Articles
<![CDATA[Biodegradasi Alkil Benzena Sulfonat oleh Psedomonas cepacia ]]>
<![CDATA[Sudiana, I Made]]>
<![CDATA[ JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA ]]>
Publisher : <![CDATA[Perhimpunan Biologi Indonesia ]]>
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DOI: 10.14203/jbi.v3i3.3469
<![CDATA[ABSTRACTAlkyl Benzene Sulfonate Biodegradation of Psedomonas cepacia. Alkyl Benzene Sulfonate (ABS), naturally slow biodegradable substances, and toxic to human, animal and microorganisms, is a focus of environmental studies. Microorganisms appeared to play important role on biodegradation of that substances in nature, and wastewater treatment procesess. S2 isolated from detergent contaminated soil was able to grow in media with ABS as the sole carbon source. ABS degradation took place under aerobic condition, at pH 4, temperature 30ºC with ?max of 0.0591-h, Ks = 3.25 mg/L, Vmax = 0.16 mg/L.hours-1, and Km = 14.52 mg/L. Analyses of 16s rDNA revealed that S2 is belonging to Pseudomonas cepacia.Key words: Alkyl Benzene Sulfononate (ABS), Pseudomonas cepacia, detergent]]>
<![CDATA[Production of Acid Phosphatase in Bacillus sp. Isolated from Forest Soil of Gunung Salak National Park ]]>
<![CDATA[Rahmansyah, Maman]]>;
<![CDATA[Sudiana, I Made]]>
<![CDATA[ JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA ]]>
Publisher : <![CDATA[Perhimpunan Biologi Indonesia ]]>
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DOI: 10.14203/jbi.v6i3.3140
<![CDATA[ABSTRAKProduktivitas Fosfatase Asam pada Bacillus sp. yang Diisolasi dari Tanah Hutan TamanNasional Gunung Salak. Pada pengamatan ini dilakukan karakterisrik bakteri pelarut fosfatyang diisolasi dari tanah hutan Taman Nasional Gunung Salak. Sebanyak 21 koloni hasil isolasidiuji terhadap produktivitas enzim fosfatase berdasar pelarutan media mengandung fosfat.Isolat yang terkuat melarutkan fosfat diidentifikasi sebagai Bacillus sp. Pada pengamatanlanjutan terhadap strain teruji dilakukan penumbuhan pada media cair selama 90 jam inkubasi,dan hasilnya ternyata mampu melarutkan fosfat inorganik (Pi) dari sumber trikalsium fosfat(Ca-Pi) dan alumunium fosfat (Al-Pi) masing-masing pada kisaran 1,2 sampai 152 dan 0.8 sampai25 mg.L-1; dan menunjukkan aktifitas enzim fosfomonoesterase antara 0.2 sampai 1.01 unitpada media yang mengandung larutan para-nitrophenylphosphate sebagai media fosfatorganik (Po) artifisial. Konsumsi glukosa pada media yang diukur selama pertumbuhan sejalanpula dengan produk ortofosfat sebagai akibat adanya aktifitas enzim fosfatase. Peningkatanfosfatase juga sejalan dengan bertambahnya biomassa sel bakteri dan penambahan produkasam glukonat. Penurunan pH dari 7 menjadi 5 diakibatkan peningkatan produk asam glukonatdi dalam media tumbuh. Bakteri pelarut fosfat yang berasal dari tanah hutan Taman NasionalGunung Salak dapat memproduksi fosfatase asam untuk memineralisasi sumber-sumber fosfatmenjadi sumber nutrisi yang siap digunakan oleh akar tumbuhan, dan itu merupakan prediksikuat untuk menjadikan isolat bakteri pelarut fosfat sebagai sumber bahan pupuk hayati.Kata kunci: Bacillus sp., tanah hutan, fosfatase asam, Ca-Pi, Al-Pi.]]>
<![CDATA[Ragam Aktivitas Urease dan Fosfomonoesterase serta Perannya dalam Ketersediaan Nutrisi N dan P pada Tanah Kebun Biologi Wamena ]]>
<![CDATA[Rahmansyah, Maman]]>;
<![CDATA[Latupapua, H.J.D.]]>;
<![CDATA[Sudiana, I Made]]>
<![CDATA[ JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA ]]>
Publisher : <![CDATA[Perhimpunan Biologi Indonesia ]]>
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DOI: 10.14203/jbi.v3i4.3325
<![CDATA[ABSTRACTDiscrepancy of urease and phosphomonoesterase activities and its role in establishing Nand P nutrition in soil collected from Wamena Biological Research Station. Microbialactivities in soil lead to know for establishing soil nutrient status. Accordingly, soil collectedfrom Biological Research Station in Wamena then sent to the laboratory and determined on itsenzymatic activities and the physicochemical, as well. In this work, the enzymatic activities ofurease and phosphomonoesterase were examined in relation with soil microbial respiration, inorder to understand the mineralization of nitrogenous and phosphorus compound in soil. Soilrespiration rate (2.43-3.21 mg C02 g-dm12hour) designated variation in each sample, as wellas urease (8.6-23.5 unit/g soil) and phosphomonoesterase (5.5-7.9 unit/g soil) activities.Phosphomonoesterase activity showed strong correlation with respiration rate within soil; andreveal to the configuration of the bioactivities and physicochemical soil figures concluded thatthe B sample has the poor fertility. The phenomenon of data fulfill that bioactivities hadcorrelation with the physicochemical compound in the soil.Keywords: respiration, urease, phosphomonoesterase, Wamena Biological Research Station.]]>
<![CDATA[Increase of Citric Acid Production by Aspergillus niger InaCC F539 in Sorghum’s Juice Medium Amended with Methanol ]]>
<![CDATA[Kanti, Atit]]>;
<![CDATA[Ilyas, Muhammad]]>;
<![CDATA[Sudiana, I Made]]>
<![CDATA[ JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA ]]>
Publisher : <![CDATA[Perhimpunan Biologi Indonesia ]]>
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DOI: 10.14203/jbi.v14i2.3733
<![CDATA[ABSTRACTCitric acid demand increases steadily, and there is a need to increase productivity through selection of suitable carbon sources, and addition of substances that increase citric acids production rate. Methanol has been suggested to increase citric acid fermentation on high carbohydrate containing substances. The objective of the study was to evaluate the suitability of sweet sorghum juice for citric acids production and to verify the effect of methanol on citric acids production using Aspergillus niger InaCC F539 as inoculant. Sweet sorghum juice with the total initial reducing sugar of 11.5 % (w/v) was used as the sole carbon sources. To study the effect of total initial reducing sugar on citric acid production the initial reducing sugar was adjusted to the concentration of 30 to 75 g/L. Preliminary experiment was conducted to get the optimum methanol concentration that stimulate citric acid production. The optimum methanol concentration that stimulate citric acid production was 4% (v/v). Submerged fermentation was conducted as shake culture (125 rpm at 28 °C). Citric acids production was affected by total initial reducing sugar. Higher total initial reducing sugar produced higher citric acids. Maximum citric acid production was 18.96g/L on sweet sorghum juice with 75 g/L total initial reducing sugar. Methanol 4 % (v/v) increase citric acid production by 41.35 to 65.89 %. Juice of sweet sorghum was a good medium for citric acids production, and methanol stimulate and increase citric acid production. It is a good basis for exploring efficient and cost effective industrial scale citric acid production. Keywords: Citric acid, Methanol, Sweet sorghum, Aspergillus niger ]]>
<![CDATA[Identification of Ectomycorrhiza-Associated Fungi and Their Ability in Phosphate Solubilization ]]>
<![CDATA[Mujahidah, Shofia]]>;
<![CDATA[Sukarno, Nampiah]]>;
<![CDATA[Kanti, Atit]]>;
<![CDATA[Sudiana, I Made]]>
<![CDATA[ JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA ]]>
Publisher : <![CDATA[Perhimpunan Biologi Indonesia ]]>
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DOI: 10.14203/jbi.v14i2.3741
<![CDATA[ABSTRACTThe existence of Pinus sp. is very dependent on ectomycorrhizal (ECM) fungi. ECM fungi affect the growth of their hosts especially by increasing mineral availability and water intake. However ECM fungi is not the only one that plays a role in the growth of their host. There are many ECM-associated fungi which also have many important roles in the growth of the host. Helotiales which were isolated from the ECM of Pinus merkusii are known as the most member of root associated fungi. Three isolated Helotiales identified as Scytalidium sp., Helotiales sp., and Glutinomyces sp. by morphological and molecular identification based on ITS1, 5.8S rRNA, ITS2 DNAr region. All three isolates have the ability to solubilize phosphate. Compared with C. geophilum which already known as P solubilizing fungi, Glutinomyces solubilized 16.6 ppm P which is higher than C. geophilum which solubilized as much as 13.68 ppm in Pikosvkaya medium with glucose as carbon source and rock phosphate as phosphate source. Then followed by Scytalidum sp. and lastly Helotiales sp. Rock phosphate tend to harder to solubilize because its complex chemical form with other minerals. Keyword: ECM-associated fungi, Helotiales, phosphate solubilizing ability, Pinus merkusii ]]>
<![CDATA[RAGAM AKTIVITAS UREASE DAN FOSFOMONOESTERASE SERTA PERANNYA DALAM KETERSEDIAAN NUTRISI N DAN P PADA TANAH KEBUN BIOLOGI WAMENA ]]>
<![CDATA[Rahmansyah, Maman]]>;
<![CDATA[Latupapua, H.J.D.]]>;
<![CDATA[Sudiana, I Made]]>
<![CDATA[ JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA ]]>
Publisher : <![CDATA[Perhimpunan Biologi Indonesia ]]>
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DOI: 10.14203/jbi.v3i4.3325
<![CDATA[ABSTRACTDiscrepancy of urease and phosphomonoesterase activities and its role in establishing Nand P nutrition in soil collected from Wamena Biological Research Station. Microbialactivities in soil lead to know for establishing soil nutrient status. Accordingly, soil collectedfrom Biological Research Station in Wamena then sent to the laboratory and determined on itsenzymatic activities and the physicochemical, as well. In this work, the enzymatic activities ofurease and phosphomonoesterase were examined in relation with soil microbial respiration, inorder to understand the mineralization of nitrogenous and phosphorus compound in soil. Soilrespiration rate (2.43-3.21 mg C02 g-'dm12hour) designated variation in each sample, as wellas urease (8.6-23.5 unit/g soil) and phosphomonoesterase (5.5-7.9 unit/g soil) activities.Phosphomonoesterase activity showed strong correlation with respiration rate within soil; andreveal to the configuration of the bioactivities and physicochemical soil figures concluded thatthe B sample has the poor fertility. The phenomenon of data fulfill that bioactivities hadcorrelation with the physicochemical compound in the soil.Keywords: respiration, urease, phosphomonoesterase, Wamena Biological Research Station.]]>
<![CDATA[IDENTIFICATION OF ECTOMYCORRHIZA-ASSOCIATED FUNGI AND THEIR ABILITY IN PHOSPHATE SOLUBILIZATION ]]>
<![CDATA[Mujahidah, Shofia]]>;
<![CDATA[Sukarno, Nampiah]]>;
<![CDATA[Kanti, Atit]]>;
<![CDATA[Sudiana, I Made]]>
<![CDATA[ JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA ]]>
Publisher : <![CDATA[Perhimpunan Biologi Indonesia ]]>
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DOI: 10.14203/jbi.v14i2.3741
<![CDATA[ABSTRACTThe existence of Pinus sp. is very dependent on ectomycorrhizal (ECM) fungi. ECM fungi affect the growth of their hosts especially by increasing mineral availability and water intake. However ECM fungi is not the only one that plays a role in the growth of their host. There are many ECM-associated fungi which also have many important roles in the growth of the host. Helotiales which were isolated from the ECM of Pinus merkusii are known as the most member of root associated fungi. Three isolated Helotiales identified as Scytalidium sp., Helotiales sp., and Glutinomyces sp. by morphological and molecular identification based on ITS1, 5.8S rRNA, ITS2 DNAr region. All three isolates have the ability to solubilize phosphate. Compared with C. geophilum which already known as P solubilizing fungi, Glutinomyces solubilized 16.6 ppm P which is higher than C. geophilum which solubilized as much as 13.68 ppm in Pikosvkaya medium with glucose as carbon source and rock phosphate as phosphate source. Then followed by Scytalidum sp. and lastly Helotiales sp. Rock phosphate tend to harder to solubilize because its complex chemical form with other minerals. Keyword: ECM-associated fungi, Helotiales, phosphate solubilizing ability, Pinus merkusii ]]>
<![CDATA[PRODUCTION OF ACID PHOSPHATASE IN BACILLUS SP. ISOLATED FROM FOREST SOIL OF GUNUNG SALAK NATIONAL PARK ]]>
<![CDATA[Rahmansyah, Maman]]>;
<![CDATA[Sudiana, I Made]]>
<![CDATA[ JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA ]]>
Publisher : <![CDATA[Perhimpunan Biologi Indonesia ]]>
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DOI: 10.14203/jbi.v6i3.3140
<![CDATA[ABSTRAKProduktivitas Fosfatase Asam pada Bacillus sp. yang Diisolasi dari Tanah Hutan TamanNasional Gunung Salak. Pada pengamatan ini dilakukan karakterisrik bakteri pelarut fosfatyang diisolasi dari tanah hutan Taman Nasional Gunung Salak. Sebanyak 21 koloni hasil isolasidiuji terhadap produktivitas enzim fosfatase berdasar pelarutan media mengandung fosfat.Isolat yang terkuat melarutkan fosfat diidentifikasi sebagai Bacillus sp. Pada pengamatanlanjutan terhadap strain teruji dilakukan penumbuhan pada media cair selama 90 jam inkubasi,dan hasilnya ternyata mampu melarutkan fosfat inorganik (Pi) dari sumber trikalsium fosfat(Ca-Pi) dan alumunium fosfat (Al-Pi) masing-masing pada kisaran 1,2 sampai 152 dan 0.8 sampai25 mg.L-1; dan menunjukkan aktifitas enzim fosfomonoesterase antara 0.2 sampai 1.01 unitpada media yang mengandung larutan para-nitrophenylphosphate sebagai media fosfatorganik (Po) artifisial. Konsumsi glukosa pada media yang diukur selama pertumbuhan sejalanpula dengan produk ortofosfat sebagai akibat adanya aktifitas enzim fosfatase. Peningkatanfosfatase juga sejalan dengan bertambahnya biomassa sel bakteri dan penambahan produkasam glukonat. Penurunan pH dari 7 menjadi 5 diakibatkan peningkatan produk asam glukonatdi dalam media tumbuh. Bakteri pelarut fosfat yang berasal dari tanah hutan Taman NasionalGunung Salak dapat memproduksi fosfatase asam untuk memineralisasi sumber-sumber fosfatmenjadi sumber nutrisi yang siap digunakan oleh akar tumbuhan, dan itu merupakan prediksikuat untuk menjadikan isolat bakteri pelarut fosfat sebagai sumber bahan pupuk hayati.Kata kunci: Bacillus sp., tanah hutan, fosfatase asam, Ca-Pi, Al-Pi.]]>
<![CDATA[INCREASE OF CITRIC ACID PRODUCTION BY ASPERGILLUS NIGER INACC F539 IN SORGHUM’S JUICE MEDIUM AMENDED WITH METHANOL ]]>
<![CDATA[Kanti, Atit]]>;
<![CDATA[Ilyas, Muhammad]]>;
<![CDATA[Sudiana, I Made]]>
<![CDATA[ JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA ]]>
Publisher : <![CDATA[Perhimpunan Biologi Indonesia ]]>
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DOI: 10.14203/jbi.v14i2.3733
<![CDATA[ABSTRACTCitric acid demand increases steadily, and there is a need to increase productivity through selection of suitable carbon sources, and addition of substances that increase citric acids production rate. Methanol has been suggested to increase citric acid fermentation on high carbohydrate containing substances. The objective of the study was to evaluate the suitability of sweet sorghum juice for citric acids production and to verify the effect of methanol on citric acids production using Aspergillus niger InaCC F539 as inoculant. Sweet sorghum juice with the total initial reducing sugar of 11.5 % (w/v) was used as the sole carbon sources. To study the effect of total initial reducing sugar on citric acid production the initial reducing sugar was adjusted to the concentration of 30 to 75 g/L. Preliminary experiment was conducted to get the optimum methanol concentration that stimulate citric acid production. The optimum methanol concentration that stimulate citric acid production was 4% (v/v). Submerged fermentation was conducted as shake culture (125 rpm at 28 °C). Citric acids production was affected by total initial reducing sugar. Higher total initial reducing sugar produced higher citric acids. Maximum citric acid production was 18.96g/L on sweet sorghum juice with 75 g/L total initial reducing sugar. Methanol 4 % (v/v) increase citric acid production by 41.35 to 65.89 %. Juice of sweet sorghum was a good medium for citric acids production, and methanol stimulate and increase citric acid production. It is a good basis for exploring efficient and cost effective industrial scale citric acid production. Keywords: Citric acid, Methanol, Sweet sorghum, Aspergillus niger ]]>
<![CDATA[BIODEGRADASI ALKIL BENZENA SULFONAT OLEH PSEDOMONAS CEPACIA ]]>
<![CDATA[Sudiana, I Made]]>
<![CDATA[ JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA ]]>
Publisher : <![CDATA[Perhimpunan Biologi Indonesia ]]>
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DOI: 10.14203/jbi.v3i3.3469
<![CDATA[ABSTRACTAlkyl Benzene Sulfonate Biodegradation of Psedomonas cepacia. Alkyl Benzene Sulfonate (ABS), naturally slow biodegradable substances, and toxic to human, animal and microorganisms, is a focus of environmental studies. Microorganisms appeared to play important role on biodegradation of that substances in nature, and wastewater treatment procesess. S2 isolated from detergent contaminated soil was able to grow in media with ABS as the sole carbon source. ABS degradation took place under aerobic condition, at pH 4, temperature 30ºC with ?max of 0.0591-h, Ks = 3.25 mg/L, Vmax = 0.16 mg/L.hours-1, and Km = 14.52 mg/L. Analyses of 16s rDNA revealed that S2 is belonging to Pseudomonas cepacia.Key words: Alkyl Benzene Sulfononate (ABS), Pseudomonas cepacia, detergent]]>
