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Isolation and characterization of phosphate-solubilizing bacteria in land managed with the dusung system Ohiwal, Morgan; Marasabessy, Rendy; Yunita, Melda; Sukmawati, Sukmawati; Qibtiyah, Mariyatul
JPBIO (Jurnal Pendidikan Biologi) Vol 10, No 2 (2025): November 2025
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v10i2.4821

Abstract

Soil microorganisms play a crucial role in nutrient cycling and soil fertility, particularly through their ability to solubilize insoluble phosphate compounds. This study aims to isolate and characterize phosphate-solubilizing bacteria (PSB) on land managed under the dusung agroforestry system. Soil samples were collected from five types of land use: forests, mixed orchards, clove hamlets, nutmeg orchards, and shrubs. PSB isolation was performed using Pikovskaya medium with the spread plate method, followed by characterization of colony morphology (shape, edge, color, elevation) and Gram staining. The results showed that the highest PSB population occurred in forest land (8.3 × 10? CFU/ml), followed by mixed orchards (6.2–6.7 × 10? CFU/ml), clove orchards (5.3–5.4 × 10? CFU/ml), nutmeg orchards (3.2–5.6 × 10? CFU/ml), and the lowest in shrubs (7.1 × 10³ CFU/ml). The characteristics of PSB varied, with isolates from forests and nutmeg orchards predominantly consisting of coccus-shaped Gram-positive bacteria, while those from mixed and clove orchards were dominated by bacillus-shaped Gram-positive bacteria. The population and diversity of PSBs were influenced by the availability of organic matter, soil pH, and land management practices. These findings highlight the ecological importance of the dusung system in sustaining soil fertility and promoting ecosystem resilience.
Analysis of dl--tocopherol as antioxidant on malondialdehyde level in pediatric patients with -thalassemia major Rismayanti, Levana; Yulistiani, Yulistiani; Andarsini, Mia Ratwita; Qibtiyah, Mariyatul
Folia Medica Indonesiana Vol. 53, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

Thalassemia is a hereditary form of anemia that affects the synthesis of hemoglobin. The management of therapy in patients with b-thalassemia major which patients should receive continuous blood transfusions and increased iron absorption from the digestive tract causes excess iron in the body. This will lead to an increase of free iron level that triggers Radical Oxygen Species (ROS). Increased level of ROS can initiate lipid peroxidation which used as an indicator of oxidative stress in cells and tissues and produce reactive carbonyl, mainly malondialdehyde (MDA). Thus, MDA measurement is widely used as an indicator of lipid peroxidation. On the other hand, the risk of oxidative damage can be reduced by antioxidant, one of them is Vitamin E that is a fat-soluble vitamin with high potential antioxidant. The objective of this study was to analyze the effect of the dl-a-tocopherol (Vitamin E) administration on decrease of MDA serum level on pediatric patients with b-thalassemia major. This was a longitudinal observational study design for one group without comparison was conducted to examine the use of vitamin E to decreased MDA serum level on children patients with b-thalassemia major. The inclusion criteria were patients who rely on blood transfusions, patients who received only one type of iron chelating agents during the study period, the clinical condition is stable, agrees, and has completed the informed consent. In the course of the study of 21 patients there were variations in patient compliance in taking vitamin E tablet dosage 200 IU once-daily for one month: only 11 out of 21 patients consumed 30 tablets of vitamin E 200 IU (total dose of 6000 IU) in the 1-month study, and only data from those 11 samples will be analysed further. MDA serum level was measured pre- and post-administration of vitamin E and patient's characteristics of subjects was obtained for additional information. Pre-administration of vitamin E, serum level of MDA was 1239.4 ± 502.55 ng/mL with a range of 216.95 to 2297.3 ng/mL, whereas in the group post administration of vitamin E, MDA serum level was 786.49 ± 704.88 ng/mL with a range of 6.5380 to 1958.6 ng/mL. In conclusion, there was no significant difference in MDA serum level in the group pre- and post- administration of vitamin E (p = 0.15).
Analysis of induction phase glucocorticoid use on adrenal suppression in pediatric patients with acute lymphoblastic leukemia Simbolon, Octaviana; Yulistiani, Yulistiani; Ugrasena, I DG; Qibtiyah, Mariyatul
Folia Medica Indonesiana Vol. 52, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

Glucocorticoids play an important role in the treatment of acute lymphoblastic leukemia (ALL). However, supraphysiological doses may cause suppression of the adrenal. Adrenal suppression resulting in reduced cortisol response may cause an inadequate host defence against infections, which remains a cause of morbidity and mortality in children with ALL. The occurrence of adrenal suppression before and after glucocorticoid therapy for childhood ALL is unclear. The aim of this study is to analysis the effect of glucocorticoid on cortisol levels during induction phase chemotherapy in children with acute lymphoblastic leukemia. A cross-sectional, observational prospective study was conducted to determine the effect of glucocorticoid on cortisol levels in children with acute lymphoblastic leukemia. Patients who met inclusion criteria were given dexamethasone or prednisone therapy for 49 days according to the 2013 Indonesian Chemotherapy ALL Protocol. Cortisol levels were measured on days 0, 14, 28, 42 and 56 of induction phase chemotherapy. There were 24 children, among 31 children recruited, who suffered from acute lymphoblastic leukemia. Before treatment, the means of cortisol levels were 228.95 ng/ml in standard risk group (prednisone) and 199.67 ng/ml in high risk group (dexamethasone). In standard risk group, the adrenal suppression occurs at about day 56. There was a significant decrement of cortisol levels in high risk group in days 14, 28, 42 against days 0 of induction phase (p=0.001). Both groups displayed different peak cortisol levels after 6 week of induction phase (p=0.028). Dexamethasone resulted in lower cortisol levels than prednisone during induction phase chemotherapy in children with acute lymphoblastic leukemia.
Analysis of High Dose and Long-Term Prednisone Therapy on Trap 5B Level Change in Children with Steroid Sensitive Nephrotic Syndrome Setyani, Dessy Surya; Qibtiyah, Mariyatul; Asmaningsih, Ninik; Yulistiani, Yulistiani
Folia Medica Indonesiana Vol. 54, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Nephrotic syndrome is a condition which is characterized by protein leakage from the blood to the urine through glomeruli. It leads to hypoproteinemia and generalised oedema. Patients with nephrotic syndrome need high dose and long term glucocorticoid such as prednisone. High dose and long term glucocorticoid can increase bone resorption. Biological marker is a valuable tool to evaluate efficacy of therapy. TRAP 5B is a sensitive biological marker for bone resorption because it reflects the number of osteoclasts. TRAP 5B is not affected by renal dysfunction and food. It also has a low diurnal variation than other bone resorption marker. The aim of this study was to analyze the changes of TRAP 5B levels at induction and alternate phase in children with steroid sensitive nephrotic syndrome. This observational prospective study was conducted from May to October 2016. Venous blood samples obtained at 08.00-10.00 am. TRAP5B levels were measured before and after induction phase and after alternate phase using ELISA. Fifteen patients were included in this study (60% boys). Majority of their age was 6 - <12 years and 40% were dependent steroid NS. TRAP 5B serum levels in induction phase increased by 37.41%±56.22%. In alternate phase, TRAP 5B serum levels increased by 28.75%±66.55% compared to the induction phase. However, the level change of both phases were not significant. As a conclusion, TRAP 5B levels increased in induction and alternate phase after high dose and long-term prednisone treatment in nephrotic syndrome.
Pandangan Tokoh Agama Terhadap Sistem Distribusi Zakat Fitrah Model Tukar Antar Muzakki (Studi di Masjid An-Nur Dusun Takeran Desa Ngijo Kecamatan Karangploso Kabupaten Malang) Qibtiyah, Mariyatul
Sakina: Journal of Family Studies Vol 3 No 1 (2019): Sakina: Journal of Family Studies
Publisher : Islamic Family Law Study Program, Sharia Faculty, Universitas Islam Negeri Maulana Malik Ibrahim Malang

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Abstract

Artikel ini bertujuan untuk mengetahui dan mendeskrisikan model distribusi tukar zakat yang dilakukan oleh masjid An-Nur, menganalisis dengan pendapat tokoh agama dari Nahdlatul Ulama, Muhammadiyah dan Majelis Ulama Indonesia serta mengetahui persamaan dan perbedaan pendapat tokoh tersebut. Artikel ini merupakan jenis penelitian empiri, dengan menggunakan pendekatan deskriptif kualitatif. Data yang diperoleh berupa data primer dari wawancara kepada panitia penerima zakat fitrah Masjid An-Nur dan tokoh Nahdlatul Ulama, Muhammadiyah dan Majelis Ulama Indonesia. Dan dilengkapi data sekunder berupa dokumentasi data yang bersumber dari buku dan berkas lembaga. Hasil penelitian ini adalah Pertama, menurut keseluruhan tokoh agama berpendapat, pendistribusian zakat fitrah secara tukar diperbolehkan selama tidak menyalahi aturan fikih. Mustahiq harus dipilih sesuai kategori 8 asnaf. Jika terdapat muzakki yang berstatus sebagai mustahiq, maka pendistribusiannya harus dilakukan secara teliti. Kedua, Persamaan padangan tokoh agama adalah Keseluruhan tokoh agama memperbolehkan melakukan distribusi tukar zakat. Perbedaannya, pandangan tokoh Muhammadiyah muzakki yang mengeluarkan zakat tidak dapat disebut sebagai mustahiq sehingga zakat tidak kembali kepada muzakki.