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Seleksi Komposisi Medium Pertumbuhan dan Bahan Pembawa untuk Formulasi Cendawan Agens Hayati Fusarium oxysporum Non-Patogenik P21a Fany Juliarti Panjaitan; Suryo Wiyono; Rahayu Widyastuti
Jurnal Fitopatologi Indonesia Vol 15 No 2 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.16 KB) | DOI: 10.14692/jfi.15.2.44-52

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Selection of Compositions of Growth Medium and Carriers for Formulation of Biological Agents of Non-Pathogenic Fusarium oxysporum P21aThe potency of non-pathogenic Fusarium oxysporum P21a (NPFo P21a) as a biological control agent has prospect to be developed commercially. The growth medium and carrier are the critical factor to formulate biological control. This study aimed to obtain the best solid medium dan lighting type for mycelium growth and sporulation as well as to determine the carrier and storage temperature which is suitable for NPFo P21a toward the survival of propagule and germination of shallot. The results showed that M2 medium treatment (rice grain:rice bran; 20:1 w/w) was the best medium for mycelium growth. The M3 medium treatment (20:2 w/w) was the best medium for sporulation of NPFo P21a. The near-UV lighting treatment was able to stimulate the macroconidium production of NPFo P21a. Talc-based formulation and the storage at 20 °C showed the best shelf-life for NPFo P21a with density of viable propagule and shallot germination better.
KEANEKARAGAMAN ENZIM INVERTASE, PENGEMBANGAN STRAIN UNGGUL DAN TEKNOLOGI PRODUKSINYA Wilhelmus Terang Arga Sanjaya; . Giyanto; Rahayu Widyastuti; Dwi Andreas Santosa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1328.71 KB) | DOI: 10.29122/jbbi.v7i1.3705

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Invertase Diversity, Novel Strain and Production Technology DevelopmentInvertase or β-fructofuranosidase (E.C.3.2.1.2.6) have been a valuable enzyme in food industry, so that research about enhancing invertase activity on an industrial scale has been reported massively. So far, the production of the invertase enzyme is highly dependent on the potential activity of the invertase enzyme derived from microorganisms. The development of enzyme production technology is also a concern in invertase research to obtain an efficient and inexpensive production system. So far, various developments in enzyme production technology and enzyme utilization have been carried out, including various innovations in immobilizing and increasing the stabilization of the invertase enzyme during the production process. The development of superior strains and enzyme production technology continues amid the discovery of the latest approaches such as genetic engineering, protein modification, and nanotechnology. This paper aims to discuss invertase variation from various organisms, its correlation to novel microbial strain development to increase invertase production and invertase enzyme production technologies development including immobilization technology and stabilization of invertase enzymes.Keywords: β-fructofuranosidase, ezyme production, fermentation, immobilitation, invertase geneABSTRAKInvertase atau β-fructofuranosidase (E.C.3.2.1.2.6) merupakan enzim yang sangat penting dalam dunia industri pangan sehingga berbagai optimasi aktivitas enzim untuk produksi dalam skala industri terus dilakukan. Produksi enzim invertase hingga sejauh ini sangat bergantung oleh potensi aktivitas enzim invertase yang diambil dari mikroorganisme. Selain itu, pengembangan teknologi produksi juga menjadi perhatian dalam pengembangan produksi dan pemanfaatan enzim untuk mendapatkan sistem produksi yang efisien dan murah. Sejauh ini berbagai pengembangan teknologi produksi enzim dan pemanfaatan enzim telah dilakukan, diantaranya dengan berbagai inovasi dalam melakukan imobilisasi dan peningkatan stabilisasi enzim invertase selama proses produksi. Pengembangan strain unggul dan teknologi produksi enzim terus dilakukan di tengah ditemukannya pendekatan-pendekatan terkini seperti rekayasa genentika, modifikasi protein, dan teknologi nano. Artikel ini bertujuan untuk membahas variasi enzim invertase dari berbagai organisme, korelasi pengembangan strain mikrobia unggul terhadap peningkatan produksi enzim invertase, dan perkembangan teknologi produksi enzim invertase meliputi teknologi imobilisasi dan stabilisasi enzim invertase.
Abundance, Biomass and Diversity of Soil Fauna at Different Ecosystems in Jakenan, Pati, Central Java Rahayu Widyastuti
Jurnal Ilmu Tanah dan Lingkungan Vol 6 No 1 (2004): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1010.414 KB) | DOI: 10.29244/jitl.6.1.1-6

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The assessment of soil fauna in three different ecosystems namely teak forest, home garden and fallow paddy field had been studied in Pati, Central Java. The soil fauna was collected using a soil corer of 20 cm diameter to the depth of 0-15 cm from 5 randomized points in the above ecosystems. The soil fauna was t$en extracted in a Berlese funnel extractor. Soil fauna in the home garden showed the highest abundance (2 940 individual m--), followed by teak forest (2 340 individual m-?) and fallow paddy (1 790 individual m-?). The home garden had also a higher soil fauna diversity (2.06) compared to the teak forest (1.82) and fallow paddy (1.67). In terms of soil fauna biomass, the teak forest had a higher value (961 mg m-3 compared to the home garden (368 mg m-3 and fallow paddy (309 mg m"). In these three ecosystems, two fauna groups, i.e. Collembola and Acari were the most abundant animals. Poor vegetation cover tends to reduce the population of soil fauna in the fallow paddy. field, whereas vegetation, found in the home garden and teak forest protected the soil surface from direct sunshine and maintained soil moisture. This condition presumably provided a more favorable habitat for soil fauna. Thus, vegetation cover appears important to maintain soil moisture and soil living-organisms. Keywords: soil fauna, abundance, diversity, ecosystem, vegetation cover
Population Dynamics of Microarthropods (Oribatida and Collembola) in Rainfed Paddy Field Ecosystem in Pati, Central Java Rahayu Widyastuti
Jurnal Ilmu Tanah dan Lingkungan Vol 7 No 1 (2005): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.761 KB) | DOI: 10.29244/jitl.7.1.11-14

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A study on population dynamics of microarthropods (oribatids and collembolans) in rainfed paddy field ecosystem inPati, Central Java was undertaken with the aim to evaluate which group of soil microarthropods that dominated in each phase of rain fed paddy field. The soil fauna was collected using a soil corer of20 cm diameter to a depth of 0-15 cm, every 30 days.In the fallow, soil fauna was sampled from 4 randomized points per plot, meanwhile in the rice field phases (dry seeded rice and transplanted rice), it was sampled from 4 randomized points in the fields and in the bunds, respectively. per plot. Soil microarthropods in each season were then extracted in a Berlese funnel extractor.During the fallow phase, the dominant soil microarthropods were Oribatida of the Acari (1200 individual m,l) andsome groups of Collembola, namely Hypogastruridae (/200 individual m:2), Entomobryidae (500 individual m,l) andIsotomidae (620 individual m,2), meanwhile Sminthuridae of Collembola did not appear in this phase. In the rice phase,however, Sminthuridae of the Collembola occurred in high numbers, i.e., 860 individual m,2 (field) and 6/0 individual m,l(bund).Regarding the population dynamics of soil microarthropods in rainfed paddy field, we can assumed that oribatids and some groups of collembolans (Hypogastruridae, Entomobryidae and Isotomidae) play an important role in improving the nutrient availability during the fallow phase. meanwhile the Sminthuridae of collembolan seemed to play such role in the rice field phase. more than the other soil microarthropods.
DINAMIKA KELIMPAHAN ORIBATIDA PADA AREA PERKEBUNAN KELAPA SAWIT DI KECAMATAN BAJUBANG BATANGHARI JAMBI Yuni Lisafitri; Rahayu Widyastuti; Dwi Andreas Santosa
Jurnal Ilmu Tanah dan Lingkungan Vol 17 No 1 (2015): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.432 KB) | DOI: 10.29244/jitl.17.1.33-38

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Oribatids are one of the important fauna groups in the soil. They have an important role in the decomposition of organic matter in the soil. The objection of this research was to study population dynamics of Oribatids through determining the abundance and diversity of Oribatids and to know the effect of environmental factors on Oribatids in oil palm plantation at Bajubang, Batanghari, Jambi. Soil and litter samples were taken from four different locations (25 m x 25 m) and from each location three samples were taken from litter lanes and open lanes, respectively (the size was 16 x 16 cm with a depth of soil is 5 cm). The samples were extracted using Kempson extractor. Identification of Oribatids was done to the family level and the diversity of Oribatids was measured using Shannon’s diversity index. The population dynamic of Oribatids showed a fluctuation in the sampling period. When rainfall increased >200 mm did not follow by increasing abundance and diversity (number of the family) of Oribatid mites. When rainfall between 100 – 200 mm was the optimal condition for Oribatids so abundance and diversity (the number of the family) of Oribatids increased. But, when rainfall <100 mm especially in February 2014 (the lowest rainfall 1 mm), abundance and diversity (number of the family) of Oribatids was lowest (330 individual m-2; 7 families) compared to another month. It was assumed that Oribatids may migrate into the deep soil to avoid a harsh environmental condition. Abundance and diversity (number of the family) of oribatids were significantly higher in litter lanes (4,560 individual m-2; 25 families) than in open lanes (1,570 individual m-2; 14 families). The availability of litter in the litter lanes was higher than in open lanes. In this research, There are 3 families that most abundance and always found on the sampling period, they are Scheloribatidae, Mycobatidae and Galumniidae. Keywords: Abundance, diversity, oil palm plantation, Oribatids, soil fauna
KEMAMPUAN BAKTERI DIAZOTROF ENDOFIT UNTUK MENINGKATKAN PERTUMBUHAN VEGETATIF BIBIT KELAPA SAWIT (Elaeis guineensis jacq) Andreas Panjaitan; Iswandi Anas; Rahayu Widyastuti; Wiwik Eko Widayati
Jurnal Ilmu Tanah dan Lingkungan Vol 17 No 1 (2015): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.275 KB) | DOI: 10.29244/jitl.17.1.1-7

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Endophytic diazotrophic bacteria are free-living atmospheric dinitrogen fixing bacteria that lived and colonized between living plant cells and do not contribute pathogenic effects to its host. The ability of endophytic diazotrophic bacteria in providing nitrogen as a nutrient for crop plays an important role in reducing the dependency of inorganic nitrogen fertilizer. The aim of this experiment was to study the ability of endophytic diazotrophic bacteria isolate to stimulate vegetative growth of oil palm seedlings at four levels inorganic nitrogen fertilizer application. Experiment was conducted at the greenhouse IPB Campus, Cikabayan, Darmaga with the altitude of 250 m above sea level and at the Soil Fertility Laboratory, the Departement of Soil Science and Land Resources, Faculty of Agriculture, Bogor Agricultural University (IPB) and at the Laboratory of Biotechnology, Indonesian Sugarcane Research Centre (P3GI) Pasuruan, East Java. This experiment using Completely Randomized Block Design (CRBD) consisted of two factors with three replications. The first factor was inorganic nitrogen levels consisted of four levels (0, 50%, 75% and 100% of the recommended dose). The second factor was inoculation with endophytic diazotrophic bacterial isolates consisted of four levels; without inoculation, inoculated with isolate 1, isolate 2 and isolate 3. The results of the experiment showed that the inoculation with bacterial isolate 2 without N fertilizer application gave the best results to increase the diameter of the palm weevil, palm height, and dry weight. In addition to that, the application of endophytic diazotrophic bacterial isolate was able to promote vegetative growth of palm oil seedling. Keywords: Endophytic diazotrophic bacteria, nutrient up-take, oil palm seedling, vegetative growth
KELIMPAHAN DAN KEANEKARAGAMAN MIKROARTHROPODA PADA MIKROHABITAT KELAPA SAWIT Titik Tri Wahyuni; Rahayu Widyastuti; Dwi Andreas Santosa
Jurnal Ilmu Tanah dan Lingkungan Vol 17 No 2 (2015): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.98 KB) | DOI: 10.29244/jitl.17.2.54-59

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Acari dan Collembola merupakan mikroarthropoda yang hidup di tanah dan lapisan serasah. Dalam ekosistem mikroarthropoda berperan aktif dalam proses dekomposisi yaitu dalam fragmentasi bahan organik sehingga dapat mempercepat penghancuran bahan organik. Penelitian ini bertujuan untuk mempelajari kelimpahan dan keanekaragaman mikroarthropoda pada berbagai mikrohabitat kelapa sawit. Penelitian ini terdiri dari 3 tahap yaitu pengambilan sampel, ekstraksi dan identifikasi fauna tanah. Plot pengambilan sampel diambil dengan jarak 50 x 50 m yang terdiri dari 5 titik, setiap titik terdiri dari 11 mikrohabitat. Sampel diambil pada beberapa mikrohabitat kelapa sawit yaitu tanah dengan jarak 0 m; 1 m; 3m dari tanaman kelapa sawit, gawangan kompos, pelepah pada ketinggian 30 cm; 90 cm; 150 cm, epifit pada ketinggian 30 cm; 90 cm; 150 cm dan tonggak. Sampel diambil pada bulan Januari 2014. Ekstraksi mikroarthropoda menggunakan Kempson Extractor, sedangkan identifikasi dilakukan sampai tingkat subordo untuk Acari dan famili untuk Collembola. Kelimpahan dan keanekaragaman mikroarthropoda dihitung menggunakan rumus kelimpahan menurut Meyer dan indeks keanekaragaman Shanonn (Shannon’s diversity index). Untuk mengetahui perbedaan kelimpahan dan keanekaragaman mikroarthropoda di berbagai mikrohabitat digunakan analisis sidik ragam satu arah (one-way ANOVA) dan uji jarak berganda Duncan. Hasil penelitian menunjukkan bahwa kelimpahan mikroarthropoda tertinggi ditemukan pada pelepah dan epifit. Kelimpahan Acari tertinggi ditemukan pada mikrohabitat epifit dengan ketinggian 30 cm yaitu 4,408 individu m-2, sedangkan kelimpahan Collembola tertinggi ditemukan pada mikrohabitat pelepah dengan ketinggian 150 cm yaitu 6,173 individu m-2. Kondisi mikroklimat pada mikrohabitat pelepah dan epifit lebih sesuai untuk kehidupan mikroarthropoda dibandingkan dengan mikrohabitat yang diteliti. Keanekaragaman mikroarthropoda pada mikrohabitat kelapa sawit tergolong rendah berdasarkan indeks keanekaragaman Shannon. Kata kunci: Kelimpahan, keanekaragaman, mikroarthropoda, mikrohabitat, kelapa sawit
Substitusi Pupuk Kimia pada Budidaya Padi SRI Pengaruhnya Terhadap Pertumbuhan, Produksi dan Organisme Tanah: Substitution of Inorganic Fertilizer In SRI Paddy Cultivation Effect on Growth, Production and Soil Organisms Rury Kurniawan; Iswandi Anas; Rahayu Widyastuti; Atang Sutandi
Jurnal Ilmu Tanah dan Lingkungan Vol 19 No 1 (2017): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.392 KB) | DOI: 10.29244/jitl.19.1.46-50

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Penelitian ini bertujuan mempelajari pengaruh budidaya SRI dibandingkan metode konvensional dengan berbagai taraf pemupukan terhadap pertumbuhan dan produksi padi serta mempelajari aktivitas dan populasi organisme tanah. System of Rice Intensification (SRI) merupakan inovasi dalam system budidaya padi yang masih berkembang terus namun telah mampu meningkatkan produksi. Penelitian ini dilaksanakan sejak Desember 2014 sampai dengan April 2015 pada lahan sawah irigasi kecamatan Pakem Kabupaten Sleman, Provinsi D.I Yogyakarta. Penelitian ini menggunakan rancangan split plot. Petak utama adalah metode budidaya padi SRI dan konvensional, dan sebagai anak petak adalah empat taraf pemupukan, yaitu pupuk anorganik dosis rekomendasi dan dikombinasi dengan pupuk organik serta pupuk organik hayati. Hasil penelitian menunjukkan tinggi tanaman, jumlah anakan, jumlah anakan produktif dan hasil panen pada budidaya SRI signifikan lebih tinggi dibandingkan budidaya konvensional. Metode budidaya SRI mampu meningkatkan hasil gabah lebih tinggi. Aktivitas dan populasi organisme tanah juga tidak menunjukan perbedaan yang nyata pada penerapan metode budidaya dan aplikasi pemupukan yang dilakukan. Kata kunci: Pertumbuhan, produksi dan organisme tanah, System of Rice Intensification (SRI)
Uji Fitopatogenitas, Hemolisis serta Kemampuan Mikrob dalam Melarutkan Fosfat dan Kalium: Test of Phytopathogenicity, Hemolysis and Microbial Ability in Solubilizing Phosphate and Potassium Desak Ketut Tristiana Sukmadewi; Iswandi Anas; Rahayu Widyastuti; Ania Cintaresmini
Jurnal Ilmu Tanah dan Lingkungan Vol 19 No 2 (2017): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (535.167 KB) | DOI: 10.29244/jitl.19.2.68-73

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Soil microbes have an important role in the cycle of phosphorus and potassium. Therefore, a multifunctional microbe is required to have two or more functions. Before a multifunctional test is performed, the microbes must be tested and confirmed, they are not pathogenic. The purpose of this research was to study phytopathogenicity, hemolysis and microbial ability in solubilizing phosphate and phosphate. The research procedure consisted of phytopathogenicity test, hemolysis test, the test of phosphate solubilizing ability on Pikovskaya solid medium and potassium solubilizing ability on Alexandrov solid medium. Based on the results of phytopatgonecity tests on bacteria and fungi, all isolates are non-phytopathogenic. From the hemolysis test of bacterial isolate BPK 2, BPK 6 and BPK 7 caused total hemolysis. Based on the hemolysis test of fungi isolate SSIO 6 caused total hemolysis, FPF E1 and JK 6 caused partial hemolysis. Isolate BPK 5 has the highest index in solubilizing potassium (1.375), while isolate BPF 9 has the highest index in solubilizing phosphate (1.533). Keywords: Microbe, multifunctional, pathogenicity, phosphate solubilizing, potassium solubilizing
Populasi Mikrob Fungsional pada Rhizosfer Kelapa Sawit di Lahan Gambut Riau: Functional Microbial Population on Oil Palm Rhizosphere in Riau Peatlands Morgan Ohiwal; Rahayu Widyastuti; Supiandi Sabiham
Jurnal Ilmu Tanah dan Lingkungan Vol 19 No 2 (2017): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (657.056 KB) | DOI: 10.29244/jitl.19.2.74-80

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Oil palm growth in peatland may influence the presence of microbial populations. Root exudate is one of the organic compounds that is released by oil palm roots in the rhizosphere area where it can be used as a nutrient for microbes to survive in peatland. The study was conducted to study functional microbial population in the rhizosphere area of oil palm plantation aged <6, 9−15, and >15 years in Riau peatlands. The highest microbial populations were found in oil palm plantation aged <6 years at peat thicknesses of <3 and >3 m, which was respectively 10.3 x 106 and 5.7 x 106 CFU g-1. The highest cellulolytic microbial population was found in oil palm plantation aged <6 years at peat thicknesses of <3 and >3m, which was respectively 17.4 x 104 and 11.4 x 104 CFU g-1. The highest Azotobacter population was found in oil palm plantation aged >15 years at peat thicknesses of <3 and >3m, which was respectively 9.4 x 105 and 12.5 x 105 CFU g-1. The highest phosphate solubilizing microbial population was found in oil palm plantation aged <6 years at peat thicknesses of <3 and >3m, which was respectively 11.4 x 104 and 13.2 x 104 CFU g-1. The highest fungal population was found in oil palm plantation aged <6 years at peat thicknesses of <3 m and >3 m, which was respectively 11.4 x 104 CFU g-1 and 17.3 x 104 CFU g-1. The highest white-rot fungal population was found in oil palm plantation aged 9−15 years at peat thicknesses of <3 m and >3 m, which was respectively 8.7 x 103 CFU g-1 and 9.4 x 103 CFU g-1. In conclusion, the highest microbial population was dominantly found in oil palm plantation aged <6 years. Keywords: Microbial populations, oil palm plantation, peat thickness, rhizosphere area
Co-Authors . Anandyawati . Giyanto . Giyanto A M Zuhud, Ervizal Abied Khafidhan Adiyaksa, Ivan Khofian AHMAD JUNAEDI Ana Khalisha Anandyawati, . Anas Iswandi Andreas Panjaitan Andreas Panjaitan Andreas Panjaitan, Andreas Ania Cintaresmini Ania Citraresmini Ania Citraresmini Antonius Suwanto Apsari Putri Wulandari Aris Tri Wahyudi Atang Sutandi Atit Kanti Aviantara, Dwindrata B. Azra Zahrah Nadhirah Ikhwani Bria, Deseriana Budi Nugroho Budi Nugroho Dadang . DADANG DADANG Darmawan Darwis Delima Napitupulu Delima Napitupulu Devanda Ayu Lidya Permata Putri Dewi Sekar Pengerteni Dewi, Tirta Kumala Dhanti Hanifa Muslimah Dimas Syahiddin DWI ANDREAS SANTOSA Dwi Andreas Santosa Dwi Wulandari Edy Hartulistiyoso Eka Lupitasari Endang Sulistyorini Endang Sulistyorini Enny Widyati Enok Sumarsih ERNIN HIDAYATI Erwinda Erwinda Evi Ambarsari Fahrizal Hazra Fajri, Ihsanul Fany Juliarti Panjaitan Giyanto Gunawan Djajakirana Hariyadi Heru Bagus Pulunggono I Made Sudiana I Nyoman Sujana Ida Widiyawati Idris Idris Idris, Komaruddin Indri Hapsari Fitriyani Irawati, Arfi Irdika Mansur Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Chaniago Jekvy Hendra Jekvy Hendra Joko Warino Kartika, Sari Yulia Khairiyah, Yaumil Laksmita Prima Santi MAIPA DIA PATI Masaru Kobayashi Misbakhul Bait Nabila Dianisa Niken Puspita sari Nofrifaldi Ohiwal, Morgan Priyo Cahyono Putri Wulandari, Dwi Ramadhani Sri Astuti, Andi Febrianti Ratnawati Lantifasari Remila Selvany Risa Rosita Rohani Cinta Badia Ginting Rumella Simarmata Rury Kurniawan Safira Eka Aprianti Sanjaya, Wilhelmus Terang Arga Sarah Sakinah Umadi Sari Yulia Kartika Sarjiya Antonius Satriyas Ilyas Satya Nugroho Soetanto Abdoellah Sri Sulisawati Suciati, Fuzi Sugeng Santoso Sugiarto, Petrus Sugiyanta Sukmadewi, Desak Ketut Tristiana Sumarsih, Enok Suryo Wiyono Syaiful Anwar Taufiq Hidayat Tita Puspitasari Titik Tri Wahyuni Umadi, Sarah Sakinah Umi Hidayati Umi Hidayati Utami, Aditya Dyah Widrializa, . Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Winda Ika Susanti Wiwik Eko Widayati Yayuk R. Suhardjono Yayuk Rahayuningsih Suhardjono Yayuk Rahayuningsih Suhardjono Yuni Lisafitri Yusuf, Sri Malahayati Zuhud, Evrizal A M