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All Journal Jurnal Ilmu Dasar e-Journal Pustaka Kesehatan Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Indonesian Journal of Cancer Chemoprevention Journal of Agromedicine and Medical Sciences Pharmacon Dental Journal (Majalah Kedokteran Gigi) Warta Pengabdian Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi JSFK (Jurnal Sains Farmasi & Klinis) JPSCR : Journal of Pharmaceutical Science and Clinical Research JURNAL AGRIBISAINS PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Ilmu Kefarmasian Indonesia Abdimasku : Jurnal Pengabdian Masyarakat Jurnal Media Teknologi Pharmaceutical Sciences and Research (PSR) Journal of Artificial Intelligence and Digital Business Jurnal Industrial Galuh Journal of Agropharmacy Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)
Endah Puspitasari
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Journal : e-Journal Pustaka Kesehatan

 1 
Uji Aktivitas Antibakteri dan Antibiofilm Minyak Atsiri Rimpang Bengle (Zingiber purpureum Roscoe) terhadap Bakteri Staphylococcus epidermidis (Antibacterial and Antibiofilm Activity Test of Bengle Rhizome Essential Oil (Zingiber purpureum Roscoe) agains Hidayatul Ulyah; Evi Umayah Ulfa; Endah Puspitasari
Pustaka Kesehatan Vol 3 No 2 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Staphylococcus epidermidis has become one of the most important pathogenic bacteria that lead to nosocomial infection. This is caused by its ability to form a biofilm on the surface. Essential oils are one of the natural product that could be used as antiinfection agents. Bengle rhizome essential oil has been known to have activity against non-pathogenic bacteria S. epidermidis. This study was proposed to know the antibacterial and antibiofilm activity of bengle rhizome essential oil against clinical isolate of S. epidermidis at concentrations of 45, 60, 75, and 90%. Antibacterial activity was determined by the diffusion method with hole plate technique, while antibiofilm activity was determined by the microtiter assay method with crystal violet staining and quantified by measuring the optical density (OD) at λ 595 nm. This study showed that bengle rhizome essential oil has antibacterial and antibiofilm activity. The largest antibacterial and antibiofilm activity was shown from 90% concentration of bengle rhizome essential oil. Keywords: nosocomial infections, antibacterial, antibiofilm, bengle essential oil, Staphylococcus epidermidis.
Uji Aktivitas Antimalaria Ekstrak Kering DaunTithonia diversifolia pada Mencit yang Diinfeksi Plasmodium berghei (Antimalarial Activity of Dry Extract of Tithonia diversifolia Leaves on Plasmodium berghei Infected Mice) Novan Eko Setiyanggono; Nuri .; Endah Puspitasari
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Malaria is an infectious disease caused by Plasmodium sp. One of the herbs that are empirically used as an antimalarial is Tithonia diversifolia. Preparation of dried leaf extract of T. diversifolia used oven drying and the addition of cab-o-sil as a dryer. This study aims to determine the antimalarial activity of extracts of dried leaves of T. diversifolia. Antimalarial activity assay method used was a modified peter test. The probit analysis of this study showed that ED50 value on mice given the dry extract of T. diversifolia leaves was 214 mg /kg body weight and categorized as good at classifying antiplasmodium in vivo.     Keywords: antimalarial activity, Tithonia diversifolia, dry extract, ED50
Gambaran Histopatologi Organ Jantung Tikus Putih Jantan Galur Wistar (Rattus norvegicus) dalam Uji Toksisitas Akut Kombinasi Ekstrak Daun Jati Belanda (Guazuma ulmifolia Lmk.) dan Kelopak Bunga Rosella (Hibiscus sabdariffa L.) Novia Hilma; Nuri Nuri; Endah Puspitasari; Indah Yulia Ningsih
Pustaka Kesehatan Vol 6 No 2 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i2.7574

Abstract

Jati belanda leaves (Guazuma ulmifolia Lmk.) and Roselle petals (Hibiscus sabdariffa L.) have been known for their activity as antihyperlipidemia with different mechanisms. Combination of these plants was expected to has a synergy effect in reducing cholesterol. However, the toxicity of jati belanda leaves and roselle petals extract combination must be investigated for knowing its safety. Based on the acute toxicity test, the LD50 value of jati belanda leaves and roselle petals extract combination was >5,000 mg/kg bw. It was classified as category 5 or unclassified. This research aimed to study about its effect in cardiac by cardiac histopathology examinations of the rats. The result showed that there was no cardiac histopathology changes of the rats at doses 5,000 mg/kg bw. Keywords: jati belanda leaves, roselle petals, acute toxicity, cardiac histopathology
Pengaruh Ekstrak Metanol Biji Pepaya Tua dan Ekstrak Metanol Biji Pepaya Muda (Carica papaya L.) terhadap Kualitas dan Kuantitas Spermatozoa Tikus Putih Jantan (Rattus norvegicus) (Effect of Methanolic Extract of Ripe and Raw Papaya Seeds (Carica papaya Dwi Novita Wijayanti; Siti Muslichah; Endah Puspitasari
Pustaka Kesehatan Vol 4 No 3 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

This research was aimed to determine the effect of antifertility among the methanol extract of ripe and raw papaya seeds in decreasing the quality and quantity of spermatozoa. Animal test rats were divided into 3 groups: negative control (CMC-Na 1%) and the treatment groups given methanol extract of ripe and raw papaya seeds extract. The extracts were given orally for 20 days. On the 21st day, mice were dissected to obtain sperm from the cauda epididymis, then were observed for the quality (motility, viability, morphology) and the quantity of spermatozoa. One-Way ANOVA followed by Post Hoc Test LSD analysis showed that was better for its activity then that of the ripe papaya seed extract. Keywords: ripe papaya seed, raw papaya seed, quality, quantity, spermatozoa
Penetapan Kadar Fenol Total dan Pengujian Aktivitas Antioksidan Minyak Jahe Gajah (Zingiber officinale var. officinale) (Determination of Total Phenolic Content and Antioxidant Activity of Jahe Gajah (Zingiber officinale var. officinale) Oil) Tsabit Barki; Nia Kristiningrum; Endah Puspitasari; Fifteen Aprila Fajrin
Pustaka Kesehatan Vol 5 No 3 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i3.5897

Abstract

Jahe gajah (Zingiber officinale var. officinale) is one of export commodity plant in Indonesia. Part of jahe gajah often to be used is the rhizome, that content lots of essentials oil. One of the essential oil content is a phenolic compound. Phenolic compound is a natural product function as antioxidant reducing oxidative damage in the body that cause inflammation. The aim of this study was to determine the total phenolic content and antioxidant activity of jahe gajah oil. The total phenol content was determined using Folin-Ciocalteu method and the antioxidant activity assay was done using DPPH method. The total phenol content for jahe gajah oil was 0.626 ± 0.022 mg GAE/g . The IC50 of jahe gajah oil was 5,766 ± 0,087 µl/ml. Antioxidant activity from jahe gajah oil was suggested to be contributed by the phenolic compound and result from synergistic action of all constituents. Keywords: essential oil, aktivitas antioxidant, phenolic content
Aktivitas Antioksidan dan Kemampuan Proteksi terhadap Kerusakan DNA dari Protein Isolat Biji Melinjo (Gnetum Gnemon L.) Terhidrolisis Menggunakan Alkalase Terimobilisasi (Antioxidant Activity and Protection Ability Against DNA Damage of Isolate Protein Me Anandini Aulia Safiera; Endah Puspitasari; Tri Agus Siswoyo
Pustaka Kesehatan Vol 5 No 2 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i2.5870

Abstract

Hydrolyzed protein isolates of melinjo seed using free alcalase has been known to have antioxidant potential. However, free alcalase can only be used once. Enzymes immobilization technique with entrapment method would overcome this problem. The immobilized enzyme characteristic was observed by SEM and hydrolysis degree value. The success of hydrolysis process was observed by SDS-PAGE. Antioxidant activity test was determined by the ability to inhibit hydroxyl radical and protection against DNA damage in 1% agarose gel. The result showed that protein isolates from melinjo seed had been successfully hydrolyzed by immobilized alcalase. Following hydrolysis showed that immobilized alcalase was effective to hydrolyze protein up to four times. Based on hydroxyl radical reduction activity test, antioxidant activity of hydrolyzed protein using immobilized alcalase had the same as that of glutathione as positive control antioxidant activity. In addition, hydrolyzed protein using immobilized alcalase had the ability toprotect DNA damage which showed by reversible change of supercoil band. Keywords: melinjo seed, hydrolyzed protein, immobilized alcalase, antioxidant
Aktivitas Inhibitor α-Glukosidase Ekstrak Etanol Daun Kenitu (Chrysophyllum cainito L.) (α-Glucosidase Inhibitor Activity of Ethanol Extract Kenitu Leaves (Chrysophyllum cainito L.)) Fatimatuz Zuhro; Endah Puspitasari; Siti Muslichah; Moch. Amrun Hidayat
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Kenitu (Chrysophyllum cainito L.) grows in Jember region, East Java, with several variants which can be distinguished from the color and shape of the fruit. They are small round (BK), large round (BB), green oval (HL), and red round (MB). Kenitu leaves have been shown to provide antidiabetic activity in vivo, but the molecular mechanism still unknown yet. One of the oral antidiabetic is a class of α-glucosidase inhibitor. This research aimed to test the activity of Saccharomyces cerevisiae α-glucosidase inhibitor of several variants of kenitu leaves in Jember, as well as phytochemical screening. Simplicia powder was extracted using 70% ethanol with ultrasonication method to obtain a thick extract. α-glucosidase inhibitor activity test was carried out on the negative control solution (without extract) and the sample solution (extract) using chromogenic method. Phytochemical screening was done by TLC and tube test. IC50 value of ethanol extract kenitu leaves variant BK, BB, HL, and MB were 5.476 ± 0.039, 4.869 ± 0.018, 9.465 ± 0.012, and 11.836 ± 0.048 µg/ml, respectively. Based on the LSD test, IC50 values between variants of kenitu leaves were significantly different (p < 0,05). Phytochemical screening result showed that ethanol extract kenitu leaves contain saponin, triterpenoids, flavonoids, and phenolic compounds. Those compounds were suspected to contribute on α-glucosidase inhibitor activity, but more study still needed to isolate the active compounds and evaluate each isolate for its α-glucosidase inhibitor activity. Keywords: α-glucosidase inhibitor, kenitu leaves, ethanol extract
Hidrolisis Protein Isolat Biji Melinjo (Gnetum gnemon L.) menggunakan Alkalase Terimobilisasi dan Aktivitasnya sebagai Antihipertensi (Hydrolysis of Melinjo Seed (Gnetum gnemon L.) Isolated-Protein using Immobilized Alcalase and Its Activity as Antihyper Nur Fauziah Matra; Endah Puspitasari; Tri Agus Siswoyo
Pustaka Kesehatan Vol 6 No 1 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i1.6612

Abstract

Hypertension is ranked third on the cause of death in Indonesia for all ages (6.8 %). Protein isolate of melinjo seeds (Gnetum gnemon L.) hydrolyzed with free alcalase has been known to have antihypertensive activity. However, the use of free alcalase is uneconomical since it can only be used once, thus we studied the immobilized alcalase effectivity to hydrolise the melinjo seeds protein isolate to be used as angiotensin converting enzyme (ACE) inhibitor. The immobilization method used entrapment method with DMDMOS/TMOS as matrix. Characterization of the immobilized enzyme was observed by FTIR and effectiveness of repeated hydrolysis was observed by the value of the degree of hydrolysis. The success of the hydrolysis process was determined by SDS-PAGE electrophoresis. Antihypertensive activity test was determined by the ability to inhibit ACE. The results showed that the alcalase has been immobilized in the matrix of silane and SDS-PAGE protein profile showed melinjo seed proteins had been successfully hydrolyzed. Hydrolysis process repeatedly demonstrated that immobilized alcalase was effective to hydrolyze protein isolates of melinjo seeds twice. Based on ACE inhibitory test, there were no significant differences between the protein isolate hydrolyzed by immobilized alcalase (Gg-PH), protein isolates hydrolyzed with free alcalase (Gg-PHB), and captopril. These findings suggest that the antihypertensive activity in Gg-PH is the same with that of captopril. Keywords: hypertension, antihypertensive, melinjo, enzymes immobilization, ACE inhibitor
Co-Authors . Anindyajati Aditya Fitriasari Aditya Fitriasari Agustina, Bayu Agustina, Bayu Anandini Aulia Safiera Anindyajati Anindyajati Ari Satia Nugraha Azis, Deri Abdul Banun Kusumawardani Baroroh, Rizqy Kiromin Bawon Triatmoko Bawon Triatmoko Bawon Triatmoko Binar Asrining Dhiani Dani Usmar Dani Usmar, Dani Dea Ajeng Pravita Suendi Dewi Dianasari Dewi Dianasari Dewi Dianasari Diana Holidah Dianasari, Dewi Dwi Merry Christmarini Robin Dwi Novita Wijayanti Dyaningtyas D. P. Putri Dyaningtyas Dewi P. Putri Dyaningtyas Dewi Putri Pamungkas Edy Meiyanto Edy Meiyanto Endah Dwi Setyowati Endah Dwi Setyowati ESTIKA YUNINDARWATI Evi Umayah Ulfa Fadhila, Nadila Ikhda Ramadhani Faris Rudiana, Irfan Fatimatuz Zuhro Fauzia Ken Nasititi Febi, Qonita Nafilah Fifteen Aprila Fajrin Hasanah, Fitrianti Milhatul Hidayatul Ulyah Indah Yulia Ningsih Irma Josefina Savitri Karmilasari, Alviani Dwi Labes, Antje Lestyo Wulandari Lukmanto, Lukmanto Maharany, Dinda Mochammad Amrun Hidayat Muhammad Zaki Rahman Nahraeni, Wini Nasititi, Fauzia Ken Nia Kristiningrum Novan Eko Setiyanggono Novia Hilma Nur Fauziah Matra Nur Ismiyati Nur Ismiyati Nuri . Nuri . Nuri Nuri Nuri Nuri Nuri Nuri Nuri Nuri Oktarianti, Rike Oktavia, Melinda Dwi Putri Putri Aditama, Tiara Sagita Ramadhan, Andika Dewi Rifki Febriansah Rifki Febriansah Rosidah, Malihatul Rudiana, Irfan Faris Sarmoko Sarmoko Senjarini, Kartika Siti Masithoh Siti Muslichah Siti Nurjannah Siti Sarah, Siti Sugeng Riyanto Sugeng Riyanto Tanfidz Alishlah, Tanfidz Tazkiyatul Fitriyah Tri Agus Siswoyo Tsabit Barki Viddy Agustian Rosyidi Wathon, Syubbanul Yuni Retnaningtyas Zahra, Nurul Fatimatuz