lempeng Total (ALT), Coliform, Escherichia coli, dan Staphylococcus aureus pada urutan babi yang dijual di Kecamatan Banjarangkan, Bali. Pengambilan sampel diperoleh dari pencatatan pedagang urutan babi di Kecamatan Banjarangkan menggunakan metode purposive sampling. Parameter yang diuji adalah ALT dan Staphylococcus aureus dengan metode sebar (pour plate), Coliform dengan metode Most Probable Number (MPN) dan Escherichia coli dengan metode gores (streak plate). Kandungan Angka Lempeng Total (ALT) dan Staphylococcus aureus berfluktuasi antara 102 – 105 CFU/g dalam rentang 3 minggu pengujian, 7 dari 15 sampel positif tercemar Coliform, 15 sampel tercemar Escherichia coli dibawah < 3 × 102 MPN/100g. Sebanyak 12 sampel dari 15 sampel (80%) tidak layak dikonsumsi untuk uji ALT, 8 dari 15 sampel (53,3%) layak konsumsi untuk uji Coliform, 12 sampel dari 15 sampel (80%) layak konsumsi untuk uji S. aureus, serta seluruh sampel layak konsumsi untuk uji E. coli sesuai standar baku mutu yang ditetapkan oleh BPOM No. 13 Tahun 2019 dan SNI 7388:2009.

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