Claim Missing Document
Check
Articles

Biocontrol Strategies Using Streptomyces spp. and Trichoderma spp. against the Sugarcane Smut Pathogen Sporisorium scitamineum: An in Vitro Study Indrawan, Arga Dwi; Raharjo, Laksamana Agadhia; Rahmadhini, Noni
PLANTA TROPIKA Vol. 14 No. 1 (2026)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v14i1.21414

Abstract

Sporisorium scitamineum is a Basidiomycota pathogen that causes smut disease in sugarcane plants. The pathogen can infect all types of sugarcane, with the extent of damage depending on the plant's susceptibility. Farmers rely on chemical pesticides to control S. scitamineum , but these pesticides have negative environmental impacts. Using Trichoderma sp. and Streptomyces sp. as biocontrol has been proven against fungal infections and is safe for the environment. This study evaluated the potential of Trichoderma sp. and Streptomyces sp. against S. scitamineum under in vitro conditions. Each of the three isolates of Trichoderma sp. and Streptomyces sp. was assessed using the VOC (Volatile Organic Compound), chitinase enzyme, and in vitro antagonistic assays. The results indicated that Streptomyces spp. demonstrated higher chitinase activity compared to Trichoderma spp., with isolate ST1 having the highest chitinase index at 3.7%. VOC test demonstrated that Streptomyces spp. markedly inhibited the mycelial growth of S. scitamineum, attaining inhibition rates of up to 82%. Conversely, Trichoderma spp. exhibited enhanced antagonistic activity via direct interaction, with isolate TD2 suppressing pathogen growth by 82% in dual-culture assays. The findings show that Streptomyces spp. and Trichoderma spp. suppressed S. scitamineum in different but complementary ways. The study shows that they could be used as environmentally friendly biological control agents to manage sugarcane smut.
Screening of quorum quenching activity of rhizobacteria against Pectobacterium carotovorum subsp. carotovorum SAFIRA RIZKA LESTARI; FERY ABDUL CHOLIQ; ANTOK WAHYU SEKTIONO; MOCHAMMAD SYAMSUL HADI; HAIDAR FARI ADITYA; NONI RAHMADHINI; RAMADHANI MAHENDRA KUSUMA; YOGO SETIAWAN
Biodiversitas Journal of Biological Diversity Vol. 23 No. 8 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230859

Abstract

Abstract. Lestari SR, Choliq FA, Sektiono AW, Hadi MS, Aditya HF, Rahmadhini N, Kusuma RM, Setiawam Y. 2022. Screening of quorum quenching activity of rhizobacteria against Pectobacterium carotovorum subsp. carotovorum. Biodiversitas 23: 4336-4342. Quorum sensing (QS) is a mechanism for determining the virulence of the phytopathogenic bacterium Pectobacterium carotovorum. P. carotovorum carries out the QS mechanism with the chemical compound acyl-homoserine lactone (AHL) to signal and carry out its pathogenicity and virulence. The pathogenicity of P. carotovorum can be prevented through quorum quencher (QQ) activity derived from bacteria that degrade AHL compounds by producing AHL-lactonase. In the present investigation, AHL-lactonase-producing bacteria were isolated from the rhizosphere of carrot cultivation in Bumiaji Sub-District, City of Batu, East Java, Indonesia. The isolated bacteria were screened for quorum quenching activity. Out of thirty-seven isolates, 7 rhizospheric bacteria showed QQ activity against the Chromobacterium violaceum bioindicator. The hypersensitivity test on tobacco plants showed that, five strains SRL07, SRL09, SRL15, SRL21, and SRL35 did not cause a hypersensitivity response. The five isolates were able to suppress the virulence of P. carotovorum in carrot tubers. The 16S rRNA gene sequences of the five isolates had the highest similarity to Bacillus thuringiensis, B. subtilis, and B. cereus. The five isolates were detected to have the gene encoding AHL-lactonase (aiiA). The present study provides new information about the QQ activity of rhizosphere and its potential as a quorum quencher against P. carotovorum.
Arthropods discovered on refugio flowering plants in Mangifera indica plantation WIWIN WINDRIYANTI; NONI RAHMADHINI; ITO FERNANDO; RAMADHANI MAHENDRA KUSUMA
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240915

Abstract

Abstract. Windriyanti W, Rahmadhini N, Fernando I, Kusuma RM. 2023. Arthropods discovered on refugio flowering plants in Mangifera indica plantation. Biodiversitas 24: 4747-4754. The diversity of Refugio flowering plants with different ways of flowering phenology provides alternative food sources for arthropods. Insects are the largest class of arthropods, most of which are ecological services that function as natural enemies and pollinators. Insects require an energy source over a long-term period to promote a stable and healthy population. The use of flowering plants as refugia can maximize the role of insects as ecological services in agroecosystems by providing natural enemies and pollinators with a source of food and shelter. The refugia plants used in this study were sunflowers (Helianthus annuus), king’s salad (Cosmos caudatus), Peruvian zinnia (Zinnia peruviana), Tridax procumbens (Asteraceae), and Alternanthera sessilis (Amaranthaceae) with hedgerow and insectary bank planting patterns. Data collection and periodic direct observation (scan sampling) were conducted by transects. Observed parameters included species composition and population of arthropods in two different agro-systems, monoculture and polyculture. Identification results showed 8 orders, including 44 morphospecies, belonging to 25 families. Arthropod abundance in the on-season of the refugia was found in 1422 individuals. Ecological engineering is a habitat manipulation technology using refugia plants that has increased the composition and population of natural enemy insects and pollinators.
Co-Authors Achmad Fiqri Agustin, Eva Tri Aldi Kurniansyah Amir, Faradila Anasta, Dinda Nadia anggreini, riski ayu Anita, Tri Yuli ANTOK WAHYU SEKTIONO Arga Dwi Indrawan Ari Wijayanti Arika Purnawati Arman Adrian Maulana Barna Deta Cristin Naftaly Basroh Saepul Mubarok Cantika, Gabriella Choerunnisa, Agustya Salma Damayanti, Alfa Dirsista Dita Megasari Elly Syafriani Elly Syafriani Erlyana Desy Rahmawati Fatimah Lailatus Saadah FERY ABDUL CHOLIQ Fitri Wijayanti Habibah, Isnaini Ainun HAIDAR FARI ADITYA Haidar Fari Aditya Hasbulloh, Mu'arif Ihza Herry Nirwanto Herry Nirwanto Hery Nirwanto, Hery Imanadi, Latief Indah Nathasya, Rachmawaty Indra Djiwanata ITO FERNANDO Karuniadin Mukti, Ahmad Novarda Kemal Fathirsyam Laksamana Agadhia Raharjo Laksmi Diana Mochammad Mirza Saputra MOCHAMMAD SYAMSUL HADI Muhammad Arif Wahyudin Muhammad Arif Wahyudin Muhammad Qoyyimul Amri Musarofa, Musarofa Okta Gegana Purwadi Penta SURYAMINARSIH Prasetyo, Dandy Pribadi, Didik Utomo Putri, Fanny Etrisya Putri, Redwika Clarissa Putri, Vadila Mulia Raharjo, Laksamana Agadhia RAMADHANI MAHENDRA KUSUMA Ramdan Hidayat Retno Widya Ningrum Risdiyanti, Rateh Lailatul Safira Rizka Lestari Saputra, Mochammad Mirza Saputra, Mochammad Mirza Suharto Suputa Suputa Tito Ridho Nugroho Tri MUJOKO Trijanti A. Widinni Triwahyu, Endang U'ud Uda Marlina Ukhrowi, Syerlina Titis Muawanah Widajati, Wiludjeng Widayati, Wiludjeng Wijayanto, Mohamad Adi Wiludjeng Widayati Windriyanti, Wiwin Wiwik Sri Harijani WIWIN WINDRIYANTI Yenny WURYANDARI YOGO SETIAWAN YUDI NUR SUPRIADI Yulifada, Dede Angelina Zetta Rasullia Kamandang