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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Jurnal Pengabdian Magister Pendidikan IPA Jurnal Ilmiah Profesi Pendidikan Journal of Classroom Action Research Bioscientist : Jurnal Ilmiah Biologi
PRAPTI SEDIJANI, PRAPTI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Physics Social Sciences Other

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Effect of Silver Black Plastic Mulch and Organic Fertilizer Dosage on Plant Chlorophyll Growth and Content Cayenne Pepper (Capsicum annum L.) Gifridus, Valeria; Sedijani, Prapti; Raksun, Ahmad
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.4908

Abstract

Cayenne pepper is one of the leading horticultural products in the agricultural sector in Indonesia. In order for plants to grow optimally and produce well, it is necessary to apply silver black plastic mulch and a dose of organic fertilizer. This study aims to determine the growth and chlorophyll content of cayenne pepper plants using black silver plastic mulch and doses of organic fertilizer. The study used a randomized block design with 8 treatment combinations and 3 repetitions. The arrangement of treatments was as follows: M0P0: without mulch + 0 kg/m2, M0P1: without mulch+ 1 kg/m2, M0P2: without mulch+ 1.5 kg/m2, M0P3: without mulch+ 2 kg/m2, M1P0: MPHP + 0 kg /m2, M1P1: MPHP + 1 kg/m2, M1P2: MPHP + 1.5 kg/m2, M1P3: MPHP + 2 kg/m2. The results on plant height and leaf number observations on days 28 and 40 showed that the silver black plastic mulch factor make a real impact. Organic fertilizer significantly affected leaf length on day 40, while the interaction of silver black plastic mulch and dose of organic fertilizer affected leaf length and leaf area on day 40. The best combination in this study was black silver plastic mulch and a dose of 1.5 kg organic fertilizer/m2.
Molecular Characterization of Neem Rhizosphere Bacteria as A Nitrogen Fixer Isolated from The Dry Land of East Lombok Umami, Rizal; Zulkifli, Lalu; Bahri, Syamsul; Mahrus; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6122

Abstract

Bacteria have an important role in providing essential nutrients for plants in dry land through various beneficial working mechanisms. One of the main mechanisms is the involvement of bacteria in nutrient cycles, such as the nitrogen cycle. The aim of this research was to determine the type and ability of bacteria in the rhizosphere of neem (Azadirachta indica) to fix nitrogen. The method used in this research is an exploratory descriptive type. The results of the isolation carried out from the rhizosphere of neem (Azadirachta indica) taken from the East Lombok area of Jerowaru sub-district, 5 samples were obtained with the code LO, where 2 isolates had the ability to fix nitrogen, namely LO3 and LO4. This is proven by using NFB media where there is a change in the color of the media from green to blue, and quantitatively using a spectrophotometer with results of 1,112 ppm and 2,156 ppm, respectively. The results of the molecular identification of the potential bacteria, namely LO4, using the BLAST method at the GenBank data base (NCBI), show that LO4 is the closest relationship to the species Lysinibacillus fusiformis strain SMC303. The results above show that rhizobacteria isolated from neem have the potential to be developed as a biofertilizer that can be applied to future cultivated plants.
Isolation and Identification of Phosphate Solubilizing Bacteria from The Rhizosphere of Dry Land Lamtoro Plants (Leucaena leucocephala) in North and South Lombok Regions Hartanto, Priyo; Zulkifli, Lalu; Karnan; Sedijani, Prapti; Mahrus
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6127

Abstract

Phosphate solubilizing rhizobacteria are microorganisms that live in the rhizosphere of plants and have the ability to increase the availability of phosphate in the soil, thereby reducing dependence on chemical fertilizers which are harmful to the environment. This research aims to isolate rhizosphere bacteria from Lamtoro plants and test their ability to dissolve phosphate. Screening the ability of these bacterial isolates was carried out using Pikovskaya media and quantitatively using a spectrophotometer. The test results showed that there were 3 isolates, namely KLU13, A2 and KLS1, which had IKF values in the medium category. Meanwhile, the other 4 isolates had abilities in the low category (isolates A1, A31BK, A3BB and A3). The results of quantitative tests with a spectrophotometer showed that there was an increase in dissolved phosphate from day 2 to day 6. Isolates KLU13, A3 and KLS1 dissolved the highest phosphate on day 6 with values of 14.65 ppm, 14.81 ppm and 10.32 ppm respectively. It can be concluded that the isolate obtained from the Lamtoro rhizosphere which grows in the dry areas of Lombok Island has the potential to be developed as a biofertilizer in the future.
Penambahan Kulit Kacang Tanah pada Medium Jamur Menambah Berat Kering Jamur Tiram Putih Sedijani, Prapti; Rahmi, Maskatur; Japa , Lalu; Eniarti, Miko
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.6182

Abstract

Medium composition affects the productivity of oyster mushroom. Nutritious ingredient may increase the mushroom productivity. Peanut shell as a leguminous plant is rich in protein content than zea mays bran a common supplement for the medium. This research ach aims to see the influence of peanut shell supplementation at different concentration on productivity of oyster mushroom in comparison to zea mays bran. The concentration used were 0; 3 and 6% of peanut shell or zea mays bran. The parameter observed were the time of primordial pin head appearance; the pin head number, fresh weigh and dry weight of oyster mushroom. The data obtained were analyzed using ONE WAY ANOVA followed by Least Significant Different Test for the significance. The result showed that medium with Zea mays did not show a significant different from control in all parameters used. The peanut shell increased the dray weight of oyster mushroom, the dray weight increase was higher at supplementation of 3% peanut shell than that of 6%. The fresh weight and number fruiting body were not significantly different among all the treatments, however, the average of the fresh weigh and the number of pin-head, were higher found on medium supplemented with peanut shell that others. Three percentage of peanut shell was better than that of 6%.
Test The Effect of The Inhibitory Power and Minimum Killing Power of Clove Flower Oil (Syzigum aromaticum) with Water as a Solvent Against Staphylococcus aureus Putra, Hendra Susana; Mahrus; Sedijani, Prapti; Al Idrus, Agil; Zulkifli, Lalu
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6188

Abstract

Excessive use of antibiotics in treating infectious diseases has caused some bacteria to become resistant. Therefore, it is hoped that the use of natural ingredients such as cloves can be an alternative solution in reducing excessive use of antibiotics. This study aims to test the inhibitory effect and minimum killing power of clove flower oil (Aromatic syzygum) with water as a solvent against Staphylococcus aureus. In determining the inhibition zone, the diffusion disk method was used. Meanwhile, determining the Minimum Kill Ability (MKA) uses the liquid tube dilution method. The results showed that an increase in the concentration of clove flower extract was followed by an increase in the inhibition zone in the media with the isolate Staphylococcus aureus. Meanwhile, in measuring the Minimum Kill Ability, the results showed that the MKA value of clove leaf extract against bacteria Staphylococcus aureus smaller than 5%. This shows that only a 5% concentration of clove leaf extract is enough to inhibit growth Staphylococcus aureus. These findings indicate the potential of clove leaf extract as an antimicrobial agent for bacteria Staphylococcus aureus. So it is hoped that natural ingredients will be able to reduce excessive use of antibiotics.
Antagonistic Test of Bacteria Producing Siderophore and Protease Enzymes from The Rhizosfer of Peanut Plants on The Growth of Pathogenic Fungus Colletotrichum gloeosporioides Nurikhsanti, Mikiyal; Zulkifli, Lalu; Rasmi, Dewa Ayu Citra; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6459

Abstract

This study aims to determine the inhibitory effect of bacterial isolates producing siderophores and protease enzymes on the growth of the pathogenic fungus Colletotrichum gloeosporioides. The initial stage of research begins with the isolation of pathogenic bacteria and fungi, and is followed by testing the production of siderophores and protease enzymes. Bacteria were isolated from the rhizosphere of peanut plants, while pathogenic fungi were isolated from large chili fruit infected with anthracnose disease taken from Jelantik Village, Central Lombok Regency. Characterization of isolates for siderophore production used the Arnow method, while the protease enzyme production test used SKIM Milk Agar media. Next, the inhibition test of bacterial isolates against pathogenic fungi was carried out using the dual culture method. Characterization of potential isolates was carried out by observing bacterial colony morphology, gram staining and biochemical tests. The results of the siderophore production test showed that there was one isolate capable of producing siderophores with the isolate code RKT2. Meanwhile, the protease enzyme production test showed that all bacterial isolates produced protease enzymes, where isolate RKT9 had the highest Proteolytic Index, namely 1.57. The two isolates showed different inhibitory test results, namely isolate RKT2 had high inhibition, while RKT9 had low inhibition. The results of the research showed that a bacterial isolate (RKT2) from the rhizosphere of peanut plants was able to inhibit the growth of the pathogenic fungus Colletotrichum gloeosporioides in the high category.
The Effect of Green Tea (Camellia Sinensis L) Steeping on Blood Glucose Levels of Mice Ayuningtias, Wiranting; Bahri, Syamsul; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6648

Abstract

Diabetes mellitus (DM) is a chronic metabolic disorder caused by high blood sugar levels due to lack of insulin function. Insulin is a hormone that helps blood sugar enter. Insulin injections are the main therapy for treating diabetes mellitus, they are relatively expensive and long-term use can cause side effects such as hypoglycemia. Other alternatives need to be found for the treatment of Diabetes Mellitus (DM), one of which is green tea. The aim of this research was to determine the effect of steeping green tea on the blood glucose levels of mice (24 males) which were divided into 4 groups. Mice were selected using the Simple Random Sampling technique representing 4 dose groups, namely: control group, brewed green tea at a dose of 30mg/0.5cc/day, brewed green tea at a dose of 35mg/0.5cc/day and green tea. brew at a dose of 40mg/0.5cc/day. The treatment was given orally for 6 repetitions. For 15 consecutive days. Data analysis used the One ANOVA test with a significance level of 5% (p<0.05) followed by the LSD test with a confidence level of 95%. The results of the study showed that there was a significant effect on reducing glucose levels in mice. Mice given a dose of 30, 40 and 45% brewed green tea experienced a decrease in glucose levels of 43, 32 and 40.27% respectively compared to controls.
Preliminary Test of the Sensitivity of Colletotrichum spp. to Binahong Ethanol Extract (Anredera cordifolia) Aminatuzzifah, Putri; Bahri, Syamsul; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7840

Abstract

The agricultural sector in Indonesia, especially the horticultural subsector, plays an important role in the economy and food security. One of the leading commodities in horticulture is the chilli plant (Capsicum annuum), which is very susceptible to anthracnose disease caused by the fungus Colletotrichum spp. This study aims to test the inhibitory activity of ethanol extract from binahong (Anredera cordifolia) leaves against the growth of Colletotrichum spp. fungus as an alternative environmentally friendly disease control. The research was conducted using a completely randomised design (CRD) with variations in extract concentrations of 0%, 0.5%, 1%, 1.5%, 2%, and 2.5%, each of which was tested three times. The results showed that 2.5% concentration of binahong leaf extract produced the highest inhibitory activity of 70%, while 0.5% concentration showed 56% inhibition. This inhibitory activity was categorised as strong. ANOVA analysis showed significant differences between treatments (p < 0.05). These findings demonstrate the potential of binahong leaf extract as a biofungicide in the control of anthracnose disease in chilli plants, which may contribute to more sustainable and environmentally friendly agricultural practices.
Effect of NPK Fertilizer and Vermicompost on The Vegetative Growth of Green Eggplant (Solanum melongena L.) Putra, Masaradi; Raksun, Ahmad; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.7861

Abstract

Plant growth and development require nutrients, which can be provided through fertilization in the form of both inorganic and organic fertilizers. This study aims to determine the effect of NPK fertilizer, vermicompost, and the combination between the two on the vegetative growth of green eggplant. In this study, a two-factor Complete Random Design was used consisting of NPK fertilizer and vermicompost treatment with each using 5 treatment levels and carried out with 4 replicates. The parameters measured were stem height, number of leaves, leaf area, plant dry weight and plant wet weight. The data obtained was analyzed by the Anova two-way and continued with the DMRT. The results showed that the application of NPK fertilizer had a significant effect on all observed growth parameters. The application of vermicompost had a significant effect on all observed growth parameters. The application of a combination of NPK fertilizer and vermicompost had a significant effect on several growth parameters, including plant height, leaf area, dry weight  and wet weight of the plant, but  did not have a significant on the number of leaves.
Antagonistic Test of Endophytic Bacteria of Chili Pepper Roots Producing Siderophores and Hydrolase Enzymes against Plant Pathogenic Bacteria Ralstonia Solanacearum Hidayati, Siti Nur Isnaini; Zulkifli, Lalu; Sedijani, Prapti; Rasmi, Dewa Ayu Citra
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7966

Abstract

This study aims to test the ability of endophytic bacterial isolates of cayenne pepper roots (Capsicum frutescens) to produce siderophore compounds and protease enzymes and to test the antagonistic activity of endophytic bacterial isolates in controlling the growth of plant pathogenic bacteria (Ralstonia solanacearum). The stages in this study are isolation of endophytic bacteria from cayenne pepper roots, siderophore production test, protease enzyme activity test, and biochemical and physiological characterization of potential endophytic bacteria, antagonistic test of endophytic bacteria against the growth of Ralstonia solanacearum bacteria. Endophytic bacteria were isolated from cayenne pepper plant roots taken from Selong, East Lombok. Characterization of siderophore-producing isolates used the Arnow test method. The activity of bacterial protease enzymes was tested qualitatively on Skim Milk Agar solid media. The antagonistic activity test of endophytic bacteria against pathogenic bacteria was carried out using the Kirby-Bauer diffusion method. Characterization of endophytic bacteria was carried out by observing colony morphology, Gram staining, and biochemical tests. There were 3 isolates that were able to produce siderophores (catecholate type) with codes EC5, EC12, and EC15. Six isolates showed protease enzyme activity with codes EC3, EC4, EC5, EC7, EC11, and EC15, with the highest Proteolytic Index (2.02) shown by isolate EC4. There were 3 isolates that were able to inhibit the growth of Ralstonia solanacearum (EC4, EC5, and EC15), with the highest inhibitory power shown by isolate EC15 (3.67 mm). The results of the study indicate that endophytic bacteria from cayenne pepper plant roots have the potential as biopesticides in the future.
Co-Authors A. Wahab Jufri Agil Al Idrus Ahmad Raksun, Ahmad Aminatuzzifah, Putri Apriani, Yaumil Arifin, Ahmad Aris Ayuningtias, Wiranting Desi, Petronela Eniarti, Miko Gifridus, Valeria Gito Hadiprayitno Hidayati, Siti Nur Isnaini I Gde Mertha I WAYAN SUANA Ida Royani Ilhamdi, Mohammad Liwa Jamaluddin JANNATIN ARDHUHA, JANNATIN Japa , Lalu Karnan Karnan Khairuddin Khairuddin - Kusmiyati Kusmiyati Kusmiyati Lalu Japa, Lalu LALU RUDYAT TELLY SAVALAS LALU ZULKIFLI Larasati, Sri Aprilia Nur Lestari, Chomsa Asih M. Yamin Mahrus Muhidayani, RE Septi Muhidayani, Respi Eka Muhlis Nafi'ah, Zidna Ilma Nathnia, Elga Amelinda Ni Luh Putu Pebri Artayani . Nurikhsanti, Mikiyal Priyo Hartanto Putra, Hendra Susana Putra, Masaradi Rahmi, Maskatur Rasmi, Dewa Ayu Citra Risnawati Sabila, Sabila Nur Amalina Sabrina, Yolanda Sania, Neza Okta Saprizal Hadisaputra Saputri, Andini Sari, Neti Kumala Sativa, Denianto Yoga Sukarso, Aa SUYANTRI, ENI Syamsul Bahri UMAMI, RIZAL Utami, Ni Wayan Anggun Diah Wahidah, Etty Nurul Yayuk Andayani Zulkifili, Lalu