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Sjarief Widjaja
Chemical Engineering Department, Institut Teknologi Sepuluh Nopember, Kampus ITS Sukolilo, Surabaya 6011
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Journal : Makara Journal of Science

 1 
PENINGKATAN UNJUK KERJA HIDROLISIS ENZIMATIK JERAMI PADI MENGGUNAKAN CAMPURAN SELULASE KASAR DARI Trichoderma reesei DAN Aspergillus niger Anwar, Nadiem; Widjaja, Arief; Winardi, Sugeng
Makara Journal of Science Vol. 14, No. 2
Publisher : UI Scholars Hub

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Abstract

Increasing the Performance of Enzymatic Hydrolysis of Rice Straw Using Mixed Crude Cellulases from Trichoderma reesei and Aspergillus niger. The objective of this work is to compare the effectiveness of mixed crude enzyme cellulase from T. reesei and A. niger with commercial enzyme from A. niger, and to investigate effect of enzyme to substrate ratio to performance of enzymatic hydrolysis of rice straw. The commercial enzyme from Fluka Biochemica was used, and crude enzyme were prepared by solid fermentation with simple media. Before hydrolized, the rice straw was grinded and sieved and then heated at 85 o C with 2% sodium hydroxide for six hours. Hydrolysis was conducted in 300 mL beaker flask equipped with mechanical stirrer. Samples were analyzed by dinitrosalicylic acid method and measured by spectrophotometer. Both of commercial and mixed crude enzyme show that, the higher enzyme to substrate ratio was higher the glucose concentration obtained. However, ratio of glucose obtained to enzyme used become smaller. The mixture of crude enzyme from T. reesesi dan A. niger that produced in this work was two fold more effective to hydrolyze rice straw than using cellulase enzyme of A. niger from Fluka Biochemika
Co-Authors Achmad Chumaidi, Achmad Achmad Zubaydi Adi Soeprijanto Aditya Dwi Saputra Adrian Nur Adrian Nur Afan Hamzah, Afan Agra Bachtiar Ahmad Zubaydi Akbarningrum Fatmawati Akbarningrum Fatmawati Akhmad Basuki Widodo Al-Ghifari, Admiral Yagi Anwar, Nadiem Aparamarta, Hakun Wirawasista Aparamarta, Hakun Wirawista Apol, Tabina Raissa Ari Anggoro Arif , Irfan Syarief Artaraharja, Yoseph Daniswara Bagiyo Suwasono Bagiyo Suwasono Bagiyo Suwasono Bagiyo Suwasono, Bagiyo Baiq Firyal Salsabilla Safitri Candra Wijaya, Candra Debra Arlin Puspasari Debra Arlin Puspasari Denistira Fazlur Rahman Desi Liah Martasari Desy Nurwijayanti Dwina Moentamaria Eny Setyowati F Ferry Fadilah, Siska Nuri Firmansyah, Arfian Alwi Fredi Susanto Hanny F. Sangian Hasanudin Hellen Kartika Dewi Hellen Kartika Dewi Hermawan, Yuda Apri Heru Setyawan I Nyoman Budiantara I. Wuled Lenggoro Ilham Alamsah Ilham Alamsyah Integrity, Justian Immanuel Irfan Albana Irfin, Zakijah Irma Nurhanifah Fenda Putri Jayyid Zuhdan Junaidi Kristian Junaidy Kristian Junaidy Kristian Junianti, Fitri Kamila Adila Muslim Karima, Nur Kurniawan Candra Eka Prasetya Mamuji Putra Laila Nur Rahmah Laila Nur Rahmah Lidya Lorenta Sitompul Lieke Riadi M Musmariadi M. Rasyid, Daniel M. Zaed Yuliadi Ma'ruf, Buana Marcus Tukan Maulidarsyah, Muhammad Fauzan Muhammad Nurul Hakim Muharja, Maktum Mukti Mulyawan Nadiem Anwar Novandira, Belva Angeline Nur Fadhilah Nur Fadhilah Nurkhamidah , Siti Nurul Maziyah Nurul Maziyah Panunggal, Eko Patdono Suwignjo Pertiwi, Urania Noor Lintang Rachma, Fanina Aulia Ratna Purwaningsih Ratna Purwaningsih Ratna Purwaningsih Rizki Fitria Darmayanti Rohmah, Ika Putri Nikmatur Samsudin Affandi Sangadji, Ningsi Lick Setiyo Gunawan Silvya Yusnica Agnesty Silvya Yusnica Agnesty Silvya Yusnica Agnesty Silvya Yusnica Agnesty Siska Nuri Fadilah Siska Nuri Fadilah Soedarmodjo, Thea Prastiwi Soegiono, Soegiono Sri Gunani Partiwi Sri Gunani Partiwi Sugeng Winardi Sukma Rahma Sukma Rahma Sukmawati Rahma Tantular Nurtono Thea Prastiwi Soedarmodjo Tirta Sumadilaga, Ibrahim Tri Achmadi Tri Widjaja Winardi, Sugeng Yakub Hendrikson Manurung Yakub Hendrikson Manurung Yuliadi, M. Zaed Zaed Yuliadi Zaed Yuliadi, Zaed Zubaydi, Ahmad Zuhroh, Saiyyidah Tus