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Physicochemical and Functional Properties of Nano-Chitosan Derived from Green Mussel (Perna viridis) Shells Firmani, Ummul; Safitri, Nur Maulida; Rahim, Andi Rahmad
Journal of Aquaculture and Fish Health Vol. 14 No. 2 (2025): JAFH Vol. 14 No. 2 June 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i2.68319

Abstract

Increasing stocking density in catfish cultivation is part of efforts to increase production to meet catfish needs nationally and internationally. The obstacles faced by household Green mussels (Perna viridis) are a major fishery commodity in Indonesia, producing substantial shell waste. Green mussel shell waste can be used as shrimp feed because of its high calcium content. Nano-chitosan, derived from chitin extracted from green mussel shells, offers enhanced bioavailability and functional properties, making it a promising additive for feed applications. Nano chitosan has the advantage of being biocompatible, biodegradable, and non-toxic. The goal of this study is to create a nano chitosan extract from green mussel shells. Green mussel shells are extracted during the stages of deproteination, demineralization, and deacetylation. The obtained chitosan extract is then combined with Sodium Tripolyphosphate (NaTTP) to yield nano chitosan particles. The nano chitosan particles were then measured and analyzed for the compounds they contained. The extracted chitosan appeared white, with a yield of 71%, which aligns with previous studies on shell-derived chitosan. FTIR analysis confirmed the presence of characteristic chitosan functional groups, including C=O and O-H, at 343 nm. This study successfully synthesized nano-chitosan from green mussel shells that have biocompatibility and high calcium content.
Analisis biometri kepiting bakau (Scylla serrata) yang ditangkap dari hutan mangrove untuk mendukung kegiatan penggemukan kepiting bakau di Kabupaten Gresik Maulana, Andi Moh Roby; Armaansyah, Dimas; Safitri, Nur Maulida; Farikhah

Publisher : Fishery Product Technology Study Program, Yudharta University, Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/lempuk.v2i2.4261

Abstract

This study aims to analyze the biometry of mud crabs caught from mangrove forests to support the provision of kempongan for mud crab cultivators. The research was conducted in Pangkahwetan Village, Ujungpangkah District, Gresik Regency, using a descriptive method. Samples (n= 2,240) collected in the period March-July 2022 from local fishermen. The research variables were biometry, carapace width frequency distribution, condition factor (C), ratio of male and female crabs, and maturity level of mud crabs. Data were analyzed using Microsoft Excel 2013. The sex ratio of crabs caught per month was analyzed by t-test (α=5%). The results of this study obtained a higher male sex ratio (88%) compared to females (12%) in the period March - July, 2022. Mud crab maturity based on the frequency distribution of female crab carapace width was 23.8% (March) , 24.1% (April), 61.5% (June), and 45.8% (July) while male crabs 6.2% (March), 2.9% (April), 6.3% (June ), and 8.2% (July). Male crabs are dominated by thin conditions and female crabs are dominated by fat conditions. The male and female crabs were statistically significantly different. The different conditions between male and female crabs obtained in this study indicate the need for separate management between male and female crabs when used as kempongan in a crab fattening business.
BAKTERI PENYERAP LOGAM TIMBAL (Pb) DARI SEDIMEN DAN KERANG HIJAU, DI LAUT BANYU URIP UJUNG PANGKAH, GRESIK Firmani, Ummul; Safitri, Nur Maulida
Journal of Aquatropica Asia Vol 8 No 1 (2023): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/joaa.v8i1.3769

Abstract

Green mussels (Perna viridis) in Banyu Urip Village, Ujung Pangkah Gresik District, have great potential to be developed due to the abundant availability of seeds in nature, easy cultivation methods, namely using floating net cages and step nets, and high market demand. One of the issues with green mussels is the high content of the heavy metal Lead (Pb) in their flesh, which is caused by heavy metal accumulation in the waters. The goal of this study was to isolate, characterize, and test the ability of heavy metal Lead-Pb bacteria to biosorb heavy metal Pb in post-harvest green mussels from sediments and green mussels in the Banyu urip Ujung Pangkah Gresik Sea. The stages of the research included sampling, isolation, macroscopic and microscopic characterization, in vitro testing of Pb heavy metal absorbent. The results showed 6 bacterial isolates, namely two from green mussels coded K1 and K2 and 4 bacterial isolates from marine sediments coded S1, S2, S3 and S4. The six bacterial isolates obtained macroscopic and microscopic characteristics and have been shown in vitro to be able to grow in Nutrient agar enriched 0.8 mg/L Pb(NO3)2.
Exploring Potential Aquaculture-Immunostimulant-Peptides Derived from Chlorella sorokiniana Safitri, Nur Maulida; Violando, Wiga Alif; Suhermanto, Achmad; Rizkiah, Riza; Mukhaimin, Iman; Ramli, Taufik Hadi; Puspitasari, Asthervina Widyastami; Zummah, Atiqoh
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7585

Abstract

Chlorella sorokiniana is a microalgae with an outstanding nutritional profile and numerous therapeutic substances that can be used as an immunostimulant, including in aquaculture. This research aimed to investigate and characterize peptides isolated from C. sorokiniana protein using TCA digestion and hydrolyzed enzymatically with trypsin. Peptides were then subsequently identified using Tandem LC-MS/MS and Mascot Distiller. Results showed that the percentage of pure protein yield following TCA digestion was 54.66%, and 12 peptides with lengths ranging from 7 to 23 sequences were discovered after trypsin digestion. These peptides originated from various enzymes and chloroplast proteins, including protein synthesis elongation factor TU, photosystem I iron-sulfur center, photosystem II 43 kDa, Ycf4, ATP-dependent zinc metalloprotease FtsH homolog, nitrate reductase, chloroplastic glucose-6-phospate dehydrogenase, and ATP synthase CF1 alpha chain. These findings demonstrated that C. sorokiniana might serve as a source of immunostimulant peptides and proteins, particularly for aquaculture biota.