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Journal : Jurnal Ilmiah Berkala: Sains dan Terapan Kimia

Design of Vaccine Candidate Based on Ebola Virus Epitop With In Silico Approach Erwin Prasetya Toepak; Sari Namarito Simarmata; Sudarman Rahman; Stevin Carolius Angga
Jurnal Sains dan Terapan Kimia Vol 16, No 1 (2022)
Publisher : Program Studi Kimia, Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (720.028 KB) | DOI: 10.20527/jstk.v16i1.12167

Abstract

ABSTRACT          The World Health Organization (WHO) recorded as many as 2,299 cases of death with a case percentage of 66% during 2018 to 2020 in the Democratic Republic of the Congo due to the Ebola virus. Ebola virus is a member of the Filoviridae family. One of the viro cores consists of glycoprotein (GP). Messenger RNA (mRNA) generates long GP chains for the attachment protein (GP 1) and the fusion protein (GP 2). Epitope-based vaccines are a promising approach because epitopes represent immunogenic regions that elicit immunity specifically. Epitope prediction was performed based on the GP EBOV sequence available in the Data Bank. The designed vaccine could be one of the candidates for the Ebola virus vaccine. The design of the virus with access to NCBI AAB81004 was carried out by testing such as B cell epitope, T cell, and their antigenicity using the VaxiJen v2.0 server and IEDB. The T cell epitope prediction results showed that 20 T cell epitopes interacted with the Major Histocompatibility Complex (MHC) with the highest score of 2.8069. B cell epitope by linear BepiPred assay had 77 candidate epitope peptides from sequence 401-477. Karplus and Schulz's flexibility predictions showed a predictive value of 1.119 with a threshold of 1.008, with the analyzed area having an antigenic tendency where the threshold area was yellow. Keywords: Ebolavirus; Vaccine; Epitope B Cell; Epitope T Cell; In Silico
Co-Authors Agung Samudra Agus Lasono Al-Hadi, Risfa Aliya Alayya Adistia Putri Alfanaar, Rockiy Alfanaar, Rokiy Ansori Rahman Arfan Arfan Arsana, Mu afa Arsana, Mu’afa Purwa Arsana, Mu’afa Purwa Atilla Dian Dewanti Gintoro Awalul Fatiqin Ayu, Regina Wahyudyah Sonata Citrariana, Shesanthi Citrariana, Shesanti Defilia Anogra Riani Desimaria Panjaitan Desindria, Galang Rizdho Dewi, Apriana Dita Monita Dwi Hermayantiningsih Elon Jipasca Erwin Prasetya Toepak Erwin Prasetya Toepak Erwin Prasetya Toepak Erwin Prasetya Toepak Erwin Prasetya Toepak Fatiqin, Awalul Febri Nur Ngazizah Ferry Patrix Gerung, Jenny Blessia Gunawan, Yohanes Edy Haq, Muhammad Hasanul I Nyoman, Sudyana, Idin Sahidin Ika Oktavia Wulandari Karyadi Karyadi Karyadi Karyadi Mariasa, Joice Cleriska Mistryani, Mistryani Monita, Dita Muhamad Taufik Hidayat Muhammad Rifqi Shodri Muhammad Takdir Bachtiar Mu’afa Purwa Arsana Mu’afa Purwa Arsana Mu’afa Purwa Arsana Ngazizah, Febri Nur Norhusna, Norhusna Nur Hidayat Parametta, Vidya Putri Wineni Rahman, Ansori Reny Rosalina Riana, Shesanti Citra Rokiy Alfanaar Ruslin Ruslin Sabarudin Sabarudin Sahari, Yudhi Ekakristian Sahidin Sahidin Sahumena, Muhamad Handoyo Sametri, Ni Wayan Septia Sandi Mahesa Yudhantara Saputra, Mika Adi Sari Namarito Simamarta Sari Namarito Simarmata Sari, Meiyanti Ratna Kumala Shesanti Citra Riana Shesanti Citra Riana Shodri, Muhammad Rifqi Simajuntak, Ruth Riama Siti Sunariyati Stevin Carolius Angga Sulistia, Tiya Thathit Suprayogi Yahya Febrianto Yahya Febrianto Yehezkiel Steven Kurniawan Yohanes Edi Gunawan Yosaeunike, Vitatani Ysrafil Ysrafil Ysrafil Ysrafil Yudhi Ekakristian Sahari Yulita, Elia Zahrotun Nafisah Zahrotun Nafisah Zimon Pereiz