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All Journal PROSIDING SEMINAR NASIONAL Jurnal Kesehatan Masyarakat Indonesia JURNAL LITBANG JURNAL KESEHATAN FIKkeS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Biomedika Prosiding Seminar Nasional Unimus
Sri Darmawati
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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DAYA HAMBAT DAUN ASAM JAWA (Tamarindus indica) TERHADAP PERTUMBUHAN Salmonella typhi PENYEBAB DEMAM TIFOID Dini Puspodewi; Sri Darmawati; Endang Triwahyuni Maharani
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Student Paper Presentation The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tifoid fever is endemic disease who caused by Salmonella typhi bacteria and still to be seriously health problem in Indonesia. The treatment still use antibiotic who can cause resistant if uncontrol using. Tamarind (Tamarindus indica) is plant who can use as medicine. The leaves contain flavonoid, tannin, and saponin who can function as antibacteria. The purpose of this research to know inhibition of tamarind leaves to Salmonella typhi who caused tifoid fever in 25% consentration. This research using old tamrind leaves from Rembang. The leaves was drying and powdering, then soxhletation with eter, extraction with maceration and infused method using distiled water with 25% concentration, then freeze dying and make 1, 2, 3, 4, and 5 mg/25 μL concentration per disc in a row. antibacteri tested with Kirby bauer method. The result of this experiment showing all of variance concentrations at disc can inhibit Salmonella typhi BA07.4 in maceration method as well as in infused method in 25% concentration of tamarind leaves. 5 mg/25 μL concentration has large antibacterial essence showed with existence large zone of inhibition 11,5 mm in maceration method and 14 mm in infused method. infused method of 25% tamarind leaves is more antibacterial essence than maceration method.Keyword : Tamarind leaves, Salmonella typhi, Tifoid fever
ISOLASI BAKTERI PENGHASIL ENZIM PROTEASE BACILLUS MEGATERIUM IROD3 DARI ONCOM MERAH PASCA FERMENTASI 72 JAM Dhea Ayu Lestari; Sakti Imam Muchlissin; Ana Hidayanti Mukaromah; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Protease  enzyme  has  function  to  hydrolyze  peptide  bonds  in  proteins  into simpler molecules to digest by the body which important to food industry. One of effort to increase the production of protease enzymes is looking for new sources of protease particularly from bacterial groups. The purpose of this study was to obtain an isolate of protease-producing bacteria found on post- fermentation oncom 72 hours, and to identify the protease-producing bacteria based on the analysis of 16S rRNA gene. Isolation and purification process of bacterial colony was carried out on Nutrient Agar medium with spread technique, production test of protease enzyme was performed using Selective Skim Milk Agar. The process of Molecular identification process was carried out  through  analysis  of  16S  rRNA  gene  fragment  sequences  which  were amplified using Polymerase Chain Reaction (PCR) method, and continued by sequencing. The result of bacteria isolation was found one isolate which has proteolytic activity in Skim Milk Agar medium which has clear zone diameter of 78.00 mm. A similarity analysis based on the 16S rRNA gene sequence showed that IROD3 (Indonesian Red Oncom Day-3) has 99% similarity level with the 16S rRNA gene fragment of Bacillus megaterium strain CS17 (access code Genbank: MG430224.1). Keywords:  Molecular  identification,  proteolytic  bacteria,  16S  rRNA  gene, Bacillus megaterium
ANALISIS IMUNOGENISITAS PROTEIN 58 kDa Salmonella typhi Sri Darmawati; Syaiful Anwar
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2014: PROSIDING SEMINAR NASIONAL HASIL - HASIL PENELITIAN & PENGABDIAN
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tujuan penelitian untuk mengetahui  imunogenisitas protein 58 kDa dari Strain Salmonellatyphi BA 07.4 dan spesifisitas antibodi yang ditimbulkannya  terhadap protein sel bakteri bentukbatang Gram negatif anggota familia Enterobacteriaceae dari kultur darah pasien Widal positif(Escherichia coli, Enterobacter cloacae, Serratia marcescens, Klebsiella pneumoniae). Metode yang digunakan adalah isolasi total protein bakteri, separasi total protein dengan metode SDS-PAGE, isolasi protein 58kDa untuk imunisasi ayam, imunisasi ayam Loghman secara subkutan dilakukan 3 kali, isolasi antibodi poliklonal dari kuning telur ayam, dan imunoblotting.Hasilnya menunjukkan bahwa protein 58kDa dari Salmonella typhi BA 07.4 bersifat immunogenik, karena dapat menimbulkan terbentuknya antibodi,   tetapi tidak spesifik  karena selain mengenali protein 58kDa yang dimiliki oleh Salmonella typhi BA07.4, juga   bereaksidengan protein yang dimiliki oleh bakteri anggota familia Enterobacteriaceae yang lain(Escherichia coli, Enterobacter cloacae, Serratia marcescens, Klebsiella pneumoniae)Kata Kunci: Salmonella typhi, protein 58 kDa.
ISOLASI DAN IDENTIFIKASI MOLEKULER BAKTERI PENGHASIL ENZIM PROTEASE PSEUDOMONAS STUTZERI ISTD4 DARI TEMPE GEMBUS PASCA FERMENTASI 1 HARI Wa Ode Inayatul; Sakti Imam Muchlissin; Ana Hidayati Mukaromah; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Proteaseis  a group  of enzymes that  play  an important  role in  biochemical reactions, whichc a use protein break down. Protease is among main enzymes used in industry, which commercial value reach 60% of total enzymes world wide. This study a imed toisolat protease-producing bacterium found on tempe gembus in after 1-day post-fermentation and to identify the bacterial isolat obtained based on the analysis of its 16S rRNA gene. Isolati on and purification process wasd one using Nutrient Agar media with spread technique. The protease production test was carried out on skim milk agar medium. The molecular identification process was performed by analyzing sequence of 16S rRNA gene fragment of bacteria amplified using both forward primer F (F:5'- AGAGTTGATCCTGGCTCAG-3'), and reverse primer R (R:5'- GGTTACCTTGTTACGACTT-3') by Polymerase Chain Reaction (PCR) method. The amplified DNA from PCR was then sequenced. From the isolation process a bacterial strain that has a proteolytic activity based on observation of clear zone area with a diameter of 85 mm was obtained. From sequence alignment result using BLAST (Basic Local Alignment Search Tool) the fragment of 16S rRNA gene of strain ISTD1.4 obtained has similarity level of 98% with fragment of 16S ribosomal RNA gene of bacterium Pseudomonas stutzeri strain E141. In conclusion, strain ISTD1.4 is a potential protease-producing bacteria and is identified as Pseudomonas stutzeri ISTD4. Keywords: Bacterial isolation, molecular identification, proteolytic bacteria, 16S rRNA gene
ISOLASI BAKTERI PENGHASIL ENZIM PROTEASE BACILLUS AMYLOLIQUEFACIENS IROD2 PADA ONCOM MERAH PASCA FERMENTASI 48 JAM Dwi Pamaya; Sakti Imam Muchlissin; Endang Tri Wahyuni Maharani; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Proteolytic  bacteria  are  the  bacteria  capable  of  producing  extracellular protease enzymes, namely protein-breaking enzymes that are widely used in many industrial fields. This study aimed to isolate a proteolytic bacterium found on 48-h post-fermented oncom and molecular identification method. The initial isolation and purification process of the colony was carried out using Nutrient Agar medium. Selection of protease enzyme obtained by bacterial isolate was done on Milk Skim Agar medium. Identification process of the isolate was done through amplification of 16S rRNA gene using PCR, sequencing and analysis of gene sequences using BLAST program. From the isolation process  a bacterial isolate that has proteolytic by the ability to produce a clear zone of 82.00 on plate. The result of the 16S rRNA gene sequence analysis showed that the proteolytic bacterial isolate obtained in this study had a 98% homology level with 16S ribosomal RNA isolate of Bacillus amyloliquefaciens strain A1142 (Genbank access code: KTT722836.1). Based on the results of the molecular identification, the isolate was identified as Bacillus amyloliquefaciens strain IROD2  (IROD2 = Indonesia Red Oncom Day2). As conclusion, from 48-h post fermented red oncom, a protease producing bacterial strain molecularly identified as Bacillus amyloliquefaciens strain IROD2. Keywords: Moleculary was identified, Proteolitic bacteria, 16S rRNA gene
PROFIL PROTEIN DAGING KAMBING, KERBAU DAN SAPI YANG DIRENDAM LARUTAN JAHE BERBASIS SDS-PAGE Rieke Fadhila; Sri Darmawati
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Ginger contains protease enzyme and that is Zingibain which can hydrolyze proteins so it can soften meat. The aim of this study is to analyze protein profiles in goat, buffalo and cow meats before and after those meats are soaked with ginger solution in 4% v/v, 6% v/v, 8% v/v and 10% v/v concentrations for 30 minutes. Protein profile of meats was analyzed with SDS-PAGE method. The result of this study shows that control meats which are not soaked with ginger solution have many major protein bands while sample meats which have soaked with ginger solution have many minor protein bands. The result of this study shows thatsoaking meats with ginger solution can soften meats because zingibain enzyme in ginger solution can break peptide bonds in protein of meat so it forms micromolecules (minor bands). Ginger solution which is the best to soak goat, buffalo and cow meats is on 4% concentration because there are so many proteins in those meats. The higher ginger solution concentration is the more and more zingibain enzyme content are so the ability to hydrolize protein is higher marked with slackening major protein bands and increasing minor protein bands. Keywords: Meat, Ginger, Protein Profiles, SDS-PAGE
AKTIVITAS ANTIBAKTERI MADU TERHADAP BAKTERI MULTI DRUG RESISTANT Salmonella typhi DAN METHICILLIN-RESISTANT Staphylococcus aureus Rahma Asriani Panjaitan; Sri Darmawati; Muhammad Evy Prastiyanto
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

This study aimed to analyze the antibacterial activity of Palm oil Honey against Multi  Drug  Resistant  Salmonella  typhi  and  Methicillin-Resistant Staphylococcus aureus by concentration 50%, 60%, 70%, 80%, 90% and 100%. This  research  was  an  experimental  test  with  Posttest  Only  Control  Group Design in an in vitro manner using well diffusion method techniques. The well diffusion method used MHA which made by the well of 5 mm diameter and inserted 200 µ L sample then incubated 35 ± 20C for 24 hours. The results of the study showed the antibacterial activity of Palm oil Honey with zone inhibition against MDR S.typhi 11.4 mm (concentration 90%), 13.4 mm (concentration 100%) and against MRSA 11.7 mm (concentration 100%). The results of zone inhibition of Palm oil honey with concentration 100% against MDR S.typhi was larger than the  zone inhibition  against MRSA. The palm oil honey inhibition zone  against  S.typhi  MDR  bacteria  compared  with  sulfamethoxazole  (SXT) antibiotics with 25 mm inhibition zones was included in the intermediate category and the palm oil honey inhibition zone against MRSA bacteria compared with Tetracycline (TE) antibiotics with 23 mm inhibition zone was included in resistant category. Keywords: Antibacterial Activity, MDR S. typhi, MRSA, Honey, Inhibition Zone
IDENTIFIKASI DAN HITUNG JUMLAH BAKTERI KONTAMINAN PADA LALAT Masca domestica BERDASARKAN LOKASI PENANGKAPAN DI RUMAH SAKIT BHAYANGKARA SEMARANG Sri Darmawati; Sayono -; Misno Sudarmadi
Jurnal Kesehatan Masyarakat Indonesia Volume 2. No. 2. Tahun 2005
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Latar belakang: Lalat, khususnya lalat rumah (Musca domestica) merupakan salah satu vektor mekanis beberapa jenis penyakit. Aktivitas berkembang biak pada sampah dan mencari makan pada makanan manusia berpotensi menimbulkan kontaminasi bakteri pada makanan dan minuman. Di lingkungan rumah sakit, hal ini sangatpenting untuk diperhatikan. Tujuan: mengidentifikasi bakteri dan menghitung jumlah bakteri kontaminan yang terdapat pada tubuh lalat.Musca domesetica. Metode: Jenis penelitian ekplanatori, dengan rancangan cross sectional dan pendekatan observasional. Populasi penelitian adalah lalat Musca domestica yang berada di lingkungan rumah sakit Bhayangkara Semarang. Sampel diambil secara accidental (kebetulan). Lalat ditangkap dengan jaring penangkap lalat. Variabel bebas, yaitu tempat (terdiri dari 3 lokasi), dan variabel terikat adalahjumlah bakteri kontaminan pada tubuh. Jumlah bakteri dihitung dengan metod Standart Plate Count (SPC). Data diolah dengan program komputer dan dianalisis secara diskriptif dan analitik. Analisis analitik menggunakan uji Analisa Varians Satu Jalan (One Way Anova). Hasil; Hasil identifikasi ditemukan, lima jenis bakteri kontaminan adalah Providencia rettgeri, Providencia stuartii, Enterobacter aerogenes, Citrobacter freundii, dan Bacillus sp., rerata jumlah bakteri pada lalat yang ditangkap di dapur adalah 1,85 x 104 sel batkteri/ekor, di TPS 7,4 x  103sel bakteri/ekor dan di bangzaal perqwatan 1,93 x 103 sel bakteri/ekor, hasil uji Anova menunjukkun nilai F = 1,142 dan p : 0,336. Simpulan: Ada lima jenis bakteri kontaminan yang ditemukan pada tubuh lalat di lingkungan rumah sakit Bhayangkara Semarang, tidak ditemukan bakteri Salmonella sp, dan tidak ada perbedaan jumlah bakteri secara signifikan menurut tempat penangkapan lalat.Kata kunci: Musca domestica, bakteri kontaminan, rumah sakit
ANALISIS PROTEIN PILLI Salmonella typhi lsolat RS. Kariadi Semarang DENGAN ELEKTROFORESIS SDS-PAGE Sri Darmawati; Ratih Haribi
JURNAL LITBANG Vol 2, No 3 (2005): Penelitian, Pengembangan, dan Pengabdian
Publisher : JURNAL LITBANG

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Abstract

Background : Salmonella typhi is bacteria which causes typhoid fever. Typhoid fever is severally serious, infectious decease and is endemic decease in Indonesia with relatively high frequency rate around 358-810/100.000 people each year This decease is usually spreading along with many kinds of decease which also have relatively high mortality rate, that is 1-5% of the sufferers (Punjabi, NH. , 2 004) . The infection process of bacteria into human body is signified with bacteria Cell adhering in mucosa intestinal surface. Pilli is structured by pilli protein consisting of Several sub units of pilli protein Objective: conducting pilli protein Salmonella typhi isolate Kariadi Hospital Semarang Analysis through electrophoreses SDS-PAGE, so that take amount of sub unit of pilli protein with each its molecule weight can be found out. Method : This research is conducted through three stages; firstly, bacteria cultivation with Biphasic media (BHI Agar and broth BHI ); secondly, pilli isolation with Ehara method (/956); Thirdly, sub unit of pilli protein separation with I 2% SDS-PAGE based on Lemmli method (1970). Result: There are four major sub units of protein in pilli, that is, 36kDa; 26,5 kDa; 22,2kDa; 18,6lrDq and there are also four minor sub units of protein pilli, that is, 116 kDa; 62,3kDa;45kDa;20,9kDa,andseveral other minor sub units of protein with every thin band. Keywords : Salmonella and Electrophoresis
KELAINAN FUNGSI HATI DAN GINJAL TIKUS PUTIH (Rattus norvegicus, L.) AKIBAT SUPLEMENTASI TAWAS DALAM PAKAN Ratih Haribi; Sri Darmawati; Tri Hartiti
JURNAL KESEHATAN Vol 2, No 2 (2009): Ilmu-Ilmu Kesehatan
Publisher : JURNAL KESEHATAN

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Abstract

Abstract Alum is used to improve the quality of food containing toxic heavy metal ions which can interfere with Aluminum enzymatic system, and tissue damage. Liver and kidney are the most used network is affected, because it is a detoxification organ. Liver and kidney damage can be detected by an enzyme concentration of SGOT, SGPT, Billirubin, Protein, Ureum and Creatinin in the blood This study aims to find out the effects of alum in a feed supplement for liver and kidney damage in a clinic conducted from May to Oclober 2007, at the Laboratory of the University Clinic Patologt Muhammadiyah Semarang. Sample studies of white rats (Rattus norvegicus, L.), aged 2 months with weight average of 200 grams. 0o/o dose treatment (without supplementation), 0.05%, 0.1%, 0.2%, 0.5%, 1% and 0% (without supplementation), and subsequent treatment with a dose of 2%, 3%, 4%, 5 % and 6% volum, who every day put into the stomach of rats l0 mL Clinical laboratory tests performed at the time before treatment (control), 4 weeks, 6 weeks and 8 weeks of exposure time. Examination AST and ALT with Ultra Violet Test methods, Total Billirubin with modifications Groff Jendrasik method, total protein Colorimetri method, U Berthelot method, Creatinin Jaffe method. Clinical chemistry tests showed that supplementation influence of alum on the concentration of Enzymes and other factors in the blood of mice associated with damage to liver and kidney tissue. Level of organ damage significant with alum in a feed supplement. The higher the concentration of alum disuplementasion and the longer exposure time resulted in damage to the liver and kidneys getting worse.
Co-Authors - Anawar S Ana Hidayanti Mukaromah Ana Hidayati Mukaromah Aprilia Indra Kartika Asri Rahmiati Aulia Harun Ayu Rahmawati Sulistyaningtyas Budi Santosa Deastika Widianingratri Defi Nurul Hayati Dhea Ayu Lestari Dini Puspodewi Dwi Pamaya Endang Tri Wahyuni Maharani Endang Triwahyuni Maharani Fandhi Adi Wardoyo Fatikhin - Haris Susena Idayanti . . Iin Inayatul Karomah Insannul Kurniasari Juni Nauli Laksono Trisnantoro Langkah Sembiring Masashi Kawaichi Meutia Srikandi Fitria Misno Sudarmadi Mudyawati Kamaruddin Muh. Amin Muhammad Evy Prastiyanto Nevi Kustia Nurasia Nurasia Nurrahman Nurrahman Nursaima Siregar Radna Safitri Ragil Saptaningtyas Rahma Asriani Panjaitan Ratih Haribi Rieke Fadhila Rinda Aulia Utami Rizka Ayu Wahyuni Rizka Yolanda Febiaocti Sakti Imam Muchlissin Sayono Sayono Sindi Setiawati Ali Utami Sri Dewi Haryati Sri Sinto Dewi Sri Suhartati Stalis Norma Ethica TRI HARTITI Ulfa Nurullita Vivi Yosafianti Pohan W T Artama Wa Ode Inayatul Wa Ode Jariah M Wayan T. Artama Widya Asmara Wildiani Wilson