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All Journal International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari Ecotrophic, Journal of Environmental Science SIMBIOSIS Jurnal Riau Biologia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Riset Biologi dan Aplikasinya Indonesian Biodiversity Journal Indonesian Biodiversity Journal
Ni Wayan Sudatri
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

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HISTOLOGICAL OF MICE MAMMAL GLANDS THAT SUPPLEMENTATION WITH INFUSE WATER OF MANIHOT ESCULENTA LEAVES Warmadewi, I Gusti Ayu Intan; Rai Suarni, Ni Made; Sudatri, Ni Wayan
SIMBIOSIS Vol 11 No 2 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i02.p07

Abstract

Fitoestrogen merupakan senyawa dari tumbuhan yang memiliki kemiripan struktur dan memiliki efek seperti estrogen. Flavonoid merupakan salah satu jenis senyawa fitoestrogen. Daun ubi kayu diketahui mengandung senyawa flavonoid. Penelitian dilakukan untuk mengetahui pengaruh seduhan daun ubi kayu (Manihot esculenta Crantz.) terhadap gambaran histologi kelenjar mammae mencit (Mus musculus) betina. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan dan 6 kali ulangan. Perlakuan terdiri dari K0 sebagai kontrol diberi akuades 0,5 mL/bb/hr, P1 yang diberi seduhan daun ubi kayu 0,5 mL dengan konsentrasi 7%, P2 yang diberi seduhan daun ubi kayu 0,5 mL dengan konsentrasi 9%, P3 yang diberi seduhan daun ubi kayu 0,5 mL dengan konsentrasi 11% selama 21 hari. Pada hari ke-22 dilakukan pembedahan, kemudian kelenjar mammae mencit (M. musculus) betina diambil untuk dibuat sayatan histologi. Sayatan histologi kelenjar mammae mencit dibuat dengan metode parafin dan pewarnaan hematoxylyn-eosin serta diamati dibawah mikroskop dengan perbesaran 100x dengan bantuan optilab dan program image raster. Parameter yang diamati meliputi rata-rata jumlah alveoli kelenjar mammae, jumlah duktus kelenjar mammae, serta diameter duktus kelenjar mammae mencit (M. musculus) betina. Data yang didapat dianalisis secara statistik dengan program SPSS seri 23 for Windows. Apabila dari hasil analisis berbeda nyata maka dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa seduhan daun ubi kayu secara nyata meningkatkan rata-rata jumlah alveoli, jumlah duktus dan diameter duktus kelenjar mammae mencit (Mus musculus) betina. Dosis seduhan daun ubi kayu (M. esculenta Crantz.) yang paling berpengaruh adalah dosis 9 g/Kg BB mencit (M. musculus) betina.
Chronic Toxicity Effect of Mimosa pudica Leaf Extract Towards Histology Profile of Stomach and Duodenum in Mice Andayani, Ni Kadek Prederika Sari; Setyawati, Iriani; Sudatri, Ni Wayan
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 2 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n2.p52-59

Abstract

In Indonesia, Mimosa pudica Linn. (M. pudica L.) is a wild plant (weed) from the Mimosaceace family. This plant is widely used as a traditional medicine for various types of diseases such as insomnia, acute eye inflammation, urolithiasis (urinary stones), fever, and bronchitis. This study aimed to find out the effect of M. pudica leaf extract on the histological damage of the stomach and duodenum of mice. This study used a Completely Randomized Design with 24 male mice, which were divided into control placebo (P0) given CMC-Na 0.5%; and M. pudica leaf extract doses of 200 mg/kg body weight (P1); 400 mg/kg body weight (P2); and 600 mg/kg body weight (P3). The extract was administered orally for 28 days. On day 29, the animal was dissected to collect its digestive organs. The histological preparation of the stomach and duodenum of mice was performed by using the paraffin method and Hematoxylin and Eosin staining to observe the histological damage, namely congestion, hemorrhage, epithelial cells desquamation, inflammatory cell infiltration, edema, and hyperplasia. Quantitative data were analyzed with a one-way ANOVA test and Duncan's post hoc test with the SPSS version 22. The results showed that the M. pudica leaf extract increased hemorrhage and hyperplasia damage at doses of 400 and 600 mg/kg body weight in mice’s duodenum.
In vitro antifungal activity of trembesi leaf extract [Samanea saman (Jacq.) Merr.] against Colletotrichum magnum Rossman & Allen, the causal agent of papaya anthracnose Ratnasari, Athalia; Darmadi, Anak Agung Ketut; Inabuy, Fainmarinat selviani; Sudatri, Ni Wayan
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225317-327

Abstract

Papaya fruit production in Bali has decreased over the last three years (2020-2022). One of the contributing factors is infection by Colletotrichum spp., which causes anthracnose. Anthracnose is an airborne disease that infects various plant commodities including papaya. The use of chemical fungicides to control anthracnose can have negative impacts if used long-term. Trembesi [Samanea saman (Jacq.) Merr.] extract has previously been reported to possess antifungal, antiseptic, antibacterial, and antidiabetic properties. This study aimed to determine the effectiveness and category of inhibitory activity, Minimum Inhibitory Concentration (MIC), Lethal Concentration 50% (LC??), and the phytochemical content of trembesi leaves. Methanol extract of trembesi leaves effectively inhibited the growth of Colletotrichum magnum at a concentration of 5%. The experiment used 11 treatments with 4 replications, including control (0% v/v) and extract concentrations of 1% to 10% (v/v), along with a positive control. The corresponding inhibition zone diameters were: 0.00 mm, 12.00 mm, 11.87 mm, 13.62 mm, 16.00 mm, 19.62 mm, 15.75 mm, 16.87 mm, 17.87 mm, 17.87 mm, 18.25 mm, and 20.87 mm, respectively. The minimum concentration of extract showing inhibitory activity (MIC) was 0.3%, while the LC?? value was 0.32%. Phytochemical screening revealed the presence of phenolics, tannins, alkaloids, steroids, terpenoids, flavonoids, and saponins in the trembesi leaf extract.
Co-Authors A.A.S. A. Sukmaningsih Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Andayani, Ni Kadek Prederika Sari Dwi Ariani Yulihastuti G.A.A Rina Febrina I G.A.P. Adnyana I G.S. Astawa I Gede Agus Pradana Putra I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Ketut Ginantra I KETUT MUKSIN I M. Widiartha I Putu Ari Wijana Dipa I Wayan Andi Yoga Kurniawan I Wayan Wena Diartha I. Harini I.G.A.S. Wahyuni Ida Bagus Gede Darmayasa Ida Bagus Gede Darmayasa Inabuy, Fainmarinat selviani IRIANI SETYAWATI Kadek Indah Kartika Sari Kadek Mardika Komang Nikastri Tussning Dewi LUH Puru ESWARYANTI KUSUMA YUNI MADE PHARMAWATI Ngurah Intan Wiratmini Ni G. A. Manik Ermayanti Ni LUH ARPIWI Ni Luh Watiniasih Ni Made Suartin Ni Made Suartini Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Sinta Puspa Dewi NI PUTU SRI PUSPITA WIDI YANTHI Putu Ayudina Asti Puspita Rai Suarni, Ni Made Ratnasari, Athalia Retno Kawuri Salsabillah Rahma SANG KETUT SUDIRGA Warmadewi, I Gusti Ayu Intan Yusni Khusnita Pangesti