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Studi Karakter Morfologi dan Fisiologi Bibit Pisang Kepok Tanjung Daun Sempit dan Daun Normal dari Kultur Jaringan Bambang Hariyanto; Irfan Suliansyah; Yusniwati Yusniwati; Agus Sutanto
JURNAL TRITON Vol 16 No 1 (2025): JURNAL TRITON
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/jt.v16i1.1252

Abstract

Untuk mengetahui karakter tanaman pisang dapat dilakukan dengan cara mengamati karakteristik sifat morfologi tanaman. Pisang Kepok Tanjung dari kultur jaringan memiliki karakter daun yang berbeda yaitu daun sempit dan normal. Pengetahuan tentang studi karakter morfologi dan fisiologi bibit pisang Kepok Tanjung daun sempit dan daun normal dari kultur jaringan belum banyak diketahui. Penelitian yang bertujuan untuk mengetahui karakter morfologi dan fisiologi bibit pisang Kepok Tanjung daun sempit dan daun normal dari kultur jaringan dilakukan di Solok, Sumatera Barat bulan Maret hingga September 2023. Penelitian dilakukan secara deskriptif dengan mengkarakterisasi morfologi dan membandingkan parameter pertumbuhan serta karakter fisiologi dari total 180 bibit tanaman pisang Kepok Tanjung berdaun sempit dan normal dari kultur jaringan. Hasil penelitian menunjukkan adanya perbedaan pertumbuhan dan karakter morfologi bibit pisang Kepok Tanjung daun normal dan daun sempit dari kultur jaringan. Bibit pisang Kepok Tanjung daun normal menghasilkan tinggi tanaman, jumlah daun, diameter batang dan lebar daun yang lebih tinggi dan berbeda nyata dengan bibit pisang Kepok Tanjung berdaun sempit akan tetapi tidak berbeda nyata untuk parameter panjang daun. Dari 33 karakter morfologi (batang, daun dan akar) yang diamati terdapat 28 karakter morfologi yang sama dan 5 karakter morfologi yang berbeda. Untuk parameter karakter fisiologi yaitu jumlah stomata dan kerapatan stomata tidak menunjukkan adanya perbedaan yang nyata.
PENINGKATAN EFISIENSI PENGGUNAAN LAHAN KELOMPOK TANI SUNGKAI PERMAI MELALUI TUMPANG SARI JAGUNG DAN KACANG TANAH DAN PENGENALAN CENDAWAN MIKORIZA ARBUSKULA (CMA) Yusniwati Yusniwati; Nilla Kristina
BULETIN ILMIAH NAGARI MEMBANGUN Vol. 2 No. 3 (2019)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v2i3.105

Abstract

The Sungkai Permai Lambung Bukik Farmer Group, Padang City, generally cultivates in monoculture, with limited productive land. The land is planted with rice, secondary crops such as corn, and some peanuts. In the past two years, corn farmers often experience crop failures, especially during the rainy season, where the sun shines less due to global climate change. Therefore, diversifying commodities on limited land so that land efficiency increases are significant to prepare the next source of seeds. To support increased land use efficiency, optimal soil conditions are needed. Utilization of Arbuscular Mycorrhizal fungi can improve soil fertility and is expected to become an alternative technology in plant cultivation. Science and technology activities based on the Study Program and the Assisted Nagari (IbPSNB) were carried out in groups of Permai Sungkai Farmers in the form of 1) Counseling on the pattern of corn-peanut intercropping cultivation, and how to make CMA spore cultures, 2). The demonstration plot for corn-peanut intercropping cultivation. Through this activity, farmers finally understood the benefits and patterns of corn-peanut intercropping. Farmers understand the role and method of making CMA spore cultures. Several farmers cultivated corn-peanut intercropping. Two farmers at the demonstration plot managed to harvest corn and peanuts at the same time. Other farmers have failed to harvest corn due to pest attacks but have succeeded in harvesting peanuts so that the losses experienced by farmers are reduced, and they still have stock of seeds for the next planting season.
Effect of Ultraviolet-C Irradiation on Morphological Character Changes in Patchouli (Pogostemon cablin Benth.) Haryanti, Sindi; Yusniwati, Yusniwati; Gustian, Gustian
Journal of Applied Agricultural Science and Technology Vol. 8 No. 3 (2024): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v8i3.284

Abstract

Patchouli (Pogostemon cablin Benth.) is among the top 20 essential oil-producing plants traded in the global market. This plant shows narrow genetic diversity due to non-flowering and seed production, increasing the challenges in obtaining new varieties through crossbreeding. Therefore, this research aimed to apply mutation method using ultraviolet-C irradiation combined with tissue culture to broaden the genetic diversity of patchouli. The potential of UV-C irradiation and the morphological changes occurring in the plant were explored from October to December 2023 at the Tissue Culture Laboratory, Andalas University, Padang. In addition, the experiment was arranged in a Randomized Complete Block Design (RCBD) with 10 treatment levels and 3 groups. The treatments included UV-C irradiation doses of 0 (wild type), 30, 60, 90, 120, 150, 180, 210, 240, and 270 minutes at a distance of 30 cm. Data for each patchouli plant per observation variable were presented as mean values, variance, and standard deviation and analyzed using an unpaired t-test. The results showed that exposure to ultraviolet-C radiation impacted several morphological characteristics, such as chlorosis, delayed bud development, increased bud and leaf count, as well as faster bud growth. This phenomenon shows the potential of ultraviolet-C radiation as a physical mutagenic agent.
Eksplorasi dan Karakterisasi Morfologi Tanaman Jengkol (Pithecellobium jiringa) di Kabupaten Agam Sumatera Barat Nissa, Ayu Khairun; Yusniwati, Yusniwati; Zainal, Aprizal
JURNAL AGROTEKNOLOGI UNIVERSITAS ANDALAS Vol 6 No 1 (2024)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jagur.6.1.9-16.2024

Abstract

Shallot (Allium ascolanicum L.) is one of the horticultural crops that is widely consumed by humans. This study aims to obtain interaction the type of mulch and doses of chicken manure on the growth and yield of shallots. This Research was conducted at Panai Pasir Talang , Muara Labuh, South Solok Regency with a height of ± 430 meters above sea level, in December 2018 to February 2019. The research method is an experiments used factorial complete randomized design with two factors. Factors I is the type of mulch (black plastic mulch, silver plastic mulch, rice straw mulch) and factors II is chicken manure (10 tons / ha, 20 tons / ha and 30 tons / ha).. The results showed that there was no interaction between the use of several types of mulch with chicken manure doses on the growth and yield of shallots. The use of black plastic mulch yielded the best results on fresh tuber weight per plot and per hectare of onion namely 1,643 kg / plot and 11.74 tons / ha. The dose of chicken manure of 20 tons / ha gave the best influence on plant height, number of leaves, fresh tuber weight per clump, per plot and per hectare as well as wind dry weight per clump, per plot and per hectare of onion.
Conservation Comprehensive Approach: Study on Exploration, Habitat Analysis, Propagation, and Reintroduction of the Indonesian Endemic Endangered Titan Arum (Amorphophallus titanum Becc.) Setiawan, Ryan Budi; Yusniwati, Yusniwati; Rahma Wati, Dela; Purnama Sari, Indah; Rahmah, Siti; Haryanti, Sindi; Arief Laksana Bosma, Pandu
Jurnal Manajemen Hutan Tropika Vol. 31 No. 3 (2025)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7226/jtfm.31.3.261

Abstract

Titan arum (Amorphophallus titanum Becc.) is an endemic species of Indonesia that is found naturally only in Sumatra and is classified as endangered, with an estimated 303 mature individuals remaining in their natural habitat. The population is decreasing due to deforestation, tuber exploitation, long flowering times, and protandry. Therefore, both in-situ and ex-situ conservation programs are necessary to preserve this species. The research aims to explore natural populations, analyze habitat conditions, develop propagation methods, and conduct reintroduction efforts to support the conservation of A. titanum. This study was conducted from 2023 to 2024 in Palupuah, Agam Regency, the Agriculture Faculty of Universitas Andalas, and several locations for the reintroduction of A. titanum. The research found that A. titanum habitat consists of forests rich in humus and litter, as well as banana cultivation areas. During the exploration, 7 individuals were identified: one in dormancy, one fruiting, and 5 in the vegetative phase. Seed germination experiments categorized seeds into seven classes based on their weight. The most dominant seed class weighed between 2.9 g and 3.2 g, comprising 38 seeds or 26.9% of the total. The germination rate and seedling growth were high, as all seeds exhibited 100% and produced seedlings of relatively uniform size. Propagation was carried out using in vitro culture techniques with petiole explants. The results showed that 2 mg L-1 benzyl aminopurine (BAP) successfully induced callus formation with a 100% induction rate, while a concentration of 1.5 mg L-1 achieved the highest shoot induction rate at 58.3%, with an average of 1.3 shoots and 7.9 roots per explant. The propagated seedlings were subsequently replanted in several locations for reintroduction activities.
Effect of the Combination of the Growth Regulators and Putrescine on the Somatic Embryogenesis of Wheat (Triticum Aestivum L.) on Some Types of Explants Yusniwati, Yusniwati; Setiawan, Ryan Budi
Journal of Applied Agricultural Science and Technology Vol. 7 No. 4 (2023): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v7i4.158

Abstract

Plant breeding programs need to be carried out in order to improve the genetics of wheat that is able to adapt to tropical environments through hybridization, mutation induction, tissue culture, and genetic transformation.  In vitro culture through somatic embryogenesis pathways plays an important role in genetic improvement and its integration with other breeding programs can positively affect the improvement of wheat quality, quantity, and development in Indonesia. The purpose of this study was to obtain an embryogenic callus induction method from the Dewata variety using five different types of explants, namely mature Seeds, immature embryos, immature seeds, leaf,  stem, and to obtain combination of plant growth regulators and putrescine on somatic embryogenesis of wheat.   The experimental design was prepared based on a complete randomized design with a combination treatment of embryogenic callus induction media consisting of 9 levels, namely: 1 ppm 2.4- D, 1 ppm 2.4-D + 1 ppm Picloram, ppm 2.4-D + 1 mg / L Picloram, 1 ppm 2.4-D + 10-4  M Putrescine, 1 ppm 2.4-D + 1 ppm Picloram +10-4  M Putrescine, 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine, 1 ppm 2.4 D + 10-3 M Putrescine, 1 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine, 2 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine. The results showed that the media used was able to induce embryogenic callus using mature seed and immature embryo, but  immature seed and leaf were not able to produce embryogenic callus. The best media  that produced the highest percentage of embryogenic callus was 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine with as much as 85.9% in young embryo explants.
Effect of Concentration and Time of Giving Paclobutrazol on Lumbu Hijau-Garlic Seedling Bulbs Kristina, Nilla; Gustian, Gustian; Yusniwati, Yusniwati; obel, Obel; Khairunnisa, Nadia
AGRIUM: Jurnal Ilmu Pertanian Vol 27, No 1 (2024)
Publisher : UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/agrium.v27i1.17643

Abstract

The farmer's interest in garlic planting is low because the yield is unsatisfied. One reason is the use of small cloves. Giving paclobutrazol is expected to increase the size of garlic cloves and bulbs. This research aims to determine the interaction between concentration and time of giving paclobutrazol, which produces the best size for cloves and bulbs. The research is a factorial, Completely Randomized Design. The first factor is the concentration of paclobutrazol:250 mg.L-1 and 500 mg.L-1 of water. The second factor is the time of applying paclobutrazol: 6 and 10, 8 and 12, 10 and 14 weeks after planting (w.a.p). Data were analyzed using variance, which will be continued with DNMRT at the 5% level. The results showed that giving paclobutrazol at 10 and 14 w.a.p resulted in the highest apparent stem diameter and yield, but even so, the weight of the largest and the smallest cloves was the lowest. Meanwhile, giving paclobutrazol at 6 and 10 w.a.p resulted in the highest weight of the largest cloves 1.95 g and the highest of the smallest cloves 0.55 g, even though the apparent stem diameter and yield were the lowest. Treatment of 250 mg.L-1 paclobutrazol resulted in the highest weight of the largest cloves 1.80 g.
Protein Content Analysis of Protease Enzymes Extracted from Ficus Species and Papaya Using Qubit Fluorometer Ismed, Ismed; Yenrina, Rina; Hasbullah, Hasbullah; Syukri, Daimon; Yusniwati, Yusniwati
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13683

Abstract

This study aims to quantify the protein content in crude ficin enzymes obtained from the fruit and latex of various Ficus species and Papaya. This research has been encouraged by the considerable potential of these enzymes in different biotechnological applications, while data with regard to the protein content of these enzymes is limited. This study used a quantitative approach using qubit fluorometer to quantify the protein content in crude enzymes derived from the fruit and latex of these plants. Based on results, the protein content in crude ficin enzymes, two types of research obtained from Ficus aurata (Miq.) fruit was 0.92µg/ml and from papaya fruit as 3.16 µg/ml. For latex, it was observed that the protein content in crude ficin enzymes was determined as 6.14 µg/ml for Ficus aurata (Miq.), 2.58 µg/ml for Ficus racemosa L., and 5,27 µg/ml for Ficus padana Burm.f.. The protein content from papaya latex was 15,98 µg/ml. These findings show the differences in the protein content of the enzymes obtained from different species of Ficus and Papaya as a basis for further study on using these enzymes for biotechnological purposes.
Pemanfaatan Kompos Jerami Padi dalam Pengembangan Bawang Merah di Ultisol Dataran Rendah Kota Padang Nilla Kristina; Yusniwati, Yusniwati; Benni Satria; Elara Resigia; Aries Kusumawati
ABDIKAN: Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi Vol. 2 No. 4 (2023): November 2023
Publisher : Yayasan Literasi Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55123/abdikan.v2i4.2801

Abstract

Shallots are a high-value horticultural commodity. But so far, they have been mostly developed in the highlands. Shallots are plants that like areas with hotter air temperatures, so they are suitable for cultivation in the lowlands. Still, the soil that is widely available in the lowlands of the city of Padang is Ultisol. Ultisol contains high clay, so the soil becomes dense, the available nutrients are low, and the ground has difficulty passing water. Using Ultisol as a land for planting shallots, it requires using organic fertilizer in large doses. One of the agricultural waste products that is available and has yet to be widely used in Lambung Bukik Village is rice straw. Rice straw is a waste that is difficult to decompose. Therefore, it is necessary to use a decomposer to get compost faster. Training on making rice straw compost and making demonstration plots was carried out in Lambung Bukik, Padang City, to support the development of shallot cultivation on lowland Ultisol. Farmers are very enthusiastic about learning to cultivate shallots and making organic fertilizer from rice straw.
Tamarillo (Solanum Betaceum) Application In Food Products Rahmi Holinesti; Yusniwati Yusniwati; Tuty Anggraini; Daimon Syukri
Jurnal Pendidikan Tata Boga dan Teknologi Vol 6, No 2 (2025): Jurnal Pendidikan Tata Boga dan Teknologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/jptbt.v6i2.26865

Abstract

Tamarillo (Solanum betaceum), a fruit native to South America, thrives in the highlands of Indonesia and is rich in bioactive compounds with significant potential for functional food development. This study employs a comprehensive literature review of 108 indexed national and international journals to explore the application of tamarillo in food products. Despite its nutritional benefits—such as high vitamin C, antioxidants, and dietary fiber—tamarillo remains underutilized in culinary applications. Current products incorporating tamarillo include syrup, ice cream, jam, soft candy, jelly, steamed bread, cheese, and yogurt. These products leverage tamarillo’s bioactive properties, such as its antioxidant and anti-inflammatory effects, which contribute to health benefits like improved digestion, immune support, and chronic disease prevention. However, further research is needed to expand its applications and optimize its potential in functional foods
Co-Authors . Reflinaldon Afrizal Nofri Agus Sutanto Agus Sutanto Agus Sutanto Alfi Asben Anwar Kasim Aprissilia Taifani Galuh Utomo Aprizal Zainal Arief Laksana Bosma, Pandu Aries Kusumawati Aswaldi Anwar Auzar Syarif Baiturrahman, Atzim Bambang Hariyanto Bambang Hariyanto Bambang Hariyanto Benni Satria Bosma, Pandu Arif Laksana Budi, Rahmad Setia Daimon Syukri Damayanti, Elok Deivy Andhika Permata Dendy Tri Dharma Putra Denny Yulfa Dewi Rezki Dian Sukma, Dian Etti Swasti Fadilla Arishadea Fauzan Abid Hanif Nasution Febriani Febriani Gusti Marlina Gustian Gustian Gustian Hafnes Wahyuni Haliatur Rahma, Haliatur Handayani, Mellyyana Haryanti, Sindi Hasbullah Hasbullah Hasbullah Hasbullah Hayati, PK Dewi Hidrayani Hidrayani Illahi, Ayu Kurnia Indri Yanil Vajri Indri Yanil Vajri Indri Yanil Vajri Irfan Suliansyah Ismed Ismed Jumsu Trisno Khairunnisa, Nadia KRISTINA, NILLA Lily Syukriani Magdalena Saragih Magdalena Saragih Saragih Mismawarni Srima Ningsih Nalwida Rozen Nanda, Alifma Rahimmi Neswati Neswati Netti Herawati Nissa, Ayu Khairun Nofialdi, Nofialdi Noflindawati Noflindawati Nofrita Sandi Nova Reskhi Firdaus Novizar Nazir Nurbailis Obel, Obel Prana Dipa Tiarani Prana Dipa Tiarani Purnama Sari, Indah Putri Salsa Afifah Rahma Wati, Dela Rahmi Holinesti Rahmi, Alfazlul Reni Mayerni Rina Yenrina Roza Yunita Ryan Budi Setiawan Sahroni Ridoi Saragih, Magdalena Sembiring, Rinawati Siska Efendi Siswahyudianto Sobrizal Sobrizal Sri Wahyuni Syahbanuari Sitompul Syahputra, Agil Syukri Arif Trizelia Tuty Anggraini Winarto Winarto Yaherwandi Zahlul Ikhsan Zurai Resti