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Journal : Natural Science: Journal of Science and Technology

PENENTUAN NILAI SPF (SUN PROTECTING FACTOR) EKSTRAK N-HEKSAN ETANOL DARI RICE BRAN (ORYZA SATIVA) SECARA IN VITRO DENGAN METODE SPEKTROFOTOMETRI UV-VIS Mulyani, Mulyani; Syamsidi, Armini; Putri, Pramita
Natural Science: Journal of Science and Technology Vol 4, No 1 (2015): Volume 4 Number 1 (March 2015)
Publisher : Univ. Tadulako

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Abstract

Sinar matahari merupakan sumber energy bagi kelangsungan hidup semua makhluk di bumi, namun pada paparan berlebih di kulit akan memberikan derajat kerusakan yang tergantung pada frekuensi dan lamanya sinar matahari mengenai kulit. Kemampuan menahan sinar ultraviolet dari sediaan tabir surya dinilai sebagai factor proteksi sinar (Sun Protecting Factor/SPF). Penelitian ini bertujuan untuk menentukan SPF (Sun Protection Factor) dari ekstrak n-Heksan etanol bekatul (Oryza sativa) dengan menggunakan metode spektrofotometri. Bekatul diekstraksi dengan metode soxhlethasi menggunakan larutan penyari n-Heksanetanol, kemudian dibuat seri konsentrasi larutan sampel 100 ppm, 150 ppm, 200 ppm, 250 ppm, dan 300 ppm dengan pelarut etanol absolut. Larutan tersebut selanjutnya diukur absorbansinya pada spektrofotometer UV-Vis dengan panjang gelombang 280-400 nm. Hasil penelitian menunjukkan bahwa ekstrak n-Heksanetanol bekatul dapat efektif memberikan perlindungan pada sinar UV-B. Nilai SPF yang diperoleh mulai dari konsentrasi 200 ppm, dengan nilai SPF sebesar 2,355, selanjutnya pada konsentrasi 250 ppm sebesar2,884 dan pada konsentrasi 300 ppm sebesar 3,483.
PENGARUH VARIASI EKSTRAK METANOL KULIT BUAH RAMBUTAN (NEPHELIUM LAPPACEUM L.) TERHADAP KESTABILAN FISIK KRIM ANTIOKSIDAN Syamsidi, Armini
Natural Science: Journal of Science and Technology Vol 3, No 2 (2014): Volume 3 Number 2 (August 2014)
Publisher : Univ. Tadulako

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Abstract

ABSTRACT A research on influence methanol extracts concentration of fruit peel rambutan (N. lappaceum L.), concerning physical stability of creams antioxidant had been done. This research aims to obtain an antioxidant cream formulation O/W type from rambutan fruit peel extracts and compares activity of the antioxidant cream with extract from fruit peel rambutan with using free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. A total of 500 grams of fruit rambutan peel extracted with 80% methanol by maceration method and then extract obtained made antioxidant cream O/W type with the variation of the concentration of extract 1%, 2%, and 3% to the total weight of the cream. Evaluation of physical stability of creams includes organoleptic testing, creaming, viscosity testing and drop size of dispersed phase inversion before and after the storage conditions during the 12-hour accelerated alternately at 5°C and as many as 10 cycles of 35°C. Organoleptic observations show no change in color and odor on the third-cream formula. The research was used a completely randomized design and the data obtained were analyzed statistically distinguished by ansira on the confidence level. Statistical analysis shows that the concentration of rambutan fruit peel extracts give significant effect on the viscosity cream before and after accelerated storage conditions, while the size of the drops dispersed showed no significant effect. In this study, showed that no phase of inversion and creaming from all of cream. Formulation of skin creams with methanol extract of the fruit rambutan 1% cream can be categorized as the most stable, continuing antioxidant activity test showed that the cream with a concentration of extract 12,359 ppm, which inhibits 50% of free radicals.