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All Journal Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Jurnal Pengabdian Magister Pendidikan IPA Jurnal Ilmiah Profesi Pendidikan Journal of Classroom Action Research Journal of Authentic Research Indonesian Journal of Education and Community Services
PRAPTI SEDIJANI, PRAPTI
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology Languange, Linguistic, Communication & Media Public Health Social Sciences Other

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Pengembangan LKPD Praktikum Materi Karakteristik Makhluk Hidup Terhadap Hasil Belajar Siswa Kelas VII SMP Negeri 3 Gunungsari Kusuma, Nila; Handayani, Baiq Sri; Sedijani, Prapti
Journal of Authentic Research Vol. 4 No. 2 (2025): December
Publisher : LITPAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/6a3g8g06

Abstract

Kegiatan praktikum di SMPN 3 Gunungsari masih belum optimal karena digunakan guru masih bersifat umum, hanya berupa ringkasan materi, dan belum sepenuhnya melibatkan siswa dalam kegiatan praktikum. Kondisi ini menyebabkan peserta didik kesulitan memahami langkah percobaan dan konsep yang dipelajari, sehingga aktivitas dan hasil belajar belum maksimal. Penelitian ini bertujuan untuk mengetahui pengembangan LKPD praktikum pada materi karakteristik makhluk hidup yang valid, praktis dan efektif untuk siswa kelas VII  SMP. Penelitian ini menggunakan metode pengembangan dan penelitian (Research and Development). Desain penelitian ini menggunakan ADDIE yang dimodfikasi pada tiga tahap (analisis, desain, dan pengembangan).Kebaruan penelitian ini terletak pada penyusunan LKPD dengan langkah-langkah ilmiah Kurikulum Merdeka, penggunaan aktivitas praktikum berbahan sederhana, serta tampilan visual yang komunikatif. Hasil validasi menunjukkan LKPD sangat valid (nilai ahli media 88,67% dan ahli materi 85,14%). Uji kepraktisan kepada 30 siswa menunjukkan nilai 93,73% (sangat praktis). Efektifitas diperoleh dari nilai N-Gain sebesar 0,74 (kategori tinggi). Dengan demikian, LKPD praktikum ini layak digunakan dan terbukti dapat meningkatkan hasil belajar siswa.
Isolation and Identification of Endophytic Bacteria Producing IAA and Phosphate Solubilizers from Mangrove Roots in the Bagek Kembar Sekotong Area and In Vitro Testing on the Germination of Vigna radiata L. Havizah, Ilma Nur; Zulkifli, Lalu; Rasmi, Dewa Ayu Citra; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.10069

Abstract

Mangroves are a potential source of plant growth-promoting bacteria (PGPB) due to their unique habitat. Exploring potential endophytes as biofertilizers to reduce dependence on chemical fertilizers. This study aims to determine the effect of IAA-producing endophytic bacteria and phosphate solubilizers from mangrove roots on the germination of mung beans (Vigna radiata L.). The bacteria were isolated from mangrove roots in the Bagek Kembar Mangrove Ecosystem Area, Sekotong, West Lombok. The study began with the isolation of endophytic bacteria, followed by characterization, including morphological identification, cell morphology (via Gram staining), and physiological properties (biochemical testing). The potential of endophytic bacteria to produce IAA was tested qualitatively (using colorimetry) and quantitatively (using a spectrophotometer at a wavelength of 530 nm). Additionally, the ability of endophytic bacteria to solubilize phosphate was tested both qualitatively (using a screening method) and quantitatively (using a spectrophotometer at a wavelength of 880 nm). To test the effect of endophytic bacteria on soybean germination, green soybean seeds were grown on modified Murphy medium for 5 days, with the observed parameters being plant height, root length, fresh weight, and dry weight. The results of the green soybean germination observations were analyzed using One-Way ANOVA. Based on the research results, 11 endophytic bacterial isolates were found to be capable of producing IAA in the range of 16.88 to 30.28 ppm. Meanwhile, in terms of phosphate solubility, based on the screening results, 6 out of 11 isolates were able to solubilize phosphate with an average dissolved phosphate concentration of 17.63 ppm on the 4th day of incubation. The results of the analysis of the effect of IAA-producing bacteria and phosphate solubilizers on the germination of green beans showed a significant effect on plant height, root length, fresh weight, and dry weight of green bean plants.
Isolation and Identification of IAA-Producing and Phosphate-Solubilizing Rhizobacteria from Sesuvium portulacastrum and In Vitro Growth-Promotion Effect on Vigna radiata L. Yuliana, Baiq Fadilla; Zulkifli, Lalu; Rasmi, Dewa Ayu Citra; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10070

Abstract

Sesuvium portulacastrum is a coastal plant resistant to high salt content and drought. Exploration of rhizosphere bacteria is necessary to obtain potential isolates that can be developed as biofertilizers in the future. This study aims to isolate and identify rhizosphere bacteria from Sesuvium portulacastrum that are able to produce Indole Acetic Acid (IAA) and dissolve phosphate, and to test their effects in vitro on the germination of Vigna radiata L. Rhizosphere bacteria from the Bagek Kembar Mangrove Ecosystem Area, Sekotong, West Lombok Regency. A total of 14 bacterial isolates were successfully obtained and characterized based on morphological, physiological, and biochemical properties. All isolates produced IAA qualitatively and quantitatively (19.85–33.71 ppm) with isolate KT1 having the highest production (33.71 ppm). Qualitative phosphate solubilization test showed that 12 isolates were able to form a clear zone, while quantitatively it ranged from 7.86 to 16.18 ppm with isolate K8 having the highest value (16.18 ppm). Selected isolates KT1 (the highest IAA producer), K8 (the highest phosphate solubilizer), and K5 (both with high abilities) were tested for their effect on mung bean germination. Inoculation had a significant effect on plant height, root length, and dry weight (p<0.05). These results indicate that S. portulacastrum rhizosphere bacteria have the potential to be an environmentally friendly biofertilizer for plants in high-salinity soils.
Analisys of Lead content in Tilapia (Oreochromis niloticus) from Taliwang Lake Khairuddin, Khairuddin; Yamin, M.; Kusmiyati, Kusmiyati; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.10772

Abstract

Research on heavy metals is important to be done to protect consumers from dangerous impact especially on human beings. The purpose of this study was to determine the Lead (Pb) content in Tilapia (Oreochromis niloticus) taken from Rawa Taliwang Lake. Specifically, the benefit of this study is to protect consumers who consume Tilapia from Lead heavy metal contaminants. Data collection for the tilapia species in this study was carried out in water bodies within the Taliwang Lake area. There were 2 data collection stations, namely on the east and west sides of the lake. Gill nets were used to catch tilapia. Fish samples were caught as many as 4 fish for each station. Next, each fish sample was put into a plastic bag and then stored in a sample box. The fish samples were then analyzed at the West Nusa Tenggara Health, Testing and Calibration Laboratory Center. The data analysis method was carried out by taking muscle tissue from the fish and then analyzing the Pb heavy metal content using an Atomic Absorption Spectrophotometer. The concentration of Lead in fish tissue was measured after adding concentrated Na2SO4 and CuSO4, which were heated at 350ºC for 2-3 hours until the solution was clear. The conclusion of this study is that the heavy metal content of Pb in Tilapia (Oreochromis niloticus) is <0.5 ppm.  
Measurement of Lead content in Channa striata by using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) Khairuddin, Khairuddin; Yamin, M.; Kusmiyati, Kusmiyati; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.11081

Abstract

Research on heavy metals Pb is important to be done to protect consumers from dangerous impact, especially on human beings. The purpose of this study was to determine the Lead (Pb) content in Snakehead fish (Channa striata) taken from Rawa Taliwang Lake. Data collection for the Snakehead fish (Channa striata) species in this study was carried out in water bodies of Taliwang Lake area. There were 2 data collection stations, namely on the east and west parts of the lake. Gill nets were used to catch fish. Fish samples were caught as many as 4 fish for each station. The fish samples were then analyzed at the West Nusa Tenggara Health, Testing, and Calibration Laboratory Center. The data analysis method was carried out by taking muscle tissue from the fish and then analyzing the Pb heavy metal content using an Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Pb found in Snakehead fish in this research is 0,44 ppm. The benefit of this study is to protect consumers who consume Snakehead fish from Lead heavy metal contaminants. The concentration of Lead in fish tissue was measured after adding concentrated 10 ml HNO3 and 2 ml H2O2 30%. The conclusion of this study is that the heavy metal content of Pb in Channa striata is 0.44 ppm.Reaseach on Pb heavy metal sould be continued in order to protect consumers from metal contamination.
Japanese Papaya (Cnidoscolus aconitifolius): An Integrated Biological System with Mechanism-Based Utilization Sedijani, Prapti; Mahrus, Mahrus; Rasmi, Dewa Ayu Citra; Kusmiyati, Kusmiyati; Khaerudin, Khaerudin
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.11234

Abstract

Cnidoscolus aconitifolius (Japanese papaya or chaya) is a tropical plant from the Euphorbiaceae family known for its high nutritional value and diverse biological activities. Several studies have reported antioxidant, antimicrobial, and other biological properties, suggesting the involvement of complex molecular systems. This article presents a conceptual literature review using a systems biology approach to understand Japanese papaya as an integrated biological system in which nutritional components, secondary metabolites, and enzymatic activities interact. By positioning hydrolytic enzyme systems as the central mechanism, this review discusses the potential utilization of Japanese papaya as a biological resource and as a basis for the development of functional derivative products, including biomolecular hydrolysates. Possible applications in mechanism-based bioconversion processes are also briefly addressed.
Meningkatkan Literasi Penulisan Karya Tulis Ilmiah Bagi Calon Guru IPA Melalui Pelatihan Gunawan, Gunawan; Sedijani, Prapti; Hakim, Aliefman
Indonesian Journal of Education and Community Services Vol. 2 No. 2 (2022): Edisi Juli-Desember 2022
Publisher : Indonesian Publication Center

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Karya tulis merupakan bagian utama dari bentuk penyampaian pengetahuan dan hasil kegiatan yang sudah dilaksanakan. Proses berbagi ilmu melalui tulisan berupa karya tulis ilmiah membutuhkan keterampilan yang sangat baik dalam penyusunannya. Oleh karena itu, dibutuhkan kemampuan literasi menulis yang tepat. Mahasiswa pada jenjang S1 ataupun S2 dituntut untuk bisa menghasilkan karya untuk melaporkan hasil penelitian yang telah dilaksanakan. Karya tulis yang berupa artikel ilmiah juga menjadi salah satu syarat kelulusan bagi mahasiswa calon guru. Namun, sebagian besar mahasiswa memiliki beberapa kendala dalam penuliasan karya tulis. Keterbatasan kemampuan menulis maupun kendala teknis lainnya dalam penulisan artikel ilmiah sehingga berimplikasi pada waktu kelulusan mahasiswa. Tujuan kegiatan ini adalah meningkatkan pengetahuan dan keterampilan mahasiswa calon guru IPA dalam menulis hingga mengirim naskah ke jurnal tujuan. Peserta diberikan pelatihan dan pendampingan secara intensif mulai dari pelatihan kemampuan dasar menulis artikel ilmiah hingga bimbingan teknis untuk submit ke jurnal tujuan dan revisi naskah hingga publish. Kegiatan pengabdian kepada masyarakat ini dilaksanakan dalam beberapa tahapan, yaitu 1) koordinasi dengan semua pihak terkait; 2) pelatihan tentang penulisan pada jurnal; 3) pendampingan dalam penulisan dan pengiriman artikel secara online; 4) focus group discussion pada beberapa masalah yang terjadi, solusi dan rencana tindak lanjutnya. Kegiatan ini dilaksanakan dengan menggunakan metode workshop dan dilanjutkan dengan bimbingan secara online. Pada kegiatan workshop disampaikan materi mengenai definisi artikel dan jurnal ilmiah, mengapa guru wajib memiliki kemampuan untuk meneliti dan menulis artikel, tata tulis artikel ilmiah serta alasan penolakan artikel di jurnal. Peserta pelatihan juga digabungkan dalam satu Whatsapp Group (WAG) dengan semua tim pelaksana untuk memudahkan koordinasi dan penyampaian informasi yang sifatnya penting dan segera. Dalam diskusi group ini juga peserta diizinkan menyampaikan pertanyaan dan mengirimkan draft manuskripnya melalui WAG untuk sama-sama dikoreksi oleh tim pengabdian maupun peserta pelatihan lainnya. Hasil pelatihan menunjukkan peningkatan kemampuan peserta dalam memahami tata cara menulis artikel ilmiah ke jurnal Nasional.
Measurement of Lead content in Claris batrachus from Taliwang Lake Khairuddin, Khairuddin; Yamin, M.; Kusmiyati, Kusmiyati; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.12419

Abstract

Catfish (Clarias batrachus) is one of the fish species that is an indicator of pollution in Lake Taliwang. This study aims to determine the levels of Lead (Pb) in the muscles of Catfish (Clarias batrachus). Consumers who consume Catfish exposed to Pb need to be protected from their health. Catfish were caught in the waters of Lake Taliwang using gill nets. There are 2 data collection stations, namely on the east and west sides of the lake. Fish samples were taken as many as 2 fish for each station. Furthermore, each fish sample was wrapped in a plastic bag and stored in a sample box. The fish samples were then analyzed at the West Nusa Tenggara Health, Testing and Calibration Laboratory Center. The data analysis method was carried out by taking muscle tissue from the fish and then analyzing the Pb heavy metal content using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). The conclusion of this study is that the Pb heavy metal content in Clarias batrachus is less than 0.5 ppm which indicates that the fish is still safe because the Pb level is still below the threshold according to applicable regulations in Indonesia.
Analysis of Mercury Heavy Metal Content in Snakehead, Tilapia and Catfish from Taliwang Lake Khairuddin, Khairuddin; Yamin, M.; Kusmiyati, Kusmiyati; Sedijani, Prapti
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.12582

Abstract

Research on heavy metals in fish is essential. This study aimed to determine the levels of the heavy metal mercury (Hg) in Snakehead (Channa striata), Catfish (Clarias batrachus), and Mozambique Tilapia (Oreochromis mosambicus) sampled from Lake Taliwang. The study is particularly valuable for protecting consumers from heavy metal contamination associated with fish consumption. The research was conducted at Lake Taliwang, West Sumbawa, with two observation stations established: one in the eastern section and one in the western section of the lake. The three fish species were caught using gill nets, with four samples collected for each species at each station. Each fish sample was placed in a plastic bag and stored in a sample storage box. The samples were subsequently analyzed at the West Nusa Tenggara Health Testing and Calibration Laboratory. Data analysis involved extracting fish muscle tissue and determining Hg content using a Mercury Analyzer. The wet digestion stage of the sample preparation process involved decomposing organic matter within the samples using strong acids specifically HNO3 and H2O2 as digesting agents. The data obtained represent Hg levels in mg/kg or ppm (parts per million). The final step involved descriptive processing of the obtained Hg concentrations and presenting the results in tabular form, based on the Mercury Analyzer findings. The study concluded that the Hg content in Snakehead (Channa striata), Catfish (Clarias batrachus), and Mozambique Tilapia (Oreochromis mosambicus) from Lake Taliwang ranged from 0.04 to 0.09 mg/kg.
Co-Authors A. Wahab Jufri Agil Al Idrus Ahmad Raksun, Ahmad Aliefman Hakim Aminatuzzifah, Putri Apriani, Yaumil Arifin, Ahmad Aris Ayuningtias, Wiranting Baiq Sri Handayani Desi, Petronela Eniarti, Miko Gifridus, Valeria Gunawan Gunawan Havizah, Ilma Nur Hidayati, Siti Nur Isnaini I Gde Mertha Ida Royani Ilhamdi, Mohammad Liwa Jamaluddin JANNATIN ARDHUHA, JANNATIN Japa , Lalu Karnan Karnan Khaerudin Khaerudin Khairuddin Khairuddin - Kusmiyati Kusmiyati Kusmiyati Kusuma, Nila Lalu Japa, Lalu LALU RUDYAT TELLY SAVALAS LALU ZULKIFLI Lestari, Chomsa Asih M. Yamin Mahrus Mahrus Mahrus, Mahrus Muhidayani, RE Septi Muhidayani, Respi Eka Muhlis Nafi'ah, Zidna Ilma Ni Luh Putu Pebri Artayani . Nurikhsanti, Mikiyal Priyo Hartanto Putra, Hendra Susana Putra, Masaradi Rahmi, Maskatur Rasmi, Dewa Ayu Citra Risnawati Sabila, Sabila Nur Amalina Sabrina, Yolanda Sania, Neza Okta Saprizal Hadisaputra Saputri, Andini Sari, Neti Kumala Sativa, Denianto Yoga Sukarso, Aa SUYANTRI, ENI Syamsul Bahri UMAMI, RIZAL Utami, Ni Wayan Anggun Diah Wahidah, Etty Nurul Yayuk Andayani Yuliana, Baiq Fadilla Zulkifili, Lalu