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Exploration and Quality Assessment of Local Trichoderma spp. from the Rhizosphere of Bamboo (Gigantochloa apus) Against Fusarium Wilt Disease in Tomato Plants Pulle, Yosevin H.; Tanjung, Rosye H.R.; Lantang , Daniel
Jurnal Biologi Papua Vol 17 No 1 (2025)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jbp.4190

Abstract

This study aims to explore and test the quality of Trichoderma spp. local from the rhizosphere of apus bamboo (Gigantochloa apus) against Fusarium spp. wilt disease both in vitro and in vivo in tomato. This research was conducted in the Keerom area, Jayapura, which is one of the centers of food crop production and horticulture in Papua. The data obtained from this study were analyzed by comparing the inhibition of three Trichoderma spp. isolates (Trichoderma sp. 1 isolate, Trichoderma sp. 2 isolate, and Trichoderma sp.3 isolate) against Fusarium spp. The results of the antagonist test showed that the isolate of Trichoderma sp.3 had the highest inhibition (80.56%) against Fusarium spp., followed by Trichoderma sp.2 (66.84%) and Trichoderma sp.1 (27,62%). The implication of this study is that Trichoderma sp. 3 has high potential as an APH in the control of Fusarium spp. wilt disease in tomato plants. The use of Trichoderma spp.-based APH can be an effective and environmentally friendly alternative in reducing the use of chemical pesticides and maintaining the balance of the agro-environmental ecosystem.
Pathogenic Fungi Causing Disease on Merbau Plants (Intsia spp.) in Sawesuma Village Forest, Urunum Guay District, Jayapura Regency Cahyaningsih, Ayu; Tanjung, Rosye H.R.; Runtuboi, Dirk Y.P.
Jurnal Biologi Papua Vol 17 No 1 (2025)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jbp.4515

Abstract

Plant diseases result from the interaction of virulent pathogens, susceptible host plants, and favorable environmental conditions, often indicated by the appearance of growth abnormalities, referred to as symptoms, across various plant parts.This study aims to identify the species of Intsia plants affected by diseases and to determine the types of fungi responsible for causing diseases in Intsia sp. This research involved field surveys, observations, and sampling of pathogenic fungi, followed by fungal isolation and identification at the Mycology Laboratory and the FMIPA Biology Laboratory, Cenderawasih University, Jayapura. The methods employed included site surveys, sampling, isolation of pathogenic fungi causing diseases in Intsia sp. , identification of disease-causing fungi, and pathogenicity testing. Based on observations, the Intsia species affected by diseases were Intsia bijuga and Intsia palembanica. Four fungal species were identified as disease-causing agents in Intsia plants, including Aspergillus sp., Penicillium sp., Fusarium sp., and Mucor sp., along with one unidentified fungal species.
DNA Barcoding of Cacao Pod Rot Fungi in Papua Province Gunaedi, Tri; Tanjung, Rosye H.R.; Manwan, Sri Wahyuni
East Asian Journal of Multidisciplinary Research Vol. 3 No. 2 (2024): February 2024
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/eajmr.v3i2.8294

Abstract

Cocoa (Theobroma cacao) pod rot fungi  have various types. In general, cause cocoa pod rot  are fungi of the species Fusarium sp, Rhizoctonia sp, Phytium sp, Colletotricum sp, Amerosporium sp, Phomopsis sp and Phytopthora sp. Various species of fungi that can rot cocoa pods have high similarity in morphology characters even though the treatment for each species of fungi is different. This problem occurs in the people's nucleus cocoa plantations in the Yapsi district, Jayapura, Papua province. Samples of cacao pods infected with the fungus were taken and the isolates obtained were reinoculated into uninfected cacao pods. Isolates showing dominant infectivity were taken and identified molecularly. Identification was carried out by creating DNA barcoding by sequencing the Internal Transcribed Spacer (ITS) region of the 18SrDNA marker gene from the fungal isolates SP4DYAPSI-1, SP4 DYAPSI-2, SP3DYAPSI-1 and SP6DAUN3. Sequencing data in the form of isolate fungi DNA barcodes were analyzed with the ClustalX program analyses. The resulting phylogenetic tree construction shows that the four isolates are related to the fungus species Fusarium solani with a nucleotide similarity index above 95%. This shows that the cause of damage to cocoa pods in the Yapsi area, Jayapura, Papua, Indonesia is the fungus Fusarium solani.
Co-Authors Agamawan, Lalu Panji I. Agnes Kabelen Agnes Kabelen, Agnes Akrom, Akrom Alfred A Antoh Ali Djamhuri Alianto , Baigo Hamuna Baigo Hamuna Baigo Hamuna Baigo Hamuna Baigo Hamuna Basa T Rumahorbo Cahyaningsih, Ayu Daniel Lantang Dewi Sulistiowati Dwi Haryanto Dwi Haryanto Edy Meiyanto Efray Wanimbo Elsye Gunawan Eni Miton Busup Erni Unenor Erni Unenor, Erni Ervina Indrayani Fali Sembori Fali Sembori Floriana Kabelen Hanna E. Samosir Helena G Burok Helena G. Burok Henderina J. Keiluhu Henderina J. Keiluhu Hendra Kurniawan Maury Ign. Joko Suyono Irma A. Resmol Irma Rahayu Irma Rahayu, Irma Jane T. Sada Jane T. Sada Jhon D. Kalor Jhon D. Kalor John D Kalor Juwita Juwita Kabes, Wetty Queenisa Kalvin Paiki Komari Komari Konstantina M.B. Kameubun Konstantina M.B. Kameubun Kristopholus Rumbiak Lantang , Daniel Lisiard Dimara Lisye I. Zebua Lisye I. Zebua Lisye I. Zebua Lisye Iriana Zebua Lisye Iriana Zebua Lucia Runggeari Lucia Runggeari Mahmudi Mahmudi Maklon Warpur Manalu, Khristhoper Aris A. Manwan, Sri Wahyuni Marcelino N. Yonas Marthen L.R. Bartholomeus Mathen L.R. Bartholomeus Meidy J. Imbiri Mesak Kamarea Mesak Kamarea Mesak Kamarea Nelince Fakdawer Nurtanti HAndono Nurtanti Handono Parjan Parjan Pulle, Yosevin H. Purwanto Purwanto Purwanto Purwanto R. Rijanta Rejauw, Korinus Rini Patandungan Rini Patandungan Runtuboi, Dirk Yanes Saebu, Yusuf Sammaria J. Karma Septriyanto Dirgantara Sipriana Dogomo Siregar, Martha D. Suharno Suharno Suharno Suharno Suharno Suharno Suharno Suharno Suharno, _ Supeni Sufaati Supeni Sufaati Supeni Sufaati Supeni Sufaati Supeni Sufaati Supeni Sufaati Suriani B.R. Surbakti Suriani Br. Surbakti Suwito Suwito Suwito Suwito Suwito Suwito Totok A. Mushid Tri Gunaedi Verena Agustini Verena Agustini Verena Agustini Verena Agustini Wahyu Dwi Nugroho Wigati Yektiningtyas Wigati Yektiningtyas Yan P. Yepese Yan Piet Yepese Yayan Sopian Yemeus Helakombo Yuliana Ruth Yabansabra Yuliana Yuliana Yulius Sarungu Yunus Pajanjan Paulangan Ziyadatun Ni'mah