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The Effect of Insect Flour on The Growth Media of Beauveria bassiana in Controlling the Pupa Stage of Bactrocera carambolae Choerunnisa, Agustya Salma; Rahmadhini, Noni; Windriyanti, Wiwin
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/17713

Abstract

Background: One effective method for controlling Bactrocera carambolae is the use of Beauveria bassiana. However, a common challenge is the decline in the conidial quality and virulence due to the lack of chitin and protein sources. The insect Tenebrio mollitor and the pupal casings of Hermetia illucens are rich in chitin and have the potential to enhance the growth medium. Methods: The study used RAKF consisting of 3 factors and 3 replications. The first factor was the Type of Flour (T) consisting of T1: T. mollitor and T2: H. illucens. The second factor was Flour Concentration (K), which consisted of K1: 0.5% K2: 1% and K3: 1.5%. The third factor was Drying Temperature (S), of S1: 1000C S2: 1100C and S3: 1200C. Results: The results of the study show that the treatment caused symptoms of infection in the form of mycelium growth on the pupal cuticle and abnormalities in the emergence imago. The best combination is T2K2S2 (1% H. illucens flour at 1100C) which gives a mortality rate up to 76.7%, pupal duration of 9.3 days, and viability rate of 92.2%. Conclusions: The combination of H. illucens flour at a concentration of 1% and a drying temperature of 1100C is quite effective in increasing the ability of B. bassiana in controlling B. carambolae pupae in vitro.
Production and Characterization of Nanoparticles from Citronella (Cymbopogon nardus) Extract Based on Chitosan Compounds Damayanti, Alfa Dirsista; Rahmadhini, Noni; Kusuma, Ramadhani Mahendra
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18769

Abstract

Background: The use of nanoparticles in Indonesia has been developing and is now being applied across sectors, including agriculture, health, and industry. Nanoparticles derived from Cymbopogon nardus can be used as nanopesticides because they contain several compounds that suppress pathogen growth and reduce pest populations. Chitosan is an additional compound used in the fabrication of these nanoparticles. Methods: This research used materials and tools, including a PSA (Particle Size Analyzer), a SEM (Scanning Electron Microscope), Cymbopogon nardus, and chitosan. The research stages included extraction of Cymbopogon nardus, nanoparticle fabrication, PSA analysis, zeta potential measurement, and SEM observation. Results: The PSA test revealed that the Cymbopogon nardus nanoparticles had an average size of 907.2 nm. The zeta potential of the nanoparticles was –40.62 mV, indicating good electrostatic stability. SEM images showed that the nanoparticles had an irregular shape. Conclusions: The Cymbopogon nardus nanoparticles produced in this study, as characterized by PSA, zeta potential, and SEM analyses, demonstrated the expected characteristics of nanoparticles.
The Potential for Controlling Maize Pest Spodoptera frugiperda (Lepidoptera: Noctuidae) Using Biological Agents Bacillus spp. Muhammad Qoyyimul Amri; Yenny Wuryandari; Noni Rahmadhini
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 15 No. 2 (2026): April 2026
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v15i2.608-617

Abstract

Maize production is still facing many challenges nowadays.  One of the main challenges in maize production is pest attacks.  S. frugiperda has been causing significant damage to maize farming in Indonesia recently.  This research was conducted to determine the potential of Bacillus spp. isolates Bcz-20 and Bcz-30 are identified as biological agent candidates to control the S. frugiperda population, with bacterial population densities of 109 CFU/mL, 108 CFU/mL, and 107 CFU/mL, respectively, as determined by in vitro and in vivo application tests.  The Bcz-20 treatment with a population density of 107 CFU/mL was capable of suppressing the S. frugiperda larvae population in an in vitro application test, resulting in a mortality percentage of 47.5%. The Bcz-20 treatment with a population density of 109 CFU/mL was capable of suppressing the S. frugiperda larvae population by in vivo application test, resulting in a mortality percentage of 70 %.  The conclusion, Bacillus spp. Bcz-20 isolates with a population density of 107 CFU/mL are efficient and effective for controlling the S. frugiperda larvae population.
Studi Pola Persebaran Nematoda Entomopatogen di Lahan Tebu Kecamatan Plosoklaten Kabupaten Kediri Arman Adrian Maulana; Ramadhani Mahendra Kusuma; Noni Rahmadhini
Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Vol 21 No 1 (2026): Mei 2026
Publisher : Universitas Islam Balitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35457/dx5wh934

Abstract

Pemanfaatan NEP sebagai APH memiliki beberapa keunggulan karena dapat bertahan di dalam tanah tanpa adanya inang. Terdapat 30 famili nematoda yang menjadi parasit bagi invertebrata dan tujuh diantaranya berpotensi sebagai NEP seperti Mermithidae, Allantonematidae, Neotylenchidae, Sphaerularidae, Rhabditidae, Steinernematidae, dan Heterorhabditidae. Kediri memiliki potensi yang cukup besar sebagai sumber isolat NEP mengingat kondisi agroekosistem yang memungkinkan keberadaan NEP dengan luas lahan pertanian sebesar 82.884 ha setara dengan 64% total luas lahan. Penelitian ini bertujuan mempelajari pola persebaran kerapatan NEP pada lahan tebu berdasarkan titik pengambilan sampel. Lahan dengan luas 600 m2 dibagi menjadi 5 titik sampel secara menyilang. Pengambilan sampel tanah asal 5 titik sampel dibagi menjadi 10 titik sub-sampel dengan pola bintang. Isolasi dilakukan dengan metode baiting dan white trap, sedangkan perhitungan kerapatan dilakukan sebanyak 3 kali pada setiap ulangan. Analisis statitik menggunakan pengujian Kruskal-Wallis dilanjutkan dengan visualisasi menggunakan ArcGIS. Kerapatan terendah berada pada titik 1 dan 2, meningkat sedikit pada titik 4, lalu meningkat kembali di titik 5 dan paling tinggi terletak di titik 3. Secara visual, agregasi NEP di lapangan mengikuti pola distribusi terpusat. Perbedaan kerapatan ini dapat disebabkan oleh berbagai faktor seperti intensitas penyinaran yang berpengaruh terhadap suhu dan kelembaban tanah.
Fluctuations in Fruit Fly Population (Bactrocera spp.) and Its Relationship to Environmental Conditions in Red Guava Plantations in Sidoarjo Regency Indra Djiwanata; Noni Rahmadhini; Herry Nirwanto
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 8 No. 1 (2026): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 8 No. 1 January 2026
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v8i1.4706

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Crystal guava (Psidium guajava) is a superior variety cultivated in Indonesia. A major constraint in crystal guava production is the high incidence of pest attacks, particularly from fruit flies. This study was conducted in a red guava orchard located in Sidoarjo Regency and Jalan Simorejosari A IV/17 during the period from July to August. The research employed a survey method involving the installation of attractant traps and purposive sampling to collect infested fruit for host rearing. Both trapping and host rearing were used to monitor the fruit fly population. Trapping was carried out by installing attractant traps at five predetermined plots, positioned at a height of 1.5 meters and spaced 20 meters apart. Fruit fly rearing involved collecting infested fruit using purposive sampling from the same five plots, then incubating until adult flies emerged. The fruit fly species attacking the red guava orchard was identified as Bactrocera dorsalis. Independent t-test analysis revealed significant differences between the populations of trapped fruit flies and those obtained from host rearing. The emergence rate of fruit fly adults from host rearing exhibited a negative correlation with the trapped fruit fly population, described by the regression equation Y = 359.282 – 7.2146X. Path analysis indicated that rainfall positively correlates with temperature and humidity, while temperature negatively correlates with humidity. Additionally, rainfall and humidity negatively correlate with the trapped fruit fly population, whereas temperature shows a positive correlation.
Distribution Pattern and Population Density of Spodoptera frugiperda (Lepidoptera: Noctuidae) in Corn Fields in the Tanggunggunung Hills, Tulungagung Regency Tito Ridho Nugroho; Wiwin Windriyanti; Noni Rahmadhini
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8376

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Background: Corn is an important commodity in Tulungagung Regency; however, infestations of Spodoptera frugiperda have caused substantial yield losses, particularly during the vegetative growth stage, with reported damage levels reaching 20–70% depending on infestation intensity. Understanding the population density and spatial distribution of this pest under local agroecosystem conditions is therefore essential for developing effective control strategies. This study aimed to analyze the population density, distribution pattern, and environmental relationships of S. frugiperda on corn plants in the hilly area of Tanggunggunung, Tulungagung Regency. Methodology: The research was conducted on corn plants aged 15 to 45 days post-planting at two distinct locations: Ngrejo Village (approximately 110 meters above sea level) and Jengglungharjo Village (approximately 145 meters above sea level), each encompassing an area of 25 square meters. A two-dimensional sampling method was employed, utilizing 10 fixed observation plots per field, with observations occurring every three days. The variables observed included pest population density, symptoms of pest attack, and abiotic environmental factors such as temperature and humidity. Distribution patterns were analyzed using the Morisita index and the degree of Morisita (Ip), while the relationships between pest populations and environmental factors were examined through Pearson’s correlation analysis. Findings: The population of S. frugiperda in Ngrejo village exhibited a random distribution pattern (Id= 1.016; Ip= 0.004), whereas in Jengglungharjo village, it demonstrated a tendency towards clustering (Id = 0.211; Ip= 0.456). The density of the pest population showed a strong positive correlation with temperature (r= 0.799 to 0.702) and a negative correlation with humidity (r= −0.661 to −0.423). Contribution: Microclimatic conditions play a key role in shaping the spatial distribution and population density of S. frugiperda, highlighting the importance of site-specific, ecology-based pest management to improve corn productivity in hilly agroecosystems
Comparative Analysis of Insect Pest Diversity in Cocoa (Theobroma cacao L.) Agroecosystems under Different Shade Tree Canopies: Papaya vs. Banana Basroh Saepul Mubarok; Ramadhani Mahendra Kusuma; Noni Rahmadhini
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20289

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This study compared the pest insect community structure in cocoa plantations shaded by papaya and banana trees. The study was conducted from January to March 2025 using systematic sampling in six cocoa fields, consisting of three papaya-shaded and three banana-shaded plantations. In each field, 19 traps were installed, comprising nine yellow traps, nine pitfall traps, and one light trap, supported by visual observations. Pest insects were identified to the genus level, and community structure was assessed using diversity (H′), evenness (E′), richness (R′), and dominance (C′) indices. Banana-shaded cocoa fields supported slightly more pest insect genera than papaya-shaded fields, with 22 and 21 genera recorded, respectively. Banana-shaded plots also showed higher diversity, evenness, and richness, accompanied by lower dominance. These patterns indicate that the denser banana canopy may create more heterogeneous microhabitat conditions that support a broader range of pest insects. Shade tree type influences pest insect community structure in cocoa plantations. Banana shade was associated with a more diverse and balanced pest insect community, whereas papaya shade showed relatively higher dominance, suggesting a greater risk of specific pest outbreaks. These findings highlight the importance of shade tree selection in integrated pest management, particularly for designing site-specific monitoring and targeted control strategies in cocoa plantations.
Pengaruh Cahaya dan Wadah Penyimpanan terhadap Efektivitas Nuclear Polyhedrosis Virus dalam Mengendalikan Spodoptera litura Anasta, Dinda Nadia; Rahmadhini, Noni
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/20321

Abstract

Spodoptera litura pest attacks can cause disturbances in plant growth and lead to considerable yield losses. Control that is often done to control S. litura is usually with chemical insecticides, but excessive use of chemical insecticides has the potential to cause adverse effects on the environment. As a safer and more sustainable alternative, pest control can be done through the utilization of biological agents such as pathogens that function as bioinsecticides, one of which is Nuclear Polyhedrosis Virus (NPV). This research aims to analyze the effect of length of exposure to sunlight and the type of storage container on the effectiveness of NPV in controlling S. litura. NPV isolates were exposed to direct sunlight for 1, 3, 6 hours in glass and plastic vial containers. The isolate was infected to 3rd instar S. litura larvae using feed contamination technique. This research implemented a factorial Completely Randomized Design (CRD), with 6 treatments and control. The results showed that NPV isolates with MK.1 and MP.1 treatments were effective in producing a percentage of larvae mortality and were able to suppress the formation of pupae and imago with a mortality percentage of 84,2 % and 71,4 % at 72 hours after aplication. Length of sunlight exposure affects the effectiveness of NPV isolates.
Biocontrol Strategies Using Streptomyces spp. and Trichoderma spp. against the Sugarcane Smut Pathogen Sporisorium scitamineum: An in Vitro Study Indrawan, Arga Dwi; Raharjo, Laksamana Agadhia; Rahmadhini, Noni
PLANTA TROPIKA Vol. 14 No. 1 (2026)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v14i1.21414

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Sporisorium scitamineum is a Basidiomycota pathogen that causes smut disease in sugarcane plants. The pathogen can infect all types of sugarcane, with the extent of damage depending on the plant's susceptibility. Farmers rely on chemical pesticides to control S. scitamineum , but these pesticides have negative environmental impacts. Using Trichoderma sp. and Streptomyces sp. as biocontrol has been proven against fungal infections and is safe for the environment. This study evaluated the potential of Trichoderma sp. and Streptomyces sp. against S. scitamineum under in vitro conditions. Each of the three isolates of Trichoderma sp. and Streptomyces sp. was assessed using the VOC (Volatile Organic Compound), chitinase enzyme, and in vitro antagonistic assays. The results indicated that Streptomyces spp. demonstrated higher chitinase activity compared to Trichoderma spp., with isolate ST1 having the highest chitinase index at 3.7%. VOC test demonstrated that Streptomyces spp. markedly inhibited the mycelial growth of S. scitamineum, attaining inhibition rates of up to 82%. Conversely, Trichoderma spp. exhibited enhanced antagonistic activity via direct interaction, with isolate TD2 suppressing pathogen growth by 82% in dual-culture assays. The findings show that Streptomyces spp. and Trichoderma spp. suppressed S. scitamineum in different but complementary ways. The study shows that they could be used as environmentally friendly biological control agents to manage sugarcane smut.
Co-Authors Achmad Fiqri Agustin, Eva Tri Aldi Kurniansyah Amir, Faradila Anasta, Dinda Nadia anggreini, riski ayu Anita, Tri Yuli Arga Dwi Indrawan Ari Wijayanti Arika Purnawati Arika Purnawati Arman Adrian Maulana Barna Deta Cristin Naftaly Basroh Saepul Mubarok Cantika, Gabriella Choerunnisa, Agustya Salma Damayanti, Alfa Dirsista Didik Utomo Pribadi Didik Utomo Pribadi Dita Megasari Elly Syafriani Elly Syafriani Erlyana Desy Rahmawati Fatimah Lailatus Saadah Fitri Wijayanti Habibah, Isnaini Ainun Haidar Fari Aditya Hasbulloh, Mu'arif Ihza Herry Nirwanto Herry Nirwanto Hery Nirwanto, Hery Imanadi, Latief Indah Nathasya, Rachmawaty Indra Djiwanata Indrawan, Arga Dwi Karuniadin Mukti, Ahmad Novarda Laksamana Agadhia Raharjo Laksmi Diana Lestari, Safira Rizka Mochammad Mirza Saputra Muhammad Arif Wahyudin Muhammad Arif Wahyudin Muhammad Qoyyimul Amri Musarofa, Musarofa Okta Gegana Purwadi Penta SURYAMINARSIH Prasetyo, Dandy Putri, Fanny Etrisya Putri, Redwika Clarissa Putri, Vadila Mulia Raharjo, Laksamana Agadhia Ramadhani Mahendra Kusuma Ramadhani Mahendra Kusuma Ramdan Hidayat Retno Widya Ningrum Risdiyanti, Rateh Lailatul Safira Rizka Lestari Saputra, Mochammad Mirza Saputra, Mochammad Mirza Suharto Tito Ridho Nugroho Tri MUJOKO Trijanti A. Widinni Triwahyu, Endang Ukhrowi, Syerlina Titis Muawanah Widajati, Wiludjeng Widayati, Wiludjeng Wijayanto, Mohamad Adi Wiludjeng Widayati Windriyanti, Wiwin Wiwik Sri Harijani Wiwin Windriyanti Wiwin Windriyanti Wiwin Windriyanti Yenny WURYANDARI YUDI NUR SUPRIADI Yulifada, Dede Angelina Zetta Rasullia Kamandang