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The Effect of Liquid Organic Fertilizer (LOF) from Rotten Fruit on the Growth of Chili Pepper Plants (Capsicum frutescens L.) Nafi'ah, Zidna Ilma; Sedijani, Prapti; Japa, Lalu
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10408

Abstract

Fruit trash is a waste product that is typically dumped in the open without any additional handling, which can lead to illness, contamination of the air and water, and other issues. This study aims to analyze the effect and determine the right dose of using liquid organic fertilizer from rotten fruit on the growth of plant of cayenne pepper (Capsicum frutescens L.). This study was an experimental with a randomized design (CRD). There were 5 treatments, namely P0 (control/using 1000 mL water), P3 (300 mL LOF + 700 mL water or 30% concentration), P6 (600 mL LOF + 400 mL water or 60% concentration), P9: 900 mL LOF + 100 mL water or 90% concentration, and P12 (1200 mL liquid organic fertilizer or 100% concentration). Vegetative development (stem height, leaf area, and number of leaves) and generative growth (fruit fresh weight) were among the measurement criteria. The statistical test (ANOVA) was used to analyze the data at a significance level of 0.05. The outcome shown that the growth of cayenne pepper was considerably impacted by the usage of rotting fruit LOF. Following additional testing using the least significant difference test (LSD), the study's findings indicated that 60% (P6) of rotten fruit LOF was the ideal dosage for cayenne pepper's vegetative and generative growth, with an average plant stem height of 35.10 cm, leaf area of 53.90 cm2, number of leaves of 37 strands, and fresh fruit weight of 55.35 grams.
Pengembangan LKPD Praktikum Materi Karakteristik Makhluk Hidup Terhadap Hasil Belajar Siswa Kelas VII SMP Negeri 3 Gunungsari Kusuma, Nila; Handayani, Baiq Sri; Sedijani, Prapti
Journal of Authentic Research Vol. 4 No. 2 (2025): December
Publisher : LITPAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/6a3g8g06

Abstract

Kegiatan praktikum di SMPN 3 Gunungsari masih belum optimal karena digunakan guru masih bersifat umum, hanya berupa ringkasan materi, dan belum sepenuhnya melibatkan siswa dalam kegiatan praktikum. Kondisi ini menyebabkan peserta didik kesulitan memahami langkah percobaan dan konsep yang dipelajari, sehingga aktivitas dan hasil belajar belum maksimal. Penelitian ini bertujuan untuk mengetahui pengembangan LKPD praktikum pada materi karakteristik makhluk hidup yang valid, praktis dan efektif untuk siswa kelas VII  SMP. Penelitian ini menggunakan metode pengembangan dan penelitian (Research and Development). Desain penelitian ini menggunakan ADDIE yang dimodfikasi pada tiga tahap (analisis, desain, dan pengembangan).Kebaruan penelitian ini terletak pada penyusunan LKPD dengan langkah-langkah ilmiah Kurikulum Merdeka, penggunaan aktivitas praktikum berbahan sederhana, serta tampilan visual yang komunikatif. Hasil validasi menunjukkan LKPD sangat valid (nilai ahli media 88,67% dan ahli materi 85,14%). Uji kepraktisan kepada 30 siswa menunjukkan nilai 93,73% (sangat praktis). Efektifitas diperoleh dari nilai N-Gain sebesar 0,74 (kategori tinggi). Dengan demikian, LKPD praktikum ini layak digunakan dan terbukti dapat meningkatkan hasil belajar siswa.
Validitas dan Kepraktisan Modul Pembelajaran IPA Berbasis Proyek Biopeneurship untuk Meningkatkan Keterampilan Abad-21 pada Siswa SMP Buka, Listiawati H.; Jufri, A Wahab; Sedijani, Prapti
Journal of Classroom Action Research Vol. 7 No. 4 (2025): November
Publisher : Program Studi Magister Pendidikan IPA, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jcar.v7i4.14424

Abstract

Tuntutan penguasaan keterampilan abad ke-21 menempatkan pembelajaran IPA tidak hanya berorientasi pada penguasaan konsep, tetapi juga pada pengembangan kemampuan berpikir kritis, keterampilan proses sains, dan minat wirausaha siswa melalui pembelajaran yang berpusat pada siswa dan kontekstual. Namun, tuntutan tersebut belum sepenuhnya tercermin dalam praktik pembelajaran IPA di sekolah yang masih didominasi oleh penggunaan buku teks terbitan Kementerian Pendidikan. Penelitian ini bertujuan untuk mengembangkan modul pembelajaran IPA berbasis proyek biopreneurship yang valid dan praktis untuk meningkatkan kemampuan berpikir kritis, keterampilan proses sains, dan minat wirausaha siswa SMP. Penelitian ini merupakan penelitian dan pengembangan menggunakan model Four-D. Hasil validasi oleh tiga pakar ahli menunjukkan bahwa modul dan instrumen evaluasi memperoleh nilai rata-rata indeks Aiken’s V lebih dari 80%. Selain itu, respon guru dan siswa menunjukkan bahwa modul dinilai praktis dan mudah digunakan dalam pembelajaran. Dengan demikian, modul pembelajaran IPA berbasis proyek biopreneurship dinyatakan layak dan praktis untuk mendukung pengembangan keterampilan abad ke-21 siswa.
Isolation and Identification of Endophytic Bacteria Producing IAA and Phosphate Solubilizers from Mangrove Roots in the Bagek Kembar Sekotong Area and In Vitro Testing on the Germination of Vigna radiata L. Havizah, Ilma Nur; Zulkifli, Lalu; Rasmi, Dewa Ayu Citra; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.10069

Abstract

Mangroves are a potential source of plant growth-promoting bacteria (PGPB) due to their unique habitat. Exploring potential endophytes as biofertilizers to reduce dependence on chemical fertilizers. This study aims to determine the effect of IAA-producing endophytic bacteria and phosphate solubilizers from mangrove roots on the germination of mung beans (Vigna radiata L.). The bacteria were isolated from mangrove roots in the Bagek Kembar Mangrove Ecosystem Area, Sekotong, West Lombok. The study began with the isolation of endophytic bacteria, followed by characterization, including morphological identification, cell morphology (via Gram staining), and physiological properties (biochemical testing). The potential of endophytic bacteria to produce IAA was tested qualitatively (using colorimetry) and quantitatively (using a spectrophotometer at a wavelength of 530 nm). Additionally, the ability of endophytic bacteria to solubilize phosphate was tested both qualitatively (using a screening method) and quantitatively (using a spectrophotometer at a wavelength of 880 nm). To test the effect of endophytic bacteria on soybean germination, green soybean seeds were grown on modified Murphy medium for 5 days, with the observed parameters being plant height, root length, fresh weight, and dry weight. The results of the green soybean germination observations were analyzed using One-Way ANOVA. Based on the research results, 11 endophytic bacterial isolates were found to be capable of producing IAA in the range of 16.88 to 30.28 ppm. Meanwhile, in terms of phosphate solubility, based on the screening results, 6 out of 11 isolates were able to solubilize phosphate with an average dissolved phosphate concentration of 17.63 ppm on the 4th day of incubation. The results of the analysis of the effect of IAA-producing bacteria and phosphate solubilizers on the germination of green beans showed a significant effect on plant height, root length, fresh weight, and dry weight of green bean plants.
Isolation and Identification of IAA-Producing and Phosphate-Solubilizing Rhizobacteria from Sesuvium portulacastrum and In Vitro Growth-Promotion Effect on Vigna radiata L. Yuliana, Baiq Fadilla; Zulkifli, Lalu; Rasmi, Dewa Ayu Citra; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10070

Abstract

Sesuvium portulacastrum is a coastal plant resistant to high salt content and drought. Exploration of rhizosphere bacteria is necessary to obtain potential isolates that can be developed as biofertilizers in the future. This study aims to isolate and identify rhizosphere bacteria from Sesuvium portulacastrum that are able to produce Indole Acetic Acid (IAA) and dissolve phosphate, and to test their effects in vitro on the germination of Vigna radiata L. Rhizosphere bacteria from the Bagek Kembar Mangrove Ecosystem Area, Sekotong, West Lombok Regency. A total of 14 bacterial isolates were successfully obtained and characterized based on morphological, physiological, and biochemical properties. All isolates produced IAA qualitatively and quantitatively (19.85–33.71 ppm) with isolate KT1 having the highest production (33.71 ppm). Qualitative phosphate solubilization test showed that 12 isolates were able to form a clear zone, while quantitatively it ranged from 7.86 to 16.18 ppm with isolate K8 having the highest value (16.18 ppm). Selected isolates KT1 (the highest IAA producer), K8 (the highest phosphate solubilizer), and K5 (both with high abilities) were tested for their effect on mung bean germination. Inoculation had a significant effect on plant height, root length, and dry weight (p<0.05). These results indicate that S. portulacastrum rhizosphere bacteria have the potential to be an environmentally friendly biofertilizer for plants in high-salinity soils.
Analisys of Lead content in Tilapia (Oreochromis niloticus) from Taliwang Lake Khairuddin, Khairuddin; Yamin, M.; Kusmiyati, Kusmiyati; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.10772

Abstract

Research on heavy metals is important to be done to protect consumers from dangerous impact especially on human beings. The purpose of this study was to determine the Lead (Pb) content in Tilapia (Oreochromis niloticus) taken from Rawa Taliwang Lake. Specifically, the benefit of this study is to protect consumers who consume Tilapia from Lead heavy metal contaminants. Data collection for the tilapia species in this study was carried out in water bodies within the Taliwang Lake area. There were 2 data collection stations, namely on the east and west sides of the lake. Gill nets were used to catch tilapia. Fish samples were caught as many as 4 fish for each station. Next, each fish sample was put into a plastic bag and then stored in a sample box. The fish samples were then analyzed at the West Nusa Tenggara Health, Testing and Calibration Laboratory Center. The data analysis method was carried out by taking muscle tissue from the fish and then analyzing the Pb heavy metal content using an Atomic Absorption Spectrophotometer. The concentration of Lead in fish tissue was measured after adding concentrated Na2SO4 and CuSO4, which were heated at 350ºC for 2-3 hours until the solution was clear. The conclusion of this study is that the heavy metal content of Pb in Tilapia (Oreochromis niloticus) is <0.5 ppm.  
Measurement of Lead content in Channa striata by using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) Khairuddin, Khairuddin; Yamin, M.; Kusmiyati, Kusmiyati; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.11081

Abstract

Research on heavy metals Pb is important to be done to protect consumers from dangerous impact, especially on human beings. The purpose of this study was to determine the Lead (Pb) content in Snakehead fish (Channa striata) taken from Rawa Taliwang Lake. Data collection for the Snakehead fish (Channa striata) species in this study was carried out in water bodies of Taliwang Lake area. There were 2 data collection stations, namely on the east and west parts of the lake. Gill nets were used to catch fish. Fish samples were caught as many as 4 fish for each station. The fish samples were then analyzed at the West Nusa Tenggara Health, Testing, and Calibration Laboratory Center. The data analysis method was carried out by taking muscle tissue from the fish and then analyzing the Pb heavy metal content using an Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Pb found in Snakehead fish in this research is 0,44 ppm. The benefit of this study is to protect consumers who consume Snakehead fish from Lead heavy metal contaminants. The concentration of Lead in fish tissue was measured after adding concentrated 10 ml HNO3 and 2 ml H2O2 30%. The conclusion of this study is that the heavy metal content of Pb in Channa striata is 0.44 ppm.Reaseach on Pb heavy metal sould be continued in order to protect consumers from metal contamination.
Japanese Papaya (Cnidoscolus aconitifolius): An Integrated Biological System with Mechanism-Based Utilization Sedijani, Prapti; Mahrus, Mahrus; Rasmi, Dewa Ayu Citra; Kusmiyati, Kusmiyati; Khaerudin, Khaerudin
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.11234

Abstract

Cnidoscolus aconitifolius (Japanese papaya or chaya) is a tropical plant from the Euphorbiaceae family known for its high nutritional value and diverse biological activities. Several studies have reported antioxidant, antimicrobial, and other biological properties, suggesting the involvement of complex molecular systems. This article presents a conceptual literature review using a systems biology approach to understand Japanese papaya as an integrated biological system in which nutritional components, secondary metabolites, and enzymatic activities interact. By positioning hydrolytic enzyme systems as the central mechanism, this review discusses the potential utilization of Japanese papaya as a biological resource and as a basis for the development of functional derivative products, including biomolecular hydrolysates. Possible applications in mechanism-based bioconversion processes are also briefly addressed.
Study of the Difference in Biodegradation Rate of LAS (Linear Alkylbenzene Sulfonates) and MES (Methyl Ester Sulfonate) Surfactants by Pseudomonas putida and Bacillus megaterium Dzurrahmi, Baiq Dini Najia; Sedijani, Prapti; Muhlis, Muhlis
Jurnal Biologi Tropis Vol. 26 No. 1 (2026): Januari-Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i1.11387

Abstract

Linear Alkylbenzene Sulfonate (LAS) is the most widely used surfactant in industrial detergent formulations, generating waste that poses potential risks to environmental quality and human health. As an environmentally friendly alternative, palm oil–based Methyl Ester Sulfonate (MES) has renewable and biodegradable properties, stability in hard water and high salinity, and a faster degradation rate than LAS. However, direct comparative studies on the biodegradation rates of LAS and MES are still limited. Therefore, this study aimed to compare the biodegradation ability of Pseudomonas putida and Bacillus megaterium toward LAS and MES in vitro using the MBAS method. The experiment employed a Completely Randomized Design with a two-factor factorial arrangement and three replications, resulting in 12 experimental units. Biodegradation rates were determined by measuring surfactant concentrations at days 0, 2, 4, 6, and 8 using UV-Vis spectrophotometry and linear regression analysis. The results showed a decrease in surfactant concentration over time in all treatments. Statistical analysis indicated no significant difference between LAS and MES biodegradation rates, but significant differences between bacterial species and their interaction with surfactant type.
Water Quality Assessment of Bagek Kembar Mangrove Ecosystem Using Phytoplankton as A Bioindicators Setiawan, Yoszi; Japa, Lalu; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 26 No. 1 (2026): Januari-Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i1.11492

Abstract

Mangrove ecosystems are highly dependent on surrounding water quality, which can be effectively assessed using biological indicators such as phytoplankton communities. This study aimed to analyze the water quality of the Bagek Kembar Mangrove Essential Ecosystem, West Lombok, based on phytoplankton community. The research employed a descriptive exploratory design with purposive sampling at three mangrove ecosystem types: pond area, rehabilitated mangrove, and natural mangrove. Phytoplankton samples were collected using a plankton net and analyzed for abundance, species diversity, evenness, and dominance. Physical and chemical parameters including temperature, pH, and salinity were also  study fully identified 67 species of phytoplankton with a total abundance of 2,542.5 ind/L. Merismopedia elegans was a dominant species, particularly in the pond area. Diversity indices ranged from low to moderate, evenness valuew indicated relatively stable communities, and dominance indexs showed no species dominance. These results indicated that the waters of the Bagek Kembar mangrove ecosystem are generally in good condition with mild ecological pressure influenced by anthropogenic activities. This study provides baseline ecological data to support sustainable management and conservation strategies for mangrove essential ecosystems of Bagek kembar, West Lombok.