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Identification of Saponins from the Active Fraction Sea Cucumbers (Stichopus vastus sluiter) Bintang, M. Irwan Sapta Purna; Sukmiwati, Mery; Karnila, Rahman
Jurnal Perikanan dan Kelautan Vol. 28 No. 1 (2023): February
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This study aims to determine the secondary metabolic content of saponin contained in the active fraction of sea cucumber (Stichopus vastus) by using metabolic test reagents and thin-layer chromatography (TLC) analysis. The active fraction was given the reagent in the form of HCl 2N and strong acid then continued with analysis to determine the Rf value and lieberman-burchad reaction. The results of the metabolic reaction test showed that the active fraction contained steroidal saponin compounds characterized by the presence of a stable foam and the formation of a greenish-green color. TLC analysis produced a single stain with an Rf value of 0.9 and a bluish color reaction after being given Liberman-burchad which confirmed that the saponins contained in the active fraction of sea cucumbers were steroidal saponin.
Functional Groups of Scale Collagen from Farmed Snakehead Fish (Channa striata) and Wild Sources Agustina, Ayu; Hasan, Bustari; Sukmiwati, Mery
Jurnal Perikanan dan Kelautan Vol. 28 No. 2 (2023): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The study aimed to analyze the functional groups of scale collagen from farmed snakehead fish and wild sources. Scale collagen from farmed snakehead fish was higher than the wild sources (18.82 > 14.27%) with the protein content of both successively (60.24 > 59.78%). The functional groups in scale collagen from farmed snakehead fish were Amide A (3668,77 cm-1), Amide B (2920,35 cm-1) Amide I (2302,14 cm-1), Amide II (1446,67 cm-1), and Amide III (1245,10 cm-1). Meanwhile, scale collagen from wild sources snakehead fish has an Amide A group (3853.94 cm-1), Amide B (2905.89 cm-1), and Amide I (1869.10 cm-1) Amide II (1457.28 cm-1), Amide III (1243.18 cm-1). Scale Collagen from farmed snakehead fish and wild sources is classified as type 1 collagen
Identification of Saponins from the Active Fraction Sea Cucumbers (Stichopus vastus sluiter) Bintang, M. Irwan Sapta Purna; Sukmiwati, Mery; Karnila, Rahman
Jurnal Perikanan dan Kelautan Vol. 28 No. 1 (2023): February
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This study aims to determine the secondary metabolic content of saponin contained in the active fraction of sea cucumber (Stichopus vastus) by using metabolic test reagents and thin-layer chromatography (TLC) analysis. The active fraction was given the reagent in the form of HCl 2N and strong acid then continued with analysis to determine the Rf value and lieberman-burchad reaction. The results of the metabolic reaction test showed that the active fraction contained steroidal saponin compounds characterized by the presence of a stable foam and the formation of a greenish-green color. TLC analysis produced a single stain with an Rf value of 0.9 and a bluish color reaction after being given Liberman-burchad which confirmed that the saponins contained in the active fraction of sea cucumbers were steroidal saponin.
Functional Groups of Scale Collagen from Farmed Snakehead Fish (Channa striata) and Wild Sources Agustina, Ayu; Hasan, Bustari; Sukmiwati, Mery
Jurnal Perikanan dan Kelautan Vol. 28 No. 2 (2023): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The study aimed to analyze the functional groups of scale collagen from farmed snakehead fish and wild sources. Scale collagen from farmed snakehead fish was higher than the wild sources (18.82 > 14.27%) with the protein content of both successively (60.24 > 59.78%). The functional groups in scale collagen from farmed snakehead fish were Amide A (3668,77 cm-1), Amide B (2920,35 cm-1) Amide I (2302,14 cm-1), Amide II (1446,67 cm-1), and Amide III (1245,10 cm-1). Meanwhile, scale collagen from wild sources snakehead fish has an Amide A group (3853.94 cm-1), Amide B (2905.89 cm-1), and Amide I (1869.10 cm-1) Amide II (1457.28 cm-1), Amide III (1243.18 cm-1). Scale Collagen from farmed snakehead fish and wild sources is classified as type 1 collagen
Aktivitas antikanker senyawa bioaktif dari Paracaudina australis: Studi in silico yang menargetkan AKT1 dan NOS2 Sukmiwati, Mery; Susilawati, Susilawati; Islami, Deri
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 28 No. 8 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(8)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/m8s22h81

Abstract

Sea cucumber isolates (Paracaudina australis) have shown potential as agents with various bioactivities. Bioactivity refers to the ability of a substance to interact with biological tissues and elicit positive physiological responses. This study aimed to analyze the interaction between the target proteins RAC-alpha serine/threonine-protein kinase (AKT1) and Nitric Oxide Synthase 2 (NOS2) with compounds identified in P. australis. The methods employed included ligand preparation using the Open Babel 2.3.1 plug-in, bioactivity prediction via the PASS Online web server, ADMET analysis using Canonical SMILES, pathway prediction using the DAVID (Database for Annotation, Visualization, and Integrated Discovery) web server, prediction of active or functional sites in proteins using BIOVIA Discovery Studio software, molecular docking using AutoDock Vina integrated in PyRx 0.8, visualization with BIOVIA Discovery Studio 2019, and molecular dynamics simulation using the WebGro web server. The results revealed that the P. australis sea cucumber isolate contained 38 compounds predicted to exhibit bioactivities related to the target proteins, including anticancer, anti-proliferative, pro-apoptotic, anti-inflammatory, anti-type 2 diabetes, and anti-obesity effects. Based on five ADMET parameters, all compounds satisfied several pharmacokinetic and toxicity criteria. Protein interaction analysis indicated that AKT1 and NOS2 interacted with other proteins, such as MTCP1, TCL1A, and NOX1. Molecular docking results showed that the compounds Ergosta-7,22-dien-3-ol, (3β,5α,22E); Ergostan-3-ol; Ergost-7-en-3-ol, (3β,5α); and Stigmasta-7,16-dien-3-ol, (3β,5α) exhibited the most stable binding to AKT1. Additionally, the compounds Ergosta-7,22-dien-3-ol, (3β,5α,22E) and Ergosta-7,22-dien-3-ol, (3β,22E) demonstrated lower binding affinity values than the control NOS2 inhibitor, indicating strong potential as NOS2 inhibitors.
OPTIMALISASI USAHA BLADO SNACK MELALUI AHLI TEKNOLOGI DIVERSIFIKASI DAN FORTIFIKASI IKAN PATIN DI BANGKINANG Sukmiwati, Mery; Leksono, Tjipto; Sari, N Ira
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol. 29 No. 4 (2023): OKTOBER-DESEMBER
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkm.v29i4.48580

Abstract

Hasil dari pelatihan dan penyuluhan pada masyarakat mitra UKM Blado Snack ditemukan beberapa permasalahan yang selanjutnya dicarikan solusinya. Diantaranya, kurang memadainya alat yang digunakan dalam pengolahan, sehingga mengakibatkan terbatasnya jumlah produksi dan sekaligus mengakibatkan terbatasnya jangkauan pemasaran produk Blado Snack. Selain itu, kurangnya pengetahuan dan pemahaman tentang Cara Pengolahan Pangan yang Baik (CPPB-IRT) serta manajemen wirausaha mikro yang terintegrasi. Metode yang dilakukan dalam pengabdian ini adalah kualitatif dengan mengumpulkan data melalui, wawancara, observasi, penyuluhan, pelatihan, dan pendampingan terhadap mitra UKM. Kesimpulannya peserta pelatihan mendapatkan pengetahuan dan pemahaman tentang CPPB-IRT dan manajemen wirausaha mikro yang terintegrasi. Dan juga mitra mendapatkan peralatan pengolahan yang mendukung usaha. Dari hasil kegiatan pengabdian mitra sudah dapat memperluas pemasaran dengan mengikuti stand di beberapa pusat perbelanjaan di Pekanbaru.
Pengaruh Suhu Pemanasan terhadap Karakteristik Garam Fungsional dari Rumput Laut Cokelat (Sargassum aquifolium) Silaban, Deni Saputra; Sukmiwati, Mery; Diharmi, Andarini
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 1 (2024): Juni 2024
Publisher : Politeknik - Ahli Usaha Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v19i1.966

Abstract

Sargassum aquifolium adalah rumput laut cokelat berpotensi sebagai bahan baku garam fungsional. Penelitian ini bertujuan untuk menentukan pengaruh suhu pemanasan berbeda terhadap karakteristik garam fungsional S. aquifolium. Metode yang digunakan adalah eksperimen menggunakan Rancangan Acak Lengkap (RAL) non faktorial. Perlakuan terdiri atas suhu pemanasan berbeda 25°C, 30°C, dan 35°C dengan 3 kali pengulangan. Parameter analisis terdiri atas rendemen, kadar natrium, kalium, NaCl, komponen bioaktif, dan aktivitas antioksidan. Rendemen garam S. aquifolium yang dihasilkan dengan proses diektraksi pada suhu 25°C, 30°C, dan 35°C berturut-turut sebesar 3,73%; 5,93%; dan 8,01%. Kadar natrium dan kalium garam S. aquifolium pada pemanasan dengan suhu 25°C, 30°C dan 35°C berturut-turut adalah; 1,92 mg/g; 2,29 mg/g dan 2,55 mg/g (Na), dan 39,18 mg/g; 42,64 mg/g dan 46,00 mg/g (K). Kadar NaCl yang dihasilkan dengan suhu pemanasan (25, 30, dan 25°C) sebesar 13,43%; 10,37% dan 7,95%. Senyawa bioaktif garam S. aquifolium terdiri atas fenolik, flavonoid, steroid dan saponin. Aktivitas antioksidan pada garam S. aquifolium dengan pemanasan suhu 25°C, 30°C dan 35°C diperoleh nilai IC50 berturut-turut 139,10 ppm, 147,34 ppm, dan 194.40 ppm. Perlakuan suhu pemanasan terbaik pada proses ektraksi S. aquifolium adalah 35°C, garam yang dihasilkan memiliki karakteristik rendemen 8,01%, kadar natrium 2,55 mg/g, kalium 46,00 mg/g, NaCl 7,95%. Senyawa bioaktif yang diperoleh terdiri dari fenolik, flavonoid, steroid, dan saponin. Aktivitas antioksidan garam S. aquifolium dengan nilai IC50 sebesar 194,40 ppm.AbstractSargassum aquifolium is a brown seaweed that has potential as a raw material for functional salt. This research aimed to determine the effect of different heating temperatures on the functional salt characteristics of S. aquifolium. The method used was an experiment using a non-factorial Completely Randomized Design (CRD). The treatment consisted of different heating temperatures of 25°C, 30°C, and 35°C with 3 repetitions. The analysis parameters consist of yield, content of sodium, potassium, NaCl, bioactive compound, and antioxidant activity. The yield of S. aquifolium salt produced by the extraction process at temperatures of 25°C, 30°C, and 35°C was 3.73%; 5.93%; and 8.01%. The sodium and potassium content of S. aquifolium salt when heated to a temperature of 25°C, 30°C, and 35°C were; 1.92 mg/g; 2.29 mg/g, and 2.55 mg/g (Na) and 39.18 mg/g; 42.64 mg/g and 46.00 mg/g (K). The NaCl content produced by heating temperatures (25, 30, and 25C) was 13.43%; 10.37%, and 7.95%. The bioactive compounds of S. aquifolium salt consist of phenolics, flavonoids, steroids, and saponins. The antioxidant activity of S. aquifolium salt by heating at 25°C, 30°C, and 35°C obtained IC50 values of 139.10 ppm, 147.34 ppm, and 194.40 ppm respectively. The best heating temperature treatment in the S. aquifolium extraction process was 35°C. The resulting salt has a yield characteristic of 8.01%, sodium content 2.55 mg/g, potassium 46.00 mg/g, and NaCl 7.95%. The bioactive compounds are phenolics, flavonoids, steroids, and saponins. The antioxidant activity of S. aquifolium salt has an IC50 value of 194.40 ppm.Keywords: antioxidant, functional salt, sodium, NaCl, potasium, Sargassum aquifolium
Potensi antioksidan dari teripang berunok (Paracaudina australis): Antioxidant potency of transparent sea cucumber (Paracaudina australis) Sukmiwati, Mery; Karnila, Rahman; Putri, Dea Arsifah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 27 No. 2 (2024): Jurnal Pengolahan Hasil Perikanan Indonesia 27(2)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v27i2.46969

Abstract

Teripang kaya akan senyawa aktif yang berpotensi sebagai sumber antioksidan. Salah satu jenis teripang yang ditemukan di Indonesia adalah berunok (Paracaudina australis). Faktor yang memengaruhi hasil ekstraksi di antaranya waktu ekstraksi, jenis pelarut, dan jumlah sampel. Tujuan dari penelitian ini yaitu menentukan waktu ekstraksi terbaik pada berunok melalui parameter rendemen dan aktivitas penghambatan antioksidan. Ekstraksi berunok dilakukan pada waktu yang berbeda, yaitu 48, 72, dan 96 jam dengan pelarut metanol. Parameter yang dianalisis meliputi identifikasi proporsi berunok, rendemen, uji fitokimia, dan aktivitas antioksidan melalui metode Ferric Reducing Antioxidant Power (FRAP). Analisis data yang digunakan yaitu rancangan acak lengkap (RAL) dengan perlakuan waktu ekstraksi berbeda. Hasil penelitian menunjukkan proporsi bagian tubuh berunok yaitu isi perut 88%, bagian daging dan kulit 12%. Berunok terdeteksi memiliki senyawa aktif flavonoid, terpenoid, steroid, saponin, alkaloid, dan fenolik. Waktu ekstraksi selama 96 jam merupakan perlakuan terbaik dengan nilai rendemen tertinggi sebesar 7,90% dan aktivitas antioksidan dengan nilai IC50 648,24 ppm. Aktivitas antioksidan yang terdapat pada berunok dengan perlakuan waktu ekstraksi berbeda tergolong sangat lemah.
Pelatihan Pengembangan Program Kewirausahaan Berbasis Teknologi Diversifikasi Ikan Patin Sebagai Produk Hilirisasi Menuju Komersialisasi Pada Kelompok Kewirausahan Fisheries Product Club Suparmi Suparmi; Sumarto Sumarto; Windarti Windarti; Mery Sukmiwati; Lapeti Sari; Rizky Febriansyah Siregar; Nofri Sandria; Ranika Paramita
CANANG: Jurnal Pengabdian Masyarakat Vol 5, No 2 (2025)
Publisher : PELANTAR PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/canang.v5i2.69

Abstract

The Science and Technology-Based Entrepreneurship Development Program (PPKBI) plays a crucial role as a form of downstream research translation towards commercialization. This community engagement initiative is expected to enhance collaboration with partners, in this case, a student entrepreneurship group known as the Fisheries Product Club (FPC). The downstream products have already been granted simple patents, namely Ubikan Fish Nugget (Patent No. IDS000004954) and Anekarasa Catfish Macaroni (Patent No. IDS000005784). The objective of this program is to provide opportunities to accelerate the development of a culture of utilizing university research outputs for the benefit of the community, as well as to foster cooperation with partners, including industry and marketing sectors. The implementation method employed the Participatory Action Learning System (PALS), which is fundamentally based on engaging students in an active and naturally participatory learning process within the entrepreneurship program through training. This approach forms an interactive, participatory learning system, both at the individual and group levels. A total of 20 participants, all members of the FPC, took part in the program. The training yielded positive responses and motivated participants to embark on entrepreneurial activities. The products, Anekarasa catfish macaroni and Ubikan fish nuggets, hold strong potential to be developed as viable business opportunities for emerging entrepreneurs
Co-Authors ', Dahlia ', Desmelati ', Febriandi ', Megawati Ade Olga Dinayah June Adlita Eska Putri Afra Azizah Afriadi Sinaga Alik, Agustinus Tato’ Andarini Diharmi Andi Almer Farrand Noor Angki Angelo Delevio Anita Roza Antonius Zulqaiman Anugrah, Rio Persal Arlika Pebri, Arlika Ayu Agustina Azizah, Afra Bintang, M. Irwan Sapta Purna Bunga M.W Bunga M.W Bustari Hasan Catur Wirawan Chairul Chairul Coki Randi Natama, Coki Randi Dahlia ' Dahlia Dahlia Depri Gusriadi Desi Heltina Desmelati ' Dewita Buchari dhumoli_tampubolon dhumoli_tampubolon Dian Handayani Dimas Prasetia Drastinawati Drastinawati Edison Edison Edison Edison Emma Susanti Enda Mora Faira Yovanie Farkhan, Mochammad Farrand Noor, Andi Almer Fazira, Meisya Amanda Febi Nabila Febriani Melisa Sinurat Febrigusni, Audia Fionasari, Dwi Fryathama, Ilham Ganda Aliem Pakarsya Lubis Henni Syawal Herdini , Herdini Heriansyah, Ivan Hotmeriah Padang Ika Darmila Siregar Imam Gusdika Rahman Imam Kristof Octaviaman. H Ira N Sari Ira Sari Irvan Ibrahim Islami, Deri Jan Stepfely Simanjuntak Karnila, Rahman Karnila Kembaren, Bella Billiant J Ananta Lapeti Sari Lily Afriyanti Liza Mulya Sari M. Alhadid M. Alhadid Mardianto Hidayat Martahan Gabema S Meisya Amanda Fazira Mhd. Ridho Putra Mhd. Taufik Harahap Mirna Ilza Mochammad Farkhan Mohamad Irvan Ibrahim Mohd. Ramadhani MST Siagian, Roy Muhammad Raihan Muhammad Zakiyul Fikri Munisatul Haq N Ira Sari N. Ira Sari N. Ira Sari N. Ira Sari, N. Ira Sari N. Nurjanah Nabellia Nabellia Nabellia, Nabellia Nabila, Febi Nadia Adilla Nefri Nefri Nita Grecia Damanik Nofri Sandria Nofri Sandria NURHAYATI ' Paradise, Nadia Pendi Parsiholan Gultom Pradina Purwati Putri Noviayu Salsabila Hendri Putri, Dea Arsifah Putri, Indah Prasetya PUTRIANA SARI SIRAIT Rafika Monaliza Ranika Paramita Reinhard Nuary M. Ambarita Rio Persal Anugrah Rizkha Yana Pratiwi, Rizkha Yana Roy Susanto Salsabila Hendri, Putri Noviayu Salsabila, Unik Hanifah Santhy Wisuda Sidauruk Santika, Jesi Sanusi Ibrahim Sari, Noor Ira Sayyidina Ahmad Zhariif Selpiana Sihombing Septin Alvin Mendrofa Shafa Tasya Rinanda Sherrin Sherrin Silaban, Deni Saputra Simanjuntak, Jan Stepfely Siregar, Riri Andriani Siregar, Rizky Febriansyah Siti Kholijah Siti Nurjanah Siti Salmah Suhardiyanto Suhardiyanto Sumarto Sumarto ' Sumarto Sumarto Sumarto Sumarto Sumarto, Sumarto Suparmi Suparmi Suparmi Suparmi Suparmi Suparmi Suparmi Susilawati, Susilawati Syahputri, Erlina Windi tari, Yurina Teddi Seftiadi Azfan Titieu Keumala Sukandar Tjipto Leksono Tjipto Leksono Tjipto Leksono Ulil Amri Vabellina Nuzul Wahyu Oloan Sagala Willy Novfrendy Damanik Windarti Windarti Winona Oktania Abriestina Wisda Samosir P Yoanes Namora Sembiring Yovanie, Faira Yuliana Yuliana Yusuf Eko Syafti