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All Journal AGRIVITA, Journal of Agricultural Science Agrin : Jurnal Penelitian Pertanian AGRITROPICA : Journal of Agricultural Sciences Akta Agrosia JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Pada Masyarakat
Rustikawati Rustikawati
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Induksi Kalus dan Regenerasi Tanaman pada Kultur Antera Persilangan Padi Indica Varietas Lokal Bengkulu Herawati, Reny; Rustikawati, Rustikawati; Inoriyah, Entang
Akta Agrosia Vol 18 No 2 (2015)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Establishment of homozygous lines can be accelerated with anther culture technique that can produce pure lines in one generation. Formation of spontaneous double haploid plants in rice anther culture is very beneficial, because it does not need to be doubled haploid plants as material selection.This study aims to obtain a doubled haploid lines of upland rice is tolerant to drought and blast disease resistance. The main ingredient in this research is anther crosses of rice plants (F1), which consists of local varieties with selected lines of P1 (Sriwijaya x IR-148), P2 (Sriwijaya x IR-7858-1), P3 (Bugis x IR -148), and P4 (Bugis x IR-7858-1), callus induction media (N6), regeneration medium (MS). Anther culture of local varieties of rice indica/indica produced callus induction and plant regeneration are low, resulting in low efficiency of anther culture in providing green plants. The use of donor parents indica/j aponica which is responsive to anther culture into consideration for the material F1 crosses. Anther inoculation needs to be done in several stages so that the opportunity to obtain plantlets in sufficient quantities for material selection will be greater.
Potensi Produksi Enam Hibrida Jagung pada Ultisol di Provinsi Bengkulu Suprijono, Eko; Rustikawati, Rustikawati; Romeida, Atra; Gustian, Meko
Akta Agrosia Vol 18 No 2 (2015)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Ultisol is one of marginal land type quite extensively presence in Indonesia, including the province of Bengkulu. The use of varieties tolerance to acidity stress is the best manner to utilize of this type of land with relatively low cost and environmentally friendly. The objective of this research was to evaluate theyield potential of six maize hybrids developed to soil acidity tolerance. The experiment was conducted at the Experimental Farm Medan Baru, Kandang Limun Village, Muara Bangkahulu District, Bengkulu City, from August to November 2015. The experiment was arranged in a randomized completely block design with four replications. As the treatment were newly developed maize hybrids, namely CT5, CT8, CT9, CT13, CT14 and CT15. The variables measured were cob length, cob diameter, 100-seed weight and the estimated yield/ha. Qualitative variables supporting to corn yield were the cob position, seed color, seed type and seed row arrangement. The results indicated that the hybrid CT8 showed the highest estimated yield in Ultisol among hybrids evaluated, with the estimation of 6.5 tons per hectare. Qualitatively, theseeds of hybrid CT8 were a horse tooth type, pale yellow color, and stright line arrangement.
Penentuan Kadar Garam Kultur Hara untuk Seleksi Toleransi Salinitas pada Padi Lokal Bengkulu Rustikawati, Rustikawati; Simarmata, Marulak; Turmudi, Edhi; Herison, Catur
Akta Agrosia Vol 17 No 2 (2014)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Bengkulu province area elevating from 0 m to over 1000 m above sea level possesses high rice germplasm diversity. To obtain salinity controlling gene(s) from those germplasm, it is required to determine a suitableprotocol. This research was objected to determine NaCl concentration to select Bengkulu rice landraces. The standard most salinity tolerant genotype used in this research was ’Kuning’, a local variety usually grown on tidal area of Seluma Regency for generations. The growing media was Yoshida nutrient culture supplemented with a series of NaCl concentration of 0, 2000, 4000, 6000, 8000 or 10000 ppm. Observation was done every 2 days to find out the response pattern of rice seedling growth on different NaCl concentration. NaCl stress level was determined at 90% seedling dead (LC90) at the eighth day. The results showed that the increase of plantheight was deceased at concentration of 6000 ppm. Based on the mathematical equation generated from the percent mortality data distribution, the LC90 was at 3910 ppm NaCl concentration.
Performance of Fifteen F5 Pedigree Upland Rice Lines in Ultisol Luwih, Bagus Edi; Rustikawati, Rustikawati; Hindarto, Kanang Setyo
Akta Agrosia Vol 20 No 2 (2017)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

ABSTRACTRice (Oryza sativa L.) is a rice-producing crop that is the main food source for most of Indonesia's population. Although rice production increases but needs to be maintained. One of the efforts in maintaining rice production is by assembling superior paddy varieties that are adaptive to Ultisol soil. This study aims to compare the appearance of 15 lines of upland rice grown in Ultisol soil and choose the best lines based on index selection. The research was conducted on August 2015 until January 2016 at New Field Experiment Garden of Agriculture Faculty of Bengkulu University of Kandang Limun Sub-District, Muara Bangkahulu Sub-district, Bengkulu City. The experimental design was Randomized Block Design (RAKL) with 3 replications. As the treatment is 15 lines of rice are G1, G2, G3, G5, G8, G13, G16, G24, G30, G32, G35, G40, G44, G45, and G50. Of the 15 gogo rice strains tested showed that the generative and vegetative variables did not significantly affect the number of leaves, the number of productive tillers, the number of pithy grains per panicle, the weight of the grain per panicle, the weight of 100 grains, and the weight of grain per hill.keywords: upland rice, Ultisol, yield
Penapisan Tiga Puluh Tujuh Genotipe Tomat dan Seleksi Primer RAPD untuk Toleransi terhadap Layu Bakteri (Ralstonia solanacearum) Adeputri, Eriana; Rustikawati, Rustikawati; Herison, Catur
Akta Agrosia Vol 19 No 1 (2016)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Bacterial wilt (Ralstonia solanacearum) is the most important tomato diseases which can reduce tomato yield up to 100%. One most prospective control measure is the development of high yielding varieties tolerance to bacterial wilt. Donor parent carrying bacterial wilt controlling gene(s) is required to develop such varieties. The objectives of this study were to screen thirty-seven tomato genotypes for tolerance to R. solanacearum and to obtain RAPD markers of resistance to bacterial wilt. The experiment was arranged without the experimental design. Each tomato genotypes consisting of 5 control uninoculated plants and 10 plants were inoculated with R. solanacearum with a concentration of 106 cfu / ml inoculum of 10 mL per plant. Scoring was done on the severity of disease symptom; and the plants were grouped according to scoring class. Molecular analysis was done by using Bulk Segregant Analysis (BSA). The results showed there were six genotypes considerd very tolerant i.e. genotype Pearl, Opal, Cung, Syu and Kudamati I; tolerant genotypes consisting of five genotypes; medium tolerant groups consisting of eight genotypes; medium sensitives groups consisting of 14 genotypes; and sensitive class consisting of four genotypes, namely Aceh Local Tomatoes I, Tanah Datar Local Tomato, Tomato Meranti I and Tomato Meranti II. Of the 20 primers used only 8 primers showed visible DNA bands, they were E1, E7, E10, H2, H5, H13, H16 and H19. However there no polymorphics bands observed for bacterial wilt tolerance.
Combining ability of six s7 generation of corn hybrid parents from half dialel crossesof Maize in a Half Diallel Crosses Dwitama, Niko; Rustikawati, Rustikawati; Suryati, Dotti; Herison, Catur
Akta Agrosia Vol 21 No 1 (2018)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Combining ability is the genotype's ability to pass on the desired character to the offspring.  Combining ability information is needed to determine the crossed pairs in the formation of hybrid varieties.  The purpose of this study was to obtain information on the combining ability value of six S7 generation of inbreed maize.  The experiment was conducted in September 2015 until January 2016, at Medan Baru Experimental Station of Faculty of Agriculture, at Kandang Limun village, Muara Bangkahulu Sub-district, Bengkulu City.  The experiment was prepared in a Randomized Block Design (RCBD) with 15 corn hybrid treatments including: G1XG3, G1XG6, G3XG6, G1XG7, G3XG7, G6XG7, G1XG8, G3XG8, G6XG8, G7XG8, G1XG9, G3XG9, G6XG9, G7XG9 and G8xG9.  Each experimental unit was repeated three times obtaining 45 experimental plots.  Estimation of general combining ability (GCA) value  and specific combining ability (SCA) of each inbreed strain were conducted with Griffing model IV method.  The results showed that genotype G1 and G3 had positive GCA values for all characters except plant height.  The G7XG1 cross combination showed a positive SCA on all characters even higher in plant height and stem diameter. The G8XG6 cross combination showed high SCA values for leaf number characters, weight of husk-less cob, number of seeds per seed-line, weight of grain per ear, and grain yield per plot.Keywords: Hybrid, corn, GCA and SCA
Morphological Characterization of 10 Hot Pepper Genotipes in Low Altitude Location Herison, Catur; Surmaini, Ervi; Rustikawati, Rustikawati; Yulian, Yulian
Akta Agrosia Vol 21 No 2 (2018)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Development of high yielding and superior cultivars requires information on characteristic of germplasm will be used. Ten curly-type hot pepper genotypes was grown in a completely randomized design with three replication in a low altitude land to determine their morphological characters and to evaluate genetic relationship among them based on their morphological similarities. The study was conducted in September 2015 until January 2016 in Medan Baru experimental station of Faculty of Agriculture, 15 m above sea level.  Observation was conducted on both quantitative and qualitative characters.  The results showed that there were not significantly diferent (?=5%) for plant habitus, leaf shape, leaf tip, leaf edge, leaf shape and seed shape.  Whereas for quantitative variables of stem diameter, number of fruits per plants and fruit length were significantly different, while for the other variables were not.  Cluster analysis with phylogenetic trees in 58% similarity coefficient resulted that the genotypes grouped in to six groups.  Group I consisted of genotype LPK and Ferosa, group II Mario, group III was genotype Laris, group IV consisted of genotype Kopay and Romario, group V KH and L Curup, and group VI included genotype Bogota and Sempurna. The Kopay and Romario genotypes have the closest relationship level of 73%, while Local Payakumbuh and Lokal Curup have the farrest relationship with the coefficient of 35%. From the results of the study it can be concluded that Local Payakumbuh and Local genotypes Curup can be used as parents with the highest probability to have high transgresive segregation or highest hybrid vigor. Keywords: morphological characterization, genetic relationship, curly hot pepper
Effect of Several Types and Concentrations of Complex Organic Compounds on Growth of Pineapple In Vitro Sianipar, Mangara W; Rustikawati, Rustikawati; Bertham, Yudhy H; Herison, Catur; Mukhtasar, Mukhtasar
Akta Agrosia Vol 22 No 1 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Pineapple plants are usually propagated vegetatively by utilizing the base of the fruit (ratoone), stem buds (sucker), fruit buds (slip) and crown (crown). Propagation of plants by utilizing parts of the plant is less effective in meeting the needs because the planting material produced is small and requires a long time. An alternative to the propagation of pineapple to increase the amount of planting material is by tissue culture techniques. This study aims to obtain the best types of complex organic compounds and the right concentration for the growth of pineapple shoots in vitro. The research was conducted in June 2017 until October 2017 at the Laboratory of Agronomy at the Faculty of Agriculture, University of Bengkulu. Complete randomized design with 3 replications was used to allocate 9 treatments of complex organic compounds. The basic media used are the composition of Murashige and Skoog (MS). Treatment consists of 1) Coconut water 100 ml / l, 2) Coconut water 150 ml / l, 3) Coconut water 200 ml / l, 4) Banana porridge 100 gl / l, 5) Banana pulp 150 g / l, 6) Banana slurry 200 g / l, 7) Bean sprout extract 100 g / l, 8) Bean sprout extract 150 g / l, 9) Bean sprout extract 200 g / l. The results showed that at 16 weeks after MS media planting the addition of 100 g / l banana pulp was able to produce the highest plant height with an average of 3.45 cm. MS media with the addition of 200 ml coconut water resulted in the highest shoot height of 1.5 cm and followed by MS with the addition of 100 g / l media of banana pulp with an average of 0.9 cm. MS media with the addition of 100 ml / l of coconut water is suitable for the growth of pineapple roots with the average value of the number of roots and the highest length of roots. MS media with the addition of bean sprout extract with various concentrations is not able to stimulate shoot growth and has a growth response that tends to be slow. Keywords: Pineapple, In Vitro, Complex Organic Compounds.
Population Characteristic and Genetic Relationship on 25 Bengkulu Heirloom Rice Based on Morpgological Traits Rustikawati, Rustikawati; Simarmata, Marulak; Pujiwati, Hesti; Herison, Catur
Akta Agrosia Vol 22 No 2 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Bengkulu Province has many local rice germplasms that have adapted to specific areas. The germplasm has certain character advantages so that it is maintained by farmers. The characteristics of each germplasm need to be identified in order to improve the properties of the existing germplasm. This study aimed to determine the performance, to estimate the value of genetic parameters, and to determine the genetic relationship of local rice genotypes collected from four districts in Bengkulu Province. The results showed that the ‘Kuning Sulaowangi’ genotype was the earliest in maturity had the highest number of tillers amongst genotypes. Genotypes which have significantly the highest average component yields were ‘Kunig Air Dingin’ and ‘Impera’.  Traits that have a high heritability estimated value were weight of 100 grains (91.046%) and grain weight per panicle (80.252%). The weight of 100 grains also has the highest genetic variability value. Thus increasing local rice production is possible. Based on the results of the analysis with the similarity coefficient of 55%, the local rice genotypes could be grouped into two groups.  Group I consisted of 9 genotypes namely ‘Kuning Pendek’, ‘Humbur’, ‘Cantik’, ‘Pandak Kelabu’, ‘Cisadane Putih’, ‘Kuning Sulaowangi’, ‘China Abang’, ‘China Putih’ and ‘Impera’. Other genotypes were in Group II.  Trait improvement can be done through hybridization between individuals of different groups. To develope early in maturity and high-yielding varieties can be done through hybridization of the ‘Kunig Sulaowangi’ and ‘Kunig Air Dingin’
Manually Feed NPK Fertigation Improves Growth and Yield of Chili Pepper Hybrids in Ultisol Herison, Catur; Rustikawati, Rustikawati; Hasanudin, Hasanudin; Suharjo, Usman K.J.; Handajaningsih, Merakati; Nursalim, Nursalim
Akta Agrosia Vol 23 No 1 (2020)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Extending the planting area to a less-fertile soil, such as Ultisols, become inevitable choice to increase the national chili pepper production in Indonesia as this types of soil is the largest part of dry land of Indonesia, about 29.7% or around 51 million ha.  However, efforts to increase its productivity by providing sufficient fertilizers application is most frequently inefficient because in the conventional fertilizer applications, broadcasted fertilizer on the soil bed, are not readily available for the plant as their solubility is limited under the plastic mulch and still in the form of granular until the end of planting season.  Fertigation, therefore, maybe the answer for this problem.  The objective of this study was to determine the best NPK fertigation method on four chili pepper hybrids.  The experiment was conducted in a randomized complete block design with three replication, and the treatments were arranged in a split plot design.  As the main plots were NPK fertigation intensity, i.e. 100%, 75%, 50%, 25% and 0% fertigation with the remnant fertilizers were circularly broadcasted surrounding the plant.  As the subplots were chili pepper hybrids, i.e. ‘Maxima’, H39, H14, and ‘UNIB CHR17’.  The results showed that there was no interaction effect of fertigation intensity indicated that all hybrid genotypes showed similar pattern of responses.  The H39 hybrid had better vegetative growth compared to other hybrids in term of plant height, dichotomous height, stem diameter, number of branches, plant fresh-weight.  Whereas the hybrid cultivar ‘Maxima’ showed the highest yield followed by H14 and cultivar ‘UNIB CHR17’.  The 100% fertigation was the best method of NPK fertilizer application for chili pepper in Ultisols.keywowrd: fertigation, chili pepper, fertilizer application, acidic soil
Co-Authors A.A. Ketut Agung Cahyawan W A.W. Sandhi S.G. Adeputri, Eriana Alnopri Alnopri, Alnopri Atra Romeida Bertham, Yudhy H Catur Herison Dewi Liesnoor Setyowati Dwi Wahyuni Ganefianti, Dwi Wahyuni Dwitama, Niko EDHI TURMUDI Eko Suprijono Ela Hasri Windari, Ela Hasri Windari Fahrurrozi Fahrurrozi Fernandez, Regi Gustian, Meko Hasanudin Hasanudin Herison, Catur HESTI PUJIwATI Hindarto, Kanang Setyo Indres, Indres Inoriyah, Entang Luwih, Bagus Edi Marulak Simarmata Merakati Handajaningsih Merakati Handayaningsih Mimi Sutrawati Muhammad Chozin, Muhammad Mukhtasar, Mukhtasar Nurjanah, Uswatun Nursalim Nursalim Reny Herawati S. H. Sutjahjo Saputra, Pringky Okta Sianipar, Mangara W Sri Wulandari Sri Wulandari Wulandari, Sri Wulandari Suharjo, Usman K.J. Sukisno Sukisno, Sukisno Surjono H. Sutjahjo Surmaini, Ervi Suryati, Dotti Windari, Ela Hasri Wuri Prameswari Yulian, Yulian