, Triadiati
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Agathis, Kampus IPB Darmaga, Bogor, 16680, Indonesia

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Androgynomonoecious Jatropha curcas: Chromosomes, Isozymes, and Flowers Gender Triadiati Triadiati; Kurniati Kurniati; Utut Widyastuti; Dasumiati Dasumiati
HAYATI Journal of Biosciences Vol. 26 No. 3 (2019): July 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.606 KB) | DOI: 10.4308/hjb.26.3.139

Abstract

Jatropha curcas (J. curcas) is usually monoecious plants, which have male and female flowers on the same inflorescence. However, J. curcas can be found as an androgynomonoecious plant (have male, female, and hermaphrodite flowers), even though very rare. Androgynomonoecious J. curcas can be identified after six months of planting when it had started flowering. Therefore, it is important to identify the characteristics of androgynomonoecious J. curcas that can differentiate between androgynomonoecious and monoecious plants in earlier stages of growth. The objectives of the research were to observe isozymes, chromosome and flowers gender of androgynomonoecious and monoecious J. curcas Banten and Lampung accessions. Seeds from five genotypes of J. curcas were used in the research. The observation was carried out on the chromosome and isozymes (Peroxidase and Esterase isozymes) could be used as markers to differentiate androgynomonoecious and monoecious plants. Observations about the flower gender from offsprings derived from different seeds were important to know the inheritance of flower gender. The androgynomonoecious and monoecious J. curcas were diploid with number of chromosomes 2n=2x=22. The chromosomes of androgynomonoecious have longer than that of monoecious J. curcas. The isozymes of androgynomonoecious J. curcas had four alleles and monoecious J. curcas (Banten female monoecious) had three alleles. The flower inflorescence and gender derived from androgynomonoecious plants were unstable, due to androgynomonoecious is intermediate state.
Pertumbuhan dan Produksi Padi Sawah dan Gogo dengan Pemberian Pupuk Hayati Berbasis Bakteri Pemacu Tumbuh di Tanah Masam Arie Aryanto; . Triadiati; . Sugiyanta
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 3 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1172.919 KB) | DOI: 10.18343/jipi.20.3.229

Abstract

Biofertilizer is a substance which contains microorganism and promotes plant growth by increasing the soil nutrient availability. The aim of this study was to analyze the growth and production of lowland and upland rice by using the biofertilizer in acid soils. Seven isolates of bacteria were used as biofertilizer, i.e. Bacillus sp., two strains of Pseudomonas sp., two strains of Azospirillum sp., and two strains of Azotobacter sp.. The treatments of this study were without fertilization, compost, 100% NPK, compost enriched with 7 isolates, compost enriched with 7 isolates + 50% NPK, compost enriched with 4 isolates, and compost enriched with 4 isolates + 50% NPK. The results showed that biofertilizer in compost improved nutrient content in acid soil, and increased rice growth and production as well. Compost enriched with 7 isolates 50% NPK showed the highest rice yield production both in the lowland and upland rice. The biofertilizer could reduced 50% utilization NPK fertilizer dosage in the first growing season.
Pertumbuhan dan Produksi Rimpang Kencur (Kaempferia galanga L.) pada Ketinggian Tempat yang Berbeda Subaryanti Subaryanti; Yohana Caecilia Sulistyaningsih; Dyah Iswantini; Triadiati Triadiati
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.012 KB) | DOI: 10.18343/jipi.25.2.167

Abstract

Galanga (Kaempferia galanga L.) is one of the potential medicinal plants with high demand. Therefore, galanga cultivation was still quite promising. To obtain the optimum growth and high rhizome production, the superior galanga accessions are required. The optimal growth and high production of galanga accessions will be obtained if cultivated at the area with appropriate altitude. The purpose of the study was to obtain galanga accession with a better growth and high yield at the area with a suitable altitude. This study used split-plot design; altitude as a main plot and accession as a subplot. The location as a main plot consisted of altitudes of 214 and 780 masl. This study used seven accessions i.e., accession of PBG (Purbalingga), CLP (Cilacap), PWJ (Purworejo), KRA (Karanganyar), PCT (Pacitan), MAD (Madiun), and GAL2 (Galesia 2) as control. The results showed that different altitudes significantly affected the total chlorophyll content, stomatal conductance, transpiration rate, photosynthesis rate, dry weight, and rhizome yield. The number of leaves was influenced by altitude and accession of galanga. The interaction between altitude and accession of galanga significantly affected the leaf area and the soluble sugar concentration in the galanga rhizome. The rhizome yield at low altitude was higher than in high altitude. PBG (Purbalingga) and PWJ (Purworejo) accessions have the potential to be developed in locations with an altitude of 214 masl. Keywords: altitude, Kaempferia galanga, rhizome
Pertumbuhan, Produksi, dan Kualitas Buah Melon dengan Pemberian Pupuk Silika Triadiati Triadiati; Mafrikhul Muttaqin; Nelly Saidah Amalia
Jurnal Ilmu Pertanian Indonesia Vol. 24 No. 4 (2019): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.502 KB) | DOI: 10.18343/jipi.24.4.366

Abstract

Melon (Cucumis melo L.) is a plant that has economic value and has been cultivated in several areas of Indonesia with intensive fertilization. The mineral nutrient that is expected to improve the quality of melon growth and fruit is silica. The aim of the research was to improve the growth and melon fruit quality by addition of silica fertilizer. The treatments in this experiment were the application of silica (Novelgro, water soluble) which was carried out on melon plants with silica concentrations of 0.67; 1.33 ppm; and control (without silica). The results showed that silica increased the plant height, leaf area, and diameter of melon fruit. Melon plant with silica treatment of 1.33 ppm had silica content in the high category (>4% Si) which was 5.31% in the skin of the fruit. Moreover, the addition of silica can prolong the fruit storage period in room temperature compared to control. 
Analisis Morfofisiologi, Anatomi, dan Histokimia pada Lima Spesies Tanaman Gulma sebagai Respons terhadap Merkuri dan Timbal Rani Apriyani Raharja; Hamim Hamim; Yohana Caecilia Sulistyaningsih; Triadiati Triadiati
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 3 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.3.412

Abstract

Plants can be used as phytoremediation agents to improve critical land due to gold mining activities. This experiment aimed to analyze the morphophysiological, anatomical, and histochemical responses of Brachiaria mutica, Cyperus kyllingia, Ipomea aquatica, Mikania micrantha, and Paspalum conjugatum in response to the application of heavy metals mercury and lead in the forms of Hg(NO3)2 and Pb(NO3)2 in hydroponic experiments and to determine the ability of these plants to accumulate both metals. Morphological responses were observed by measuring number of leaves and plant dry weight, and physiological responses were observed by analyzing photosynthetic pigments, while anatomical and histochemical responses were analyzed by microscopic observation to tranversal slice of roots and leaves. The results showed that the applications of Hg(NO3)2 and Pb(NO3)2 treatments caused decreases in number of leaves, plant dry weights, and photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoid). The treatments also decreased leaf thickness due to the decrease in the epidermis, but they caused the increases in exodermis and endodermis of the roots. Mercury and lead were accumulated in large amounts in the roots, but accumulation in the shoot was less. Histochemical observation showed that lead was found in the roots of all the plants, especially in endodermic tissue and the vessel, whereas in the leaves the two metals were detected in the upper and lower epidermis, mesophyll, and vessel. Among the five species tested, C. kyllingia and P. conjugatum were the most tolerant to Pb and Hg.
The Physiological Responses of Zea Mays L. and Cucumis Sativus L. on Drought Stress and Re-Watering Selis Meriem; Evi Muliyah; Melisnawati H. Angio; Triadiati Triadiati
Jurnal Biodjati Vol 6, No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.12572

Abstract

Drought leads to deficit water availability and its detrimental effects seriously threaten plant growth. This study assessed the physiological, biochemical, and antioxidant adjustments in different types of photosynthetic plants between Zea mays L. (C4) and Cucumis sativus L. (C3 plant) under response to short-term drought stress. Analyses of relative water content (RWC), proline, and ascorbic acid (AsA) were performed to explore how these plants react to drought. Fifteen-day-old plants were subjected to full irrigation or gradual drought periods for 2-d, 4-d, 6-d, and 8-d following by recovery for 7-d. The results revealed that drought significantly reduces leaf RCW in both plants. Re-watered Z. mays after 8-d drought was higher than C. sativus and reestablished RCW by 23% of stressed plant although remained lower by 9% of the well-watered plant. While, proline and AsA contents in Z. mays were higher than those in C. sativus in drought treatment at 8-d (2.05 µmol/g FW) and 6-d (3174.60 AsA/100 g FW), respectively, that could demonstrate osmotic adjustment ability in this C4 species. The increased proline in both plants also indicates a good strategy for plants to recover. Rewatering gave a decrease AsA and could be expected that plants restore cellular activity after oxidative injury. Based on our study, proline is the most informative biochemical marker to differentiate plant response to drought and Z. mays adjusted defense mechanism to drought rather than C. sativus due to higher accumulation of proline, better antioxidant activity, and improved RCW after recovery.
Determining Phytocomponent of Vetiver Grass (Chrysopogon zizanioides) Under Drought Stress Ani Sulistiyani; Syamsul Falah; Triadiati Triadiati
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.11686

Abstract

Vetiver grass (Chrysopogon zizanioides, Poaceae) is the leading commodity in Garut Regency, Indonesia, the second-largest producer in the world of vetiver oils. The content of vetiver oils is strongly influenced by the environment, for example, drought stress. Drought stress causes plants to adapt by producing secondary metabolites such as essential oils. This experiment aimed to analyze growth, phytocomponents and obtaining the best quality of vetiver grass accessions under drought stress. The results showed that root and shoot dry biomass were significantly affected by the interaction between drought stress duration and vetiver grass accession. The root dry biomass of Kamojang accession decreased by 25.4%, while Cilawu increased by 5% for 4 days of drought stress. The root length and shoot length were not significantly affected by the treatment. The highest root/shoot length ratio was Verina, and the lowest one was Cisarua. The highest increase in proline occurred in Cilawu accession (85.7%), while the lowest was Verina (6.67%). Essential root oils contain 53 types of phytocomponents, dominated by sesquiterpenes, being khusimol, the highest type. The Cilawu is the best accession based on growth and content rendement.
Keragaan Morfo-Fisiologi Phaseolus spp. yang Ditanam Pada Ketinggian Tempat yang Berbeda (Morpho-Physiological Performance Phaseolus spp. on Different Altitudes) Delfi Trisnawati; nFN Triadiati; Nisa Rachmania Mubarik
Jurnal Hortikultura Vol 28, No 1 (2018): Juni 2018
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v28n1.2018.p59-66

Abstract

Permintaan masyarakat di Indonesia terhadap tanaman legumes meningkat setiap tahunnya, namun produksi legumes di Indonesia belum dapat mencukupi kebutuhan konsumen. Pertumbuhan tanaman legume dipengaruhi oleh faktor lingkungan seperti ketinggian tempat, suhu, intensitas cahaya matahari, dan kelembapan. Tujuan penelitian ini untuk menganalisis dan mengevaluasi keragaan morfo-fisiologi Phaseolus spp. yang ditanam pada dua ketinggian tempat yang berbeda. Empat jenis legumes yang digunakan adalah Jackson Wonder Bean (JWB - Phaseolus lunatus), Christmas Bean (CHB - P. lunatus), Cranberry Bean (CRB - P. vulgaris), dan White Kidney Bean (WKB - P. vulgaris). Empat macam kacang tersebut ditanam pada ketinggian tempat 1.100 m dpl. (lokasi I) dan pada ketinggian 250 m dpl. (lokasi II). Peubah fisiologi dipengaruhi oleh lokasi penelitian, sedangkan peubah morfologi dipengaruhi oleh interaksi antara ketinggian tempat dan jenis kacang. Perbedaan kondisi lingkungan memengaruhi habitus JWB (P. vulgaris). CHB (P. lunatus) menghasilkan jumlah polong tertinggi pada ketinggian tempat 1.100 m dpl., yaitu 25,7 polong diikuti dengan BB 100 biji, yaitu 158,3 g. Implikasi penelitian ini adalah pembudidayaan tanaman legumes atau kacang minor pada dua lokasi yang berbeda berdasarkan ketinggian tempat untuk mendapatkan kondisi lingkungan yang sesuai agar pertumbuhan dan produksinya optimal.KeywordsPhaseolus lunatus; Phaseolus vulgaris; Bintil akar; Konduktansi stomata; Laju fotosintesisAbstractThe demand of legumes in Indonesia is increasing every year. The growth performance of legumes is influenced by environmental factors such as altitude land, temperature, light intensity, and air humidity. The aims of this study were to analyze and evaluate the morpho-physiological performance of legumes (Phaseolus spp.) grown in two different altitudes. Four genotypes of bean used in this study were Jackson Wonder Bean (JWB - P. lunatus), Christmas Bean (CHB - P. lunatus), Cranberry Bean (CRB - P. vulgaris), and White Kidney Bean (WKB - P. vulgaris). The four species of beans were grown at 1,100 m asl. (location I) and 250 m asl. (location II). The physiological parameters were influenced by location, meanwhile the morphological parameter was affected by the interaction between the altitude and type of beans. The differences on environmental condition in both field affected the habits of WKB (P. vulgaris). The CHB (P. lunatus) bean produced the highest number of pods at altitude of 1,100 m asl followed by wet weight of 100 seeds is 25.7 and 158.3. The implication of this research is the cultivation of beans minor at two different locations based on the altitude to get the appropriate environmental conditions to be optimal growth and production.
Characterization of Gibberellin Producing Rhizobacteria Isolated from Soil Forest in Banten Hadi Susilo; Nisa Rachmania Mubarik; Triadiati Triadiati
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Gibberellin is plant growth regulator that stimulates cell elongation, seed germination, flowering, and fruit ripening. This study was conducted to isolate, identify, and optimize growth media for gibberellins producing- rhizobacteria isolated from rhizosphere soil of “keruing“ (Dipterocarpus sp.) tree in forest research Carita, Pandeglang, Banten. Eight bacterial isolates were obtained and all produced gibberellin. The BC2 isolate produced the highest of gibberellin (0.897 mg mL-1) and then selected for identification based on physiology, molecular character, and effects of growth media with variation of temperature, pH, and light. The result of physiological test indicated that BC2 isolate does not produce indole, positive on urease and oxidative carbohydrate. The phylogenetic analysis showed that BC2 isolate is belonged to Stenotrophomonas maltophilia with 98% similarity level. The optimation of growth media indicated that the growth of BC2 isolate was optimum at 30°C, pH 7, and dark condition.
Pemberdayaan Masyarakat pada Budi Daya dan Pengembangan Produk Pohon Gaharu (Aquilaria sp.) di Kabupaten Tolitoli, Sulawesi Tengah Triadiati Triadiati; Miftahudin Miftahudin
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 7 No. 2 (2021): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.7.2.174-184

Abstract

Tolitoli District has a potential for agarwood trees in the forests, but it has not been optimally utilized and preserved by the local communities. Therefore, efforts are needed to increase understanding and community involvement in cultivation and product development of agarwood trees. The objectives of this activity are: to explore agarwood tree species in the forest and sources of local inoculums using a purposive sampling method for agarwood production, to assess the suitability of agro-climates for agarwood tree cultivation, to conduct face-to-face training and practice for agarwood tree breeding and bio-induction by injection, and to identify socio-economic conditions to support agarwood tree cultivation for community empowerment through product development and cultivation in Kabupaten Tolitoli. The project was implementated by exploring and identifying the existence of natural agarwood trees and agarwood farmers, training, and mentoring. The results of exploration and identification showed that Kabupaten Tolitoli has natural resources of agarwood trees in the forest and local inoculums for the bio-induction of agarwood formation. Also, the local community, including the local government, practitioners, farmers, and educational institutions, have great interest and desire for product development and cultivation of agarwood trees. Thus, it can be concluded that community empowerment through product development and cultivation of agarwood trees in Kabupaten Tolitoli can be implemented.