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All Journal Biota Biota ULIN: Jurnal Hutan Tropis BERNAS: Jurnal Pengabdian Kepada Masyarakat Jurnal Sylva Scienteae Jurnal Pengabdian Ilung (Inovasi Lahan Basah Unggul) JURNAL HUTAN TROPIS Repong Damar: Jurnal Pengabdian Kehutanan dan Lingkungan
Adistina Fitriani
Universitas Lambung Mangkurat
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Induction of in Vitro Germination of Tandui (Mangifera rufocostata Kostrem.): Effect of Antioxidants and 2,4-dichlorophenoxyacetic Acid Fitriani, Adistina; Arifin, Yudi Firmanul; Hatta, Gusti Muhammad; Wahdah, Raihani
Biota Vol 11 No 1 (2025): Jurna Biota 2025
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v11i1.22190

Abstract

The bark of tandui (Mangifera rufocostata Kostrem.) is commonly used as a medicine for diabetes. Regeneration of this plant is difficult, and continuous harvesting of the bark leads to a decrease in the plant population. The purpose of this research is to apply tissue culture techniques for the propagation of tandui. Different antioxidants (KNO3, polyvinylpyrrolidone (PVP), Murashige and Skoog (MS) media + PVP, and MS + ascorbic acid) and varying concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.8, 1.0, 1.2, and 1.4 mg L–1) were tested for embryogenic tandui. The application of KNO3 and PVP suppressed the formation of browning in the media and explant of tandui. Soaking the explants in KNO3 and PVP reduce browning to 35% and 20%, respectively. The results also showed that increasing the concentration of 2,4-D enhanced the percentage of embryogenic tandui. Supplementing the media with 1.4 mg L–1 2,4-D resulted in 90% of embryogenic tandui. This study demonstrates that pre-soaking explants in antioxidants significantly reduces media browning, and supplementation of MS media with 2,4-D enhance embryogenic process. Thus, micropropagation of tandui could be achieved on a commercial basis.
Co-Authors Abdul Hadi Abdurrachman Abdurrachman Adela Ayu Ningtias Adi Rahmadi Adi Rahmadi Ahmad Jauhari Ahmad Yamani Akhmad Yamani Andini Dyah Hernanda Arfa Agustina Rezekiah Astrid Cahyani Awaliah Awaliah Badaruddin Bara Sukma Fajar Basir Achmad Basir Basir Basir Basir Basir, Basir Damaris Payung Damaris Payung Dina Naemah Dina Naemah Dina Naemah Dina Naemah Dina Naemah Dina Naemah Dina Naemah Eka Sapitri Emmy Winarni Eny Dwi Pujawati Eny Dwi Pujawati Eva Prihatiningtyas Fachri Rahmadani Pratama Faisal Anwar Fakhrur Razie Fatriani Fatriani Gusti Muhammad Hatta Gusti Muhammad Hatta Gusti Muhammad Hatta Hadi, Wirawan Noor Hamdani Fauzi Heri Kurniawan Hidayat, Akhmad Noor Ihsan Noor Indy Nur Husna Junaedi Junaedi Kamarul Asrar Kristyanto, Sigit Lefi, Elsa Lenia M Aldy Rahmat Mahrus Ariyadi Mahrus Aryadi Mahrus Aryadi Maulana Khalid Riefani Mega Aulia Rahmayanti Mochamad Arief Soendjoto Muhlisah Muhlisah Nisa Nurul Isnaeni Sutisna Noormela Racmawati NormeIa Rachmawati Normela Rachmawati Normela Rachmawati Purnomo Purnomo Qadar Zailani Arifin Rachmawati, Normaela Rahmat, Muhammad Aldy Raihani Wahdah Richardo Adrian Naibaho Risnaniah Husna Riza Febriani Rodinah Rodinah Saiful Rahmadani Salwiyyah Salwiyyah Sigit Kristyanto Sirul Hayati Siti Hamidah Siti Qomariah Sudin Panjaitan Sudin Panjaitan Panjaitan Susilawati Susilawati Syafa’atul Aulia Zeni Syahid, Yulianto Tri Wahyuni Trisnu Satriadi Wahyu Saputra Wahyuni Ilham Wildani Syahri Ramadhan Yasinta Nur Shiba Yuda Agus Pratama Yudi Firmanul Arifin Yulianto Syahid Yusanto Nugroho