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Sumaryati Syukur
Universitas Andalas
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SKRINING DAN IDENTIFIKASI BAKTERI TERMOFILIK PENGHASIL SELULASE DAN AMILASE DARI SUMBER AIR PANAS BUKIT KILI SOLOK SUMATERA BARAT DENGAN ANALISIS GEN 16S rRNA Zona Octarya; Sumaryati Syukur; Endang Purwati RN
Sistem Informasi Vol 2 No 1 (2011): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v2i1.125

Abstract

Enzim termostabil dari bakteri termofilik merupakan enzim yang sangat potensial untuk mengatasi kendala teknis industri yang berhubungan dengan proses pada suhu tinggi. Salah satu sumber enzim adalah mikroorganisme termofilik yang banyak terdapat pada sumber air panas. Indonesia merupakan negara kepulauan yang banyak mempunyai sumber air panas, salah satunya adalah Kabupaten Solok. Penelitian ini mencakup skrining dan identifikasi bakteri penghasil enzim termostabil selulase dan amilase dari sumber air panas Bukit Kili Ketek di Kabupaten Solok. Suhu air panas adalah 52°C pada bulan Juni 2009 dan pH air adalah 8. Kultur murni yang diperoleh adalah sebanyak 22 kultur Dari hasil pewarnaan gram, diperoleh sebagian besar bakteri air panas adalah jenis bakteri streptobacilli (basilus) gram positif, hanya dua isolat yang merupakan gram negatif yaitu isolat S1B dan S5B. S2A adalah isolat yang mempunyai aktifitas selulase paling tinggi dibandingkan dari isolat yang lain karena mempunyai indeks zona bening selulase lebih tinggi yaitu 2,6. Isolat S2A juga menghasilkan amilase tinggi dengan indeks zona bening 2,5. Persen identitas bakteri air panas S2A yang diperoleh adalah 97% dengan Anoxybacillus flavithermus strain AE3.
The Antibacterial Activity Test Comparison of Green and Black Tea Ethanol Extract (Camellia sinensis) Against Propionibacterium acnes Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
Science Midwifery Vol 10 No 2 (2022): April: Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v10i2.544

Abstract

A study was conducted in connection with the comparison of antibacterial activity tests between ethanol extracts from green tea leaves and black tea leaves (Camellia sinensis) against Propionibacterium acnes. There were obtained by a softening technique that was softened for 24 hours. The filtrate was then concentrated to give a concentrated extract and evaporated ethanol content. The concentrated extract was then diluted with distilled water to extract 5%, 10%, and 15% concentration variations. The test method for inhibitory activity was the disc method using distilled water as a negative blank. The results obtained with blank, 5%, 10% and 15% green tea were 0. 5.25; 7.05 and 7.95 mm and black tea ethanol extract were 0; 2.50; 3.20 and 5.30 mm. The conclusion of this study is that the concentration of the extract is directly proportional to its inhibitory power, and the green tea ethanol extract is more potent than the black tea ethanol extract, so both can be used as antibacterial agents against P. acnes.
Potensi Bakteri Asam Laktat Sebagai Kandidat Probiotik Antimikroba Patogen Asal Fermentasi Kakao Varietas Criollo Urnemi Urnemi; Sumaryati Syukur; Endang Purwati; Sanusi Ibrahim; Jamsari Jamsari
Jurnal Riset Teknologi Industri Vol 6 No 12 Desember 2012
Publisher : Balai Riset dan Standardisasi Industri Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8019.535 KB) | DOI: 10.26578/jrti.v6i12.1519

Abstract

Lactic Acid Bacteria (LAB) were isolated from cocoa beans three days fermentation Criollo variety. That were eleven isolates, all of isolates were named Gr3 (1, 2, 3, 4,…,11). All of isolates were tested to antimicrobial activity against indicator pathogenic bacteria (Escherichia coli  14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, Listeria monocytogenesis dan Salmonella typhii),  than that was chosen potential one isolate had highest inhibition zones to pathogens.  One potential isolate was done determination weigh protein bacteriocin by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Result of antimicrobial activity test showed that, 11 isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 11 mm at 48 hours observation. That was found one isolate potential, it was Gr3.7.  Gr3.7 isolate had high inhibition zones to against growth pathogenic bacteria, that was 11mm to Salmonella typhii and Listeria monocytogenesis at 48 hours observation. Determination crude weight protein test was estimated 24 kDa protein by using SDS-PAGE.  To Found purifying weight protein bacteriocin must be done purification processing. Probiotic candidate (Gr3.7) can be used as natural antibiotics, because it had positive responce to against Salmonella typhii.  Salmonella typhii was known pathogen in intestine,  beside that it was used as food preservative, because it had positive response to against Listeria monocytogenesis.   
The Inhibitory Activity Test of Green Tea Ethanol Extract (Camellia sinensis) Sidamanik Against Escherichia coli Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Khaidir
Science Midwifery Vol 10 No 2 (2022): April: Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A study was done in analyzing the inhibitory activity of green tea ethanol extracts against Escherichia coli. The green tea is sourced from a tea plantation in Sidamanik, North Sumatra. The green tea was macerated with ethanol 96 % for 24 hours. After that, the filtrate was taken and then concentrated to give a concentrated extract and evaporated ethanol content until run out and obtained thick extract. The thick extract was then diluted with distilled water to extract 5%, 10%, and 15% concentration variations. The test method for inhibitory activity was the disc method using distilled water as a negative blank. The results obtained with blank, 5%, 10% and 15% green tea were 0. 5.7; 7.10, and 8.05 mm The conclusion of this study is that the concentration of the extract is directly proportional to its inhibitory power, and can be used as an antibacterial agent against E. coli.
GREEN TEA ETHANOL EXTRACT EFFICACY AGAINST PSEUDOMONAS AERUGINOSA Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 9, No 1 (2022): August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i1.7336

Abstract

Research about green tea ethanol extract efficacy against Pseudomonas aeruginosa has been done. The green tea leaves were sourced from tea distributor in Sidamanik District, North Sumatra, Indonesia. Green tea leaves extract was obtained by maceration technique in which green tea leaves were soaked with ethanol 96% for 24 hours. After that, the filtrate was concentrated to be thick extract with no liquid content again.  After that, the concentrated extract was made by 5%, 10% and 15% as extract variations. The efficacy of green tea ethanol extract against Pseudomonas aeruginosa  was signed by increasing the inhibitory diameter that using disc method, in which the distilled water was used as negative blank. The results obtained for blanks, 5%, 10% and 15% extracts were 0; 1.85; 2.9 and 4.45 mm. The conclusion of this study is that the concentration of the extract is increasing proportionally to its inhibitory power, the higher of extract concentration is higher of its inhibitory activity against Pseudomonas aeruginosa. The conclusion described the green tea ethanol extract was effective to be antibacterial agent against Pseudomonas aeruginosa.
Isolasi dan Karakterisasi Bakteri Asam Laktat dari Teh Hijau Fermentasi Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
Lumbung Farmasi: Jurnal Ilmu Kefarmasian Vol 3, No 2 (2022): Juli
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/lf.v3i2.9645

Abstract

ABSTRAKTeh hijau adalah jenis teh yang tidak mengalami proses fermentasi akan tetapi mengalami proses pengeringan dan penguapan daun. Teh hijau memiliki aktivitas antioksidan yang tinggi karena kandungan katekin di dalamnya. Namun bioavaibilitas katekin teh hijau berkurang pada saluran cerna karena kurang stabil. Kehadiran probiotik pada teh hijau diharapkan mampu meningkatkan bioavaibilitasnya. Bakteri Asam Laktat (BAL) merupakan kelompok bakteri baik yang merupakan probiotik yang berperan dalam menyehatkan saluran cerna. Peneltian ini bertujuan untuk mengisolasi dan menguji karakterisasi BAL dari Teh Hijau Fermentasi. Teh hijau yang berasal dari Distributor Teh Juma, Kecamatan Sidamanik difermentasi dengan gula aren dan madu hutan dengan variasi waktu 24, 48 dan 72 jam pada suhu rendah. Teh hijau fermentasi kemudian diisolasi kemudian diencerkan dengan pengenceran 10-5 dan 10-6. Hasil inkubasi selama 48 jam diperoleh kemudian dimurnikan dengan metode streak dan diinkubasi kembali selama 24 jam kemudian diuji karakterisasinya secara morfologi, pewarnaan gram, uji katalase dan uji fermentasi. Hasil yang diperoleh adalah pada uji morfologi positif BAL dengan ciri-ciri terlihat koloni tunggal dengan bentuk bulat, licin, warna putih kekuningan.dan terbentuk zona bening di sekitar koloni. Hasil pewarnaan positif BAL dengan ciri-ciri bakteri gram positif ditunjukkkan oleh warna biru ke ungu. Hasil uji katalase ditunjukkan dengan ciri-ciri  tidak menghasilkan gelembung udara yang menandakan adanya BAL karena BAL tidak mengandung oksigen serta uji fermentasi yang ditunjukkan dengan tidak terbentuknya gelembung udara pada tabung Durham yang menandakan tipe fermentasi BAL adalah homofermentatif. Kata kunci : Isolasi; Karakterisasi; Bakteri asam laktat; Teh hijau; Fermentasi.ABSTRACTGreen tea is a type of tea that does not undergo a fermentation process but undergoes a process of drying and evaporation of the leaves. Green tea has high antioxidant activity due to the catechin content in it. However, the bioavailability of green tea catechins is reduced in the gastrointestinal tract because it is less stable. The presence of probiotics in green tea is expected to increase its bioavailability. Lactic Acid Bacteria (LAB) is a group of good bacteria which are probiotics that play a role in healthy gastrointestinal tract. This study aims to isolate and test the characterization of LAB from Fermented Green Tea. Green tea from Juma Tea Distributor, Sidamanik District was fermented with palm sugar and forest honey with variations of 24, 48 and 72 hours at low temperature. The fermented green tea was isolated and then diluted with 10-5 and 10-6 dilutions. The results of incubation for 48 hours were obtained and then purified by streak method and incubated again for 24 hours and then tested for morphological characterization, gram staining, catalase test and fermentation test. The results obtained were a positive morphological test of LAB with the characteristics of a single colony being seen with a round, smooth, yellowish white color, and a clear zone was formed around the colony. The results of positive LAB staining with gram-positive bacteria are indicated by a blue to purple color. The results of the catalase test are indicated by the characteristics of not producing air bubbles which indicate the presence of LAB because LAB does not contain oxygen and the fermentation test is indicated by the absence of air bubbles in the Durham tube which indicates the type of LAB fermentation is homofermentative. Keywords : Isolation; Characterization; Lactic acid bacteria; Green tea; Fermentation;
GREEN TEA ETHANOL EXTRACT EFFICACY AGAINST PSEUDOMONAS AERUGINOSA Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 9 No. 1 (2022): August 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i1.7336

Abstract

Research about green tea ethanol extract efficacy against Pseudomonas aeruginosa has been done. The green tea leaves were sourced from tea distributor in Sidamanik District, North Sumatra, Indonesia. Green tea leaves extract was obtained by maceration technique in which green tea leaves were soaked with ethanol 96% for 24 hours. After that, the filtrate was concentrated to be thick extract with no liquid content again.  After that, the concentrated extract was made by 5%, 10% and 15% as extract variations. The efficacy of green tea ethanol extract against Pseudomonas aeruginosa  was signed by increasing the inhibitory diameter that using disc method, in which the distilled water was used as negative blank. The results obtained for blanks, 5%, 10% and 15% extracts were 0; 1.85; 2.9 and 4.45 mm. The conclusion of this study is that the concentration of the extract is increasing proportionally to its inhibitory power, the higher of extract concentration is higher of its inhibitory activity against Pseudomonas aeruginosa. The conclusion described the green tea ethanol extract was effective to be antibacterial agent against Pseudomonas aeruginosa.
STUDY OF ANTI-OXIDANT ACTIVITY TEST ON NATURAL MATERIALS USING 1,1-DIFENYL-2-PIKRILHIDRAZIL (DPPH) Syukri Arief; Risma Sari; Novesar Jamarun; Sumaryati Syukur
JURNAL ILMIAH TEKNOLOGI INDSUTRI (SAINTI) Vol 16, No 2 (2019): VOL 16 NO 2 DESEMBER 2019
Publisher : Politeknik ATI Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.45 KB)

Abstract

This study aims to determine the anti-oxidant potential found in natural materials. The antioxidant activity test was carried out on several natural ingredients or common plants that have been known and used for generations by the people of Indonesia. Antioxidant activity testing was carried out using DPPH method using IC50 parameters and natural material samples used were virgin coconut oil from coconut plants, Tanjung leaves and bay leaves. Antioxidant activity test showed that the sample of natural materials used had strong antioxidant activity, it was found that IC50 values were between 50-100 ppm.
Pelatihan Praktikum Kimia Sederhana di SMAN 16 Padang Imelda Imelda; Refinel Refinel; Refilda Refilda; Yefrida Yefrida; Hermansyah Aziz; Yulizar Yusuf; Suryati Suryati; Zulkarnain Chaidir; Sumaryati Syukur; Rahmiana Zein; Novesar Jamarun; Safni Safni; Zilfa Zilfa; Yetria Rilda; Norman Ferdinal; Tommi Hermansyah; Fivi Mona Bareno
Warta Pengabdian Andalas Vol 30 No 3 (2023)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.30.3.562-568.2023

Abstract

Chemistry is one of the subjects taught in State Senior High Schools (SMAN), especially in Natural Sciences (IPA). The problem with chemistry subjects is that it is difficult to practice in the laboratory because laboratory facilities such as space, equipment and chemicals are expensive. As a result, chemical laboratory practices were only sometimes carried out. Several lecturers and students from the Department of Chemistry, FMIPA Unand Kimia Unand, carried out service activities at SMAN 16 Padang through laboratory practice using materials and equipment that were cheap and readily available in the surrounding environment. This community service aims to train teachers and students to carry out chemistry practicums using simple materials and equipment available around them. The training was given to chemistry subject teachers and students in the science laboratory at SMAN 16 on 17 and 21 November 2022. The material was practised in oxidation and reduction (redox) and electrolysis reactions. The activity went smoothly; the students enthusiastically carried out the practicum. Through the results of this simple chemistry practicum, students can design and carry out chemical experiments using simple tools and materials, conduct direct observations of chemical processes, practice scientific thinking, attitude and work skills and analyze and solve various scientific processes using scientific methods. SMAN 16 hopes that this activity will continue with different topics.
Identıfıcatıon of KRAS Mutatıons in Colorectal Carcınoma Patıents at Dr. M. Djamıl Hospıtal, West Sumatra-Indonesıa Rita Maliza; Hevi Horiza; Sumaryati Syukur; Allimuddin Tofrizal; Bramadi Arya
Medical Laboratory Technology Journal Vol. 9 No. 2 (2023): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v9i2.535

Abstract

Kirsten rat sarcoma viral oncogene (KRAS) gene mutations lead to abnormal activation of the RAS signalling pathway and have been associated with poor prognosis and resistance to some therapeutics. This study aimed to identify mutation characteristics of the KRAS genes codon 12 and 13 in colorectal cancer patients in West Sumatra. KRAS mutations were analyzed in 20 DNA of colorectal cancer patients' tissue samples by using polymerase chain reaction (PCR) with specific primer and direct sequencing analysis. Our findings showed five samples (25%) with mutated KRAS at codons 12 and 13 (including three samples with GGT→GAT, one sample with GGT→GTT in codon 12, and one sample with GGC→GAC in codon 13). In conclusion, we found two variations of amino acid changes at codon 12 (G12D and G12V) and one at codon 13 (G13D). More research with many samples is required to obtain conclusive data on the relationship between these gene mutations and colorectal cancer response to therapy and prognosis.
Co-Authors - Evitayani -, Risfaheri -, Urnemi -, Vovien -, Wiranda Abdi Dharma Allimuddin Tofrizal Armaini - Azhar, Minda Azheri, Busra Bramadi Arya Budiman, Cipta Dahlyanti, Rina Desmiwarti Desmiwarti DESSY NATALIA Endang Purwati RN Endang Syamsudin fahmi, Aliyah - Femilya Putri Ferdinal Ferry Fivi Mona Bareno Helmi Arifin Hermansyah Aziz Hermansyah Aziz Hevi Horiza Hidayat, Zoni Imelda Imelda Jamsari Jamsari Lidya Sari Utami Lili Warly Maliza, Rita Masrial Masrial Melia, Sri Melia, Sri Minda Azhar Murni, Yulia Nanda Tri Wahdini Natalia, Dessy Norman Ferdinal Novesar Jamarun Novesar Jamarun Putri Zelfitri Zen Rahmi Nofita Rahmiana Zein Refilda Refilda Refinel - Rina Dahlyanti Risfaheri - Risma Sari Safni Safni Sanusi Ibrahim Sartika, Diza Sumanti, Titi Suryati Suryati Syukri Arief Tio Putra Wendari Titi Sumanti Tommi Hermansyah Tri Astuti Upita Septiani Urnemi - Urnemi Urnemi Vovien - Wardi, Epi Supri Wiranda - Yanwirasti Yanwirasti Yefrida Yefrida Yetria Rilda Yulia Murni Yulizar Yusuf Zilfa Zilfa ZK Abdurahman Baizal Zona Octarya Zona Octarya Zoni Hidayat Zozy Aneloi Noli Zulkarnain Chaidir Zulkarnain Khaidir