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EKSPLORASI DAN IDENTIFIKASI NEMATODA ENTOMOPATOGEN (Steinernema spp. dan Heterorhabditis spp.) DI DATARAN TINGGI DLUNDUNG KABUPATEN MOJOKERTO PADA KERAPATAN VEGETASI YANG BERBEDA: EXPLORATION AND IDENTIFICATION OF ENTOMOPATHOGENIC NEMATODES (Steinernema spp. and Heterorhabditis spp.) IN THE DLUNDUNG HIGHLAND OF MOJOKERTO DISTRICT AT DIFFERENT VEGETATION DENSITIES Habibah, Isnaini Ainun; Rahmadhini, Noni; Kusuma, Ramadhani Mahendra
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 13 No. 4 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2025.013.4.7

Abstract

Nematoda entomopatogen (NEP) merupakan agen pengendali hayati yang efektif dalam mengelola hama pertanian karena mampu membunuh inangnya secara cepat dan ramah lingkungan. Penelitian ini bertujuan untuk mengeksplorasi dan mengidentifikasi nematoda entomopatogen (NEP) di dataran tinggi Dlundung, Trawas, Mojokerto, serta menganalisis hubungan antara kerapatan vegetasi dan kepadatan populasinya. Pengambilan sampel dilakukan pada dua lokasi berbeda ketinggian, yaitu 980 mdpl (vegetasi padat, NDVI 0,55) dan 1020 mdpl (vegetasi jarang, NDVI 0,23), dengan metode soil baiting menggunakan larva Tenebrio molitor sebagai umpan. Hasil isolasi menunjukkan adanya dua genus NEP, yaitu Steinernema spp. dan Heterorhabditis spp., yang dibedakan berdasarkan ciri morfologi dan warna kutikula inang terinfeksi. Populasi NEP lebih tinggi pada vegetasi padat (29,51 JI/mL) dibandingkan vegetasi jarang (15,86 JI/mL), meskipun perbedaan tidak signifikan (p > 0,05). Temuan ini menunjukkan bahwa kedua lokasi memiliki kondisi lingkungan yang sesuai bagi perkembangan NEP dan berpotensi menjadi sumber isolat lokal untuk pengendalian hayati hama.
Distribution Pattern and Population Density of Spodoptera frugiperda (Lepidoptera: Noctuidae) in Corn Fields in the Tanggunggunung Hills, Tulungagung Regency Nugroho, Tito Ridho; Windriyanti, Wiwin; Rahmadhini, Noni
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8376

Abstract

Background: Corn is an important commodity in Tulungagung Regency; however, infestations of Spodoptera frugiperda have caused substantial yield losses, particularly during the vegetative growth stage, with reported damage levels reaching 20–70% depending on infestation intensity. Understanding the population density and spatial distribution of this pest under local agroecosystem conditions is therefore essential for developing effective control strategies. This study aimed to analyze the population density, distribution pattern, and environmental relationships of S. frugiperda on corn plants in the hilly area of Tanggunggunung, Tulungagung Regency. Methodology: The research was conducted on corn plants aged 15 to 45 days post-planting at two distinct locations: Ngrejo Village (approximately 110 meters above sea level) and Jengglungharjo Village (approximately 145 meters above sea level), each encompassing an area of 25 square meters. A two-dimensional sampling method was employed, utilizing 10 fixed observation plots per field, with observations occurring every three days. The variables observed included pest population density, symptoms of pest attack, and abiotic environmental factors such as temperature and humidity. Distribution patterns were analyzed using the Morisita index and the degree of Morisita (Ip), while the relationships between pest populations and environmental factors were examined through Pearson’s correlation analysis. Findings: The population of S. frugiperda in Ngrejo village exhibited a random distribution pattern (Id= 1.016; Ip= 0.004), whereas in Jengglungharjo village, it demonstrated a tendency towards clustering (Id = 0.211; Ip= 0.456). The density of the pest population showed a strong positive correlation with temperature (r= 0.799 to 0.702) and a negative correlation with humidity (r= −0.661 to −0.423). Contribution: Microclimatic conditions play a key role in shaping the spatial distribution and population density of S. frugiperda, highlighting the importance of site-specific, ecology-based pest management to improve corn productivity in hilly agroecosystems
The Effect of Insect Flour on The Growth Media of Beauveria bassiana in Controlling the Pupa Stage of Bactrocera carambolae Choerunnisa, Agustya Salma; Rahmadhini, Noni; Windriyanti, Wiwin
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/17713

Abstract

Background: One effective method for controlling Bactrocera carambolae is the use of Beauveria bassiana. However, a common challenge is the decline in the conidial quality and virulence due to the lack of chitin and protein sources. The insect Tenebrio mollitor and the pupal casings of Hermetia illucens are rich in chitin and have the potential to enhance the growth medium. Methods: The study used RAKF consisting of 3 factors and 3 replications. The first factor was the Type of Flour (T) consisting of T1: T. mollitor and T2: H. illucens. The second factor was Flour Concentration (K), which consisted of K1: 0.5% K2: 1% and K3: 1.5%. The third factor was Drying Temperature (S), of S1: 1000C S2: 1100C and S3: 1200C. Results: The results of the study show that the treatment caused symptoms of infection in the form of mycelium growth on the pupal cuticle and abnormalities in the emergence imago. The best combination is T2K2S2 (1% H. illucens flour at 1100C) which gives a mortality rate up to 76.7%, pupal duration of 9.3 days, and viability rate of 92.2%. Conclusions: The combination of H. illucens flour at a concentration of 1% and a drying temperature of 1100C is quite effective in increasing the ability of B. bassiana in controlling B. carambolae pupae in vitro.
Production and Characterization of Nanoparticles from Citronella (Cymbopogon nardus) Extract Based on Chitosan Compounds Damayanti, Alfa Dirsista; Rahmadhini, Noni; Kusuma, Ramadhani Mahendra
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18769

Abstract

Background: The use of nanoparticles in Indonesia has been developing and is now being applied across sectors, including agriculture, health, and industry. Nanoparticles derived from Cymbopogon nardus can be used as nanopesticides because they contain several compounds that suppress pathogen growth and reduce pest populations. Chitosan is an additional compound used in the fabrication of these nanoparticles. Methods: This research used materials and tools, including a PSA (Particle Size Analyzer), a SEM (Scanning Electron Microscope), Cymbopogon nardus, and chitosan. The research stages included extraction of Cymbopogon nardus, nanoparticle fabrication, PSA analysis, zeta potential measurement, and SEM observation. Results: The PSA test revealed that the Cymbopogon nardus nanoparticles had an average size of 907.2 nm. The zeta potential of the nanoparticles was –40.62 mV, indicating good electrostatic stability. SEM images showed that the nanoparticles had an irregular shape. Conclusions: The Cymbopogon nardus nanoparticles produced in this study, as characterized by PSA, zeta potential, and SEM analyses, demonstrated the expected characteristics of nanoparticles.